BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000317145.1P
5945 sequences; 1,793,467 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Cha6605_5178 cysteine desulfurase family protein 520 0.0
Cha6605_3791 cysteine desulfurase family protein 287 1e-93
Cha6605_0229 cysteine desulfurase-like protein, SufS subfamily 81 2e-17
Cha6605_2881 cysteine desulfurase family protein, VC1184 subfamily 58 4e-10
Cha6605_3714 selenocysteine lyase 45 8e-06
Cha6605_5618 serine-pyruvate aminotransferase/archaeal aspartate... 39 8e-04
Cha6605_4341 selenocysteine lyase 35 0.010
Cha6605_4439 serine-pyruvate aminotransferase/archaeal aspartate... 29 0.67
>Cha6605_5178 cysteine desulfurase family protein
Length = 389
Score = 520 bits (1340), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 255/384 (66%), Positives = 305/384 (79%), Gaps = 3/384 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLD+SATTPTR EAIA + ++T +WGNPSS+H+WG RA+ +E AR QVA LINA
Sbjct: 1 MQIYLDHSATTPTRTEAIAAMHRVITSEWGNPSSIHQWGERASTAIEQARFQVAALINAN 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P++I F SGGTEA+N AIMGV Y TPQH+IISSVEHS+IS P + LE+ GW++TRL V
Sbjct: 61 PDAIAFGSGGTEANNQAIMGVVNRYDTPQHLIISSVEHSSISAPVQWLESKGWQVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ GR++P DL A+R NT+LVS+IY QSE+GT+QPIAEL I + H FHTDAVQ AG
Sbjct: 121 DRSGRIDPRDLAVAIRPNTILVSIIYAQSEVGTIQPIAELAAICRQHQVCFHTDAVQAAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
++ LDVQ L +DLLSLSSHKLYG GAGALY+RPG+ L+PLL GGGQE G RSGTQA P
Sbjct: 181 KIALDVQKLGIDLLSLSSHKLYGPQGAGALYIRPGLALVPLLAGGGQEMGWRSGTQAVPQ 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFGVAAEL ++EL E RLI LRDRLF +LAD P L+PTGD HRLPHHLSF L+
Sbjct: 241 IAGFGVAAELVSEELAIEQHRLIGLRDRLFELLADCPLLVPTGDLQHRLPHHLSFCLQIP 300
Query: 301 DG---EKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
E ++GKT+VRQLNLAGIGIS+GAAC SGKL+PSPILLAMGY+Q ++GGIRLTLG
Sbjct: 301 RCNFQEPVTGKTIVRQLNLAGIGISSGAACASGKLTPSPILLAMGYTQTESVGGIRLTLG 360
Query: 358 KQTTAADIDWTGIVVKQILQRLIP 381
+ TT ADI+WT V+KQIL RL P
Sbjct: 361 RSTTLADIEWTAFVLKQILDRLTP 384
>Cha6605_3791 cysteine desulfurase family protein
Length = 391
Score = 287 bits (735), Expect = 1e-93, Method: Compositional matrix adjust.
Identities = 160/388 (41%), Positives = 228/388 (58%), Gaps = 19/388 (4%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINA 59
+ +YLD ATTP P +A + T+ +GNP+S HE+G A + AR +A I A
Sbjct: 7 LPIYLDRHATTPVDPRVLAAMLPYFTEHFGNPTSTTHEYGWTAEAAVTGARETIATAIGA 66
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRL 118
PE I+FTSG TEA+NLA+ GVA Y QH+I EH+A+ +P K LE+ G+ +T L
Sbjct: 67 SPEEIVFTSGATEANNLALKGVAEAYFAKGQHIITLQTEHNAVLDPCKYLESLGFTVTYL 126
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
V G V+ ++LK A R +T+LVS++ +EIG +QP+ ++GK+ + +G LFHTDA Q
Sbjct: 127 PVQSDGLVDLDELKNAFRPDTILVSIMSANNEIGVLQPLVDIGKLCRENGVLFHTDAAQS 186
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGT 235
G++PLDV+ L +DL+S+++HK+YG G GALY+R P V+L P L GGG E G+RSGT
Sbjct: 187 IGQIPLDVELLNIDLMSMTAHKVYGPKGIGALYVRRRNPRVKLSPQLHGGGHERGMRSGT 246
Query: 236 QATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSF 295
P I GF A E E+E ET RL LR RL++ L+ + G+ G RLP +L+
Sbjct: 247 LYPPQIVGFAKAVEFGIAEMEVETARLTQLRARLWSQLSQIDGIYLNGHPTLRLPGNLNI 306
Query: 296 YLEAADGEKISGKTLVRQLNLAG----IGISAGAACNSGKLSPSPILLAMGYSQKAALGG 351
+ DG + L G + +S+GAAC+S K+ PS +L A+G S+ A
Sbjct: 307 SIAGVDGNAL----------LLGLRPTVALSSGAACSSAKIEPSHVLRAIGRSESLAYAA 356
Query: 352 IRLTLGKQTTAADIDWTGIVVKQILQRL 379
+R +G T ID +Q L
Sbjct: 357 LRFGIGHNNTVEQIDRVATATIATVQSL 384
>Cha6605_0229 cysteine desulfurase-like protein, SufS subfamily
Length = 444
Score = 80.9 bits (198), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 96/347 (27%), Positives = 149/347 (42%), Gaps = 27/347 (7%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ I ++ + N +H + A E AR ++A +NA
Sbjct: 57 VYLDNAATSQKPHAVIHALEHYYSHYNSNVHRGVHTLSSEATDAYEGAREKIAKFVNAAS 116
Query: 62 -ESIIFTSGGTEADNLAIM--GVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITR 117
+ II+T TEA NL G++ + +I+S +EH + P +++ + G +
Sbjct: 117 SQEIIYTRNATEAINLVAYSWGLSNLQRGDE-VILSVMEHHSNLIPWQLVAQKTGARLQF 175
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
+ + + + ++ + T LVSV++ + +G + PI ++ + +GA DA Q
Sbjct: 176 VQLTPEEEFDFGHYQSLVNEKTKLVSVVHVSNTLGCINPIKDIVGLAHQYGAKVLVDACQ 235
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALY--LRPGVELMPLLGGGGQ--ENGLRS 233
A LPLDVQ L D L S HK+ G G LY L + P LGGG + L +
Sbjct: 236 SAPHLPLDVQDLDCDWLVASGHKMCAPTGIGFLYGKLNLMRSMPPFLGGGEMIADVFLEN 295
Query: 234 GTQA---------TPAIA---GFGVAAE-LAAQELETETGRLILLRDRLFAILADVPGLI 280
T A TPAIA G A + L ++ L LF L +P +
Sbjct: 296 STYADLPHKFEAGTPAIAEAIALGAAVDYLTNIGMDKIHAYEAELTTYLFEKLMQIPQVK 355
Query: 281 PTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
G K P L + ++ L L+ AGI I AG C
Sbjct: 356 IYGPK----PPKERAALASFTSGEVHPHDLSTMLDQAGIAIRAGHHC 398
>Cha6605_2881 cysteine desulfurase family protein, VC1184 subfamily
Length = 415
Score = 58.2 bits (139), Expect = 4e-10, Method: Compositional matrix adjust.
Identities = 48/200 (24%), Positives = 78/200 (39%), Gaps = 1/200 (0%)
Query: 4 YLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPES 63
+ D T IA + L N R ++ R A L+
Sbjct: 27 FFDGPGGTQVPRNTIAAMSDYLIYSNANAHGAFITSERTDKLINETRSAAADLLGCAANE 86
Query: 64 IIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN-H 122
I+F + T + R +I + ++H A P + LE G I L +
Sbjct: 87 IVFGANMTTLAFAFSRAIGRELQPGDEIITTKLDHYANISPWQALEEKGVVIRSLDIRVE 146
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
++ + L+ L T LV++ Y + +GTV +A + K+ GAL DAV
Sbjct: 147 DCTLDLDRLQELLTDRTKLVAIGYASNAVGTVNDVAAVTKLAHQVGALVFVDAVHYVPHG 206
Query: 183 PLDVQTLPVDLLSLSSHKLY 202
+DVQ L D L+ S++K +
Sbjct: 207 AIDVQELDCDFLACSAYKFF 226
>Cha6605_3714 selenocysteine lyase
Length = 385
Score = 44.7 bits (104), Expect = 8e-06, Method: Compositional matrix adjust.
Identities = 44/176 (25%), Positives = 76/176 (43%), Gaps = 15/176 (8%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEH-SAISEPAKML 108
R ++A + ++I T T N+A+ G+ + +++S EH I+ ++
Sbjct: 62 RQEIAAELGVTADTIALTENVTAGCNIALWGLN--WQAGDRILLSDCEHPGVIASINEVC 119
Query: 109 ENWGWEITRLGVNHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKIT 164
+G + + Q +N D + A L T LV + + G V P+AE+ K
Sbjct: 120 RRFGVAVDLFPL--QATLNTGDPVATIVAHLTPQTRLVVLSHVLWNTGQVVPLAEMSKAI 177
Query: 165 KTHGALFH-----TDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRP 214
+ + DA Q G LPL + L +D + + HK + G G G LY+ P
Sbjct: 178 RQYPDAEQPIRILVDAAQSVGLLPLQLGELGIDFYAFTGHKWWCGPEGVGGLYIHP 233
>Cha6605_5618 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 380
Score = 38.5 bits (88), Expect = 8e-04, Method: Compositional matrix adjust.
Identities = 23/80 (28%), Positives = 41/80 (51%), Gaps = 4/80 (5%)
Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+L+AA+ H ++ +++ ++ G QPI +G++ + + L D V G +P+ +
Sbjct: 137 EELRAAMAEHRPKILGLVHAETSTGCRQPIDGVGELCREYDCLLLLDTVTSLGGVPIHLD 196
Query: 188 TLPVDLLSLSSHKLYGGLGA 207
VDL S K GLG
Sbjct: 197 KWGVDLAYSCSQK---GLGC 213
>Cha6605_4341 selenocysteine lyase
Length = 385
Score = 35.0 bits (79), Expect = 0.010, Method: Compositional matrix adjust.
Identities = 32/119 (26%), Positives = 55/119 (46%), Gaps = 9/119 (7%)
Query: 112 GWEITRLGVNHQGRVNPEDLKAALRHNTVLVS---VIYGQSEIGTVQPIAELGKITKTHG 168
G+EI + + + ++ D+ + +V+ V Y S ++PI E G
Sbjct: 124 GFEIIEIKASERMQIETSDVMDRIDDRVAVVALSLVSYINSACLDIKPIIEAAH---AAG 180
Query: 169 ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRPGV--ELMPLLGG 224
A+ DA Q G LP+DV +L D++ +K L GG+G Y+ + +L P+ G
Sbjct: 181 AIVILDAYQAVGVLPIDVISLGADVVLGGMNKWLCGGIGLAFAYINSSLSEQLDPVYPG 239
>Cha6605_4439 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 377
Score = 29.3 bits (64), Expect = 0.67, Method: Compositional matrix adjust.
Identities = 20/81 (24%), Positives = 38/81 (46%), Gaps = 4/81 (4%)
Query: 126 VNPEDLKAALRHNTVL----VSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
++P ++AAL + V V + ++ G + + + K K HGAL DAV G
Sbjct: 110 IDPTTVQAALDADPDKAIKGVIVTHSETSTGVINDLETISKHVKAHGALIIVDAVTSLGA 169
Query: 182 LPLDVQTLPVDLLSLSSHKLY 202
+ + + +D++ S K +
Sbjct: 170 VNVPIDEWGLDVVCSGSQKGF 190
Database: GCA_000317145.1P
Posted date: Aug 27, 2016 11:14 PM
Number of letters in database: 1,793,467
Number of sequences in database: 5945
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5945
Number of Hits to DB: 1,366,532
Number of extensions: 58680
Number of successful extensions: 194
Number of sequences better than 1.0: 9
Number of HSP's gapped: 187
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,793,467
Length adjustment: 87
Effective length of query: 295
Effective length of database: 1,276,252
Effective search space: 376494340
Effective search space used: 376494340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)