BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000316685.1P
3545 sequences; 1,048,297 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Syn6312_1067 cysteine desulfurase family protein 503 e-178
Syn6312_2950 cysteine desulfurase family protein 269 4e-87
Syn6312_3385 cysteine desulfurase-like protein, SufS subfamily 87 6e-20
Syn6312_2638 cysteine desulfurase-like protein, SufS subfamily 75 2e-15
Syn6312_0107 selenocysteine lyase 69 7e-14
Syn6312_3153 serine-pyruvate aminotransferase/archaeal aspartate... 40 1e-04
>Syn6312_1067 cysteine desulfurase family protein
Length = 387
Score = 503 bits (1295), Expect = e-178, Method: Compositional matrix adjust.
Identities = 253/379 (66%), Positives = 302/379 (79%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTP RPEA+A + ++ Q WGNPSSLHEWG RAA +LE ARMQVA LIN+
Sbjct: 1 MQIYLDYSATTPCRPEAMASMNRVMAQAWGNPSSLHEWGQRAATVLEQARMQVASLINSS 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P I+FTSGGTE++NLAI GVAR +++PQH+IISSVEH+A+S+P LE+ GW++TRL V
Sbjct: 61 PLGIVFTSGGTESNNLAIYGVARQFSSPQHLIISSVEHAAVSQPMAFLESQGWQVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ GRVNP DL A++ NTVLVSVIYGQSE+GT+QPI+EL KI + LFHTDAVQVAG
Sbjct: 121 DRWGRVNPRDLDQAIQANTVLVSVIYGQSEVGTLQPISELAKICRNRDVLFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLP+DVQ L VDLLSLSSHKLYG G GALY+RPGVEL P L GG QE+GLR+GTQA P
Sbjct: 181 RLPVDVQALGVDLLSLSSHKLYGPQGVGALYIRPGVELHPFLAGGMQESGLRAGTQALPN 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFG+AAELA EL E RL LR+RLFA L P L PTG +++RLPHHLSF + A
Sbjct: 241 IAGFGIAAELAEVELLEEIARLQTLRERLFAQLYACPQLQPTGHRLYRLPHHLSFTVIDA 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
G +SGKTLVRQLNLAGIG+S+G+ACNSGK PSP+L AMGY A +RLTLGK T
Sbjct: 301 YGTPLSGKTLVRQLNLAGIGVSSGSACNSGKSIPSPVLRAMGYDDGLAKSSLRLTLGKHT 360
Query: 361 TAADIDWTGIVVKQILQRL 379
TAADIDW G+V+ Q+++R+
Sbjct: 361 TAADIDWVGLVLPQVIERV 379
>Syn6312_2950 cysteine desulfurase family protein
Length = 384
Score = 269 bits (688), Expect = 4e-87, Method: Compositional matrix adjust.
Identities = 162/381 (42%), Positives = 226/381 (59%), Gaps = 13/381 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD+ ATTP PE +A + ++ GNPSS H +G AA ++ AR +A INA
Sbjct: 7 IYLDHQATTPLHPEVLAAMLPYFIEKPGNPSSNGHLYGWEAAAAVKKARATIAKAINAPL 66
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
ES+IFTSG TEA+NL I G+A Y +H++ EH+A+ P + LE G+ +T L V
Sbjct: 67 ESLIFTSGATEANNLVIKGIAEAYFAQGRHIVTVQTEHAAVLAPCRYLEQHGFSVTYLPV 126
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G V+ L LR +T+LVSV+ +EIG +QPI ++ + ++ G L HTDA Q G
Sbjct: 127 QADGFVDLNQLTEVLREDTLLVSVMAANNEIGVLQPIEKISALCRSRGILCHTDAAQALG 186
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG---VELMPLLGGGGQENGLRSGTQA 237
++PLDVQ VD +SL++HKLYG G G LY++P + L P L GGGQE LRSGT
Sbjct: 187 KIPLDVQAWGVDFMSLTAHKLYGPKGIGGLYIKPQHPPLNLTPQLHGGGQEENLRSGTLP 246
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A LA Q LETE+ RL LR++L+ L + +IP G LP LS
Sbjct: 247 TPQIVGFAQAVTLAHQSLETESLRLTALREKLWQGLEQLGEVIPNGHPTQSLPGCLSVSF 306
Query: 298 EAADGEKISGKTLVRQLNLAG-IGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
+ DG K+ Q NL G + +SAG+AC+S K PS +L+A+G + +R L
Sbjct: 307 QGLDGAKL-------QSNLQGKLALSAGSACSSAKPEPSHVLIALGRTVAECRATLRFGL 359
Query: 357 GKQTTAADIDWTGIVVKQILQ 377
G+ TT+A+I+ T ++ +Q
Sbjct: 360 GRSTTSAEIEQTLEILTDTIQ 380
>Syn6312_3385 cysteine desulfurase-like protein, SufS subfamily
Length = 419
Score = 87.4 bits (215), Expect = 6e-20, Method: Compositional matrix adjust.
Identities = 69/232 (29%), Positives = 117/232 (50%), Gaps = 12/232 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHE----WGNRAALILETARMQVAGLIN 58
VYLD +AT+ +P+A+ IQT++ G +++H ++A E AR +VA IN
Sbjct: 30 VYLDNAATS-QKPQAV--IQTLVDYYQGYNANVHRGVHTLSDKATTAYEGARDKVAKFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T +EA NL + P +I++ EH + P +++ + G +
Sbjct: 87 ARSRQEIVYTRNASEAINLVAYSWGLSHLGPGDEIILTVFEHHSNLIPWQLVAQKTGAIL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ ++ Q N ++ L T LV+V++ + +G + P+A++ ++ GA DA
Sbjct: 147 KFVELDAQQSFNFPQFQSLLSDKTKLVAVVHVSNTLGCITPVAQIAELAHAVGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
Q A + LDVQ L D L S HK+ G G LY + + + P LGGG
Sbjct: 207 CQSAPHMKLDVQALDCDWLVASGHKMCAPTGIGFLYGKLDLLRSMPPFLGGG 258
>Syn6312_2638 cysteine desulfurase-like protein, SufS subfamily
Length = 759
Score = 74.7 bits (182), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 94/354 (26%), Positives = 149/354 (42%), Gaps = 43/354 (12%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNP--SSLHEWGNRAALILETARMQVAGLINAV 60
++ D +ATT +P+A+ T + + + HE R+ E +R QV INA
Sbjct: 376 IWFDNAATT-HKPKAVIDRLTYYYEHENSNVHRAAHELAARSTDAYEKSREQVRRFINAS 434
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEP-AKMLENWGW 113
I+F G TEA NL VA+ + + +I++ +EH A P ++ G
Sbjct: 435 STNEIVFVRGTTEAINL----VAKSWGSQNVHEGDEIILTHLEHHANIVPWQQLCAEKGA 490
Query: 114 EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173
+ + V+ G+V ++ + L T +V+ + + +GTV P AE+ + +GA
Sbjct: 491 RLRIVPVDDHGQVRLDEYQKLLSDRTKIVAFTHVSNALGTVTPAAEMIALAHRYGAKVLL 550
Query: 174 DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG----- 226
D Q + +DVQ L D S HK++G G G +Y + + +P GG
Sbjct: 551 DGAQSVSHMAIDVQALDCDWFVFSGHKVFGPTGIGVVYGKEDLLNATLPWQTGGNMIVDV 610
Query: 227 --------QENG-LRSGTQATPAIAGFGVAAELAAQELETETGRLILLRDRLF----AIL 273
Q G +GT G G A E Q++ E+ + L A+L
Sbjct: 611 TFEKTVYHQAPGRFEAGTGNIADAVGLGAALEY-VQKIGLES--ICRYEHELLVYGTALL 667
Query: 274 ADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
++PGL G + LSF LE E + LN GI + AG C
Sbjct: 668 QEIPGLRLIGTAPEKA-AVLSFVLEGHSPEAVGSA-----LNQEGIAVRAGHHC 715
>Syn6312_0107 selenocysteine lyase
Length = 392
Score = 69.3 bits (168), Expect = 7e-14, Method: Compositional matrix adjust.
Identities = 65/255 (25%), Positives = 109/255 (42%), Gaps = 27/255 (10%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE 109
R ++ +N P+++ T T N+ + G+ + H++++ EH + + L+
Sbjct: 69 RQALSQELNITPDTLALTDSVTTGCNIVLWGLD--WRAGDHLLLTDCEHPGVLAIGQQLQ 126
Query: 110 -NWGWEITRLGVNHQGRVNPEDLKAALRH----NTVLVSVIYGQSEIGTVQPIAELGKIT 164
+G ++T L + Q + +PE L R T LV++ + GT P+ E+
Sbjct: 127 ARFGIDLTVLPLLQQAQADPEHLVTCFRQALTPRTRLVALSHLLWNTGTTLPLKEIVATC 186
Query: 165 KTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGL-GAGALYLRPGV--ELMP- 220
G L DA Q G +PLD+ L VD + + HK + G G G LY+ P L P
Sbjct: 187 HKQGVLVLADAAQSVGMMPLDLPGLGVDFYAFTGHKWFCGPDGLGGLYVHPEQLGHLQPT 246
Query: 221 LLGGGGQENG--------------LRSGTQATPAIAGFGVAAELAAQELETET--GRLIL 264
+G +NG T A P + G A + Q T T ++
Sbjct: 247 YVGWRSIKNGPQGFPESFQATAAKFEIATTAFPLVPGLTAALDFHRQAGTTPTRYEQICE 306
Query: 265 LRDRLFAILADVPGL 279
L L+ LA++PG+
Sbjct: 307 LSTYLWQGLANIPGV 321
>Syn6312_3153 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 382
Score = 40.0 bits (92), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 42/155 (27%), Positives = 68/155 (43%), Gaps = 15/155 (9%)
Query: 126 VNPEDLKAALRHNTV----LVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
+NP+D KAAL +T V + + ++ G + + + K HGAL DAV G
Sbjct: 115 LNPDDFKAALEADTAKTIKAVVITHSETSTGVLNDLEAINHHVKAHGALIIVDAVTSLGA 174
Query: 182 LPLDVQTLPVDLLSLSSHKLY---GGLG-------AGALYLRPGVELMPLLGGGGQENGL 231
+ + V +D++ S K Y GLG A Y + L G +++
Sbjct: 175 VSVPVDAWGLDVVGSGSQKGYMMPPGLGFVSVSAKAWEAYTTAKLPKFYLDLGKYRKDAA 234
Query: 232 RSGTQATPAIAGFGVAAELAAQELETETGRLILLR 266
++ T TPAI F A ++A + ++ E I R
Sbjct: 235 KNTTPYTPAINLF-FALDVALEIMKAEGLENIFAR 268
Database: GCA_000316685.1P
Posted date: Aug 27, 2016 10:28 PM
Number of letters in database: 1,048,297
Number of sequences in database: 3545
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3545
Number of Hits to DB: 840,163
Number of extensions: 37483
Number of successful extensions: 143
Number of sequences better than 1.0: 8
Number of HSP's gapped: 138
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 1,048,297
Length adjustment: 84
Effective length of query: 298
Effective length of database: 750,517
Effective search space: 223654066
Effective search space used: 223654066
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)