Mutants section of the slr0906 gene.
Genes and Mutants is a social mutants information repository. It allows biologist to submit a mutant information directly. This is a manual curated resource. You can contact the submitter by the e-mail address indicated.
MutantName indicates the mutant name.
GeneName indicates the gene name.
ProductName indicates the gene product name.
GeneticBackground indicates the genetic background: GT, Kazusa, PCC and etc...
DNA indicates the availability of the DNA material: yes or no.
DrugDirection indicates the direction of the drug marker: Fw (forward) or Rv (reverse).
DrugMarker indicates the construction of the drug maker.
AdditionalInformation indicates a free text of the mutant.
Post new mutant information of the slr0906 gene ... links to the submition form.
| Gene: psbB |
| Genes and Mutants | posted by Wim Vermaas <wim at asu.edu> at 1998-03-16 |
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| GeneName | psbB | | ProductName | CP47 protein of photosystem II | | Function | chlorophyll a-binding core antenna protein;the lumenal side of the protein helps determine the properties of the water-splitting system | | GeneticBackground | Not examined | | DNA | no | | Phenotype | yes | | PhenotypeDetails | Lacks photosystem II activity.Very much decreased amounts of D1 and D2; CP43 levels remain rather normal.Phenotype described in Vermaas et al. (1987, 1988, 1989); see below. | | AdditionalInformation | Insertion of Km cassette into Sma I site in psbB.. Vermaas, W.F.J., Williams, J.G.K., Rutherford, A.W., Mathis, P., and Arntzen, C.J. (1986) A genetically engineered mutant of the cyanobacterium Synechocystis 6803 lacks the photosystem II chlorophyll-binding protein CP47. Proc. Natl Acad. Sci. USA 83, 9474-9477.. Vermaas, W.F.J., Williams, J.G.K., and Arntzen, C.J. (1987) Sequencing and modification of psbB, the gene encoding the CP-47 protein of photosystem II, in the cyanobacterium Synechocystis 6803. Plant Mol. Biol. 8, 317-326.. Vermaas, W.F.J., Ikeuchi, M., and Inoue, Y. (1988) Protein composition of the photosystem II core complex in genetically engineered mutants of the cyanobacterium Synechocystis sp. PCC 6803. Photosynth. Research 17, 97-113.. Wim Vermaas. Department of Plant Biology, and. Center for the Study of Early Events in Photosynthesis. Arizona State University. Box 871601. Tempe, AZ 85287-1601, USA. phone: (602)965-3698. fax: (602)965-6899 |
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| Mutant: 14 different ones |
| Genes and Mutants | posted by Wim Vermaas <wim at asu.edu> at 1998-03-16 |
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| MutantName | 14 different ones | | GeneName | psbB | | ProductName | CP47 protein of photosystem II | | Function | light-harvesting core protein in photosystem II;The lumenal part of the protein contributes to the characteristics of the water-splitting system | | GeneticBackground | Not examined | | DNA | yes | | Phenotype | yes | | PhenotypeDetails | Fourteen mutants with short deletions in the lumenal loop of CP47.See Eaton-Rye and Vermaas (1991) and Haag et al. (1993) for details. | | AdditionalInformation | Deletions described in Eaton-Rye and Vermaas (1991):. G351-T365, and R384-V392.. Deletions described in Haag et al. (1993):. I265-F268, T271-K277, K277-E283, T304-L309, F311-N317, G333-I336,. K347-R352, A373-D380, V392-Q394, D416-F420, R422-E428, and D440-P447.. References:. Eaton-Rye, J.J., and Vermaas, W.F.J. (1991) Oligonucleotide-directed mutagenesis of psbB, the gene encoding CP47, employing a deletion mutant strain of the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol. Biol. 17, 1165-1177.. Haag, E., Eaton-Rye, J.J., Renger, G., and Vermaas, W.F.J. (1993) Functionally important domains of the large hydrophilic loop of CP47 as probed by oligonucleotide-directed mutagenesis in Synechocystis sp. PCC 6803. Biochemistry 32, 4444-4454.. Wim Vermaas. Department of Plant Biology, and. Center for the Study of Early Events in Photosynthesis. Arizona State University. Box 871601. Tempe, AZ 85287-1601, USA. phone: (602)965-3698. fax: (602)965-6899 |
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| Mutant: site-directed His mutations |
| Genes and Mutants | posted by Wim Vermaas <wim at asu.edu> at 1998-03-16 |
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| MutantName | site-directed His mutations | | GeneName | psbB | | ProductName | CP47 protein of photosystem II | | Function | light-harvesting core protein in photosystem II;The lumenal part of the protein contributes to the characteristics of the water-splitting system | | GeneticBackground | Not examined | | DNA | no | | Phenotype | yes | | PhenotypeDetails | Twenty-two mutants with mutations of conserved His residues in CP47.See Haag et al. (1993), Shen et al. (1993), and Shen and Vermaas (1994) for details.Results are consistent with His residues in hydrophobic regions of CP47 serving as axial chlorophyll ligands.His114 of CP47 is correlated specifically with the 695 nm fluorescence peak at 77K. | | AdditionalInformation | Site-directed mutation described in Haag et al. (1993):. H343L. Site-directed mutations in Shen et al. (1993):. H100Y, H114Y, H114N, H142N, H157Y, H201Y, H202Y, H216Y, H216N, H201Y/H202Y, H142N/H157N, H142N/H100Y, H216Y/H142Y, H201Y/H202Y/H157N, H201Y/H202Y/H157D, H201Y/H202Y/H216Y, and H201Y/H202Y/H216N. Site-directed mutations in Shen and Vermaas (1994): (all are in a PS I-less/apcE- background):. H9Y, H23Y, H23N, H26Y, H100Y, H114Q, H114N, H114Y, H142N, H157Y, H201Y, H202Y, H216Y, H216N, H142N/H157N, H201Y/H202Y, H201Y/H202Y/H157Q, and H201Y/H202Y/H216Y. References:. Haag, E., Eaton-Rye, J.J., Renger, G., and Vermaas, W.F.J. (1993) Functionally important domains of the large hydrophilic loop of CP47 as probed by oligonucleotide-directed mutagenesis in Synechocystis sp. PCC 6803. Biochemistry 32, 4444-4454.. Shen, G., Eaton-Rye, J.J., and Vermaas, W.F.J. (1993) Mutation of histidine residues in CP47 leads to destabilization of the photosystem II complex and to impairment of light energy transfer. Biochemistry 32, 5109-5115.. Shen, G., and Vermaas, W.F.J. (1994) Mutation of chlorophyll ligands in the chlorophyll-binding CP47 protein as studied in a Synechocystis sp. PCC 6803 photosystem I-less background. Biochemistry 33, 7379-7388.. Wim Vermaas. Department of Plant Biology, and. Center for the Study of Early Events in Photosynthesis. Arizona State University. Box 871601. Tempe, AZ 85287-1601, USA. phone: (602)965-3698. fax: (602)965-6899 |
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| Mutant: 20 combinatorial mutants |
| Genes and Mutants | posted by Wim Vermaas <wim at asu.edu> at 1998-03-16 |
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| MutantName | 20 combinatorial mutants | | GeneName | psbB | | ProductName | CP47 protein of photosystem II | | Function | light-harvesting core protein in photosystem II;The lumenal part of the protein contributes to the characteristics of the water-splitting system | | GeneticBackground | Not examined | | DNA | yes | | Phenotype | yes | | PhenotypeDetails | Twenty combinatorial, photoautotrophic mutants with random 8-residue sequences at residues 440-447 of CP47.Some mutants require an increased concentration of chloride for growth.See Tichy and Vermaas (1998) for details. | | AdditionalInformation | Amino acid sequences in the 440-447 region of CP47:. ERVGRGSA, KGVERMAL, VNLGRGGL, GNTGRLGV, LAGGRAAP, AGGSRGGA, NGEAREVT, GGRWGANS, GGRQGCGA, GGRLRNAV, HSVRGGSA, ESGRGATV, ERLWGEGT, ERVMASGP, EGLASDGV, DGYQGSAI, GDMSGGRL, REEWLVGV, WEGRGGSV, and VNNTASGV. Tichy, M., and Vermaas, W.F.J. (1998) Functional analysis of combinatorial mutants altered in a conserved region of loop E of the CP47 protein in Synechocystis sp. PCC 6803. Biochemistry 37, 1523-1531.. Wim Vermaas. Department of Plant Biology, and. Center for the Study of Early Events in Photosynthesis. Arizona State University. Box 871601. Tempe, AZ 85287-1601, USA. phone: (602)965-3698. fax: (602)965-6899 |
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| Mutant: chimaeric and replacement mutants |
| Genes and Mutants | posted by Wim Vermaas <wim at asu.edu> at 1998-03-16 |
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| MutantName | chimaeric and replacement mutants | | GeneName | psbB | | ProductName | CP47 protein of photosystem II | | Function | light-harvesting core protein in photosystem II;The lumenal part of the protein contributes to the characteristics of the water-splitting system | | GeneticBackground | Not examined | | DNA | yes | | Phenotype | yes | | PhenotypeDetails | Mutants with part or all of 6803 psbB replaced by the homologous part from spinach psbB.Replacement of particular regions of psbB with the spinach region leads to photoheterotrophic growth and a loss of PS II.See Vermaas et al. (1996) referenced below for more details. | | AdditionalInformation | See Vermaas, W.F.J., Shen, G., and Ohad, I. (1996) Chimaeric CP47 mutants of the cyanobacterium Synechocystis sp. PCC 6803 carrying spinach sequences: construction and function. Photosynth. Research 48, 147-162.. Wim Vermaas. Department of Plant Biology, and. Center for the Study of Early Events in Photosynthesis. Arizona State University. Box 871601. Tempe, AZ 85287-1601, USA. phone: (602)965-3698. fax: (602)965-6899 |
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