BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000478195.2P
6505 sequences; 1,927,088 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
M595_4442 aminotransferase class-V family protein 580 0.0
M595_1530 nifS cysteine desulfurase NifS 281 4e-91
M595_5857 aminotransferase class-V family protein 276 2e-89
M595_1795 putative cysteine desulfurase 80 4e-17
M595_0516 c-des L-cysteine/cystine lyase C-DES 48 7e-07
M595_5774 aminotransferase class-V family protein 38 0.001
M595_2862 soluble hydrogenase 42 kDa subunit 29 0.83
>M595_4442 aminotransferase class-V family protein
Length = 391
Score = 580 bits (1496), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 285/384 (74%), Positives = 324/384 (84%), Gaps = 3/384 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTR EAIA +Q +LTQQWGN SSLHEWG RAA +LE +R QVA LINA
Sbjct: 1 MQIYLDYSATTPTRREAIAAMQAVLTQQWGNSSSLHEWGQRAATVLERSRSQVAHLINAP 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
PESIIFTSGGTEADN+AIMGVARCY+TPQH+IISSVEHSAI+EPA +LE WGWE+TRL V
Sbjct: 61 PESIIFTSGGTEADNMAIMGVARCYSTPQHLIISSVEHSAIAEPANLLEQWGWEVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ GRV+P DL+AA++ NTVLVSVIYGQSEIGT+QPI LGKI ++ G LFH DAVQVAG
Sbjct: 121 DSLGRVHPLDLQAAIQPNTVLVSVIYGQSEIGTLQPIEALGKIARSQGILFHVDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R+P++V+TLP+DLLSLSSHK YG G GALY +PGVEL PLL GGGQE RSGTQA P
Sbjct: 181 RIPINVETLPIDLLSLSSHKFYGPQGCGALYAKPGVELQPLLTGGGQEMKFRSGTQAIPV 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFG+AAELA +++ TET RLI LRDRLF LADVP LIPTGD+IHRLPHH+SF +
Sbjct: 241 IAGFGMAAELAIKDMVTETPRLIQLRDRLFDGLADVPELIPTGDRIHRLPHHVSFCFQPQ 300
Query: 301 DGEK---ISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
GE I+GKTLVRQ+NLAGIGISAG+AC+SGKLSPSPILLAMGY +K ALGGIRLTLG
Sbjct: 301 TGESSSPITGKTLVRQMNLAGIGISAGSACHSGKLSPSPILLAMGYDEKTALGGIRLTLG 360
Query: 358 KQTTAADIDWTGIVVKQILQRLIP 381
+ T DIDWT V+KQILQRL P
Sbjct: 361 RDTDQVDIDWTAFVLKQILQRLQP 384
>M595_1530 nifS cysteine desulfurase NifS
Length = 400
Score = 281 bits (718), Expect = 4e-91, Method: Compositional matrix adjust.
Identities = 160/379 (42%), Positives = 221/379 (58%), Gaps = 9/379 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P I + LT +GNPSS+H +G + ++ R+Q+A L+ A
Sbjct: 5 IYLDNNATTQVDPAVIEAMLPYLTTYFGNPSSMHSFGGQVGQAVKQGRLQIASLLGAEAS 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I + VEH A+ K LE G+ +T L V+
Sbjct: 65 EIVFTSCGTEGDNAAIRAALSAQPNKRHIITTVVEHPAVLNLCKRLEKEGYTVTYLSVDD 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG ++ +L+AAL NT LVSV+Y +E G + PI E+G+I K GALFH DAVQ G++
Sbjct: 125 QGLLDLHELEAALTGNTALVSVMYANNETGVIFPIEEIGQIVKEKGALFHVDAVQAVGKV 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PLD++T +D+L+LS HK++ G GALY+R GV P + GG QE G R GT+ I
Sbjct: 185 PLDMKTSTIDMLTLSGHKIHAPKGIGALYVRRGVRFRPFMIGGHQERGRRGGTENVAGIV 244
Query: 243 GFGVAAELAAQE-LETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
G AAELA Q LE ET + LRDRL IL+ +P + G RLP+ + +
Sbjct: 245 ALGKAAELAQQHLLEVETEK--RLRDRLEQGILSAIPNTVVNGHSTKRLPNTTNIGFKYI 302
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
+GE I + LN GI S+G+AC SG L PS +L A+G G IR +L + T
Sbjct: 303 EGEAI-----LLSLNQFGICASSGSACTSGSLEPSHVLRALGLPYSVLHGSIRFSLSRYT 357
Query: 361 TAADIDWTGIVVKQILQRL 379
T A+ID V+ Q+++RL
Sbjct: 358 TDAEIDRVLEVMPQVVERL 376
>M595_5857 aminotransferase class-V family protein
Length = 395
Score = 276 bits (706), Expect = 2e-89, Method: Compositional matrix adjust.
Identities = 155/373 (41%), Positives = 227/373 (60%), Gaps = 19/373 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD +ATTP +A + T+ +GNP+S+ H +G A ++ AR +A INA+P
Sbjct: 6 IYLDNNATTPMDERVLAEMIPYFTEHFGNPASINHVYGWEAEAAVKKARETLANAINAIP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKMLENWGWEITRLGV 120
E II TSG TE+DNLA+ GVA Y +IS V EH+A+ +P LEN G+E+T L V
Sbjct: 66 EEIILTSGATESDNLALKGVAEAYFNKGRHLISVVSEHNAVLDPLHYLENLGFELTLLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ L+ A+R +T+LVS++ +EIG +QP+AE+G++ + FHTDA Q G
Sbjct: 126 QSDGLIDLNVLENAIRPDTILVSIMAANNEIGVLQPLAEIGELCRDRNIFFHTDAAQALG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK+YG G GALY+R P V+L P L GGG E G+RSGT
Sbjct: 186 KIPLDVEEMNIDLMSLTAHKIYGPKGIGALYVRRRNPRVKLAPQLHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A +LA +E++TET RL LR +L+ L + + G RL +L+ +
Sbjct: 246 TPQIVGFGEAVKLALKEMKTETERLTKLRQQLWEKLTTLGDIYLNGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI----GISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
DG+ + L G+ +S+G+AC S K+ PS +L A+G S A IR
Sbjct: 306 AGVDGQAL----------LLGLQPVMAVSSGSACTSTKIEPSHVLAALGRSDGLAYASIR 355
Query: 354 LTLGKQTTAADID 366
+G+ T+ +ID
Sbjct: 356 FGIGRFNTSDEID 368
>M595_1795 putative cysteine desulfurase
Length = 420
Score = 79.7 bits (195), Expect = 4e-17, Method: Compositional matrix adjust.
Identities = 67/232 (28%), Positives = 108/232 (46%), Gaps = 12/232 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHE----WGNRAALILETARMQVAGLIN 58
VYLD +A T +P+ + I + G S++H +A E AR +V +N
Sbjct: 30 VYLD-NAATSQKPQVV--IDALRNYYEGYNSNVHRGVHTLSAKATDEYEAARQKVVKFVN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + II+T +EA NL +I+S +EH + P +ML + G +
Sbjct: 87 AASYQEIIYTRNASEAVNLVAYSWGLNNIKAGDEIILSVMEHHSNLVPWQMLAQRTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + E K+ + T LVSV++ + +G + P+ ++ KI +GA DA
Sbjct: 147 KFVELTETQTFDLEQFKSLISTQTKLVSVVHVSNVLGCINPVEDICKIAHENGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
Q +P+DVQ + D L S HK+ G G LY + + + P LGGG
Sbjct: 207 CQSLPHMPVDVQAMDCDWLVGSGHKMCAPTGIGFLYGKLEILNAMSPFLGGG 258
>M595_0516 c-des L-cysteine/cystine lyase C-DES
Length = 397
Score = 48.1 bits (113), Expect = 7e-07, Method: Compositional matrix adjust.
Identities = 48/178 (26%), Positives = 85/178 (47%), Gaps = 13/178 (7%)
Query: 47 ETARMQ--VAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
E+A+M+ +A + E+I T T N+A+ G+ + H+++S EH I
Sbjct: 62 ESAQMRDCMARELGVSAETITLTENVTVGCNIALWGID--WQPGDHLLLSDCEHPGIIAI 119
Query: 105 AKMLENWGWEITRLGVNHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
+ L+ ++I + +N D ++ L+ NT L+ + + G V P+ E+
Sbjct: 120 IQELQR-RFQIEVSVCPLRETLNQGDPVSVIETHLQPNTRLLVISHILWNTGQVLPLGEI 178
Query: 161 GKI--TKTHGAL-FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRP 214
K +++ G + DA Q G LPL++ L VD + + HK + G G+G YL P
Sbjct: 179 VKCCHSQSQGKVRVLVDAAQSVGVLPLNLTALEVDFYAFTGHKWWCGPEGSGGFYLSP 236
>M595_5774 aminotransferase class-V family protein
Length = 393
Score = 37.7 bits (86), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 40/185 (21%), Positives = 76/185 (41%), Gaps = 11/185 (5%)
Query: 35 LHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIIS 94
+ EW + +L+ AR Q+A + + E ++F T N + + ++ ++ +
Sbjct: 56 VREWES----LLDEARRQLAEFLGVIAEELVFVPNATTGINSVLRSLR--FSPTDELLTT 109
Query: 95 SVEHSAISEPAKML-ENWGWEITRLGVNHQGRVNPEDLKAALRH---NTVLVSVIYGQSE 150
+ E++A + E WG + + E + + L +T LV + + S+
Sbjct: 110 NHEYNACRNALNFVAERWGATVKIANIPFPLNSPDEIISSILEQITPHTKLVLIDHISSQ 169
Query: 151 IGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGA 209
G + P+ L + G D G LPL++ + S + HK L GA
Sbjct: 170 TGLIFPLKPLICELNSRGIESLVDGAHAPGMLPLNLPEIGATYYSGNCHKWLSAPKGAAF 229
Query: 210 LYLRP 214
LY+ P
Sbjct: 230 LYVHP 234
>M595_2862 soluble hydrogenase 42 kDa subunit
Length = 385
Score = 28.9 bits (63), Expect = 0.83, Method: Compositional matrix adjust.
Identities = 18/75 (24%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Query: 129 EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHG-ALFHTDAVQVAGRLPLDVQ 187
E L+A + V + + ++ G + + + + K HG AL DAV G + + +
Sbjct: 122 EKLEADTKKEIRAVILTHSETSTGVLNDLETINRYVKAHGEALIMVDAVTSLGAVNIPID 181
Query: 188 TLPVDLLSLSSHKLY 202
+D+++ S K Y
Sbjct: 182 EWGLDVVASGSQKAY 196
Database: GCA_000478195.2P
Posted date: Aug 27, 2016 10:39 PM
Number of letters in database: 1,927,088
Number of sequences in database: 6505
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 6505
Number of Hits to DB: 1,458,628
Number of extensions: 61262
Number of successful extensions: 163
Number of sequences better than 1.0: 9
Number of HSP's gapped: 156
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,927,088
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,354,648
Effective search space: 398266512
Effective search space used: 398266512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)