BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000760235.1P
2229 sequences; 518,942 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EV03_0415 Cysteine desulfurase 229 5e-72
EV03_2134 Cysteine desulfurase 219 5e-68
EV03_0525 Cysteine desulfurase 90 5e-21
EV03_1609 Cysteine desulfurase 44 4e-06
EV03_1944 Serine--pyruvate aminotransferase 37 7e-04
EV03_1505 4-alpha-glucanotransferase (amylomaltase) 32 0.041
>EV03_0415 Cysteine desulfurase
Length = 382
Score = 229 bits (584), Expect = 5e-72, Method: Compositional matrix adjust.
Identities = 138/368 (37%), Positives = 208/368 (56%), Gaps = 14/368 (3%)
Query: 5 LDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEW-GNRAALILETARMQVAGLINAVPES 63
D+ ++TP + + + + WGNPS+ + G A+ +E +R ++A +N P+
Sbjct: 8 FDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASYLNINPKR 67
Query: 64 IIFTSGGTEADNLAIMGVARCYT----TPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
+IFTSG TEA+NL ++G AR P H+I S EH A+ +P + L+ G+ +T L
Sbjct: 68 LIFTSGATEANNLGLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQKEGFRLTELQ 127
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
N +G +N E L A +T LVSV+ +EIG +QPI ++G K G FH+DA Q
Sbjct: 128 PNKEGLINIEQLSEAFEKDTFLVSVMAANNEIGVLQPIGDIGSFCKRKGIAFHSDAAQAF 187
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
G L LD +DL+SLS+HK+YG G GAL +R G L P GGGQE GLRSGT P
Sbjct: 188 GYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLRSGTLPVP 247
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAIL-ADVPGLIPTGDKIHRLPHHLSFYLE 298
I GF A E+ + + RL+ R+ L + L ++ GLI G RLPH+L+
Sbjct: 248 LIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPHNLNITFP 307
Query: 299 AADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGK 358
G ++ G+ +R+ I ++G+AC++G+ S +L +G S+K A IR+++G+
Sbjct: 308 GVKGSQLHGQ--LRRF----IFCTSGSACSNGE--ASHVLQEIGLSKKDAEASIRMSIGR 359
Query: 359 QTTAADID 366
TT DI+
Sbjct: 360 NTTEKDIN 367
>EV03_2134 Cysteine desulfurase
Length = 393
Score = 219 bits (558), Expect = 5e-68, Method: Compositional matrix adjust.
Identities = 144/387 (37%), Positives = 216/387 (55%), Gaps = 10/387 (2%)
Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVP 61
+YLD SATTP + I ++ I ++ WGNPSS+H+ G A ILE +R+ +A + A
Sbjct: 3 DIYLDASATTPPHLDVITKLKDIQSECWGNPSSIHKVGVIAREILERSRLSIANKLKASS 62
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
+ + FTSG TE++ LA+ V+ + ++ISSVEH +I+ A L N GW+I V+
Sbjct: 63 DELFFTSGATESNYLALKAVSN-HLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPVD 121
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
G ++ + L+ L T LVS+I+GQSEIG++QPI +G K LFHTDA QV
Sbjct: 122 SYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLIGMECKKRNILFHTDATQVLPC 181
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVE--LMPLLGGGGQENGLRSGTQATP 239
+D L VD+LS S+HKL G G G L LR G++ LM G + +RSGT++ P
Sbjct: 182 GLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESVP 241
Query: 240 AIAGFGVAAELAAQELETETGRLIL-------LRDRLFAILADVPGLIPTGDKIHRLPHH 292
IAGF A +L + +E + + + + RL L + L G I RLP++
Sbjct: 242 LIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHLTFIGPDIERLPNN 301
Query: 293 LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGI 352
LSF I G+ +VR L+ G+ IS+G+AC+S P+PI++A+ + G+
Sbjct: 302 LSFLCHTELMSPIKGREIVRLLSKHGVYISSGSACSSSIQGPNPIMVAINVDKTFQESGL 361
Query: 353 RLTLGKQTTAADIDWTGIVVKQILQRL 379
R+T+G + DI ++ + LQ L
Sbjct: 362 RITIGPWISNDDITSVSNIIFESLQTL 388
>EV03_0525 Cysteine desulfurase
Length = 416
Score = 89.7 bits (221), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 66/229 (28%), Positives = 111/229 (48%), Gaps = 6/229 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
+YLD++AT+ + I ++ + Q N H+ A E +R A IN+
Sbjct: 28 IYLDHAATSQKPKQVIDSLKKYYSFQNANVHRGAHQLSAIATEKFENSRKLTANFINSNN 87
Query: 62 ES-IIFTSGGTEADNL-AIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRL 118
E IIFT TEA NL A +IIS +EH + P +++ + ++ +
Sbjct: 88 EKEIIFTRNATEAINLVAYTWGNYALQENDEIIISLMEHHSNIVPWQLIAKAKKCKLIYI 147
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
++ G ++ +D + L T +VS+++ + +G PI E+ + G+L DA Q
Sbjct: 148 NIDQNGELDFDDFRKKLSDKTKIVSLVHVSNTLGCCNPIEEISSLAHQKGSLVLLDACQS 207
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
+D++ L +D L+ SSHKL G G G L+ R + ++ P LGGG
Sbjct: 208 LAHKQVDIKKLGIDFLAGSSHKLCGPTGIGFLWGREEILKKIPPFLGGG 256
>EV03_1609 Cysteine desulfurase
Length = 391
Score = 43.9 bits (102), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 63/256 (24%), Positives = 103/256 (40%), Gaps = 23/256 (8%)
Query: 2 QVYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
+ Y +Y P +++ AI + T Q P + + W ++ T + +A + +
Sbjct: 7 KAYFNYGGQGPLPTQSLNAITSSWQTIQKLGPFTNNVWPYITKEVITTKNV-IAEICSIH 65
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRLG 119
P+ I FT T L ++G+ ++ ++++S EH I K L + I L
Sbjct: 66 PKRIAFTENVTSGCVLPLLGLP--FSDGDNLLLSDCEHPGIVAACKELAKKKNLTIAILP 123
Query: 120 VNHQGRVNPED----------LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA 169
V+ VN + L+ NT LV + + G + PI + K K H +
Sbjct: 124 VSKLCNVNDNKDETYNTVLKLIDEYLQKNTKLVVLSHLLWNTGQIMPIELISKTLKEHSS 183
Query: 170 --LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKL-YG--GLGAGALYLRPGVELMPLLGG 224
DA Q +P+ D+ + + HK YG GLGA AL R E P L G
Sbjct: 184 KPYLLVDAAQSFCHIPIKGACDTADIYAFTGHKWAYGPEGLGAVALSTRVLEESSPTLIG 243
Query: 225 GGQ---ENGLRSGTQA 237
E G+ +A
Sbjct: 244 WKSLKAEEGIHVNNKA 259
>EV03_1944 Serine--pyruvate aminotransferase
Length = 386
Score = 37.0 bits (84), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 17/73 (23%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
++++ AL+ H ++++++ ++ G QP+ +G + + + L D V G +PL +
Sbjct: 139 QEIEDALKKHTPAVLAIVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLD 198
Query: 188 TLPVDLLSLSSHK 200
+DL S K
Sbjct: 199 EWKIDLAYSCSQK 211
>EV03_1505 4-alpha-glucanotransferase (amylomaltase)
Length = 498
Score = 31.6 bits (70), Expect = 0.041, Method: Compositional matrix adjust.
Identities = 29/122 (23%), Positives = 48/122 (39%), Gaps = 10/122 (8%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTIL-TQQWGNPSSLHEWGNRAALILETARMQVAGLINA 59
+Q D + P PE I I+ T N +SL W E R ++ N
Sbjct: 369 LQFAFDGNLDNPYLPENIKEENWIVYTGTHDNSTSLGWWREMDQDRKEQLRERIFDYEN- 427
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
FTS E + + A+ + P +++ + +++P K NW W +TR
Sbjct: 428 ------FTSW--ELIRIGMETKAKLFVAPMQDLLNLDDSCRLNKPGKTENNWSWRLTRND 479
Query: 120 VN 121
+N
Sbjct: 480 LN 481
Database: GCA_000760235.1P
Posted date: Aug 27, 2016 10:23 PM
Number of letters in database: 518,942
Number of sequences in database: 2229
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2229
Number of Hits to DB: 367,447
Number of extensions: 14799
Number of successful extensions: 59
Number of sequences better than 1.0: 7
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 7
Length of query: 382
Length of database: 518,942
Length adjustment: 78
Effective length of query: 304
Effective length of database: 345,080
Effective search space: 104904320
Effective search space used: 104904320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)