BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_001314905.1P
4158 sequences; 1,357,320 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HLUCCO16_07770 iscS-2 cysteine desulfurase 484 e-170
HLUCCO16_09140 iscS-3 cysteine desulfurase 278 3e-90
HLUCCO16_09790 nifS cysteine desulfurase NifS 277 7e-90
HLUCCO16_02305 iscS cysteine desulfurase 221 1e-68
HLUCCO16_15905 sufS bifunctional cysteine desulfurase / selenocy... 82 7e-18
HLUCCO16_20400 cefD L-cysteine/cystine lyase CefD 41 9e-05
HLUCCO16_11830 xseA exodeoxyribonuclease VII large subunit XseA 30 0.37
HLUCCO16_00135 hypothetical protein 29 0.43
HLUCCO16_15190 CHAT domain 28 0.68
>HLUCCO16_07770 iscS-2 cysteine desulfurase
Length = 419
Score = 484 bits (1247), Expect = e-170, Method: Compositional matrix adjust.
Identities = 250/383 (65%), Positives = 294/383 (76%), Gaps = 2/383 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLD+SATTPTRPEAI + L Q WGNPSSLH WG AA LETAR+QVA LI A
Sbjct: 36 MQIYLDHSATTPTRPEAIEAMMQSLHQAWGNPSSLHHWGETAATALETARLQVADLIAAP 95
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
PES++FTSGGTEA+NLA+MGV + P H+IISSVEH+AI++PA+ LE GW++T L V
Sbjct: 96 PESLVFTSGGTEANNLALMGVTQRLEQPGHLIISSVEHAAIAQPAQQLERQGWQVTYLPV 155
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N QGRV+P+ L+ A+R +T L+SVIYGQSE+GT+QPI L I HG LFHTDAVQVAG
Sbjct: 156 NGQGRVDPQGLETAIRPDTRLISVIYGQSEVGTLQPIDTLAAIAGQHGVLFHTDAVQVAG 215
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLPLDV L VDLLSLSSHKLYG GAGALY+RPGV L PLL GGGQE G RSGT+A
Sbjct: 216 RLPLDVAALGVDLLSLSSHKLYGPKGAGALYVRPGVSLTPLLAGGGQEAGRRSGTEAVAN 275
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFY--LE 298
+ GFGVAA LA EL E RL LRDRLF LA V L +GD+ HRLPHH+SF L+
Sbjct: 276 LVGFGVAATLAKAELAGEARRLAGLRDRLFEQLATVWSLQVSGDRQHRLPHHVSFMVDLD 335
Query: 299 AADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGK 358
A G+ ++GK LVRQLNLAGI IS+G+AC+SGKL+PSPILLAMGY ++ A GIRLTLG+
Sbjct: 336 DAQGKPVTGKALVRQLNLAGIAISSGSACHSGKLAPSPILLAMGYPERLARAGIRLTLGR 395
Query: 359 QTTAADIDWTGIVVKQILQRLIP 381
TT ADIDWT +V+KQ L +P
Sbjct: 396 DTTEADIDWTAMVLKQFLTGHLP 418
>HLUCCO16_09140 iscS-3 cysteine desulfurase
Length = 397
Score = 278 bits (711), Expect = 3e-90, Method: Compositional matrix adjust.
Identities = 155/370 (41%), Positives = 223/370 (60%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP +A + T+ +GNP+S+ H++G + AR +A INA P
Sbjct: 9 IYLDAHATTPVDERVLAAMLPYFTEYFGNPASINHQYGWEGEAAVTQARETLAAAINASP 68
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLA+ GVA Y + +H++ EH+AI +P L++ G+E+TRL V
Sbjct: 69 EEIIFTSGATEANNLALKGVAEAYFSKGRHLVTLVTEHNAILDPMDYLQSLGFEVTRLAV 128
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G V+ ++L AALR T+LVSV+ +EIG +QPIAE+G++ + G LFHTDA Q
Sbjct: 129 TSDGLVDLQELAAALREETILVSVMAANNEIGVLQPIAEIGQLCRDRGILFHTDAAQAIA 188
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ +DL+SL++HK+YG G GALY+R P V L L GGG E G+RSGT
Sbjct: 189 KIPLDVQAQSIDLMSLTAHKVYGPKGIGALYVRRRNPRVRLAAQLHGGGHERGMRSGTLY 248
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
P I GF A EL ++E E+ RL LR +L+ L+ + G+ G RLP +L+
Sbjct: 249 PPQIVGFAKAVELGLAQMEEESTRLRELRSQLWERLSVLEGIYLNGHATQRLPGNLNVSF 308
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG+ + L L + +S+G+AC S K+ PS +L A+G + A +R +
Sbjct: 309 EGVDGQAL-------LLGLQPVMAVSSGSACTSVKIEPSHVLAALGREESLAYASVRFGI 361
Query: 357 GKQTTAADID 366
G+ T +I+
Sbjct: 362 GRFNTLEEIN 371
>HLUCCO16_09790 nifS cysteine desulfurase NifS
Length = 403
Score = 277 bits (709), Expect = 7e-90, Method: Compositional matrix adjust.
Identities = 156/378 (41%), Positives = 218/378 (57%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE + TQ +GNPSS+H +G + A L +R +A LI+A
Sbjct: 4 IYLDNNATTCVAPEVYEAMSPYFTQFYGNPSSMHRFGGQVARGLRQSREAIAALIHAEAN 63
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
IIFTS GTE DN AI +H+I ++VEH AI +P + LE G+ +T LGV+
Sbjct: 64 EIIFTSCGTEGDNAAIRAALDSQPDKRHIITTAVEHPAILKPLQRLEKQGYSVTYLGVDG 123
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G++ E+L+ A+R +T L+SV+ +E GT+ PI +G + K G LFH DAVQ AG++
Sbjct: 124 KGQLKLEELRRAIRDDTALISVMAANNETGTIFPIERIGHLAKERGVLFHVDAVQAAGKI 183
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PLDV + +D L LS HK +G G G LY+R GV PLL GG QE G R GT+ P I
Sbjct: 184 PLDVSSGLIDFLVLSGHKFHGPKGVGVLYVRRGVRFRPLLAGGHQERGRRGGTENVPGII 243
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G G AAEL + + R+ LRDRL IL+ + G RLP+ + + +
Sbjct: 244 GLGKAAELTLNSFDEDHTRMSQLRDRLEQGILSTISDTQRNGHPGLRLPNTANIGFKYVE 303
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + L+ GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 304 GEAI-----LLSLDREGICASSGSACTSGSLQPSHVLRAMGLPYTLLHGSIRFSLSRYTT 358
Query: 362 AADIDWTGIVVKQILQRL 379
A+I+ V++ +++RL
Sbjct: 359 GAEIEQVLAVLQPMIERL 376
>HLUCCO16_02305 iscS cysteine desulfurase
Length = 380
Score = 221 bits (564), Expect = 1e-68, Method: Compositional matrix adjust.
Identities = 134/375 (35%), Positives = 204/375 (54%), Gaps = 11/375 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY ATTP I ++ LT +GN SS+ H +G+RAA ++TA+ +A L+ A P
Sbjct: 4 IYLDYHATTPVDRRVIEVMTETLTHHFGNASSIDHPYGDRAAAQVKTAKTHLATLLGASP 63
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW-EITRLGV 120
I+FTSG TE+ NL + GV P+ ++++ +EH A+ + + + G ++ L
Sbjct: 64 RDILFTSGATESINLVLQGV--LGDRPRRILLNPLEHKAVLDTCEAIAKRGQAQLHYLTP 121
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G+++ +D++ R L+ V+ +EIGT+ P+ + KI +T+G + DA Q G
Sbjct: 122 TATGQLHLDDIEHHCRQGIDLLCVMAANNEIGTIYPVEAIAKIAQTYGVPYLCDASQAVG 181
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
+LP + + L++S HK Y G GAL +RP L PL+ GGGQ+ G R GT P
Sbjct: 182 KLPFQFRDWGITFLTVSGHKFYAPQGCGALVVRPETPLTPLIYGGGQQQGRRPGTLNLPG 241
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAIL-ADVPGLIPTGDKIHRLPHHLSFYLEA 299
I G AA L E++ + R+ RDRL + L A +P L GD HRL +L +
Sbjct: 242 IVALGEAARLRTLEMQADESRIAHQRDRLQSHLQAKIPQLQVNGDLNHRLAGNLHISIPG 301
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
I + R + IS G+AC SG +PS +L A+G S++A G +R+ LGK
Sbjct: 302 IPNSAIIARVRDR------LAISTGSACTSGVETPSHVLRAIGLSEEAIEGSLRIGLGKF 355
Query: 360 TTAADIDWTGIVVKQ 374
T AD+D V+ Q
Sbjct: 356 TRDADLDEAATVLSQ 370
>HLUCCO16_15905 sufS bifunctional cysteine desulfurase / selenocysteine lyase SufS
Length = 420
Score = 82.0 bits (201), Expect = 7e-18, Method: Compositional matrix adjust.
Identities = 91/311 (29%), Positives = 139/311 (44%), Gaps = 35/311 (11%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-----LHEWGNRAALILETARMQVAGLI 57
VYLD +AT+ +P+A+ L+ + +S +H RA E R ++ I
Sbjct: 30 VYLDNAATS-QKPKAVI---DALSHYYSRDNSNVHRGVHALSARATDNYEGTRDKIVPFI 85
Query: 58 NAVPES-IIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWE 114
NA + I+FT +EA NL +I+S +EH + P +M+ + G
Sbjct: 86 NAESRNEIVFTRNASEAINLVAYSWGLNTLKAGDEIILSVMEHHSNLIPWQMVAQKTGAV 145
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G+ + E ++ L T LV+V++ + +G + P+ E+ ++ GA D
Sbjct: 146 LKFVGLTETQEFDFEQYQSLLSEKTKLVAVVHVSNTLGCINPVKEIAELAHRQGAKVLID 205
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ---ENG 230
A Q +P+DVQ + D L S HK+ G G LY + +E MP GGG+ +
Sbjct: 206 ACQSVPHMPIDVQDIDCDWLVASGHKMCAPTGIGFLYGKLALLESMPPFLGGGEMIADVE 265
Query: 231 LRSGTQA---------TPAIAGFGVAAEL-AAQELETETGRLIL------LRDRLFAILA 274
L S T A TPAI G A L AA + E G + L L+ LA
Sbjct: 266 LHSFTCAELPHKFEAGTPAI---GEAIALGAAIDYLMEIGMEAIADYEHDLCRHLYDKLA 322
Query: 275 DVPGLIPTGDK 285
VPGL G K
Sbjct: 323 TVPGLTLYGPK 333
>HLUCCO16_20400 cefD L-cysteine/cystine lyase CefD
Length = 391
Score = 40.8 bits (94), Expect = 9e-05, Method: Compositional matrix adjust.
Identities = 58/226 (25%), Positives = 89/226 (39%), Gaps = 18/226 (7%)
Query: 2 QVYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSL--HEWGNRAALILETARMQVAGLIN 58
+ YL+Y P AI AI Q Q P S + W A R +A +
Sbjct: 15 KTYLNYGGQGPLPQTAINAIRQAYEWVQRIGPFSAQANAWAQDCA---NQTRQAIAEELG 71
Query: 59 AVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE-NWGWEITR 117
++I T T N+A+ G+ + ++++ EH + + L+ +G E+
Sbjct: 72 VTADTIALTEDVTVGCNIALWGID--WQRGDRLVMTDCEHPGVVATVQELQYRFGVEVDL 129
Query: 118 LGVNHQGRVNP--EDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL----GKITKTHGALF 171
V N E + AL NT LV + + G V P+ E+ +++ G
Sbjct: 130 CPVQATLNTNNAVEVIAEALTANTRLVVLSHILWNTGQVLPLKEIVAACRQVSTRSGEPI 189
Query: 172 HT--DAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
DA Q G LPL + VD + + HK L G G G Y P
Sbjct: 190 RVLADAAQSVGVLPLALADSGVDYYAFTGHKWLCGPAGVGGFYCHP 235
>HLUCCO16_11830 xseA exodeoxyribonuclease VII large subunit XseA
Length = 417
Score = 29.6 bits (65), Expect = 0.37, Method: Compositional matrix adjust.
Identities = 34/149 (22%), Positives = 58/149 (38%), Gaps = 25/149 (16%)
Query: 10 TTPTRPEAIAIIQTILTQQWGN-----------------PSSLHEWGNRAALILETARMQ 52
+ P P+ IA++ + WG+ P+ + G+RAA +E A +
Sbjct: 140 SLPDHPQTIAVVTSPQAAAWGDIQSTLGQRYPGIRVLLSPTGVQ--GDRAAASIERAMAR 197
Query: 53 VAGLINAVPESIIFTSGGTEADNLAIMG---VARCYTTPQHMIISSVEHSAISEPAKMLE 109
V + E +I GG A++LA+ V R + +I+ + H A +
Sbjct: 198 VVA--DGRAELVILARGGGSAEDLAVFNDERVVRAVALCPYPVITGIGHQRDESLADLAA 255
Query: 110 NWGWEITRLGVNHQGRVNPEDLKAALRHN 138
++ E T Q + E L A RH
Sbjct: 256 DFVAE-TPTAAAKQAVPDLESLIEAHRHR 283
>HLUCCO16_00135 hypothetical protein
Length = 249
Score = 29.3 bits (64), Expect = 0.43, Method: Compositional matrix adjust.
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 3/56 (5%)
Query: 21 IQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNL 76
+Q I Q+WGNP +G+R + A +VAG VP G E+DNL
Sbjct: 181 VQRIAFQRWGNPDETG-YGDRLFSGVMDAETEVAGY--RVPSQFSVGWGQVESDNL 233
>HLUCCO16_15190 CHAT domain
Length = 207
Score = 28.5 bits (62), Expect = 0.68, Method: Compositional matrix adjust.
Identities = 21/90 (23%), Positives = 42/90 (46%), Gaps = 2/90 (2%)
Query: 293 LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGI 352
L+ YL+ A+GEK++ + LN+ + +AC +G + S +G S G
Sbjct: 53 LNAYLKLANGEKLTFLEIFNGLNIPLCRLLVLSACKTGLVETSHTDDYVGLSSAFFYAGA 112
Query: 353 RLTLGKQTTAADIDWTGIVVKQILQRLIPD 382
R + ++ T + ++ L +++PD
Sbjct: 113 RTVVASLWNVDELAATLVTLR--LYQILPD 140
Database: GCA_001314905.1P
Posted date: Aug 27, 2016 10:31 PM
Number of letters in database: 1,357,320
Number of sequences in database: 4158
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4158
Number of Hits to DB: 1,074,808
Number of extensions: 47386
Number of successful extensions: 162
Number of sequences better than 1.0: 10
Number of HSP's gapped: 153
Number of HSP's successfully gapped: 10
Length of query: 382
Length of database: 1,357,320
Length adjustment: 86
Effective length of query: 296
Effective length of database: 999,732
Effective search space: 295920672
Effective search space used: 295920672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)