BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000775285.1P
2731 sequences; 698,048 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DO97_16130 cysteine desulfurase 519 0.0
DO97_18605 cysteine desulfurase 280 2e-91
DO97_10350 cysteine desulfurase 278 3e-90
DO97_16350 polymerase 30 0.10
DO97_17630 CbbY 30 0.13
>DO97_16130 cysteine desulfurase
Length = 403
Score = 519 bits (1336), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 263/390 (67%), Positives = 313/390 (80%), Gaps = 8/390 (2%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLD+SATTPTRPEAIA +Q +L+Q WGNPSSLH+WG R+AL++E AR +VA LINA
Sbjct: 1 MQIYLDHSATTPTRPEAIARMQQVLSQSWGNPSSLHQWGERSALVVEQARQEVASLINAP 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
SI+FT+GGTEA+NLAIMG+A+ Y TPQH+IIS VEH A+SEP + LE GW++TRL V
Sbjct: 61 AASIVFTAGGTEANNLAIMGIAQRYPTPQHVIISQVEHPAVSEPVRWLETLGWQVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ QG VNP +L+AALR TVLVSVIYGQSE+GTVQ IA LG I + HG FHTDAVQVAG
Sbjct: 121 DCQGWVNPLELQAALRPTTVLVSVIYGQSEVGTVQSIASLGSIARAHGVCFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLP+DV+ LPVDLLSLSSHKLYG GAGALY+RPGV+L+P L GGGQE GLRSGTQA A
Sbjct: 181 RLPIDVELLPVDLLSLSSHKLYGPQGAGALYIRPGVQLLPYLRGGGQEQGLRSGTQAVSA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTG--------DKIHRLPHH 292
I+GFGVAA LAA E+ E RL LRDRLFA LAD PGL+PTG RLPHH
Sbjct: 241 ISGFGVAASLAAAEMALEVPRLTALRDRLFAHLADTPGLLPTGVYGATLEEQLRQRLPHH 300
Query: 293 LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGI 352
+S L+ DGE +SG+TLVRQ+NLAGI ISAG+AC+SGK +PSPIL AMGYS + A G I
Sbjct: 301 VSLCLQYPDGEILSGRTLVRQMNLAGIAISAGSACHSGKSTPSPILQAMGYSDRRAQGAI 360
Query: 353 RLTLGKQTTAADIDWTGIVVKQILQRLIPD 382
R TLG+ TTAAD+DW+ +V++QIL RL+P+
Sbjct: 361 RFTLGRDTTAADVDWSAMVLQQILHRLLPE 390
>DO97_18605 cysteine desulfurase
Length = 400
Score = 280 bits (717), Expect = 2e-91, Method: Compositional matrix adjust.
Identities = 155/379 (40%), Positives = 223/379 (58%), Gaps = 19/379 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P + + TQ +GN +S+ H +G A ++ AR +A I A P
Sbjct: 6 IYLDCHATTPVDPRVLEAMLPFFTQYFGNAASVGHTYGWEAEAAIQQARATLAEAIQATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y + +H+I EH+A+ +P + L+ G+E+T L V
Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFSQGRHIITVQTEHNAVLDPCRYLQTLGFEVTYLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G +N DL+ A+R +T+LVSV+ +EIG +QP+A++G ++H LFHTDA Q
Sbjct: 126 QADGLLNLADLEQAMRPDTILVSVMAANNEIGVLQPLADIGACCRSHQVLFHTDAAQAIA 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ + +DL+SL++HK YG G GALY+R P V L P L GGGQE G+RSGT
Sbjct: 186 KIPLDVQAMQIDLMSLTAHKAYGPKGIGALYVRRQHPRVRLAPQLHGGGQERGIRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A L E+ TET RL LR+ L+ L ++P + G + RLP +L+ +
Sbjct: 246 TPQIVGFATAVALGLDEIATETQRLTTLRESLWQTLQELPDIHLNGHPLQRLPGNLNLSV 305
Query: 298 EAADGEKISGKTLVRQLNLAG----IGISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
D + + L G + +S+G+AC + K PS +L A+G S + A +R
Sbjct: 306 AGVDSQAL----------LLGLQPVVALSSGSACTAAKTQPSHVLQAIGRSPELAAASLR 355
Query: 354 LTLGKQTTAADIDWTGIVV 372
+G+ T +ID V
Sbjct: 356 FGIGRFNTPPEIDRVATTV 374
>DO97_10350 cysteine desulfurase
Length = 400
Score = 278 bits (710), Expect = 3e-90, Method: Compositional matrix adjust.
Identities = 159/377 (42%), Positives = 221/377 (58%), Gaps = 6/377 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE +A I LTQ +GNPSS+H +G + L+ AR QVA L+ A
Sbjct: 5 IYLDNNATTQVDPEVVAAILPYLTQYYGNPSSMHSFGGQVGKALKQARNQVASLLGAEES 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
IIFTS GTE +N AI +H+I + VEH+ + K LE G+ +T L VN
Sbjct: 65 EIIFTSCGTEGNNTAIRAAIAAQPDRKHLITTQVEHACVLNLFKQLEKQGYTVTYLSVNR 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G+++ +L+AA+ +T LVS +Y +E GTV P+ + G I K +GA FH DAVQVAG+L
Sbjct: 125 KGQLDLMELEAAITGSTALVSTMYANNETGTVFPVEQAGAIAKEYGATFHVDAVQVAGKL 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T +DL+++S HKL+ G GAL++R G PLL GG QE G R+GT+ I
Sbjct: 185 PLNMKTSTIDLMTISGHKLHAPKGVGALFVRRGFRFRPLLVGGHQERGRRAGTENVAGIV 244
Query: 243 GFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAADG 302
G G AAELA L + LRDRL L + GD+ +RLP+ + + +G
Sbjct: 245 GLGKAAELAQLHL-ADVATEKKLRDRLEKGLLAISDTELNGDRKNRLPNTTNIGFKYIEG 303
Query: 303 EKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTA 362
E I + L+ GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 304 EAI-----LLSLDQYGICASSGSACTSGSLEPSHVLRAMGLPYTILHGSIRFSLSRNTTE 358
Query: 363 ADIDWTGIVVKQILQRL 379
+DID V+ I++RL
Sbjct: 359 SDIDLVLEVMPGIVERL 375
>DO97_16350 polymerase
Length = 473
Score = 30.4 bits (67), Expect = 0.10, Method: Compositional matrix adjust.
Identities = 48/180 (26%), Positives = 73/180 (40%), Gaps = 42/180 (23%)
Query: 25 LTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESII----FTSGG---------- 70
L Q+W S L +WG+ L V GL VP ++I F GG
Sbjct: 30 LLQRWRRSSWLMQWGDELGFCLLA---LVFGLAPFVPNALIGLLLFACGGYWALLTLSDE 86
Query: 71 TEADNLAIMGVARCYTTPQHMII-----SSVEHSAISEPAKMLENWGWE-----ITRLGV 120
TE+ +A R TP H++I + +A+S P KM GW +T +
Sbjct: 87 TESPTIA----NRNGLTPIHLLILLYWGIATVATAVS-PVKMAAFAGWSKLTLYLTAFAL 141
Query: 121 NHQGRVNPE---DLKAALRHNTVLVSV------IYGQSEIGT-VQPIAELGKITKTHGAL 170
+ +P L H +++VSV I+G + + T V P + L K T+ + L
Sbjct: 142 MARILRSPRWRSYLITVYLHISLIVSVYGIRQWIFGANALATWVDPESPLAKTTRVYSFL 201
>DO97_17630 CbbY
Length = 254
Score = 29.6 bits (65), Expect = 0.13, Method: Compositional matrix adjust.
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 1/70 (1%)
Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENG 230
F+ D + P +++ L DL +H L GA+ LRPGV + L Q+
Sbjct: 60 FYLDCYRSDWPRPANLKALIADLHKAKTHYYTQLLATGAIPLRPGVRRL-LEEARSQDMR 118
Query: 231 LRSGTQATPA 240
L T TPA
Sbjct: 119 LAIATTTTPA 128
Database: GCA_000775285.1P
Posted date: Aug 27, 2016 11:09 PM
Number of letters in database: 698,048
Number of sequences in database: 2731
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2731
Number of Hits to DB: 551,910
Number of extensions: 23753
Number of successful extensions: 83
Number of sequences better than 1.0: 5
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 5
Length of query: 382
Length of database: 698,048
Length adjustment: 80
Effective length of query: 302
Effective length of database: 479,568
Effective search space: 144829536
Effective search space used: 144829536
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)