cyanobase / GCA_001277295.1 - AA650

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000317615.1P 3337 sequences; 1,011,999 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Dacsa_3448 cysteine desulfurase family protein 520 0.0 Dacsa_2821 cysteine desulfurase family protein 275 1e-89 Dacsa_1279 cysteine desulfurase-like protein, SufS subfamily 98 1e-23 Dacsa_1897 selenocysteine lyase 66 5e-13 Bdb6e7aa2233f1ebd0b5bfcb0e1d79ff

>Dacsa_3448 cysteine desulfurase family protein Length = 389 Score = 520 bits (1338), Expect = 0.0, Method: Compositional matrix adjust. Identities = 256/381 (67%), Positives = 309/381 (81%), Gaps = 2/381 (0%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA- 59 MQ+YLD+SATTP P+ I +QT+L+QQWGNPSSLH WGNRAA +E AR +VA LINA Sbjct: 1 MQIYLDHSATTPPHPDVITKVQTVLSQQWGNPSSLHSWGNRAATEIEIARGEVAQLINAA 60 Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 PE+I+FTSGGTE+DNLA++GV Y++PQH+IISSVEHSAI + AK LE GWE+TRL Sbjct: 61 TPETILFTSGGTESDNLALLGVVAQYSSPQHLIISSVEHSAIEQTAKRLETRGWEVTRLP 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 V+ +GR++P DL+ A++ NTVLVS+IYGQSE+GT+QPIAEL +I G LFHTDAVQV Sbjct: 121 VDTKGRIHPWDLETAIQSNTVLVSIIYGQSEVGTLQPIAELSRIAHQKGVLFHTDAVQVT 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 G+LP+DVQ LPVD LSLS HK YG GAGALY+R G++L PLL GGGQE LRSGTQA Sbjct: 181 GKLPIDVQQLPVDFLSLSGHKFYGMQGAGALYVRSGIDLKPLLTGGGQERQLRSGTQAVG 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 AIAG GVAA+LA E+ TET RL LR++LF +LAD P LIPTGD+ RLPHH+SF ++ Sbjct: 241 AIAGLGVAAQLAKAEMTTETPRLEKLREQLFDLLADCPYLIPTGDRTQRLPHHVSFCVKT 300 Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359 + E I+GKTLVR+LNLAGIGISAG+AC+SGKL PSPIL AMGYS+ AL GIRLTLG+ Sbjct: 301 PE-ETITGKTLVRELNLAGIGISAGSACDSGKLKPSPILSAMGYSETMALAGIRLTLGRS 359 Query: 360 TTAADIDWTGIVVKQILQRLI 380 TT ADI WT +V+KQ+LQRLI Sbjct: 360 TTEADIKWTAMVLKQVLQRLI 380 >Dacsa_2821 cysteine desulfurase family protein Length = 385 Score = 275 bits (704), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 162/369 (43%), Positives = 228/369 (61%), Gaps = 11/369 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLD ATTP P + + T+ +GNPSS+ H +G A ++ AR +A INA P Sbjct: 5 IYLDCHATTPVDPRVLDAMMPYFTEHFGNPSSVSHAYGWEAEAGVKQAREIIAEAINASP 64 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E I+FTSG TEA+NLAI GVA Y + QH+I + EH A+ +P K LE+ G+EIT L V Sbjct: 65 EEIVFTSGATEANNLAIKGVAEAYFSQGQHIITVATEHRAVIDPCKYLESLGFEITFLTV 124 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 G ++ + L+ ++R +T+LVSV+ +EIG +QP+ E+G + + G LFHTDA Q G Sbjct: 125 GEDGLIDLDILEKSIRSDTILVSVMAANNEIGVLQPLKEIGALCREKGVLFHTDAAQAIG 184 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237 ++PLDV+ + ++L+SL++HKLYG G GALY+R P V L + GGGQE LRSGT Sbjct: 185 KIPLDVEAMNINLMSLTAHKLYGPKGIGALYVRRRNPKVYLKSQIQGGGQERELRSGTLY 244 Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297 P I GFG A L E+ETE RL LRDR L + G+ GD+ RLP++L+ + Sbjct: 245 PPQIVGFGKAVALGLAEMETEGERLEKLRDRAMEKLLQLEGVYLNGDRAQRLPNNLNISV 304 Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357 D G L+ L I +S+G+AC+S ++PS ++ A+G S+ A IR +G Sbjct: 305 AGVD-----GTALILGLQPV-IAVSSGSACSSQSVAPSHVITALGRSKTLANASIRFGMG 358 Query: 358 KQTTAADID 366 + TTA DID Sbjct: 359 RFTTAEDID 367 >Dacsa_1279 cysteine desulfurase-like protein, SufS subfamily Length = 420 Score = 98.2 bits (243), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 111/402 (27%), Positives = 168/402 (41%), Gaps = 45/402 (11%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61 +Y D +AT+ I ++ Q N H +A E AR +VA INA Sbjct: 30 IYFDNAATSQKPLSVINALEDYYQQVNSNVHRGAHSLSTKATEKFEGARDKVAQFINATS 89 Query: 62 ES-IIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKML-ENWGWEITRL 118 + I+FT TEA NL A + P I++SV EH + P +M+ + G I + Sbjct: 90 RNEIVFTRNATEAVNLVAYSWAINHLKPGDEILTSVMEHHSNLVPWQMVAQKTGAIIKHV 149 Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178 G+ + ++ E K+ L T LV++++ + +G + P+AE+ K+ GA DA Q Sbjct: 150 GLTAEQTLDLEQYKSLLSEKTKLVALVHVSNTLGCINPVAEITKLAHEKGAKVLIDACQS 209 Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ---ENGLRSG 234 LP+DVQ + D L S HK+ G G LY + +E MP GGG+ E L S Sbjct: 210 VPHLPVDVQAINCDWLVASGHKMCAPTGIGFLYGKEDLLEAMPPFLGGGEMIGEVSLDSF 269 Query: 235 TQA---------TPAIA---GFGVAAELAA-------QELETETGRLILLRDRLFAILAD 275 T A TPAI G A + + E E + + F L + Sbjct: 270 TYAELPHKFEAGTPAIGEAIALGAAVDYLSNIGMAKIHAYEEELTAYLFQQLEPFENL-N 328 Query: 276 VPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPS 335 + G PT D R L + E + L L+ G+ I +G C Sbjct: 329 LYGPKPTSDGKGRAA------LATFNVENLHANDLAPLLDYEGVAIRSGHHCT------Q 376 Query: 336 PILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQILQ 377 P+ +G + G R +L T +ID I +K ++ Sbjct: 377 PLHTVLGVA-----GSARASLYFYNTREEIDRFVIALKSTIE 413 >Dacsa_1897 selenocysteine lyase Length = 376 Score = 66.2 bits (160), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 65/236 (27%), Positives = 102/236 (43%), Gaps = 26/236 (11%) Query: 4 YLDYSATTPTRPEAIAIIQTILTQ-QWGNPSSLHEWGNRAALILETA--RMQVAGLINAV 60 Y ++ P P+ ++ I+ + Q P S G A L E+A R +A + Sbjct: 8 YFNFGGQGPLSPQTLSAIEQGYDKIQEQGPFSQ---GVNAWLQEESANTRQAIAQELGTT 64 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 PE+I T T N+A+ G++ + H+++S EH I + + + R G+ Sbjct: 65 PETITLTENVTVGCNIALWGLS--WQKQDHILLSDCEHPGIIATIQEIAD------RFGI 116 Query: 121 NHQ-----GRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALF 171 N+ +N D K LR NT LV + + G V P+ + ++ G Sbjct: 117 NYSTCPIMDTLNQGDPTAVFKQHLRENTKLVVLSHLLWNTGQVLPLKAITEVCHQQGVKV 176 Query: 172 HTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYL-RPGV-ELMPLLGG 224 DA Q G LPLD+ VD + + HK L G G G LY+ +P +L P G Sbjct: 177 LVDAAQSVGSLPLDLPHTDVDFYAFTGHKWLCGPAGVGGLYISKPSFSDLRPTFIG 232 Database: GCA_000317615.1P Posted date: Aug 27, 2016 10:56 PM Number of letters in database: 1,011,999 Number of sequences in database: 3337 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3337 Number of Hits to DB: 770,373 Number of extensions: 33009 Number of successful extensions: 121 Number of sequences better than 1.0: 5 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 5 Length of query: 382 Length of database: 1,011,999 Length adjustment: 83 Effective length of query: 299 Effective length of database: 735,028 Effective search space: 219773372 Effective search space used: 219773372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)