BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000760195.1P
1970 sequences; 508,307 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EV06_0609 Cysteine desulfurase 227 2e-71
EV06_1143 Cysteine desulfurase 218 2e-67
EV06_0717 Cysteine desulfurase 93 4e-22
EV06_1231 Serine--pyruvate aminotransferase 37 4e-04
EV06_0762 Serine--glyoxylate aminotransferase 37 5e-04
EV06_0387 Cysteine desulfurase 28 0.57
>EV06_0609 Cysteine desulfurase
Length = 382
Score = 227 bits (579), Expect = 2e-71, Method: Compositional matrix adjust.
Identities = 139/381 (36%), Positives = 213/381 (55%), Gaps = 14/381 (3%)
Query: 5 LDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN-RAALILETARMQVAGLINAVPES 63
DY +TTP + + + Q WGNPSS + + +A+ + AR Q+A L+ P+
Sbjct: 8 FDYQSTTPCDNKVLEAMFPYWNQDWGNPSSRNNRISIKASAAISLAREQLADLLKISPKR 67
Query: 64 IIFTSGGTEADNLAIMGVARCYTT----PQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
+IFTSG TEA+NLA++G AR P H+I S EH+++ P + L G+ IT L
Sbjct: 68 LIFTSGATEANNLALLGHARAKARQNGFPGHLITLSTEHNSVIAPIRQLRKEGFHITELT 127
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
+ G ++ E LK + +T+LVSV+ +EIG VQPI+E+ + G FH+D Q
Sbjct: 128 PDLDGIISLESLKDSFLEDTLLVSVMIANNEIGVVQPISEIASLCNQRGVAFHSDMSQGF 187
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
G L +D + +DL+S S HK+YG G G L + + PL GG QENGLR+GT P
Sbjct: 188 GNLTIDFSNIGIDLMSFSGHKIYGPKGIGLLVVGEDCIIEPLQWGGDQENGLRAGTLPVP 247
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAIL-ADVPGLIPTGDKIHRLPHHLSFYLE 298
I GF AAE+A Q + +R+ L+ L +P LI G RLP++L+F ++
Sbjct: 248 LIVGFVKAAEIANQNQSFYAKNIKDMRNELWQGLNQKIPDLILNGSLEKRLPNNLNFTVK 307
Query: 299 AADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGK 358
G K L +QL I S+G+AC++G +PS +LL++G S + A +RL+LG+
Sbjct: 308 GVIGSK-----LHKQLR-PFISCSSGSACSNG--APSHVLLSIGRSTQEAEASLRLSLGR 359
Query: 359 QTTAADIDWTGIVVKQILQRL 379
T ++D ++ +++ +L
Sbjct: 360 ATNFDEVDIAINIISKVVYQL 380
>EV06_1143 Cysteine desulfurase
Length = 396
Score = 218 bits (554), Expect = 2e-67, Method: Compositional matrix adjust.
Identities = 138/389 (35%), Positives = 213/389 (54%), Gaps = 16/389 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD SATTP P+ I I + + WGNPSSLH G +A +LE +R+ +A N +
Sbjct: 8 IYLDASATTPPHPDVIQEIYNVQKELWGNPSSLHSVGIKALDLLERSRLSIAKKFNVKSD 67
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
+IFTSG TE+ +LA+ G+A P ++ISSVEH ++ A+ L +GW I V+
Sbjct: 68 QVIFTSGATESIHLALKGIAPN-INPGRIVISSVEHPSVIAAAESLSKYGWIIDYWPVDS 126
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
G ++ L T ++S+I+GQ+E+GT+ PI + K K FHTDA Q+ +
Sbjct: 127 FGSIDLSLTDKFLSAPTKMISLIWGQNEVGTLMPIPLIAKECKKRKIYFHTDATQIISQG 186
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGAL-----YLRPGVELMPLLGGGGQENGLRSGTQA 237
D ++L V+ S S+HK G G G L YL +L+P+ GGG QENG RSGT +
Sbjct: 187 TFDFKSLGVNSFSASAHKFRGPKGIGFLIIDNEYLE---QLIPIQGGGLQENGYRSGTPS 243
Query: 238 TPAIAGFGVAAELAAQELETETGRLI-------LLRDRLFAILADVPGLIPTGDKIHRLP 290
G +A EL + +LI LL +L+ L + L GDK++RLP
Sbjct: 244 VALAHGMAIALELVNVQSTISNNKLIFNDTNTTLLTKKLYESLLKIEHLDFIGDKLNRLP 303
Query: 291 HHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALG 350
+H+SF +++ + IS + LVR+L+ GI +S+G+AC+S + + +L +MG K
Sbjct: 304 NHISFIVKSKTNQPISARRLVRELSEKGIFVSSGSACSSNSIPKNNVLKSMGIDPKLYGS 363
Query: 351 GIRLTLGKQTTAADIDWTGIVVKQILQRL 379
IR++LG D + + + ++ +
Sbjct: 364 SIRISLGNWIENLDTNSLRLTISSTIEHI 392
>EV06_0717 Cysteine desulfurase
Length = 410
Score = 92.8 bits (229), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 91/353 (25%), Positives = 155/353 (43%), Gaps = 37/353 (10%)
Query: 3 VYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA-V 60
+YLD++AT+ + I A++ + H+ +A E AR + I A
Sbjct: 22 IYLDHAATSQKPRQVIEALVHYYSNENANVHRGAHQLSAKATYAFEQAREITSKFIKANS 81
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKML-ENWGWE 114
+ IIFT TEA NL VA + Q ++IS +EH + P ++L + G +
Sbjct: 82 SKEIIFTRNATEAINL----VAYSWGNSQLKEGDEILISLMEHHSNIVPWQLLAKRKGCK 137
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G+ G ++ D + + T +VS+++ + +G+ PI + +I + GA+ D
Sbjct: 138 LRYIGITETGELDINDFRKQVNEKTKIVSILHISNTLGSCNPIKTITEIAHSVGAIVLID 197
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELM-PLLGGGGQENG-- 230
A Q ++V L +D L+ SSHK+ G G G L+ R + E+M P +GGG N
Sbjct: 198 ACQSLAHQAINVNELNIDFLAGSSHKICGPTGCGFLWGREEILEIMPPFIGGGEMINNVS 257
Query: 231 ------------LRSGTQATPAIAGFGVAA-ELAAQELETETGRLILLRDRLFAILADVP 277
+GT A G G A L + LE +L + LF L+++
Sbjct: 258 LYESDWADLPFKFEAGTPAIGEAIGMGAAILYLESIGLENIHSYERMLTEYLFDKLSNLD 317
Query: 278 GLIPTGDKIHRLPHH---LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
++ G + P +FY+ E + + L+ I I +G C
Sbjct: 318 NIVILGPSPKQQPKRAPLATFYI-----ENVHSNDIAELLDSKNICIRSGHHC 365
>EV06_1231 Serine--pyruvate aminotransferase
Length = 379
Score = 37.4 bits (85), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 16/69 (23%), Positives = 35/69 (50%)
Query: 132 KAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPV 191
++ ++H ++++++ ++ G QP+ +G + + + L D V G +PL + V
Sbjct: 135 ESLIKHKPAVLALVHAETSTGVCQPMDGIGDLCRKYNCLLILDTVTSLGSIPLFLDEWKV 194
Query: 192 DLLSLSSHK 200
DL S K
Sbjct: 195 DLAYSCSQK 203
>EV06_0762 Serine--glyoxylate aminotransferase
Length = 383
Score = 37.4 bits (85), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 24/100 (24%), Positives = 48/100 (48%), Gaps = 3/100 (3%)
Query: 106 KMLENWGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSE--IGTVQPIAELGKI 163
K+ + +G + + N +NP+D K+AL ++ + +VI SE G + + E+
Sbjct: 95 KIAKAYGLNVKTIKANWGEPLNPQDFKSALENDNEIRAVIVTHSETSTGVINNLEEISSY 154
Query: 164 TKTH-GALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLY 202
++H A+ D V G + + +D+++ S K Y
Sbjct: 155 VRSHKNAILIADCVTSLGACNVPMDDWGIDVIASGSQKGY 194
>EV06_0387 Cysteine desulfurase
Length = 397
Score = 27.7 bits (60), Expect = 0.57, Method: Compositional matrix adjust.
Identities = 53/226 (23%), Positives = 92/226 (40%), Gaps = 24/226 (10%)
Query: 2 QVYLDYSATTPTRPEAIAIIQT--ILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA 59
+ Y +Y P ++ I + QQ G P + W + I ET ++ +A L
Sbjct: 14 KFYFNYGGQGPLPKASLDSITASWVKIQQLG-PFTNDVWPFISNEIQET-KLLIARLCGV 71
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWG------W 113
+ I T T L ++G+ + + ++IS+ EH + K L +
Sbjct: 72 STKRIALTENVTTGCILPLLGIP--FLEGERILISNCEHPGVVSACKELAKQKKLCIDIF 129
Query: 114 EITRL--GVNHQGRVNPEDLKA---ALRHNT---VLVSVIYGQSEIGTVQPIAE-LGKIT 164
++ L G+ E +K+ +L T VL +++ EI ++ IA LGK
Sbjct: 130 DVQSLSGGIEKAENTTAEVIKSLELSLTPKTRLVVLSHLLWNTGEIMPIELIASFLGKYK 189
Query: 165 KTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKL-YGGLGAGA 209
L DA Q G++P+ D+ + + HK +G G GA
Sbjct: 190 DPPFLL--VDAAQSFGQIPIGSAASKADIYAFTGHKWAFGPEGLGA 233
Database: GCA_000760195.1P
Posted date: Aug 27, 2016 11:15 PM
Number of letters in database: 508,307
Number of sequences in database: 1970
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1970
Number of Hits to DB: 365,375
Number of extensions: 14988
Number of successful extensions: 58
Number of sequences better than 1.0: 8
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 508,307
Length adjustment: 78
Effective length of query: 304
Effective length of database: 354,647
Effective search space: 107812688
Effective search space used: 107812688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)