cyanobase / GCA_001277295.1 - AA650

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000478825.2P 3800 sequences; 1,076,481 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D082_16130 nifS Cysteine desulfurase 486 e-172 D082_15370 nifS Cysteine desulfurase 256 4e-82 D082_10000 nifS Cysteine desulfurase, SufS subfamily 94 5e-22 D082_31320 Cysteine desulfurase 69 7e-14 D082_18500 cefD Cysteine desulfurase 45 3e-06 312490cd41e3ec1f80bdb32aacba00c8

>D082_16130 nifS Cysteine desulfurase Length = 391 Score = 486 bits (1252), Expect = e-172, Method: Compositional matrix adjust. Identities = 242/385 (62%), Positives = 298/385 (77%), Gaps = 3/385 (0%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA- 59 M++YLDYSATTP R E A +Q+ L Q WGNP+SLH WGNRAAL LETAR+QVA L+NA Sbjct: 1 MKIYLDYSATTPPRQEVKAAVQSFLDQSWGNPASLHHWGNRAALALETARLQVAELLNAG 60 Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 P+SIIFTSGGTEA++LA+ GV + Y +PQH+IIS+VEHSAI+ P + LE GW++TRL Sbjct: 61 HPDSIIFTSGGTEANHLALFGVTQNYPSPQHLIISTVEHSAITAPVRWLETQGWQVTRLE 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 VN QGRVNPE+L+ A++ NTVL+S+IYGQSE+GT+QPI ELG+I + FHTDAVQVA Sbjct: 121 VNTQGRVNPEELERAIQSNTVLISIIYGQSEVGTLQPIEELGRIARQRHIPFHTDAVQVA 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 GR P+DV+ LPVDLLSLSSHKLYG GAGALY+ P + + PLL GGGQE GLRSGT P Sbjct: 181 GRFPVDVKHLPVDLLSLSSHKLYGIQGAGALYVHPDLAIGPLLMGGGQEQGLRSGTPPLP 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 AI G GVAA LA QEL E RL LRDR F +LAD L PTGD+++RLPHH+SF +++ Sbjct: 241 AIVGLGVAASLAQQELAEEMTRLQALRDRCFDLLADCEFLAPTGDRLYRLPHHVSFVIQS 300 Query: 300 ADGEK--ISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357 I+GK LVR LN GI ISAGAACNSGKL+PSP+LLAMGY+++ AL G+RL+ G Sbjct: 301 HRPCTVGITGKQLVRALNREGIAISAGAACNSGKLNPSPVLLAMGYAEEQALAGLRLSFG 360 Query: 358 KQTTAADIDWTGIVVKQILQRLIPD 382 ++TT ADI+ T +KQ+ +L P Sbjct: 361 RETTMADIETTARTIKQVFAQLCPQ 385 >D082_15370 nifS Cysteine desulfurase Length = 386 Score = 256 bits (654), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 152/383 (39%), Positives = 215/383 (56%), Gaps = 9/383 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +Y D ATT P + + LT+Q+GN S H +G +A ++ AR +A I A PE Sbjct: 5 LYFDNHATTALDPRVLEAMVPYLTEQYGNAGSAHFYGWQAQGAIKQARQVIADTIGARPE 64 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121 IIFTSG TEA+NLAI GVA Y +H++ EH A+ P + LE G+E+T L V Sbjct: 65 EIIFTSGATEANNLAIRGVAEAYFAEGKHLVTVETEHQAVLAPCRYLETLGFEVTYLPVQ 124 Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181 G VN +L+ ALR +T+LVSV+ +EIG +QP+ E+G++ +FH D Q G+ Sbjct: 125 PNGLVNLTELEKALRPDTILVSVMAANNEIGVIQPLEEIGRLCHRKSIIFHCDGAQALGK 184 Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR--PGVELMPLLGGGGQENGLRSGTQATP 239 +PLDV L +DLLS + HK++G G G LY R PGV L P L GGGQE RSGT P Sbjct: 185 IPLDVGRLNIDLLSFTGHKVHGPKGMGGLYRRQNPGVRLAPQLLGGGQEGNFRSGTLPVP 244 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 I G A +A + L +E R LRD+L+ L + G++ GD RLP +L+ + Sbjct: 245 LIVGLAQALAIAGETLVSEGDRQRQLRDQLWQGLGKIGGVVLNGDYEQRLPGNLNVSITG 304 Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359 D K L+ L + +S+G+AC+S + S +L A+G + AA +R L + Sbjct: 305 VD-----PKALLTSLQ-PRLALSSGSACSSHRTEASHVLYALGRDKTAAQASLRFGLSRF 358 Query: 360 TTAADIDWTGIVVKQILQRLIPD 382 TT A+ID +V + +L D Sbjct: 359 TTEAEIDQAIAIVTNTVAQLRAD 381 >D082_10000 nifS Cysteine desulfurase, SufS subfamily Length = 420 Score = 93.6 bits (231), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 73/233 (31%), Positives = 113/233 (48%), Gaps = 11/233 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58 VY D +AT+ +P+ + ++ +L + +++H + RA E R +VA IN Sbjct: 30 VYFDNAATS-QKPKVV--LEKLLHYYENDNANVHRGAHQLSVRATDAYEGVRSKVAEFIN 86 Query: 59 A-VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKMLENWGWEIT 116 A P I++T TEA N+ A P II++V EH + P +M+ + Sbjct: 87 ARSPREIVYTRNATEAINVVAYSWAMNNLKPGDEIITTVMEHHSNLVPWQMVAAKTGAVL 146 Query: 117 RL-GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 R +N Q + E K+ L T LV+V++ + IG + P+ E+ ++ GA DA Sbjct: 147 RFVQLNEQESFDLEQFKSLLSERTKLVTVVHISNTIGCLNPVEEITRLAHQVGAKVLIDA 206 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227 Q A PLDVQ + D L S HK+ G G LY + + E MP GGG+ Sbjct: 207 CQSAPHYPLDVQVIDCDWLVASGHKMCAPTGIGFLYGKEAILEAMPPFFGGGE 259 >D082_31320 Cysteine desulfurase Length = 434 Score = 69.3 bits (168), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 99/220 (45%), Gaps = 14/220 (6%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNP--SSLHEWGNRAALILETARMQVAGLINAV 60 ++ D +A+T +P+ + T Q + + H RA E AR V +NA Sbjct: 56 IWFD-NASTTQKPQTVIDRLTYFYQHENSNIHRAAHTLAARATDAYENARETVRHFLNAK 114 Query: 61 P-ESIIFTSGGTEADNLAIMGVARCY-----TTPQHMIISSVEHSAISEPAKMLE-NWGW 113 IIF G TE NL VA+ + ++++ +EH A P L G Sbjct: 115 SVNEIIFVKGTTEGINL----VAKSWGKQNIKAGDEILLTHLEHHANIVPWHQLAMETGA 170 Query: 114 EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173 ++ V+ QG++ ++ K L T LV + + + +GTV P E+ + GA Sbjct: 171 KLIVAPVDDQGQILLDEYKKLLGSRTKLVGLCHISNVLGTVTPAQEIVNLAHEIGAKVLI 230 Query: 174 DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR 213 DA Q +P+D+Q L D L S HK++G G G +Y R Sbjct: 231 DAAQSIAHIPIDIQELNPDWLVFSGHKIFGPTGIGVVYGR 270 >D082_18500 cefD Cysteine desulfurase Length = 404 Score = 45.4 bits (106), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 57/236 (24%), Positives = 91/236 (38%), Gaps = 39/236 (16%) Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE 109 R +A N P +I T T ++ + G+ + ++++ EH I + Sbjct: 79 RQALAETFNVDPNTITITDNVTTGCDIVLWGLD--WHQGDEILLTDCEHPGIIAIVQA-- 134 Query: 110 NWGWEITRLGVNHQ-----GRVNPEDLKAALRHN----TVLVSVIYGQSEIGTVQPIAEL 160 R G+ ++ +N D A L ++ T LV + + G V P+AE+ Sbjct: 135 ----IAARFGITYRFFPVAATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEI 190 Query: 161 GKITKTHGALFHT----DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPG 215 + + H + D Q AG LPLD L VD + + HK + G G G LY+ Sbjct: 191 MAVCRRHQGNYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYIHGD 250 Query: 216 V--ELMP--------LLGGGGQENGLRSG-------TQATPAIAGFGVAAELAAQE 254 E+ P G G+ G G T A P AG A +L ++ Sbjct: 251 CLGEINPTYVGWRSITYGAKGEPTGWAEGGKRFEVATSAYPQYAGLLAALQLHQRQ 306 Database: GCA_000478825.2P Posted date: Aug 27, 2016 11:00 PM Number of letters in database: 1,076,481 Number of sequences in database: 3800 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3800 Number of Hits to DB: 848,106 Number of extensions: 37192 Number of successful extensions: 131 Number of sequences better than 1.0: 6 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 1,076,481 Length adjustment: 84 Effective length of query: 298 Effective length of database: 757,281 Effective search space: 225669738 Effective search space used: 225669738 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)