BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000317655.1P
2837 sequences; 903,685 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Cyast_0200 Cysteine desulfurase 463 e-163
Cyast_1608 Cysteine desulfurase 266 6e-86
Cyast_2591 Cysteine desulfurase 221 7e-69
Cyast_1959 cysteine desulfurase 81 7e-18
Cyast_1536 aminotransferase class V 44 1e-05
Cyast_0579 aminotransferase class V 39 2e-04
Cyast_2767 protein of unknown function DUF490 30 0.25
>Cyast_0200 Cysteine desulfurase
Length = 386
Score = 463 bits (1192), Expect = e-163, Method: Compositional matrix adjust.
Identities = 240/383 (62%), Positives = 290/383 (75%), Gaps = 2/383 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA- 59
MQ+YLD SATT R E I ++Q L + WGNPSSLH WG RA LILE AR QVA LINA
Sbjct: 1 MQIYLDASATTAPRVEVINLMQETLLKGWGNPSSLHSWGERATLILEKARWQVASLINAP 60
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
++IIFTSGGTEADNLA+ GV Y +PQH+IIS+VEHSAI+ PA++LEN GW++TRL
Sbjct: 61 SSDAIIFTSGGTEADNLALFGVTTQYDSPQHIIISAVEHSAIALPAQILENQGWQVTRLA 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ +GRVNP+ L A++ NTVLVS+IYGQSE+GT+QPI EL ITK G LFHTDAVQVA
Sbjct: 121 VDSRGRVNPQALSMAIQPNTVLVSIIYGQSEVGTIQPIKELATITKNQGILFHTDAVQVA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRL LDV+ L +DLLSLS+HK YG GAGALY+RPGV+L L GGGGQE GLRSGTQA
Sbjct: 181 GRLTLDVEELGIDLLSLSAHKFYGIQGAGALYIRPGVKLNSLGGGGGQEKGLRSGTQALG 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
AIA G+A++L +ELE ET ++ LRD L +L+ P L TGD IHRLPHH SF +
Sbjct: 241 AIASMGLASQLIQEELELETPKIRALRDYLLELLSSFPYLTITGDTIHRLPHHASFIINH 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
++G+ LVR+LN AGIGISAG+ACNSGK PS LLAMGY+Q AL GIR++L +
Sbjct: 301 PS-PLLTGRKLVRELNFAGIGISAGSACNSGKTQPSSTLLAMGYTQNQALKGIRISLDRH 359
Query: 360 TTAADIDWTGIVVKQILQRLIPD 382
TT DI WT +V++QI+ RLI D
Sbjct: 360 TTKEDITWTAMVLRQIINRLISD 382
>Cyast_1608 Cysteine desulfurase
Length = 383
Score = 266 bits (680), Expect = 6e-86, Method: Compositional matrix adjust.
Identities = 147/369 (39%), Positives = 221/369 (59%), Gaps = 11/369 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP + + T +GN SS+ H +G A ++ AR +A INA
Sbjct: 4 IYLDCHATTPMDKRVLEAMMPFFTDYFGNASSVAHIYGWEAEAGIKKAREIIARAINAKE 63
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
I+FTSG TEADNLAI GVA Y +H+I + EH A+ EP + L + G+E++ L V
Sbjct: 64 NEIVFTSGATEADNLAIKGVAEAYFDKGKHIITVATEHKAVIEPCEYLMDLGFEVSFLPV 123
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N G ++ + L+ +R +T+LVSV+ +EIG +QP+A++G+I G +FHTDA Q G
Sbjct: 124 NKNGLLDLDLLRQTIRDDTILVSVMAANNEIGVLQPLADIGQICHDRGVIFHTDAAQAIG 183
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ + +DL+SL++HK+YG G GALY+R P V++ + GGGQEN +RSGT
Sbjct: 184 KIPLDVQAMNIDLMSLTAHKIYGPKGIGALYIRRRNPRVKIASQIQGGGQENKIRSGTLC 243
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A +E E + +RD L+ ++ + G+I GD RL +L+ +
Sbjct: 244 TPNIVGFGSAVEIAMNSMEKENQKQKKMRDYLWENISQLDGIILNGDLEKRLSGNLNISV 303
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
E DG + L Q +A +S+G+AC S PS +L A+G+ + A +R +G
Sbjct: 304 EGVDGAAL---LLALQPTVA---VSSGSACTSESTKPSHVLTALGHPKSLAQASLRFGIG 357
Query: 358 KQTTAADID 366
+ T +++
Sbjct: 358 RFNTDEEME 366
>Cyast_2591 Cysteine desulfurase
Length = 378
Score = 221 bits (564), Expect = 7e-69, Method: Compositional matrix adjust.
Identities = 134/381 (35%), Positives = 207/381 (54%), Gaps = 13/381 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY +TTP I +I + +GNPSS+ H WG+R +++ A+ Q+A LIN
Sbjct: 5 IYLDYHSTTPVDRRLIDVIYDSMIDNFGNPSSIDHLWGDRTLNLIKKAKQQIANLINCQA 64
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW-EITRLGV 120
+ IIFTSG TE+ N I G+ T P H IIS +EH A+ + + + G +I L V
Sbjct: 65 QEIIFTSGATESINTVIQGL----TPPNHFIISPLEHKAVIDTCQAMVKRGLGKIRWLKV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ +GR++ E L+ L+ V+ +EIG + PI E+GKI +T+ F D Q G
Sbjct: 121 DKKGRIDLEYLEKMCLQGASLLCVMAANNEIGNIYPIQEIGKIAQTYNIPFLCDGSQAVG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
++PL+ + + L++S HKLY G GAL ++ G L PL+ GGG +NGLRSGT
Sbjct: 181 KIPLNFEDWGITFLTISGHKLYAPKGIGALIIKKGFTLQPLIYGGGHQNGLRSGTLNVSG 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAIL-ADVPGLIPTGDKIHRLPHHLSFYLEA 299
I G A L E+ + + L R+ L +IL A +P LI GD +RL +L +
Sbjct: 241 IVALGEACYLRQLEMVEDEKAIALKRNHLQSILQAQIPSLIVNGDIDNRLAGNLHISIPD 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
I + VR + IS G+AC + L+PS +L A+ + G +R+ +GK
Sbjct: 301 VPNSAIIAR--VRD----KLAISTGSACTTETLAPSHVLRAINLEDELIEGALRIGIGKF 354
Query: 360 TTAADIDWTGIVVKQILQRLI 380
TT +I+ + ++ I+ +++
Sbjct: 355 TTHEEIEKSADILINIINQVL 375
>Cyast_1959 cysteine desulfurase
Length = 419
Score = 81.3 bits (199), Expect = 7e-18, Method: Compositional matrix adjust.
Identities = 68/232 (29%), Positives = 109/232 (46%), Gaps = 12/232 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
+Y D +AT+ +P A+I + N +++H + RA E AR +VA IN
Sbjct: 30 IYFDNAATS-QKP--TAVINALKNYYEYNNANVHRGAHSLSGRATDDYEGARDKVAKFIN 86
Query: 59 AVPES-IIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKML-ENWGWEI 115
A + I++T +EA N+ P II SV EH + P +++ + G +I
Sbjct: 87 ARSRNEIVYTRNASEAINIVAYTWGLSNLQPDDEIILSVMEHHSNIVPWQIIAQKTGAKI 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + K L T LVS+++ + +G + P+ E+ + GA DA
Sbjct: 147 RFVELTDTEEFDLQQYKTFLNEKTKLVSIVHVSNTLGCINPVEEIINLAHKQGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG--VELMPLLGGG 225
Q LP+DVQ + D L S HK+ G G LY + +E+ P LGGG
Sbjct: 207 CQSLPHLPIDVQAMDCDWLVGSGHKMCATTGIGFLYGKEELLLEMPPFLGGG 258
>Cyast_1536 aminotransferase class V
Length = 383
Score = 43.5 bits (101), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 45/187 (24%), Positives = 85/187 (45%), Gaps = 13/187 (6%)
Query: 35 LHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIIS 94
++ W N ++ + +A N+ E+I + T + N+A+ G+ + +++S
Sbjct: 52 INSWVNEN---IDNVKSAIALETNSKIETITLSENVTGSCNIALWGIE--WKAGDEILLS 106
Query: 95 SVEH-SAISEPAKMLENWGWEITRLG-VNHQGRVNP-EDLKAALRHNTVLVSVIYGQSEI 151
EH I+ ++ +G +++ V+ + NP E +K L T LV + +
Sbjct: 107 DAEHPGVIASIKEISRRFGVKVSTFALVDTLNQGNPVEVIKNHLTPATRLVVISHVLWNT 166
Query: 152 GTVQPIAELGKITKTHGAL----FHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLG 206
G V P+ E+ ++ + + D Q AG LPL++ VD + HK L G G
Sbjct: 167 GQVLPLKEISQVCHQYPSKKPIQILVDGAQSAGLLPLNLPDTGVDFYGCTGHKWLCGPTG 226
Query: 207 AGALYLR 213
G LY++
Sbjct: 227 VGFLYVK 233
>Cyast_0579 aminotransferase class V
Length = 364
Score = 38.9 bits (89), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 28/103 (27%), Positives = 48/103 (46%), Gaps = 5/103 (4%)
Query: 105 AKMLENWGWEITRLGVNHQGRVNPEDLKAALRHNT----VLVSVIYGQSEIGTVQPIAEL 160
AKM +G E+ + ++PE +A L +T V + + ++ G + + +
Sbjct: 94 AKMSRKFGLEVEEISAEWGHPLDPEAFRAKLEADTEKKIKAVIITHSETSTGVLNDLETI 153
Query: 161 GKITKTHG-ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLY 202
K K HG AL DAV G + L + L +D+++ S K Y
Sbjct: 154 NKHVKAHGEALIIVDAVTSLGAVDLKIDELGLDVVASGSQKGY 196
>Cyast_2767 protein of unknown function DUF490
Length = 1848
Score = 30.0 bits (66), Expect = 0.25, Method: Composition-based stats.
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 8/61 (13%)
Query: 171 FHTDAVQVAGRLPL-------DVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLG 223
F + V G LPL D+ T +D L+LS LY G G G + + G + P++G
Sbjct: 1481 FSEGEITVTGGLPLFIRDSQGDMLTASIDNLALSLPNLYEGSGRGNIEI-GGSAIAPMIG 1539
Query: 224 G 224
G
Sbjct: 1540 G 1540
Database: GCA_000317655.1P
Posted date: Aug 27, 2016 11:04 PM
Number of letters in database: 903,685
Number of sequences in database: 2837
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2837
Number of Hits to DB: 666,060
Number of extensions: 27848
Number of successful extensions: 85
Number of sequences better than 1.0: 7
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 7
Length of query: 382
Length of database: 903,685
Length adjustment: 83
Effective length of query: 299
Effective length of database: 668,214
Effective search space: 199795986
Effective search space used: 199795986
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)