BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000317125.1P
5752 sequences; 1,839,647 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Chro_3394 Cysteine desulfurase 605 0.0
Chro_0154 Cysteine desulfurase 286 2e-93
Chro_4587 cysteine desulfurase NifS 281 5e-91
Chro_2003 cysteine desulfurase 84 2e-18
Chro_5578 aminotransferase class V 58 4e-10
Chro_4470 L-2,4-diaminobutyrate decarboxylase 43 3e-05
>Chro_3394 Cysteine desulfurase
Length = 391
Score = 605 bits (1559), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 292/382 (76%), Positives = 340/382 (89%), Gaps = 1/382 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTR EAI+ +Q +LTQQWGNPSSLHEWG RAA +LE ARMQVA L+NA
Sbjct: 1 MQIYLDYSATTPTRLEAISTMQAVLTQQWGNPSSLHEWGGRAATVLEQARMQVASLVNAR 60
Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
ESIIFTSGGTEADNLAIMGVA+ Y++PQH+IISS+EHSAI+EPA++LE WGW++TRL
Sbjct: 61 NAESIIFTSGGTEADNLAIMGVAQRYSSPQHLIISSIEHSAIAEPARLLERWGWQVTRLP 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ G VNP+ L+AAL+ NTVLVS++YGQSE+GT+QPI LGKIT++HGALFHTDAVQVA
Sbjct: 121 VDGNGCVNPDRLQAALQPNTVLVSIVYGQSEVGTLQPIETLGKITRSHGALFHTDAVQVA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP+D+Q LPVDLLSLSSHK+YG GAGALY+RPGVE++PLLGGGGQE+ LRSGTQA P
Sbjct: 181 GRLPIDLQELPVDLLSLSSHKIYGPQGAGALYIRPGVEIVPLLGGGGQESKLRSGTQAVP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
IAGFG+AAELAAQE+ TET RL+ LRDRLFA LADVP LIPTGD+ RLPHH SFY++
Sbjct: 241 IIAGFGIAAELAAQEMPTETSRLVSLRDRLFAQLADVPQLIPTGDRQSRLPHHASFYIKG 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADGEK+SGKT+VRQ+NLAGIGI AG+AC+SGKLSPSPILLAMGY + AAL GIRLTLG++
Sbjct: 301 ADGEKVSGKTIVRQMNLAGIGIGAGSACHSGKLSPSPILLAMGYDKAAALSGIRLTLGRE 360
Query: 360 TTAADIDWTGIVVKQILQRLIP 381
TTAADIDWT V+KQIL RLIP
Sbjct: 361 TTAADIDWTATVLKQILDRLIP 382
>Chro_0154 Cysteine desulfurase
Length = 392
Score = 286 bits (733), Expect = 2e-93, Method: Compositional matrix adjust.
Identities = 160/370 (43%), Positives = 228/370 (61%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
+YLD +TTP + + + +GNPSS H +G A + AR +A I A P
Sbjct: 5 IYLDCHSTTPLDERVLQAMLPYFREHFGNPSSNSHAYGWEAEAAVRQAREILAEAIAATP 64
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y +H+I + EH+A+ +P++ L + G+E+T L V
Sbjct: 65 EEIVFTSGATEANNLAIKGVAEAYFQKGRHIITLATEHNAVIDPSEYLRSLGFEVTFLPV 124
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ E L+ A R +T+L+SV+ +EIG +QP+AE+GK+ + LFHTDA Q G
Sbjct: 125 QIDGLIDLEKLEQAFRPDTILISVMAANNEIGVLQPLAEIGKMCRDRQVLFHTDAAQAIG 184
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ + +DL+SL++HK+YG G GALY+R P V+L P L GGGQE G+RSGT
Sbjct: 185 KIPLDVQAMQIDLMSLTAHKVYGPKGIGALYVRRRNPRVQLAPQLHGGGQERGMRSGTLY 244
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A +A E ETE R + LRDRL+ L+ + G+ G RLP +L+ +
Sbjct: 245 TPQIVGFGQAVAIALAERETEAKRQMQLRDRLWQKLSQLDGVYLNGHPSQRLPGNLNISV 304
Query: 298 EAADGEKISGKTLVRQLNLAG-IGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L + +S+GAAC+S K +PS +LLA+G S++ A IR +
Sbjct: 305 EGVDGTAL-------HLGLQPVVAVSSGAACSSTKTAPSHVLLALGRSEQLAYASIRFGI 357
Query: 357 GKQTTAADID 366
G+ T +ID
Sbjct: 358 GRFNTIEEID 367
>Chro_4587 cysteine desulfurase NifS
Length = 407
Score = 281 bits (718), Expect = 5e-91, Method: Compositional matrix adjust.
Identities = 156/384 (40%), Positives = 224/384 (58%), Gaps = 12/384 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE + + L++ +GNPSS+H +G + +++ AR QVA LI A
Sbjct: 5 IYLDNNATTRVDPEVVEAMLPYLSEYYGNPSSMHSFGGQVGKVVKQARQQVAALIGADDS 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
IIFTS GTE DN AI + +H++ + VEH A+ K LE G+ +T L VN
Sbjct: 65 EIIFTSCGTEGDNAAIRAALQAQPDKRHIVTTQVEHPAVLNVCKQLETQGYTVTYLSVNR 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ ++L A++ NT LV+++Y +E G V PI ++G K +GA+FH DAVQ G++
Sbjct: 125 QGQIDLDELAASMTGNTALVTIMYANNETGVVFPIEQIGLRVKEYGAIFHVDAVQAVGKI 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T VDLL+LS HKL+ G GALY+R GV PLL GG QE G R+GT+ P I
Sbjct: 185 PLNMKTSTVDLLTLSGHKLHAPKGIGALYVRRGVRFRPLLVGGHQERGRRAGTENVPGIV 244
Query: 243 GFGVAAELAAQELETETGRLILLRDRL-FAILADVPGLIPTGD------KIHRLPHHLSF 295
G AAE+ L T + LRDRL +L +P GD + +RLP+ +
Sbjct: 245 ALGKAAEIELAHLAATTKKQKQLRDRLEKTLLETIPDCEVNGDVFDGKLRKNRLPNTTNI 304
Query: 296 YLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLT 355
+ +GE I + LN GI S+G+AC SG L PS +L +MG G IR +
Sbjct: 305 GFKYIEGEAI-----LLSLNQFGICASSGSACTSGSLEPSHVLRSMGLPYTTLHGSIRFS 359
Query: 356 LGKQTTAADIDWTGIVVKQILQRL 379
L + TT A++D V+ I++RL
Sbjct: 360 LSRYTTDAEVDAVLAVMPSIVERL 383
>Chro_2003 cysteine desulfurase
Length = 420
Score = 83.6 bits (205), Expect = 2e-18, Method: Compositional matrix adjust.
Identities = 67/229 (29%), Positives = 106/229 (46%), Gaps = 6/229 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ + ++ Q N +H +A E AR +VA +NA
Sbjct: 30 VYLDNAATSQKPLLVLNTLRNYYEQYNSNVHRGVHTLAAKATEAYEGARDKVAAFVNAAQ 89
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITRL 118
E I+FT TEA NL +I+S +EH + P ++L + G + +
Sbjct: 90 REEIVFTRNATEAINLVAYSWGNSNIQRGDEIILSVMEHHSNLVPWQLLAQRTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ + + E K+ + T LV+V++ + +G + PI E+ I HGA DA Q
Sbjct: 150 ELTPEEEFDLEQYKSLISDKTKLVAVVHVSNTLGCINPIQEIIAIAHQHGAKVLIDACQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
+P++VQ + D L S HK+ G G LY + + + P LGGG
Sbjct: 210 VPHMPVNVQQMDCDWLVASGHKMCAPTGIGFLYGKLALLRSMPPFLGGG 258
>Chro_5578 aminotransferase class V
Length = 415
Score = 58.2 bits (139), Expect = 4e-10, Method: Compositional matrix adjust.
Identities = 48/178 (26%), Positives = 82/178 (46%), Gaps = 14/178 (7%)
Query: 47 ETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHS-AISEPA 105
E R +A +NA PE+I T T N+A+ G+ + H+++S EH I+
Sbjct: 78 EKTRQAIATELNAPPETITLTEDVTVGCNIALWGID--WQAKDHILLSDCEHPGVIATTQ 135
Query: 106 KMLENWGWEITRLGVNHQGRVNPEDLKAALRHN----TVLVSVIYGQSEIGTVQPIAELG 161
++ +G E++ + +N D A + + T LV + + G V P+ ++
Sbjct: 136 EITRRFGVEVSTCPL--MATLNQGDPAAVIAQHLTPRTRLVVISHILWNTGQVLPVDKIV 193
Query: 162 KITKTHG----ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
++ + H DA Q G LPL++ L D + + HK + G G G LY+RP
Sbjct: 194 EVCRQHQGDRPVQILVDAAQSVGLLPLNLTQLGADFYAFTGHKWMCGPAGVGGLYVRP 251
>Chro_4470 L-2,4-diaminobutyrate decarboxylase
Length = 502
Score = 43.1 bits (100), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 44/187 (23%), Positives = 80/187 (42%), Gaps = 33/187 (17%)
Query: 67 TSGGTEADNLAIMGVARCYTTPQH-----------------MIISSVEHSAISEPAKMLE 109
TSGGT+++ +A+M +AR Y Q ++ S V H ++ + A +L
Sbjct: 147 TSGGTQSNFMALM-LARDYALQQRGFTSQLHGLPNLAGRFRILCSEVAHFSVQQSAAILG 205
Query: 110 NWGWEITRLGVNHQGRVNPEDLKAAL----RHNTVLVSVIY--GQSEIGTVQPIAELGKI 163
+ R+ V+ R+ + L L R + + + ++ G ++ G++ PIAE+ I
Sbjct: 206 LGMNAVVRVKVDKNYRLCSQHLVQCLEDIDRQDLIPICIVATAGTTDFGSIDPIAEMTAI 265
Query: 164 TKTHGALFHTDAVQVAGRLPLDVQTLPV------DLLSLSSHKLYGGLGAGALYL---RP 214
H H DA + D + D +++ HKL+ +L+L +
Sbjct: 266 AHEHNTWLHVDAAYGGALMLSDRHRQKLAGIHQADSITIDFHKLFYQPIPCSLFLLKDKS 325
Query: 215 GVELMPL 221
ELM L
Sbjct: 326 RFELMRL 332
Database: GCA_000317125.1P
Posted date: Aug 27, 2016 10:57 PM
Number of letters in database: 1,839,647
Number of sequences in database: 5752
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5752
Number of Hits to DB: 1,409,538
Number of extensions: 60930
Number of successful extensions: 198
Number of sequences better than 1.0: 9
Number of HSP's gapped: 190
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,839,647
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,333,471
Effective search space: 392040474
Effective search space used: 392040474
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)