BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000828075.1P 7886 sequences; 2,436,924 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SD81_23175 cysteine desulfurase 596 0.0 SD81_18285 cysteine desulfurase 296 3e-97 SD81_19045 cysteine desulfurase 268 4e-86 SD81_19370 cysteine desulfurase 77 4e-16 SD81_10470 cysteine desulfurase 74 4e-15 SD81_02590 aminotransferase 70 6e-14 SD81_33140 cysteine lyase 61 5e-11 SD81_06450 alanine--glyoxylate aminotransferase 35 0.017 SD81_21560 glutamate decarboxylase 33 0.057 SD81_41055 hypothetical protein 29 0.95 3e5579676f98bf2edd44bc4a5e02ff80 >SD81_23175 cysteine desulfurase Length = 389 Score = 596 bits (1537), Expect = 0.0, Method: Compositional matrix adjust. Identities = 288/382 (75%), Positives = 333/382 (87%), Gaps = 1/382 (0%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 MQ+YLDYSATTPTRPEAIA +Q + TQQWGNPSSLHEWG RAA ++E ARMQVA LINA Sbjct: 1 MQIYLDYSATTPTRPEAIAAMQAVFTQQWGNPSSLHEWGQRAATVVEQARMQVAPLINAP 60 Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 PESIIFTSGGTEA+NLAIMGVA Y PQH+IIS+VEHSA++EP ++LE WGW++TRLG Sbjct: 61 DPESIIFTSGGTEANNLAIMGVAHLYAKPQHIIISNVEHSAVTEPVRLLELWGWQVTRLG 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 V+ +GRVNP DLKAAL+ NTVLVSVIYGQSE+GT+QPI ELG I ++ G LFHTDAVQVA Sbjct: 121 VDAKGRVNPLDLKAALQDNTVLVSVIYGQSEVGTLQPIEELGNIARSAGVLFHTDAVQVA 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 GRLP++V+ PVDLLS+SSHK+YG GAGALY+RPGVEL+PLL GGGQE LRSGTQA P Sbjct: 181 GRLPVNVKNTPVDLLSISSHKIYGPQGAGALYVRPGVELLPLLNGGGQEKRLRSGTQAVP 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 +AGFGVAAELA QE+ TET RL+ LRDRLFA L D+PGLIPTGD HRLPHH+SF LE Sbjct: 241 ILAGFGVAAELATQEMPTETPRLMKLRDRLFAQLRDIPGLIPTGDCAHRLPHHVSFCLEY 300 Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359 ADGEK+SGKTLVRQ+NLAGIGISAGAAC+SGKLSPSPILLAMGY + AALG IR+TLG++ Sbjct: 301 ADGEKLSGKTLVRQMNLAGIGISAGAACHSGKLSPSPILLAMGYPENAALGAIRMTLGRE 360 Query: 360 TTAADIDWTGIVVKQILQRLIP 381 TT AD+DW +V++QIL+RL P Sbjct: 361 TTEADVDWVAMVLRQILERLSP 382 >SD81_18285 cysteine desulfurase Length = 392 Score = 296 bits (759), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 167/369 (45%), Positives = 230/369 (62%), Gaps = 11/369 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLD ATTP +A + T+ +GNPSS+ H +G A ++ R +A INA P Sbjct: 6 IYLDCQATTPVDERVLAAMLPFFTEHFGNPSSISHVYGWEAEGAVKQTREILAPAINATP 65 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E I+FTSG TEA+NLAI GVA Y QH+I + EHSA+ +P K L+N G+E+T L V Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFQKGQHIITVATEHSAVLDPCKYLKNLGFEMTFLSV 125 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 G ++ +L+ A R +T+LVSV+ +EIG +QP+AE+G + + ALFHTDA Q G Sbjct: 126 QKDGLIDLTELEKAFRPDTILVSVMAANNEIGVLQPLAEIGAMCRERNALFHTDAAQAIG 185 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237 ++PLDVQ L +DL+SL++HK+YG G GALY+R P V+L P GGG E G+RSGT Sbjct: 186 KIPLDVQALKIDLMSLTAHKVYGPKGIGALYVRRRNPRVQLAPQQHGGGHERGMRSGTLY 245 Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297 TP I GFG A E+A Q+ TET RL LR RL+ L+ V G+ G RLP +L+ + Sbjct: 246 TPQIVGFGKAVEIALQQQATETERLTQLRQRLWEQLSQVEGIHLNGHPTQRLPGNLNISV 305 Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357 E DG S L Q +A +S+G+AC+S +PS +L A+G+S+ A IR +G Sbjct: 306 EGVDG---SALLLGLQPVMA---VSSGSACSSATTAPSHVLTALGHSEALAYASIRFGIG 359 Query: 358 KQTTAADID 366 + T +ID Sbjct: 360 RFNTDEEID 368 >SD81_19045 cysteine desulfurase Length = 403 Score = 268 bits (685), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 160/378 (42%), Positives = 221/378 (58%), Gaps = 6/378 (1%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +YLD +ATT PE I + L +GNPSS+H +G + + AR QVA L+ A Sbjct: 6 IYLDNNATTKVDPEVIEAMLPYLRDYYGNPSSMHTFGGQVGKAVRQAREQVAALLGADES 65 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122 IIFTS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN Sbjct: 66 EIIFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYSVTYLSVNR 125 Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182 QG+++ ++L+A+L NT LV+++Y +E GTV PI ++G K GALFH DAVQ G++ Sbjct: 126 QGQLDLDELEASLTGNTALVTIMYANNETGTVFPIEQIGLRAKERGALFHVDAVQAVGKI 185 Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242 PL+++T +D+L++S HK++ G GALY+R GV PLL GGGQE G R+GT+ P I Sbjct: 186 PLNMKTSTIDMLTMSGHKIHAPKGIGALYVRRGVRFRPLLIGGGQERGRRAGTENVPGIV 245 Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301 G AAEL LE T R LRDRL ++A +P GD RLP+ + + + Sbjct: 246 ALGKAAELELFHLEEATTREKRLRDRLEQTLIATIPDCEVNGDPTQRLPNTTNIGFKYIE 305 Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361 GE I + LN I S+G+AC+SG L PS +L AMG G IR +L + TT Sbjct: 306 GEAI-----LLHLNKHNICASSGSACSSGSLEPSHVLRAMGLPYTILHGSIRFSLCRYTT 360 Query: 362 AADIDWTGIVVKQILQRL 379 A+ID V+ +++RL Sbjct: 361 EAEIDAVLSVMPSVVERL 378 >SD81_19370 cysteine desulfurase Length = 415 Score = 77.4 bits (189), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 66/231 (28%), Positives = 105/231 (45%), Gaps = 10/231 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNP--SSLHEWGNRAALILETARMQVAGLINAV 60 VY D +A + +P + +Q+ + S H A E AR ++A +NA Sbjct: 30 VYFD-NAESSHKPSVVLNALRDYYEQYNSNVHQSTHVLAKEATEAYEAAREKLAKFVNAA 88 Query: 61 -PESIIFTSGGTEADNLAIM--GVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEIT 116 P+ II+T TE NL G+ TT +IIS +EH + P +++ + G + Sbjct: 89 SPQEIIYTRNATEGINLVAYDWGINNL-TTGDEIIISVMEHHSNIVPWQVVAQKTGAVLK 147 Query: 117 RLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAV 176 + +N N E K + T LVSV + S +G + P+ E+ I + G+ D Sbjct: 148 FVELNENEEFNLEQFKTLISDKTKLVSVSHLSSTLGCINPVEEIIAIARQKGSKVLIDTC 207 Query: 177 QVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225 Q + +DVQ + D L S +K+ G G G LY + + + P L GG Sbjct: 208 QSLPHMSIDVQKMDCDWLVGSGYKMCGPTGIGLLYGKSELLRSIPPFLTGG 258 >SD81_10470 cysteine desulfurase Length = 420 Score = 74.3 bits (181), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 62/232 (26%), Positives = 109/232 (46%), Gaps = 12/232 (5%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61 +Y D +AT+ + ++ Q N +H +A E +R +VA +NA Sbjct: 30 IYFDNAATSQKPLLVLNTLRDYYEQYNSNVHRGVHILSAKATDAYEASRDKVAAFVNAAS 89 Query: 62 -ESIIFTSGGTEADNLAIM--GVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITR 117 + I+FT +EA NL G++ + +I+S +EH + P + + G + Sbjct: 90 RQEIVFTRNASEAINLVAYSWGMSNLQRGDE-IILSVMEHHSNLVPWHFVAQKTGAVLKF 148 Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177 + + + + E K + T LVS+++ + +G + P+ E+ +I +GA DA Q Sbjct: 149 VELAAEETFDLEQFKKLISDKTKLVSLVHVSNTLGCINPVKEICEIAHKYGAKVLIDACQ 208 Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELM----PLLGGG 225 +P++VQ + D L S HK+ G G LY + +EL+ P LGGG Sbjct: 209 SVPHMPINVQQIDCDWLVASGHKMCAPTGIGFLYGK--LELLRSMPPFLGGG 258 >SD81_02590 aminotransferase Length = 413 Score = 70.5 bits (171), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 50/212 (23%), Positives = 88/212 (41%), Gaps = 2/212 (0%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 ++ D T I + L + N R ++ +AR +A L+ + Sbjct: 26 IFFDGPGGTQVPSSVIDALAEYLVKSNANAHGAFATSQRTDTLITSARAAIADLLGCNSD 85 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN- 121 ++F + T +AR +I++ ++H A P L G I + +N Sbjct: 86 EVVFGANMTTLTYTLSRAIARELQPGDEIIVTKLDHYANVSPWYALSERGVVIREVDINV 145 Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181 +N L+ + T LV+V Y + +GT+ +A + K+ GA+ DAV Sbjct: 146 EDCTLNMNHLQQQINERTRLVAVGYASNAVGTINDVATVVKLAHAVGAMVFVDAVHYVAH 205 Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR 213 P+DV L D L+ S++K +G G LY + Sbjct: 206 APIDVHMLECDFLACSAYKFFGP-HVGVLYAK 236 >SD81_33140 cysteine lyase Length = 396 Score = 61.2 bits (147), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 64/226 (28%), Positives = 104/226 (46%), Gaps = 20/226 (8%) Query: 2 QVYLDYSATTP---TRPEAIAIIQTILTQ--QWGNPSSLHEWGNRAALILETARMQVAGL 56 + Y +Y P T +AI+ Q + Q +GN E G ++ AR+ +A Sbjct: 24 KTYFNYGGQGPMPQTALDAISHAQAYIQQIGPFGN-----EVGRWVGQEVKAARVAIASE 78 Query: 57 INAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115 +N E+I T T N+A+ G+ + H+++S EH I A+ + + E+ Sbjct: 79 LNVPAETITLTEDVTVGCNIAMWGLD--WQPGDHILLSDCEHPGIIATAQEIGRRFAVEL 136 Query: 116 TR---LGVNHQGRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALF 171 + + ++G NP E + LR T LV + + G V PI ++ +I + + Sbjct: 137 STCPLMATLNEG--NPVEVITQHLRPKTRLVILSHVLWNTGQVLPIDKIAEICRDNSIKL 194 Query: 172 HTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRPGV 216 DA Q G LPL++ L D + + HK L G G G LY+RP V Sbjct: 195 LIDAAQSVGLLPLNLTELGADFYAFTGHKWLCGPEGVGGLYVRPEV 240 >SD81_06450 alanine--glyoxylate aminotransferase Length = 383 Score = 34.7 bits (78), Expect = 0.017, Method: Compositional matrix adjust. Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 4/80 (5%) Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187 ++L+ AL H ++++++ ++ G QP+ +G + + G L D V G +P + Sbjct: 137 DELRTALETHRPAILALVHAETSTGARQPLEGVGDLCREFGTLLLVDTVTSLGGVPFLLD 196 Query: 188 TLPVDLLSLSSHKLYGGLGA 207 VD+ S K GLG Sbjct: 197 AWGVDVAYSCSQK---GLGC 213 >SD81_21560 glutamate decarboxylase Length = 554 Score = 33.1 bits (74), Expect = 0.057, Method: Compositional matrix adjust. Identities = 29/133 (21%), Positives = 62/133 (46%), Gaps = 13/133 (9%) Query: 94 SSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAAL----RHNTVLVSV--IYG 147 S + H + + A ++ + R+ V+ RVN + L+ A+ + N ++++ I G Sbjct: 234 SELMHFSFDKAADLMGISTQNLIRVPVDSNNRVNIQALRQAVAECRKQNLHIIAIVGIAG 293 Query: 148 QSEIGTVQPIAELGKITKTHGALFHTDA-----VQVAGRLPLDVQTLP-VDLLSLSSHK- 200 ++ G+V + + I + + FH DA V + + + + D +++ HK Sbjct: 294 TTDSGSVDSLENIADIAQENNIYFHVDAAWGGPVMFSEKHRHKLAGIERADSVTIDGHKQ 353 Query: 201 LYGGLGAGALYLR 213 LY +G G ++ R Sbjct: 354 LYLPMGIGMVFFR 366 >SD81_41055 hypothetical protein Length = 363 Score = 29.3 bits (64), Expect = 0.95, Method: Compositional matrix adjust. Identities = 34/104 (32%), Positives = 44/104 (42%), Gaps = 11/104 (10%) Query: 169 ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-----ELMPLLG 223 AL H D V + +D+Q + +L +L GL RP V L+G Sbjct: 114 ALEHGDQV-----VAVDLQAVQPAGQALLRERLRAGLRRTRHRDRPAVVDHAQHQRELVG 168 Query: 224 GGGQENGLRSG-TQATPAIAGFGVAAELAAQELETETGRLILLR 266 G E G+ G +A A AG G A LA E E GR+ LR Sbjct: 169 AAGIERGVEVGLRRAAVAAAGHGDAVLLAQLERERRAGRVQALR 212 Database: GCA_000828075.1P Posted date: Aug 27, 2016 11:17 PM Number of letters in database: 2,436,924 Number of sequences in database: 7886 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 7886 Number of Hits to DB: 1,877,240 Number of extensions: 81408 Number of successful extensions: 268 Number of sequences better than 1.0: 12 Number of HSP's gapped: 259 Number of HSP's successfully gapped: 12 Length of query: 382 Length of database: 2,436,924 Length adjustment: 89 Effective length of query: 293 Effective length of database: 1,735,070 Effective search space: 508375510 Effective search space used: 508375510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)
1.02492495