BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000787675.1P
4790 sequences; 1,244,012 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
N44_04258 cysteine desulfurase 538 0.0
N44_01827 cysteine desulfurase 239 2e-75
N44_02815 cysteine desulfurase 86 3e-19
N44_00355 cysteine desulfurase 60 7e-11
N44_00894 isopenicillin N epimerase 40 2e-04
>N44_04258 cysteine desulfurase
Length = 388
Score = 538 bits (1386), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 260/380 (68%), Positives = 309/380 (81%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR QVAGLINA
Sbjct: 1 MQIYLDYSATTPTHPQVIERVATILRHHWGNPSSLHTWGQDTATVIEMAREQVAGLINAN 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P+ IIFTSGGTEADNLAI+GVA+ Y P+H+IISSVEHSAI+EP K LE GW+ITRL V
Sbjct: 61 PDQIIFTSGGTEADNLAIIGVAQQYNRPRHIIISSVEHSAIAEPCKQLEQQGWQITRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N QGRVNP DLKAA++ +TVL+S+IYGQSE+GT+QPI ELG I + G LFHTDAVQVA
Sbjct: 121 NRQGRVNPLDLKAAIQSDTVLISIIYGQSEVGTLQPIEELGSIARERGVLFHTDAVQVAA 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R +DV+ LPVDLLSLSSHK+YG GAGALY+R GV+++PLL GGGQE GLRSGT A PA
Sbjct: 181 RCDIDVRKLPVDLLSLSSHKIYGMQGAGALYVRAGVDILPLLRGGGQEKGLRSGTPAVPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IA FG+AAELA ++L +E RLI LRDRLF +LAD P L+PTGD+ +RLPHH+SF +
Sbjct: 241 IAAFGLAAELAQKDLISEKMRLIALRDRLFDLLADYPYLLPTGDRFYRLPHHVSFIVRPD 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
D I+GK LVRQLNLAGIGIS+G+AC+SGKLSPSPIL AMGYS + AL GIRLTLG+ T
Sbjct: 301 DDSHITGKQLVRQLNLAGIGISSGSACHSGKLSPSPILKAMGYSDREALAGIRLTLGRDT 360
Query: 361 TAADIDWTGIVVKQILQRLI 380
+AADIDWT +V+KQ++ R +
Sbjct: 361 SAADIDWTALVLKQVIDRCL 380
>N44_01827 cysteine desulfurase
Length = 393
Score = 239 bits (611), Expect = 2e-75, Method: Compositional matrix adjust.
Identities = 147/373 (39%), Positives = 218/373 (58%), Gaps = 13/373 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P+ +A + T+ +GN SS+ H +G A ++ AR +A IN+ P
Sbjct: 6 IYLDCHATTPLDPQVLAAMLPYFTEHFGNASSINHVYGWTAEAAVKQARETIAAAINSSP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLAI GVA Y +H++ EH A+ +P LE G+E+T L V
Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFAKGRHLVTVVTEHRAVLDPCHYLEKLGFEVTILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ E L+ +LR +T+L+S++ +EIG +QP+A +G I + + LFHTDA Q G
Sbjct: 126 GADGLIDLELLEKSLRPDTILLSIMAANNEIGVIQPLAAIGAICRQYQVLFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV + +DL+SL++HK++G G GALY+R P V L + GGGQE GLRSGT
Sbjct: 186 KIPLDVGEMNIDLMSLTAHKVHGPKGVGALYVRRRNPRVRLAAQIQGGGQEKGLRSGTIF 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A EL + +E + L L+ L+ I++ + + G RL +L+ +
Sbjct: 246 TPQIVGFAKAVELGIKAIEEDNSYLNQLKASLWEIISQLDRIYLNGHPSQRLAGNLNISI 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L I +S+G+AC+S +PS +L A+G + A +R L
Sbjct: 306 EGVDGAAL-------LLGLQPIVALSSGSACSSSHTAPSHVLTALGRPESLAYASLRFGL 358
Query: 357 GKQTTAADIDWTG 369
+ T +I+ G
Sbjct: 359 SRFNTLEEIEQVG 371
>N44_02815 cysteine desulfurase
Length = 420
Score = 85.5 bits (210), Expect = 3e-19, Method: Compositional matrix adjust.
Identities = 70/233 (30%), Positives = 114/233 (48%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
+YLD SA T +P I +++T+ + +++H + RA E AR +VA IN
Sbjct: 30 IYLD-SAATSQKP--IQVLETLRNYYEKDNANVHRGAHTLSVRATEAYEGARDKVARFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T TEA NL A +I+S +EH + P +++ + G I
Sbjct: 87 AASRQEIVYTRNATEAINLVAYSWALNTLKAGDEIILSVMEHHSNLIPWQIVCQKTGAVI 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + E K+ L T LV+V++ + +G + P+AE+ + GA DA
Sbjct: 147 KYVQLTSEESFDLEQYKSLLSDKTKLVAVLHVSNTLGCINPVAEIVSLAHQVGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227
Q L +DVQ++ D L S HK+ G G LY + + E MP GGG+
Sbjct: 207 CQSVPHLAIDVQSIDCDWLVASGHKMCAPTGIGFLYGKEAILEAMPPFFGGGE 259
>N44_00355 cysteine desulfurase
Length = 414
Score = 60.1 bits (144), Expect = 7e-11, Method: Compositional matrix adjust.
Identities = 58/216 (26%), Positives = 95/216 (43%), Gaps = 16/216 (7%)
Query: 16 EAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADN 75
EAI L QQ +++W R +L R ++A + P ++ T T N
Sbjct: 58 EAIIDAHNFLQQQGPFSGRVNDWITRKTELL---RQEMAQELGISPSTLSITEDVTVGCN 114
Query: 76 LAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRLGVNHQ-GRVNP-EDLK 132
+A+ GV + +H++++ EH I + + + EI+ + NP E +
Sbjct: 115 IALWGVD--WQAGEHILLTDCEHPGIIATVREIARRYHLEISTCPIRETLNGGNPIEVIS 172
Query: 133 AALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA-----LFHTDAVQVAGRLPLDVQ 187
A LR T ++ V + G V P+ E+ ++ + L DA Q G LPLD+
Sbjct: 173 AHLRPKTRVLVVSHVPWNTGQVLPLKEISQLCHDNSVTEKPVLVVVDAAQSVGCLPLDLS 232
Query: 188 TLPVDLLSLSSHKLYGG-LGAGALYLRPGVELMPLL 222
D + + HK + G G G LY+RP E+ P L
Sbjct: 233 ATGADCYAFTGHKWWCGPAGVGGLYIRP--EIFPSL 266
>N44_00894 isopenicillin N epimerase
Length = 386
Score = 40.0 bits (92), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 39/174 (22%), Positives = 79/174 (45%), Gaps = 7/174 (4%)
Query: 45 ILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
+L+ AR ++A L++ + ++F T A N + + + + ++I+ ++A +
Sbjct: 53 LLDIARQKLADLVSVNSDDLVFVPNATTAVNAVLNSLT--FQENEEILITDQTYNACANA 110
Query: 105 AKML-ENWGWE--ITRLGVNHQGRVN-PEDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
K + + WG + I ++ Q + + + A++ T LV + + S + PIAE+
Sbjct: 111 VKHIAKRWGLKVIIAKIPFPVQSPLEISQAILASVSPRTKLVVLDHVTSPTALIWPIAEI 170
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213
+ G D G LPL++ + + + HK L GA LY+R
Sbjct: 171 VQELNNRGIDTLIDGAHALGFLPLNIVAINPTYYTANCHKWLCSPKGAAFLYVR 224
Database: GCA_000787675.1P
Posted date: Aug 27, 2016 10:33 PM
Number of letters in database: 1,244,012
Number of sequences in database: 4790
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4790
Number of Hits to DB: 941,488
Number of extensions: 39494
Number of successful extensions: 119
Number of sequences better than 1.0: 6
Number of HSP's gapped: 114
Number of HSP's successfully gapped: 6
Length of query: 382
Length of database: 1,244,012
Length adjustment: 84
Effective length of query: 298
Effective length of database: 841,652
Effective search space: 250812296
Effective search space used: 250812296
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)