BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_001458455.1P
2851 sequences; 968,037 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
apha_02493 Aminotransferase, class V 589 0.0
apha_02323 Nitrogenase metalloclusters biosynthesis protein nifS 272 4e-88
apha_02695 Aminotransferase, class V 253 1e-80
apha_03143 Cysteine desulfurase 212 4e-65
apha_02071 Cysteine desulfurase, SufS subfamily 87 1e-19
apha_02162 Aminotransferase, class V 61 3e-11
apha_01075 Aminotransferase, class V 34 0.008
>apha_02493 Aminotransferase, class V
Length = 382
Score = 589 bits (1519), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 287/380 (75%), Positives = 330/380 (86%), Gaps = 1/380 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTRPEAIA +Q L +QWGNPSSLHEWG RAA ++E R+QVA LI+A
Sbjct: 1 MQIYLDYSATTPTRPEAIAAMQVALKEQWGNPSSLHEWGQRAATVVEQGRIQVAKLIHAA 60
Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
PESIIFT+GGTEA+NLAIMGVAR YT PQH+IIS VEHSAISEP ++LE WGWE+TRLG
Sbjct: 61 NPESIIFTAGGTEANNLAIMGVARLYTVPQHIIISQVEHSAISEPVRLLEKWGWEVTRLG 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ +GRV+P DLK AL+ NTVLVS+IYGQSEIGTVQPIAELG IT+ H ALFHTDAVQ A
Sbjct: 121 VDSKGRVSPWDLKTALQDNTVLVSIIYGQSEIGTVQPIAELGNITQQHKALFHTDAVQAA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP+DV+ L VDLL+LSSHK+YG GAGALY+RPG EL+PLL GGGQE GLRSGTQA P
Sbjct: 181 GRLPIDVEKLSVDLLTLSSHKIYGPQGAGALYVRPGTELVPLLAGGGQEMGLRSGTQAVP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
IAGFGVAAELA+ EL ET RL+ LR RLFA LA++PGLIPTGD HRLPHH+SF +E
Sbjct: 241 VIAGFGVAAELASAELPGETPRLMKLRHRLFAQLANIPGLIPTGDLDHRLPHHVSFCVEH 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADG+K+SGK LVRQ+NLAGIGISAG+AC+SGKLSPSP+LLAMGYS+ AALGG+R TLG+
Sbjct: 301 ADGKKLSGKALVRQMNLAGIGISAGSACHSGKLSPSPVLLAMGYSESAALGGVRFTLGRD 360
Query: 360 TTAADIDWTGIVVKQILQRL 379
TTAAD+DW G+V +QILQRL
Sbjct: 361 TTAADVDWAGMVFEQILQRL 380
>apha_02323 Nitrogenase metalloclusters biosynthesis protein nifS
Length = 398
Score = 272 bits (696), Expect = 4e-88, Method: Compositional matrix adjust.
Identities = 159/378 (42%), Positives = 223/378 (58%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE + ++ L +GNPSS+H +G + A ++ AR Q+A LI A
Sbjct: 4 IYLDNNATTKVDPEVLEVMLPYLRDYYGNPSSMHTFGGQLAKPVKIARQQLAALIGADES 63
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I++TS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 64 EIVYTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYTVTYLSVNR 123
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ +L+AAL NT LV+++Y +E GTV PI ++G K +GA+FH D VQV G++
Sbjct: 124 QGQLDLNELEAALTGNTALVTIMYANNETGTVFPIEQIGLRVKEYGAIFHVDGVQVVGKI 183
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
P++++T +D+L+LS HK++ G GALY+R GV PLL GG QE G R+GT+ P I
Sbjct: 184 PMNMKTSTIDMLTLSGHKIHAPKGIGALYVRRGVRFRPLLIGGHQERGRRAGTENVPGII 243
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAEL LE T R LRDRL +LA +P GD RLP+ + + +
Sbjct: 244 ALGKAAELELLHLEEATARERKLRDRLEQTLLAKIPDCEVNGDLTQRLPNTTNIGFKYIE 303
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 304 GEAI-----LLSLNKYGICASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLSRYTT 358
Query: 362 AADIDWTGIVVKQILQRL 379
AA+I ++ I++RL
Sbjct: 359 AAEIHKVIEIMPGIVERL 376
>apha_02695 Aminotransferase, class V
Length = 393
Score = 253 bits (645), Expect = 1e-80, Method: Compositional matrix adjust.
Identities = 154/370 (41%), Positives = 221/370 (59%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP +A + T+ +GNPSS+ H +G A ++ AR +A INA P
Sbjct: 6 IYLDCHATTPVDERVLAAMIPYFTEHFGNPSSVSHIYGWEAEAAVKQAREILAAAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y QH+I + EHSA+ +P L++ G++IT L V
Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFQKGQHIITVATEHSAVLDPCNYLKSLGFDITVLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ L+ ALR T+LVSV+ +EIG +QP+A +G+I + +FH+DA Q G
Sbjct: 126 QPDGIIDLHQLETALRPETILVSVMAANNEIGVLQPLAAIGEICRAKNVIFHSDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++P++VQ + +DL+SL++HK+YG G GALY+R P V+L GGG E G+RSGT
Sbjct: 186 KIPINVQDMNIDLMSLTAHKVYGPKGIGALYVRRRNPRVQLAAQHHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A E+A +TE RL LR RL+ L+ + G+ G RL +L+ +
Sbjct: 246 TPQIVGFAKAVEIALATQDTENQRLTELRQRLWKQLSRLSGVYLNGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
DG + L L + IS+G+AC+S +PS +L A+G S+K A +R +
Sbjct: 306 PGVDGAAL-------LLGLQPVMAISSGSACSSKSTAPSHVLRALGRSEKLAYASVRFGI 358
Query: 357 GKQTTAADID 366
G+ TA ID
Sbjct: 359 GRFNTAEQID 368
>apha_03143 Cysteine desulfurase
Length = 389
Score = 212 bits (539), Expect = 4e-65, Method: Compositional matrix adjust.
Identities = 138/377 (36%), Positives = 214/377 (56%), Gaps = 27/377 (7%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD AT P PEA + I ++ GN S + G RAA I+ R +VA LI A P
Sbjct: 5 IYLDGFATLPLSPEARSAILSVWDHP-GNAGSPNAAGERAAGIIADGRAEVAALIGAAPG 63
Query: 63 SIIFTSGGTEADNLAIMGVARCYTT----PQHMIISSVEHSAISEPAKMLENWGWEITRL 118
IIFTSG TEA+NLA++G+A+ + +++S+VEH A+ EPA+MLE G+ ++
Sbjct: 64 EIIFTSGATEANNLALLGLAQSAVKGDFDRKRIVVSAVEHKAVLEPARMLEKQGFSVSIA 123
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
V+ +GR++ +A L + ++ SV+ +E G +QP+AE + G L H+DA Q
Sbjct: 124 PVDREGRLDLAAFEALLGDDVLIASVMLVNNETGVIQPVAEAAALVHAVGGLMHSDAAQA 183
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYL-----RPGVELMPLLGGGGQENGLRS 233
AG++ +DV L VD LSLS+HK YG +G G LY+ +P MPL+ GGGQ+ +R
Sbjct: 184 AGKIAIDVLDLEVDYLSLSAHKCYGPMGVGGLYVGAHAPKP----MPLVHGGGQQYAVRP 239
Query: 234 GTQATPAIAGFGVAAELAAQELETET--GRLILLR--DRLFAILADVPGLIPTGDKIHRL 289
GT+ IAGFG AA++A LE + GRL++ + +RL + + TGD +
Sbjct: 240 GTEPVALIAGFGAAAKVARSRLEGDDCHGRLLMCKFLERLASRQLRFSRI--TGDAA-VV 296
Query: 290 PHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAAL 349
P + +L D +++ T+ R ++L S G+AC SG++ S +L A+G S+ A
Sbjct: 297 PGSAALHLADVDADQLCA-TVARDVSL-----STGSACTSGQIKQSHVLEAIGLSEDDAR 350
Query: 350 GGIRLTLGKQTTAADID 366
IR+ + +++
Sbjct: 351 HVIRIFCHRYQNVDEVE 367
>apha_02071 Cysteine desulfurase, SufS subfamily
Length = 420
Score = 86.7 bits (213), Expect = 1e-19, Method: Compositional matrix adjust.
Identities = 68/230 (29%), Positives = 108/230 (46%), Gaps = 5/230 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ + +Q Q N H +A E AR ++A INA
Sbjct: 30 VYLDNAATSQKPLFVLNTLQHYYEQYNANVHRGAHSLSAKATDAYEAARNKIAKFINAAS 89
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITRL 118
+ I++T +EA NL P +I+S +EH + P + + + G + +
Sbjct: 90 RQEIVYTRNASEAINLVAYSWGMNNLQPGDEIILSVMEHHSNIVPWQFVSQKTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ +G + L+ + H T LV+V + + +G + P+AE+G I + A F DA Q
Sbjct: 150 ELTPEGCFDLPQLEKLISHQTKLVAVSHVSNTLGCINPVAEIGAIAHRYDAKFLIDACQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ 227
+P+DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 210 IPHMPVDVQQINCDWLVASGHKMCAPTGIGFLYGKLQLLESMPPFFGGGE 259
>apha_02162 Aminotransferase, class V
Length = 395
Score = 60.8 bits (146), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 62/225 (27%), Positives = 100/225 (44%), Gaps = 22/225 (9%)
Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRA-ALILETA---RMQVAGLI 57
+ Y +Y P +A+ I TQ + +GN A A ++E R +A +
Sbjct: 24 KFYFNYGGQGPMPQKAMNAI----TQAQADIQKAGPFGNEAFAKVIEAKQNLRAAMASEL 79
Query: 58 NAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHS-AISEPAKMLENWGWEIT 116
A PE+I T T N+A+ G+ + HM+IS EH I+ ++ + E+T
Sbjct: 80 QAPPETITLTEDVTVGCNIAMWGID--WRAGDHMLISDCEHPGVIAASQEIARRFAVEVT 137
Query: 117 ------RLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGAL 170
L +V + L R V+VS ++ + G V + ++ K+ + + L
Sbjct: 138 ICPLKQTLNAGDPVKVISQHLSPKTR--LVIVSHVFWNT--GQVLTLDKVVKLCRDNHTL 193
Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
DA Q G LPL++ L D + + HK L G G G LY+RP
Sbjct: 194 ILVDAAQSVGSLPLNLSELGADFYAFTGHKWLCGPAGVGGLYVRP 238
>apha_01075 Aminotransferase, class V
Length = 382
Score = 34.3 bits (77), Expect = 0.008, Method: Compositional matrix adjust.
Identities = 20/80 (25%), Positives = 41/80 (51%), Gaps = 4/80 (5%)
Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
++L+ A+ +H ++++++ ++ G QP+ + ++ + G L D V G +P+ +
Sbjct: 137 DELRTAVEKHRPAILALVHAETSTGARQPLEGVAELCREFGTLLLVDTVTSLGGVPIFLD 196
Query: 188 TLPVDLLSLSSHKLYGGLGA 207
VDL S K GLG
Sbjct: 197 AWGVDLAYSCSQK---GLGC 213
Database: GCA_001458455.1P
Posted date: Aug 27, 2016 10:51 PM
Number of letters in database: 968,037
Number of sequences in database: 2851
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2851
Number of Hits to DB: 747,565
Number of extensions: 32249
Number of successful extensions: 137
Number of sequences better than 1.0: 9
Number of HSP's gapped: 124
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 968,037
Length adjustment: 83
Effective length of query: 299
Effective length of database: 731,404
Effective search space: 218689796
Effective search space used: 218689796
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)