BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000760295.1P
3027 sequences; 695,908 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EV12_1379 Cysteine desulfurase 243 4e-77
EV12_2610 Cysteine desulfurase 234 2e-73
EV12_0704 Cysteine desulfurase 90 5e-21
EV12_2765 Serine--pyruvate aminotransferase 39 2e-04
EV12_2456 hypothetical protein 29 0.18
EV12_0361 Ferredoxin-NADP(+) reductase 29 0.31
>EV12_1379 Cysteine desulfurase
Length = 402
Score = 243 bits (621), Expect = 4e-77, Method: Compositional matrix adjust.
Identities = 143/369 (38%), Positives = 215/369 (58%), Gaps = 14/369 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+ LDY ATTP E + + ++ WGN SS H G +AA + AR Q+A + P
Sbjct: 25 ICLDYQATTPCAAEVVEAMAAYWSEDWGNASSRQHRSGLKAAAAVSLAREQLASHLRVTP 84
Query: 62 ESIIFTSGGTEADNLAIMGVARCYT----TPQHMIISSVEHSAISEPAKMLENWGWEITR 117
+ +IFTSG TEA+N+A++G AR +P H+I EH A+ +P + L+ G+ +T
Sbjct: 85 QRVIFTSGATEANNIALLGHARARAEQRGSPGHLITLITEHHAVLDPLRQLQKEGFRLTE 144
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
L G + PE L A ++T+LVSV+ +E G +QP+A+L ++ + + H+DA Q
Sbjct: 145 LEPRADGLLRPEQLAEAFENDTLLVSVMAANNETGVIQPLAKLAELCRARDVVLHSDAAQ 204
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQA 237
G + LD L +DL+S+S HKLYG G GAL +RP V + PL GGGQE GLR GT
Sbjct: 205 AFGHVHLDPDALALDLISISGHKLYGPKGIGALVVRPEVPINPLQWGGGQEQGLRPGTLP 264
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRLPHHLSFY 296
P I G A ELA +++ + +L LR++L+ L + +P LI G HRLPH+L+
Sbjct: 265 VPLIVGLAKAVELAMKDITSRQDKLCTLRNQLWDGLRERLPDLILNGSLEHRLPHNLNIT 324
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
+ + G +L +QL I S+G+AC+ G +PS +L+A+G + A +RL+L
Sbjct: 325 IPG-----VRGSSLHQQLR-PLIACSSGSACSQG--APSHVLMALGRTSAEAEASLRLSL 376
Query: 357 GKQTTAADI 365
G+ T++ DI
Sbjct: 377 GRNTSSEDI 385
>EV12_2610 Cysteine desulfurase
Length = 399
Score = 234 bits (596), Expect = 2e-73, Method: Compositional matrix adjust.
Identities = 153/368 (41%), Positives = 220/368 (59%), Gaps = 16/368 (4%)
Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVP 61
++YLD SATTP R E I I + WGNPSSLH G AA +LE +R +A + A
Sbjct: 10 EIYLDASATTPPRQEVIRRILEVQESVWGNPSSLHGTGLIAAELLERSRQMIASCLMASA 69
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
++ TSG TE+ +LA++G+AR + + ++IS+VEH A+ A+ L++ GW++ V+
Sbjct: 70 TEVVLTSGATESIHLALLGMARSMQSGR-LVISAVEHPAVLAAAQALKHQGWDVCYWPVD 128
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
GRV EDL+ L T LVS+I+GQSE+GT+QPI +G + G +FHTDA QV +
Sbjct: 129 PLGRVKLEDLELMLAPPTRLVSIIWGQSEVGTIQPIKTIGLACRERGIVFHTDATQVLSQ 188
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPL----LGGGGQENGLRSGTQA 237
L LP+DLLS S+HK G G G L LRP EL+ + GGGGQE GLR+GT++
Sbjct: 189 GLLSWADLPIDLLSASAHKFQGPKGIGLLLLRP--ELIEVLQPLQGGGGQEQGLRAGTES 246
Query: 238 TPAIAGFGVAAE--------LAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRL 289
AG +A E A E+ + ++ +RD L L +PGL TGD I+RL
Sbjct: 247 VALAAGMAIALEQLKLSRPGFADVEM-ADQDKVQRMRDALRINLQTLPGLHFTGDPINRL 305
Query: 290 PHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAAL 349
PHH+S + + D + ISG+ +VR+L+ G+ S+G+AC +G+ S +L AM +
Sbjct: 306 PHHISMLVGSGDDQPISGRAVVRELSRLGVATSSGSACMAGQPKNSDVLQAMQIKPEWLQ 365
Query: 350 GGIRLTLG 357
G+R +LG
Sbjct: 366 SGLRFSLG 373
>EV12_0704 Cysteine desulfurase
Length = 434
Score = 90.1 bits (222), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 102/353 (28%), Positives = 152/353 (43%), Gaps = 37/353 (10%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61
+Y+D++AT+ + + +Q N H+ RA E AR +VA I A
Sbjct: 46 IYMDHAATSQKPRQVLEALQHYYDHDNANVHRGAHQLSVRATEDFERARQKVADFIAASS 105
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKML-ENWGWE 114
I+FT +EA NL VAR + ++++ +EH + P +ML + G
Sbjct: 106 AREIVFTRNASEAINL----VARSWGDANLHEGDEVLLTLMEHHSNIVPWQMLAKRTGCV 161
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ + + G ++ DL+ L T LVS+ + + +G PI+E+ GAL D
Sbjct: 162 LRFVDLTDCGELDLNDLRHKLSERTRLVSLAHLSNVLGCFNPISEVTAEAHRFGALVLLD 221
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG--QENG 230
A Q +P+DV L D L SSHKL G G G L+ R + + P LGGG Q+
Sbjct: 222 ACQSLPHMPVDVSRLGCDFLVGSSHKLCGPTGMGFLWAREELLDAMPPFLGGGEMIQDVY 281
Query: 231 LRSGTQA---------TPAIA---GFGVAAELAAQ----ELETETGRLILLRDRLFAILA 274
L + A TPAI G GVA + Q + +L L +LF L
Sbjct: 282 LDHSSWADLPYKFEAGTPAIGEAIGMGVALDYLKQVGLDRIHAWEQQLTL---QLFDRLQ 338
Query: 275 DVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
D+ GL G + P DG + + L+ AGI I +G C
Sbjct: 339 DIDGLTILGPTPQQEPDRAVLAAFTVDG--LHPNDIGALLDSAGICIRSGHHC 389
>EV12_2765 Serine--pyruvate aminotransferase
Length = 394
Score = 39.3 bits (90), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Query: 130 DLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQT 188
+L+AAL H ++++++ ++ G QP+ +G + + H L D V G +P+ +
Sbjct: 141 ELEAALIEHRPAILALVHAETSTGVCQPMDGVGDLCRKHDCLLLLDTVTSLGAVPVFLDE 200
Query: 189 LPVDL-LSLSSHKLYGGLGAGALYLRPGVE 217
VDL S S L G G + P E
Sbjct: 201 WKVDLSYSCSQKGLSCPPGLGPFTMGPRAE 230
>EV12_2456 hypothetical protein
Length = 256
Score = 29.3 bits (64), Expect = 0.18, Method: Compositional matrix adjust.
Identities = 12/22 (54%), Positives = 16/22 (72%)
Query: 119 GVNHQGRVNPEDLKAALRHNTV 140
G+N QGR P+D+KAAL N +
Sbjct: 93 GINPQGRQTPKDIKAALEVNAL 114
>EV12_0361 Ferredoxin-NADP(+) reductase
Length = 366
Score = 28.9 bits (63), Expect = 0.31, Method: Compositional matrix adjust.
Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 5/57 (8%)
Query: 278 GLIPTGDKIHRLPHHLSFYLEAA--DGEKISGKTL---VRQLNLAGIGISAGAACNS 329
G+IP G + PH L Y A+ G+ + GKT+ VRQL G + C++
Sbjct: 127 GIIPAGTDANGKPHKLRLYSIASTRHGDNLEGKTVSLCVRQLQYELDGKTIDGVCST 183
Database: GCA_000760295.1P
Posted date: Aug 27, 2016 10:39 PM
Number of letters in database: 695,908
Number of sequences in database: 3027
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3027
Number of Hits to DB: 550,683
Number of extensions: 24221
Number of successful extensions: 88
Number of sequences better than 1.0: 6
Number of HSP's gapped: 81
Number of HSP's successfully gapped: 6
Length of query: 382
Length of database: 695,908
Length adjustment: 80
Effective length of query: 302
Effective length of database: 453,748
Effective search space: 137031896
Effective search space used: 137031896
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)