BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000317695.1P
5838 sequences; 1,879,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Anacy_2017 Cysteine desulfurase 666 0.0
Anacy_2106 cysteine desulfurase NifS 290 3e-94
Anacy_1109 Cysteine desulfurase 276 2e-89
Anacy_1683 cysteine desulfurase 78 2e-16
Anacy_0243 cysteine desulfurase family protein 69 2e-13
Anacy_3373 Cysteine desulfurase 60 1e-10
Anacy_4554 alanine-glyoxylate aminotransferase apoenzyme 39 5e-04
>Anacy_2017 Cysteine desulfurase
Length = 394
Score = 666 bits (1718), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 325/382 (85%), Positives = 354/382 (92%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQVYLDYSATTPTRPEAIA +Q +LTQ+WGNPSSLHEWGNRAAL++ETARMQVAGLINAV
Sbjct: 1 MQVYLDYSATTPTRPEAIAKMQAVLTQEWGNPSSLHEWGNRAALVVETARMQVAGLINAV 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
PESIIFTSGGTEADNLAIMG+ R Y PQH+IISSVEHSA++E +ML+ WGW++T LGV
Sbjct: 61 PESIIFTSGGTEADNLAIMGIVRNYAVPQHIIISSVEHSAVAETVQMLKMWGWKVTHLGV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ GRVNPEDLKAALR NTVLVSVIYGQSE+GTVQPI ELGKI KTHGALFHTDAVQVAG
Sbjct: 121 DSTGRVNPEDLKAALRRNTVLVSVIYGQSEVGTVQPIEELGKIAKTHGALFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLPLDV+TLPVDLLSLSSHKLYG LGAGALY+RPGV + P++GGGGQENGLRSGTQATPA
Sbjct: 181 RLPLDVETLPVDLLSLSSHKLYGPLGAGALYVRPGVNIRPVVGGGGQENGLRSGTQATPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFGVAAELAA+ELETE RLI LRDRLF LADVPGLIPTGDKI+RLPHH+SF LE A
Sbjct: 241 IAGFGVAAELAAKELETERVRLIELRDRLFTNLADVPGLIPTGDKINRLPHHVSFSLEYA 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
DGEK+SGKTLVRQLNLAGIGISAG+AC+SGKLSPSPILLAMGYSQ AALGGIRLTLGKQT
Sbjct: 301 DGEKLSGKTLVRQLNLAGIGISAGSACHSGKLSPSPILLAMGYSQTAALGGIRLTLGKQT 360
Query: 361 TAADIDWTGIVVKQILQRLIPD 382
TAADIDWT +V+KQ+LQRLI D
Sbjct: 361 TAADIDWTAMVLKQVLQRLIQD 382
>Anacy_2106 cysteine desulfurase NifS
Length = 424
Score = 290 bits (741), Expect = 3e-94, Method: Compositional matrix adjust.
Identities = 161/378 (42%), Positives = 224/378 (59%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P + + LT +GNPSS+H +G + ++ AR Q+A L+ A
Sbjct: 9 IYLDNNATTKVDPAVLEAMLPYLTDYYGNPSSMHSFGGQLGKGVKIAREQIAALLGAEES 68
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
IIFTS GTE DN AI G +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 69 EIIFTSCGTEGDNAAIRGALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYTVTYLSVNA 128
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G+++ ++L+A+L NT LVSV+Y +E GT+ PI E+G K +GA+FH DAVQ G++
Sbjct: 129 KGQLDLDELEASLTGNTALVSVMYANNETGTIFPIEEIGARVKEYGAIFHVDAVQAVGKI 188
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T +D+L++S HK++ G G LY+R GV P L GG QE G R+GT+ P I
Sbjct: 189 PLNMKTSTIDMLTISGHKIHAPKGIGVLYVRRGVRFRPFLLGGHQERGRRAGTENVPGII 248
Query: 243 GFGVAAELAAQELETETGRLILLRDRL-FAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAEL LE T R LRDRL A+LA +P GD HRLP+ + + +
Sbjct: 249 ALGKAAELEMLNLEEATKRESRLRDRLEKALLAKIPDCEVNGDPSHRLPNTTNIGFKYIE 308
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 309 GEAI-----LLSLNKYGICASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLCRYTT 363
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ +I++RL
Sbjct: 364 EAEIDQVIAVMPEIVERL 381
>Anacy_1109 Cysteine desulfurase
Length = 389
Score = 276 bits (705), Expect = 2e-89, Method: Compositional matrix adjust.
Identities = 157/382 (41%), Positives = 231/382 (60%), Gaps = 11/382 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP + + T+++GN +S+ H +G + ++ R +A INA P
Sbjct: 6 IYLDNHATTPVDERVLTAMIPYFTEKFGNAASISHIYGWESEAAVKQTRAILATAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI G+A Y QH+I + EH AI +P + L++ G+ IT L V
Sbjct: 66 EEIVFTSGATEANNLAIKGIAEAYFQKGQHIITVATEHKAILDPCEYLKSLGFGITILQV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ DL+ ALR +T+LVSV+ +EIG +QP+A +G+I +FHTDA Q G
Sbjct: 126 QKDGLIDLNDLEKALRPDTILVSVMAANNEIGVLQPLAAIGEICHQRQIIFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK YG G GALY+R P V++ GGG E G+RSGT
Sbjct: 186 KIPLDVEAMNIDLMSLTAHKAYGPKGIGALYVRRRNPRVQIASQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A +E ETE RL+ LR+RL+ L+ V G+ G RL +L+ +
Sbjct: 246 TPQIVGFGKAVEIALEEQETENQRLMELRERLWKHLSTVEGIYLNGHPQKRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
E DG +S ++QL + +S+G+AC+S +PS +L A+G S+K A +R +G
Sbjct: 306 EGVDGAALS--LGLQQL----MAVSSGSACSSANTAPSYVLTALGNSEKLAYASVRFGIG 359
Query: 358 KQTTAADIDWTGIVVKQILQRL 379
+ T +ID V +Q L
Sbjct: 360 RFNTVEEIDTVAQQVISTVQSL 381
>Anacy_1683 cysteine desulfurase
Length = 422
Score = 77.8 bits (190), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 63/231 (27%), Positives = 107/231 (46%), Gaps = 7/231 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNP--SSLHEWGNRAALILETARMQVAGLINAV 60
VYLD +AT+ +P A+ +Q+ + H +A E R ++A INA
Sbjct: 30 VYLDNAATS-QKPLAVLNAWRDYYEQYNSNVHRGAHSLSAKATDAYEATRDKIAKFINAK 88
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITR 117
+ I++T +EA NL P +I+S +EH + P + + + G +
Sbjct: 89 SRQEIVYTRNASEAINLVAYSWGMNNLQPGDEIILSVMEHHSNIVPWQFVAQKTGAVLKF 148
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
+ + + + K + T LVS+++ + +G + P+ E+ I ++GA F DA Q
Sbjct: 149 VELTPEETFDLAQFKQLISQKTKLVSLVHVSNTLGCINPVKEIAAIAHSYGAKFLMDACQ 208
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ 227
+ +DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 209 SVPHMHIDVQEIDCDWLVASGHKMCAPTGIGFLYGKLDLLEAMPPFFGGGE 259
>Anacy_0243 cysteine desulfurase family protein
Length = 460
Score = 68.9 bits (167), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 46/172 (26%), Positives = 82/172 (47%), Gaps = 2/172 (1%)
Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100
R ++ +AR +A L+ + ++F + T + R +I++ ++H+A
Sbjct: 64 RTDALIISARAAIADLLGCDHDEVVFGANMTTLTFAVSRAIGRELQPGDEIIVTCLDHAA 123
Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159
P K LE G I + +N ++ DL++ + T LV+V Y + +GT+ IA+
Sbjct: 124 NISPWKALEERGVTIRTVDINIADCTLDINDLESKINSRTKLVAVSYASNAVGTINDIAK 183
Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALY 211
+ K+ GAL DAV A ++V L D L+ S++K + G LY
Sbjct: 184 IVKLAHAVGALVFVDAVHYAPHASINVHQLDCDFLACSTYKFFAP-HVGVLY 234
>Anacy_3373 Cysteine desulfurase
Length = 385
Score = 60.1 bits (144), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 55/202 (27%), Positives = 91/202 (45%), Gaps = 15/202 (7%)
Query: 46 LETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPA 105
++ + +A L+N +I T T N+ + G+ + H+++S EH + A
Sbjct: 59 MDATKEAIASLLNVPNHTITLTGNVTIGCNIGMWGID--WQAGDHLLLSDCEHPGVIATA 116
Query: 106 KML-ENWGWEITRLGVNHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
K + + E++ + +N D + LR T LV + + G V PI ++
Sbjct: 117 KEISRRFTVEVSTCPL--MATLNEGDPVSIIAQNLRPKTKLVVLSHVLWNTGQVLPIDKI 174
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRPGV--E 217
+I + + +L DA Q G LPL++ L VD + + HK L G G G LY+R
Sbjct: 175 TEICRNNNSLLLVDAAQSVGVLPLNLTELGVDFYAFTGHKWLCGPAGVGGLYVREEARER 234
Query: 218 LMPLLGGGGQENGLRSGTQATP 239
L P G NG+ + QA P
Sbjct: 235 LQPTFIG---LNGVITNNQAQP 253
>Anacy_4554 alanine-glyoxylate aminotransferase apoenzyme
Length = 382
Score = 39.3 bits (90), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 22/82 (26%), Positives = 43/82 (52%), Gaps = 4/82 (4%)
Query: 127 NPEDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLD 185
N +++ A+ H ++++++ ++ G QP+ E+G++ + +G L D V G +P+
Sbjct: 135 NLDEISTAVETHKPAILALVHAETSTGARQPLEEVGELCRKYGTLLLVDTVTSLGGVPIF 194
Query: 186 VQTLPVDLLSLSSHKLYGGLGA 207
+ VDL S K GLG
Sbjct: 195 LDEWGVDLAYSCSQK---GLGC 213
Database: GCA_000317695.1P
Posted date: Aug 27, 2016 11:16 PM
Number of letters in database: 1,879,690
Number of sequences in database: 5838
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5838
Number of Hits to DB: 1,407,686
Number of extensions: 59273
Number of successful extensions: 182
Number of sequences better than 1.0: 9
Number of HSP's gapped: 175
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,879,690
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,365,946
Effective search space: 401588124
Effective search space used: 401588124
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)