BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_001314865.1P
4863 sequences; 1,652,793 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HLUCCA11_14790 iscS-3 cysteine desulfurase 480 e-169
HLUCCA11_03230 iscS cysteine desulfurase 242 3e-76
HLUCCA11_15470 nifS cysteine desulfurase NifS 223 9e-69
HLUCCA11_13300 iscS-2 cysteine desulfurase 75 2e-16
HLUCCA11_04635 sufS bifunctional cysteine desulfurase / selenocy... 73 9e-15
HLUCCA11_02760 L-cysteine/cystine lyase 56 2e-09
HLUCCA11_01860 alanine-glyoxylate transaminase / serine-glyoxyla... 38 0.001
HLUCCA11_13820 ddc L-2,4-diaminobutyrate decarboxylase 34 0.019
>HLUCCA11_14790 iscS-3 cysteine desulfurase
Length = 379
Score = 480 bits (1236), Expect = e-169, Method: Compositional matrix adjust.
Identities = 248/364 (68%), Positives = 300/364 (82%), Gaps = 7/364 (1%)
Query: 21 IQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA-VPESIIFTSGGTEADNLAIM 79
+Q + Q WGN SSLH+WGNR+A LE +R QVA LINA V ESIIFT GG+EA+NLAI
Sbjct: 1 MQVAMQQHWGNASSLHDWGNRSATALERSRQQVADLINASVAESIIFTGGGSEANNLAIF 60
Query: 80 GVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAALRHNT 139
GVA+ + PQH+IIS+VEHSAI+EPA+ LE GW++TRL V+ G ++P+DL+AALR NT
Sbjct: 61 GVAKQFLAPQHLIISAVEHSAITEPARQLERQGWQVTRLPVDRSGALHPKDLQAALRPNT 120
Query: 140 VLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSH 199
VLVS+IYGQSE+GT+QP+AELGKI + HG+LFHTDAVQVAGRLP++VQ LPVD LSLSSH
Sbjct: 121 VLVSIIYGQSEVGTLQPVAELGKIARAHGSLFHTDAVQVAGRLPINVQQLPVDFLSLSSH 180
Query: 200 KLYGGLGAGALYLRPGV---ELMPLLGGGGQENGLRSGTQATPAIAGFGVAAELAAQELE 256
K+YG GAGALY+ P V +L+P++ GGGQE GLRSGTQA I GFGVAAELAA E+
Sbjct: 181 KIYGPQGAGALYVSPHVSSAQLLPIIYGGGQERGLRSGTQAIANIVGFGVAAELAAAEMP 240
Query: 257 TETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNL 316
TET RLI LRDRLFA+L DVPGL+PTGD HRLPHH+SF L A+GE ++GKTLVRQ+NL
Sbjct: 241 TETLRLIRLRDRLFALLKDVPGLVPTGDSRHRLPHHVSFCL--AEGE-VTGKTLVRQMNL 297
Query: 317 AGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQIL 376
AGIGISAG+AC+SGKLSPSPIL AMG+S + A GIRLTLG+ T ADIDWT +V++Q+L
Sbjct: 298 AGIGISAGSACHSGKLSPSPILQAMGFSDRPATSGIRLTLGRHTAEADIDWTALVLQQVL 357
Query: 377 QRLI 380
R +
Sbjct: 358 SRTL 361
>HLUCCA11_03230 iscS cysteine desulfurase
Length = 409
Score = 242 bits (618), Expect = 3e-76, Method: Compositional matrix adjust.
Identities = 162/394 (41%), Positives = 225/394 (57%), Gaps = 27/394 (6%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD +ATTP P+ +A + T +GNPSSL H +G A ++ AR +A INA P
Sbjct: 9 IYLDCNATTPVVPQVLAAMLPFFTDYFGNPSSLNHSYGWEAEAGVDRARATIAAAINADP 68
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
I+FTSG TEA+NLAI GVA Y +HMI EHSA+ P + LE G+ +T L V
Sbjct: 69 AEIVFTSGATEANNLAIKGVAESYMAAGKHMITVQTEHSAVLAPCRYLETLGFSVTYLPV 128
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G V+ L+ A T+LVSV+ +EIG +QPI E+G++ + LFHTDA Q G
Sbjct: 129 GPDGLVDLSRLERAFCSETILVSVMAANNEIGVLQPIGEIGRMCRDRNILFHTDAAQALG 188
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYL------------------RPGVELMPLL 222
++PLDVQ + +DL+SL++HKLYG G GALY+ P V+L P L
Sbjct: 189 KIPLDVQAMNIDLMSLTAHKLYGPKGIGALYVGGRGVGRRNVDGRNVGGRNPRVQLSPQL 248
Query: 223 GGGGQENGLRSGTQATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPT 282
GGGQE GLRSGT P I GFG A LA E+ RL LRDRL+ L+ +P +
Sbjct: 249 HGGGQERGLRSGTLFPPQIVGFGEAVRLAMDTQTIESERLRRLRDRLWTHLSALPDIHLN 308
Query: 283 GDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMG 342
G RLP++L+ E +G + + +S+G+AC+ +PS ++ A+G
Sbjct: 309 GHPTKRLPNNLNVSFEGVEGSLLLSQLQSALA------LSSGSACSGANPTPSHVIKALG 362
Query: 343 YSQKAALGGIRLTLGKQTTAADIDWTG-IVVKQI 375
S + A +R LG++TT DID G +VV+++
Sbjct: 363 RSDRLARATLRFGLGQRTTEPDIDQAGSLVVEKV 396
>HLUCCA11_15470 nifS cysteine desulfurase NifS
Length = 405
Score = 223 bits (567), Expect = 9e-69, Method: Compositional matrix adjust.
Identities = 143/386 (37%), Positives = 212/386 (54%), Gaps = 12/386 (3%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
M +YLD +ATT P + + L+ +GNPSS+H +G + L+ AR QVA L+ A
Sbjct: 1 MVIYLDNNATTQIDPIVLQTMLPYLSDFYGNPSSMHRFGGQVGAALQDARAQVAELLGAE 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
I+F S G+E +N AI +H++ ++VEH AI K LE G+ +T L V
Sbjct: 61 TTEIVFNSCGSEGNNTAIHAALAAQPEKRHIVTTAVEHPAILNVCKHLEKKGYTVTYLSV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ +G+++ +++AA+ T LV+ +Y +E G + P+ ++G I K GA FH DAVQ G
Sbjct: 121 DGRGQLDLMEVEAAITGGTALVTTMYANNETGVIFPVEQIGAIAKECGATFHVDAVQAVG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
++P++++ +DLL+LS HKL+ G GALY+R G + GG QE G R+GT +
Sbjct: 181 KIPINLKNSTIDLLTLSGHKLHAPKGIGALYIRRGFRFRSFILGGHQERGRRAGTPNVAS 240
Query: 241 IAGFGVAAELAAQELETETGRLIL------LRDRLF-AILADVPGLIPTGDKIHRLPHHL 293
I G AAE+A Q L+T L LRD L ILA +P G HRLP+
Sbjct: 241 IVALGKAAEIAQQHLQTLDSSNPLNNPERQLRDLLEQQILATIPNCAVNGGGTHRLPNTT 300
Query: 294 SFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
+ + +GE I + L+ I S+G+AC+SG L PS +L AMG G IR
Sbjct: 301 NIGFKYIEGEAI-----LFMLDKHSICASSGSACSSGSLDPSHVLTAMGLPYTILHGSIR 355
Query: 354 LTLGKQTTAADIDWTGIVVKQILQRL 379
+L + TT DI V+ I+++L
Sbjct: 356 FSLSRFTTETDIQQVLTVLPTIVEKL 381
>HLUCCA11_13300 iscS-2 cysteine desulfurase
Length = 190
Score = 74.7 bits (182), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 51/147 (34%), Positives = 72/147 (48%), Gaps = 7/147 (4%)
Query: 220 PLLGGGGQENGLRSGTQATPAIAGFGVAAELAAQELETETGRLILLRDRL-FAILADVPG 278
PL+ GGGQ+ GLR GT P I G G A L E++ + + RD L + +PG
Sbjct: 30 PLMYGGGQQKGLRPGTLNVPGIVGLGEACRLRQLEMDDDERAIAAKRDHLQNRLFGSIPG 89
Query: 279 LIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPIL 338
L+ GD RL +L + + + VR + IS G+AC SG +PS +L
Sbjct: 90 LVVNGDPHTRLAGNLHIAIPGIPNSAVIAR--VRH----KLAISTGSACASGVEAPSHVL 143
Query: 339 LAMGYSQKAALGGIRLTLGKQTTAADI 365
AM + G +R+ LGK TT D+
Sbjct: 144 TAMSLPEDVIEGSLRIGLGKFTTDEDV 170
>HLUCCA11_04635 sufS bifunctional cysteine desulfurase / selenocysteine lyase SufS
Length = 424
Score = 72.8 bits (177), Expect = 9e-15, Method: Compositional matrix adjust.
Identities = 105/396 (26%), Positives = 164/396 (41%), Gaps = 55/396 (13%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
VYLD +AT+ +P+ + A++ +H RA E AR +VA +NA
Sbjct: 34 VYLDNAATS-QKPQVVIDAMVNYYAANNANVHRGVHALSARATDAYEGARDKVAAFVNAA 92
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCYTTPQH---MIISSVEH-SAISEPAKMLENWGWEI 115
+ IIFT +EA NL + A +T Q +++S +EH S + + + G +
Sbjct: 93 SRDEIIFTRNASEAINL--VAYAWGLSTLQQGDEIVLSVMEHHSNLVSWQLIAQRTGAVL 150
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + T LV+V + + +G + P+ ++ + +GA DA
Sbjct: 151 KYVPLTDTQEFDLAAFNQLVTDKTKLVAVSHVSNVLGCINPVQQIVETAHQYGAHVLIDA 210
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG--QENGL 231
Q + +DVQ L D L S HK+ G GAG LY + V + P LGGG Q+ L
Sbjct: 211 CQSVPHMRVDVQALGCDWLVASGHKMCGPTGAGFLYGKLDVLQAMPPFLGGGEMIQDVFL 270
Query: 232 RSGTQA---------TPAIA---GFGVAAELAAQ-ELETETGRLILLRDRLFAILADVPG 278
T A TPAIA G A + +Q ++ L L+ L+ VP
Sbjct: 271 DHSTYAELPHKFEAGTPAIAEAITLGAAVDYLSQIGMDRIADYEHQLTAYLYEQLSQVPQ 330
Query: 279 LIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGI--------SAGAACNSG 330
+ G K + ADG SG+ + + GI +G A SG
Sbjct: 331 VTLYGPKPN------------ADG---SGRAAIATFTVEGIHAQDLSTFLDQSGVAIRSG 375
Query: 331 KLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADID 366
P+ +G S A R +L T ++ID
Sbjct: 376 HHCTQPLHTVLGVSSTA-----RASLYFYNTPSEID 406
>HLUCCA11_02760 L-cysteine/cystine lyase
Length = 438
Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 74/268 (27%), Positives = 114/268 (42%), Gaps = 31/268 (11%)
Query: 4 YLDYSATTPTRPEAIAII-QTILTQQWGNPSS--LHEWGNRAALILETARMQVAGLINAV 60
Y +Y P AIA + Q T Q P S + W ++ + R +A +
Sbjct: 46 YFNYGGQGPMADRAIAALSQAQHTIQTEGPFSHAAYAWISQQG---QQVRGAIAHALGTQ 102
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHS-AISEPAKMLENWGWEITRLG 119
PE+I T T N+ + G+ + + H+++ EH ++ A++ +G E+T
Sbjct: 103 PETITLTENVTVGCNIPLWGLP--WQSGDHILMGESEHPGVVAAVAELSRRYGLEVTTCA 160
Query: 120 VNHQGRVNPED------LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173
+ D + LR NT LV + + G + P+ ++ + + H HT
Sbjct: 161 LAAARNATQADDGPVEIIAKGLRPNTRLVIISHILWNTGQLLPLTDI--VERCHRNAPHT 218
Query: 174 ----DAVQVAGRLPLDV--QTLP---VDLLSLSSHKLYGG-LGAGALYLRPGV--ELMPL 221
DA Q AG LPLD LP VD + + HK + G G GALY+RP V E P
Sbjct: 219 RVLVDAAQSAGSLPLDQPGWQLPETQVDYYAFTGHKWWCGPAGIGALYVRPEVLAETAPT 278
Query: 222 LGG--GGQENGLRSGTQATPAIAGFGVA 247
G G + +G + T P F VA
Sbjct: 279 FIGWRGIETDGAANPTGWKPDGQRFEVA 306
>HLUCCA11_01860 alanine-glyoxylate transaminase / serine-glyoxylate transaminase /
serine-pyruvate transaminase
Length = 389
Score = 38.1 bits (87), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 20/76 (26%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
Query: 126 VNPEDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPL 184
N +++AAL H ++++++ ++ G QP+ +G + + + L D V G +PL
Sbjct: 139 FNTSEIRAALETHRPAIMAIVHAETSTGVCQPMDGIGDLCREYECLLVLDTVTSLGGVPL 198
Query: 185 DVQTLPVDLLSLSSHK 200
+ VDL S K
Sbjct: 199 FLDKWGVDLAYSCSQK 214
>HLUCCA11_13820 ddc L-2,4-diaminobutyrate decarboxylase
Length = 500
Score = 34.3 bits (77), Expect = 0.019, Method: Compositional matrix adjust.
Identities = 26/104 (25%), Positives = 43/104 (41%), Gaps = 10/104 (9%)
Query: 78 IMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAAL-- 135
I+G+AR +S V H+++ + A M+ + N +++ +DL A L
Sbjct: 175 IVGLARQPV----FFVSEVAHTSMQKAAMMMGLGSQAAIAVRTNANSQMDLDDLTAKLAQ 230
Query: 136 ----RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ G + G + PIAE+ I H FH DA
Sbjct: 231 AKTANQQPFALVATAGTTVTGNIDPIAEMSAIAHAHRLWFHVDA 274
Database: GCA_001314865.1P
Posted date: Aug 27, 2016 10:47 PM
Number of letters in database: 1,652,793
Number of sequences in database: 4863
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4863
Number of Hits to DB: 1,272,524
Number of extensions: 54634
Number of successful extensions: 181
Number of sequences better than 1.0: 9
Number of HSP's gapped: 171
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,652,793
Length adjustment: 87
Effective length of query: 295
Effective length of database: 1,229,712
Effective search space: 362765040
Effective search space used: 362765040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)