BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000817785.1P
10,181 sequences; 3,132,640 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PI95_09450 cysteine desulfurase 615 0.0
PI95_03445 cysteine desulfurase 283 6e-92
PI95_42025 cysteine desulfurase 250 3e-79
PI95_53745 cysteine desulfurase 243 3e-76
PI95_22460 cysteine desulfurase 188 1e-55
PI95_34740 cysteine sulfinate desulfinase 93 2e-21
PI95_11630 cysteine desulfurase 78 3e-16
PI95_04400 cysteine lyase 58 8e-10
PI95_26510 aminotransferase class V 33 0.069
PI95_47140 penicillin epimerase 33 0.080
>PI95_09450 cysteine desulfurase
Length = 382
Score = 615 bits (1585), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 300/380 (78%), Positives = 338/380 (88%), Gaps = 1/380 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTR EAIA +Q ILTQQWGNPSSLHEWG RAA I+E ARMQVAGLINAV
Sbjct: 1 MQIYLDYSATTPTRSEAIATMQAILTQQWGNPSSLHEWGQRAATIVEQARMQVAGLINAV 60
Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
P+SIIFTSGGTEADNLAIMG+AR Y PQHMIISSVEHSAISEPA++LE WGW+ITRL
Sbjct: 61 DPQSIIFTSGGTEADNLAIMGIARLYAVPQHMIISSVEHSAISEPARLLEMWGWQITRLP 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ +G VNP DL+AAL+ NTVL+SVIYGQSE+GTVQPIAELG I ++H +FHTDAVQVA
Sbjct: 121 VDAKGTVNPVDLEAALQPNTVLISVIYGQSEVGTVQPIAELGNIARSHNVIFHTDAVQVA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP++VQTL VDLLSLSSHK+YG GAGALY+RPGV+L PLL GGGQE LRSGTQA P
Sbjct: 181 GRLPINVQTLAVDLLSLSSHKIYGCQGAGALYVRPGVDLAPLLSGGGQEMRLRSGTQAVP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
IAGFGVAAELA QE+ TET RLI LRDRLF+ LAD+PGLIPTGD I+RLPHH+SF L+
Sbjct: 241 NIAGFGVAAELATQEIVTETPRLIKLRDRLFSQLADIPGLIPTGDMIYRLPHHVSFCLQY 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADGEK+SGKTLVR +NLAGIGISAGAACNSGKLSPSPILLAMGYS+KAALG IR++LG++
Sbjct: 301 ADGEKLSGKTLVRMMNLAGIGISAGAACNSGKLSPSPILLAMGYSEKAALGAIRMSLGRE 360
Query: 360 TTAADIDWTGIVVKQILQRL 379
TT+AD+DW +V KQILQRL
Sbjct: 361 TTSADVDWVAMVFKQILQRL 380
>PI95_03445 cysteine desulfurase
Length = 388
Score = 283 bits (724), Expect = 6e-92, Method: Compositional matrix adjust.
Identities = 156/370 (42%), Positives = 228/370 (61%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP +A + TQ +GNPSS+ H +G A ++ R +A INA P
Sbjct: 6 IYLDCHATTPVDERVLAAMLPYFTQHFGNPSSISHVYGWEAEAAVKQTRSILAEAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y QH+I + EHSA+ +P L++ G+E+T L V
Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYYQKGQHIITVATEHSAVIDPCNYLKSLGFEVTILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G +N L+ ALR +T+LVSV+ +EIG +QP+AE+G++ ++ +FHTDA Q
Sbjct: 126 EKDGIINLNQLEKALRPDTILVSVMAANNEIGVLQPLAEIGELCRSQEIIFHTDAAQAIA 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ + +DL+SL++HK+YG G GA+Y+R P V+L GGG E G+RSGT
Sbjct: 186 KIPLDVQAMKIDLMSLTAHKVYGPKGIGAIYVRRRNPRVQLAAQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A +E + E RL LR+RL+ L+ +PG+ G RL +L+ +
Sbjct: 246 TPQIVGFGKAVEIALEEQKIECDRLTQLRERLWEQLSQLPGIHLNGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L + +S+G+AC+S +PS +L A+G+S++ A +R +
Sbjct: 306 EGVDGAALL-------LGLQPVMAVSSGSACSSVTTAPSHVLTALGHSEQLAYASVRFGI 358
Query: 357 GKQTTAADID 366
G+ T +ID
Sbjct: 359 GRFNTQEEID 368
>PI95_42025 cysteine desulfurase
Length = 382
Score = 250 bits (638), Expect = 3e-79, Method: Compositional matrix adjust.
Identities = 143/382 (37%), Positives = 212/382 (55%), Gaps = 12/382 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD ++TT + + + L Q+ N SS HE+G ++ AR VAGLI
Sbjct: 2 IYLDNNSTTKLDAKVLEAMLPFLQDQYANASSNHEFGVSVNSKIKDARSSVAGLIGCEAN 61
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
I+FTSG TEA NLAI GVA Y++ +H+I + EH A+ + K LE G+EI+ L V+
Sbjct: 62 EIVFTSGATEAINLAIKGVAENYSSKGKHIITVATEHPAVLDTCKYLERIGFEISYLPVD 121
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
G ++ + ++ LR +T+L+SV+ +E G +QP+AE+ K+ + G + TD Q G+
Sbjct: 122 SNGLIDLKQVEKTLRDDTILISVMLVNNETGVIQPLAEISKLARKKGIVVMTDGTQAVGK 181
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR----PGVELMPLLGGGGQENGLRSGTQA 237
LP+ V L VDL++ S HK YG G GAL++R V + P+L GGG E LRSGT
Sbjct: 182 LPVSVDELGVDLMAFSGHKFYGPKGVGALFVRNRRPNKVRIEPILHGGGHERNLRSGTLN 241
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
P I G GVAAE+A E+E + LLR+ L L + G G K RL + +
Sbjct: 242 VPGIVGLGVAAEIAQNEMEQNRKHIGLLREMLEVELLKIEGAKLNGSKNKRLYNISNVSF 301
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
+ D + I L + +S G+AC+S K+ PS +L MG S++ A +R ++G
Sbjct: 302 KDIDADAIIA-------GLENVIVSNGSACSSNKVEPSHVLTQMGLSEQDAYSSVRFSIG 354
Query: 358 KQTTAADIDWTGIVVKQILQRL 379
K T +DI K I+++L
Sbjct: 355 KFNTKSDITKAIQKTKAIVEKL 376
>PI95_53745 cysteine desulfurase
Length = 400
Score = 243 bits (620), Expect = 3e-76, Method: Compositional matrix adjust.
Identities = 158/378 (41%), Positives = 221/378 (58%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE + + +T +GNPSS+H +G + ++ AR QVA L+ A
Sbjct: 6 IYLDNNATTKVDPEVVEAMMPYMTDFYGNPSSMHTFGGQVGKAVKQARQQVAALLGADDS 65
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
IIFTS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 66 EIIFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYTVTYLSVNG 125
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G+++ ++L+A+L NT LV+++Y +E G + PI ++G K +GA+FH DAVQ G++
Sbjct: 126 KGQLDLDELEASLTGNTALVTIMYANNETGVIFPIEQIGLRVKEYGAIFHVDAVQAVGKI 185
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T +D+L++S HK++ G G LY+R GV P L GG QE G RSGT+ P I
Sbjct: 186 PLNMKTSTIDMLTMSGHKIHAPKGIGVLYVRRGVRFRPFLIGGQQERGRRSGTENVPGII 245
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAEL LE T R LRDRL AIL +P GD RLP+ + + +
Sbjct: 246 ALGKAAELELLHLEEATKRERRLRDRLEQAILTKIPDCEVNGDPTQRLPNTTNIGFKYIE 305
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC+SG L PS +L AMG G IR +L + TT
Sbjct: 306 GEAI-----LLYLNQHGICASSGSACSSGSLEPSHVLRAMGLPYTILHGSIRFSLCRYTT 360
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ I+QRL
Sbjct: 361 VAEIDQVIAVMPDIIQRL 378
>PI95_22460 cysteine desulfurase
Length = 381
Score = 188 bits (478), Expect = 1e-55, Method: Compositional matrix adjust.
Identities = 122/370 (32%), Positives = 201/370 (54%), Gaps = 7/370 (1%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
M++YLD +ATTP PE ++ + + +GNPSS+H G + +E++R ++A L++
Sbjct: 1 MKIYLDNAATTPLDPEVFEAMKPFMLEDFGNPSSIHSHGRKVRAAIESSRKKIAELLHCT 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW-EITRLG 119
P IIFTSGGTEADN I+ + Y +H I + +EH A++ + LE G E+ +
Sbjct: 61 PGEIIFTSGGTEADNTLIVSGIKTYGL-KHAISTPIEHHAVTHTLETLEKQGQIELHYVK 119
Query: 120 VNHQGRVNPEDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
++ +G V+ DL+ L+ + L+S+++ +EIG + I +G + K +GA FH+D VQ
Sbjct: 120 MDAKGHVDLADLENLLKQYPNALISLMHANNEIGNLLNIKAVGDLAKQYGAFFHSDTVQT 179
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQAT 238
G D++ L V ++ +HK +G G G +Y+R +L + GG QE +R GT+ T
Sbjct: 180 MGHYRHDMRELHVCGMTAGAHKFHGPKGVGFMYIRKDRKLGQFIHGGAQERNMRGGTENT 239
Query: 239 PAIAGFGVAAELAAQELETETGRLILLRDRLFAILA-DVPGLIPTGDKIHRLPHHLSFYL 297
I G A E+A +E++ + L+ R+ L ++PG+ GD L L L
Sbjct: 240 YGIVGLAKALEIAYREMDEHQQYITSLKARMIEKLKQEIPGVEFNGDSA-NLEKSLYTVL 298
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
+ E + L+ L+L GI S G+AC+SG + S +L A+ K G +R +
Sbjct: 299 NVSLPESEENEMLLFNLDLQGISASGGSACSSGASTGSHVLGALYPGSKR--GAVRFSFS 356
Query: 358 KQTTAADIDW 367
K +ID+
Sbjct: 357 KYNKPEEIDF 366
>PI95_34740 cysteine sulfinate desulfinase
Length = 412
Score = 92.8 bits (229), Expect = 2e-21, Method: Compositional matrix adjust.
Identities = 70/234 (29%), Positives = 112/234 (47%), Gaps = 13/234 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-----LHEWGNRAALILETARMQVAGLI 57
VY D +A T +PE + IQ L + + N ++ +H +A E R I
Sbjct: 31 VYFD-NAATNQKPEVV--IQA-LVEYYSNYNANIHRGIHTLAEKATKAYEHTRHLAKEFI 86
Query: 58 NAVPE-SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEI 115
+A E I+F G TEA NL + + +IIS +EH + P + + +
Sbjct: 87 HADSEQEIVFVRGVTEAINLVAATYGKAFIREGDEIIISGLEHHSNIVPWQFVCQERKAL 146
Query: 116 TR-LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ + V G ++ + K+ L T LV+V + + +GT+ PI ++ + + A+ D
Sbjct: 147 LKVIRVKDNGELDIDHFKSLLSDRTKLVAVNHASNSLGTINPIKQIIDLAHANHAVVLID 206
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ 227
Q LP+DV+ L D LSSHK++G G G LY + +ELMP GGG+
Sbjct: 207 GAQAGAHLPIDVKDLNCDFYCLSSHKMFGPTGTGILYGKKSLLELMPPYQGGGE 260
>PI95_11630 cysteine desulfurase
Length = 420
Score = 77.8 bits (190), Expect = 3e-16, Method: Compositional matrix adjust.
Identities = 62/230 (26%), Positives = 104/230 (45%), Gaps = 5/230 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61
+YLD +AT+ + ++ Q N H +A E R +VA +NA
Sbjct: 30 IYLDNAATSQKPLLVLNRLRDYYEQYNANVHRGAHTLSAKATDAYEATRDKVAKFVNAAS 89
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITRL 118
+ I++T +EA NL P +I+S +EH + P +++ + G + +
Sbjct: 90 RQEIVYTRNASEAINLIAYSWGMSNLQPGDEIILSVMEHHSNIVPWQLVAQKTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ + + E K L T LVS ++ + +G + P+ E+ + +GA DA Q
Sbjct: 150 ELTSEETFDLEQFKTLLSEKTKLVSTVHVSNTLGCINPVQEICALAHEYGAKVLIDACQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ 227
+P+DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 210 VPHMPVDVQQIDCDWLVASGHKMCAPTGIGFLYGKFKLLESMPPFFGGGE 259
>PI95_04400 cysteine lyase
Length = 399
Score = 58.2 bits (139), Expect = 8e-10, Method: Compositional matrix adjust.
Identities = 50/168 (29%), Positives = 81/168 (48%), Gaps = 10/168 (5%)
Query: 53 VAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENW 111
+A +N P++I T T N+A+ G+ + HM+++ EH I A+ +
Sbjct: 75 IAQELNVPPQTISLTEDVTVGCNIAMWGLE--WQAGDHMLLTDCEHQGIVAIAQEIGRRL 132
Query: 112 GWEITRLGVN---HQGRVNPEDLKAA-LRHNTVLVSVIYGQSEIGTVQPIAELGKITKTH 167
EIT V ++G +P D A LR NT L+ + + G V P+ ++ ++ K +
Sbjct: 133 KVEITTCPVMATLNEG--DPVDAIAQHLRPNTRLLVLSHILWNTGQVLPLDKIVEVCKKN 190
Query: 168 GALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
DA Q G +PL++ L D + + HK L G G G LY+RP
Sbjct: 191 DVRILVDAAQSVGSMPLNLTELGADFYAFTGHKWLCGPAGVGGLYVRP 238
>PI95_26510 aminotransferase class V
Length = 383
Score = 33.1 bits (74), Expect = 0.069, Method: Compositional matrix adjust.
Identities = 25/82 (30%), Positives = 37/82 (45%)
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+GR E + A+ NT V++ GT + + K T GAL D Q G L
Sbjct: 137 RGRKWNERILDAIDKNTRAVAIPNVHWVDGTRFDLEAIRKRTNDVGALLIIDGTQSVGAL 196
Query: 183 PLDVQTLPVDLLSLSSHKLYGG 204
P D+Q + D L +++K G
Sbjct: 197 PFDLQKVKPDALICAAYKWLMG 218
>PI95_47140 penicillin epimerase
Length = 396
Score = 32.7 bits (73), Expect = 0.080, Method: Compositional matrix adjust.
Identities = 46/217 (21%), Positives = 84/217 (38%), Gaps = 16/217 (7%)
Query: 15 PEAIAIIQTILTQQWGNPS---SLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGT 71
P + Q L +Q N + EW +L+ AR ++AG + A E ++F T
Sbjct: 30 PRTVLKAQQRLREQLENEPLRFFIREWEK----LLDDARSKLAGFVGAPAEDLVFVPNAT 85
Query: 72 EADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRLGVNHQGRVNPED 130
N + + + ++ ++ E++A + G + + + +
Sbjct: 86 TGVNSVLRSLV--FHPEDEILTTNHEYNACRNALDFIASRTGARVVVAKIPFPLESSEQI 143
Query: 131 LKAALRH---NTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
L A + T L + + S+ G + PI +L K + G D G L L++
Sbjct: 144 LSAVIERVSPKTRLALLDHVTSQTGLIFPIQQLVKQLQQRGVDTLVDGAHAPGMLALNLS 203
Query: 188 TLPVDLLSLSSHK-LYGGLGAGALYLRPGV--ELMPL 221
+ + + HK L A LY+RP E+ PL
Sbjct: 204 EIGATYYTGNCHKWLCAPKSAAFLYVRPDKQPEIRPL 240
Database: GCA_000817785.1P
Posted date: Aug 27, 2016 11:11 PM
Number of letters in database: 3,132,640
Number of sequences in database: 10,181
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 10181
Number of Hits to DB: 2,301,901
Number of extensions: 95314
Number of successful extensions: 266
Number of sequences better than 1.0: 10
Number of HSP's gapped: 252
Number of HSP's successfully gapped: 10
Length of query: 382
Length of database: 3,132,640
Length adjustment: 91
Effective length of query: 291
Effective length of database: 2,206,169
Effective search space: 641995179
Effective search space used: 641995179
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)