1
Wang, L.Sun, Y. P.Chen, W. L.Li, J. H.Zhang, C. C.
Genomic analysis of protein kinases, protein phosphatases and two-component regulatory systems of the cyanobacterium Anabaena sp. strain PCC 7120.
FEMS Microbiol Lett. 2002 Dec 17;217(2):155-65.
PMID:12480098
|
Abstract
|
MeSH
|
Related Articles
|
Sections
|
Gene Index
|
Anabaena sp. PCC 7120 is a cyanobacterium capable of performing several important biological functions: photosynthesis, nitrogen fixation, cell differentiation, cell-cell communication, etc. These activities require an extensive signaling capability in order to respond to the changing environment. Based on the genomic data, we have retrieved several gene families encoding signaling components. It is estimated that 211 genes encode two-component signaling elements, and 66 genes encode Ser/Thr kinases and phosphatases. These genes together represent 4.2% of the coding capacity of the whole genome, making Anabaena PCC 7120 a leading member among prokaryotes in terms of its signaling potential. It is known that two-component systems are composed of a few basic modules that can arrange into different structures best adapted for each signaling system. Many proteins in Anabaena PCC 7120 have incorporated both modules of two-component systems and catalytic domains of either Ser/Thr kinases or phosphatases. A family of 13 genes encode proteins with both a Ser/Thr kinase domain and a His kinase domain, and another four genes were also found whose products have both a response regulator domain and a Ser/Thr phosphatase domain. Of all the signaling proteins in Anabaena PCC 7120, about one third (35%) are conserved in the genome of the unicellular cyanobacterium strain Synechocystis sp. PCC 6803. Interestingly, one subfamily of His kinases and two subfamilies of response regulators are found in Anabaena PCC 7120 but are absent in Synechocystis PCC 6803. This study constitutes a basis for analyses of signal transduction in Anabaena PCC 7120 using functional genomic approaches.
MeSH terms
- Anabaena/*enzymology/genetics/metabolism
- Cyanobacteria/enzymology/genetics/metabolism
- *Genome, Bacterial
- Phosphoprotein Phosphatases/*genetics
- Phosphorylation
- Protein Kinases/*genetics/metabolism
- Protein-Serine-Threonine Kinases/metabolism
- Protein-Tyrosine Kinases/metabolism
- Sensitivity and Specificity
- *Signal Transduction/genetics
2
Ohmori, M.Ikeuchi, M.Sato, N.Wolk, P.Kaneko, T.Ogawa, T.Kanehisa, M.Goto, S.Kawashima, S.Okamoto, S.Yoshimura, H.Katoh, H.Fujisawa, T.Ehira, S.Kamei, A.Yoshihara, S.Narikawa, R.Tabat, S.
Characterization of genes encoding multi-domain proteins in the genome of the filamentous nitrogen-fixing Cyanobacterium anabaena sp. strain PCC 7120.
DNA Res. 2001 Dec 31;8(6):271-84.
PMID:11858227
|
Abstract
|
MeSH
|
Related Articles
|
Sections
|
Gene Index
|
Computational analysis of gene structures in the genome of Anabaena sp. PCC 7120 revealed the presence of a large number of genes encoding proteins with multiple functional domains. This was most evident in the genes for signal transduction pathway and the related systems. Comparison of the putative amino acid sequences of the gene products with those in the Pfam database indicated that and PAS domains which may be involved in signal recognition were extremely abundant in Anabaena: 87 GAF domains in 62 ORFs and 140 PAS domains in 59 ORFs. As for the two-component signal transduction system, 73, 53, and 77 genes for simple sensory His kinases, hybrid His kinases and simple response regulators, respectively, many of which contained additional domains of diverse functions, were presumptively assigned. A total of 52 ORFs encoding putative Hanks-type Ser/Thr protein kinases with various domains such as WD-repeat, GAF and His kinase domains, as well as genes for presumptive protein phosphatases, were also identified. In addition, genes for putative transcription factors and for proteins in the cAMP signal transduction system harbored complex gene structures with multiple domains.
MeSH terms
- Bacterial Proteins/*genetics
- Cluster Analysis
- Cyanobacteria/*genetics/metabolism
- *Genes, Bacterial
- Genome, Bacterial
- Multigene Family
- Nitrogen Fixation
- Phosphotransferases/genetics/metabolism
- Phylogeny
- Protein Structure, Tertiary/*genetics
- Signal Transduction
0.361924794 [0.172753463, 0.216172754, 0.331023022, 0.334856744, 0.357837675]
