BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000316645.1P
5449 sequences; 1,828,442 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Nos7524_0612 cysteine desulfurase family protein 627 0.0
Nos7524_1240 cysteine desulfurase NifS 294 3e-96
Nos7524_1329 cysteine desulfurase family protein 289 2e-94
Nos7524_2580 cysteine desulfurase family protein 234 2e-73
Nos7524_4037 cysteine desulfurase-like protein, SufS subfamily 84 1e-18
Nos7524_1464 selenocysteine lyase 68 3e-13
Nos7524_1152 cysteine desulfurase family protein, VC1184 subfamily 67 1e-12
Nos7524_0255 serine-pyruvate aminotransferase/archaeal aspartate... 38 0.001
Nos7524_1419 selenocysteine lyase 37 0.002
>Nos7524_0612 cysteine desulfurase family protein
Length = 389
Score = 627 bits (1618), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 306/383 (79%), Positives = 340/383 (88%), Gaps = 1/383 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA- 59
MQ+YLDYSATTPTRPEAIA +Q LTQQWGNPSSLHEWG RAA I+E AR+QVAGLINA
Sbjct: 1 MQIYLDYSATTPTRPEAIAAMQAALTQQWGNPSSLHEWGQRAATIVEQARLQVAGLINAA 60
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
PESIIFTSGGTE++NL +MG+AR Y PQH+IIS+VEHSAI+EPAK+LE WGWE+TRL
Sbjct: 61 TPESIIFTSGGTESNNLVMMGIARLYAVPQHIIISTVEHSAIAEPAKLLEKWGWEVTRLA 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ +GRVNP+ LKAALRHNTVLVS+IYGQSEIGTVQPI ELGKI + HGALFHTDAVQ
Sbjct: 121 VDSKGRVNPQTLKAALRHNTVLVSIIYGQSEIGTVQPITELGKIAQQHGALFHTDAVQAV 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP+DVQ LPVDLLSLSSHKLYG GAGALY+RP VEL+PLLGGGGQE GLRSGTQA P
Sbjct: 181 GRLPIDVQQLPVDLLSLSSHKLYGPQGAGALYIRPDVELIPLLGGGGQERGLRSGTQALP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
AIAGFGVAAELAAQEL ET RLI LRDR+F++LADVPGLIPTGD HRLPHH+SF LE
Sbjct: 241 AIAGFGVAAELAAQELPQETPRLIKLRDRIFSLLADVPGLIPTGDLEHRLPHHVSFSLEY 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADG+K+SGKTLVRQLNLAGIGISAG+AC+SGKLSPSPILLAMGY + AA GGIRLTLG+
Sbjct: 301 ADGKKLSGKTLVRQLNLAGIGISAGSACHSGKLSPSPILLAMGYPETAAFGGIRLTLGRD 360
Query: 360 TTAADIDWTGIVVKQILQRLIPD 382
TT ADIDWT +V+KQ+LQRL D
Sbjct: 361 TTQADIDWTAMVLKQVLQRLTSD 383
>Nos7524_1240 cysteine desulfurase NifS
Length = 400
Score = 294 bits (752), Expect = 3e-96, Method: Compositional matrix adjust.
Identities = 163/378 (43%), Positives = 225/378 (59%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE + I LT+ +GNPSS+H +G + A + TAR QVA L+ A
Sbjct: 4 IYLDNNATTKVDPEVVEAIMPYLTEYYGNPSSMHTFGGQLAKAVRTAREQVAALLGADES 63
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 64 EIVFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYSVTYLSVNR 123
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ ++L+A+L NT LV+++Y +E GTV PI ++G K GA+FH DAVQ G++
Sbjct: 124 QGQLDLDELEASLTGNTALVTIMYANNETGTVFPIEQIGMRVKEKGAIFHVDAVQAVGKI 183
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T VD+L++S HK++ G G LY+R GV PL+ GG QE G R+GT+ P I
Sbjct: 184 PLNMKTSTVDMLTMSGHKIHAPKGIGVLYVRRGVRFRPLMIGGHQERGRRAGTENVPGIV 243
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G G AAEL LE T R LRDRL +LA +P GD RLP+ + + +
Sbjct: 244 GLGKAAELEMLHLEEATTREKRLRDRLEQTLLAKIPECEVNGDITQRLPNTTNIGFKYIE 303
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 304 GEAI-----LLLLNKYGICASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLCRYTT 358
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ +I++RL
Sbjct: 359 EAEIDKVIEVMPEIVERL 376
>Nos7524_1329 cysteine desulfurase family protein
Length = 388
Score = 289 bits (739), Expect = 2e-94, Method: Compositional matrix adjust.
Identities = 161/370 (43%), Positives = 230/370 (62%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP + + T+++GNPSS+ H +G ++ +R +A INA P
Sbjct: 6 IYLDCHATTPVDERVLTTMLPYFTEKFGNPSSISHVYGWETEAAVKQSREILAAAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLAI GVA Y QH++ + EH+A+ +P + L+N G++IT L V
Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFQKGQHIVTVATEHNAVLDPCEYLKNLGFDITILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ ++L+ ALR T+LVSV+ +EIG +QP+AE+G I +FHTDA Q G
Sbjct: 126 KPDGLIDLKELETALRPETILVSVMAANNEIGVLQPLAEIGAICHDRQIIFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDVQ + +DL+S+++HK+YG G GALY+R P V+L P GGG E G+RSGT
Sbjct: 186 KIPLDVQAMHIDLMSITAHKVYGPKGIGALYVRRRNPKVQLAPQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A E ETET RL LR L+ L+ + G+ G RL +L+ +
Sbjct: 246 TPQIVGFGKAVEIALAEQETETQRLTQLRQSLWDQLSHLEGIYLNGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG +S L L + +S+G+AC+S K +PS +L A+G+S+K A IR +
Sbjct: 306 EGVDGAALS-------LGLQPVMAVSSGSACSSTKTAPSHVLTALGHSEKLAYASIRFGI 358
Query: 357 GKQTTAADID 366
G+ T A+ID
Sbjct: 359 GRFNTQAEID 368
>Nos7524_2580 cysteine desulfurase family protein
Length = 382
Score = 234 bits (597), Expect = 2e-73, Method: Compositional matrix adjust.
Identities = 139/366 (37%), Positives = 202/366 (55%), Gaps = 9/366 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY +TTP P + +T +GN +S+ H++GN AA ++ AR Q+A LINA P
Sbjct: 5 IYLDYHSTTPVDPRVAKKVMYYMTTAFGNANSVDHDYGNIAAKAVKQARQQIAELINASP 64
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW-EITRLGV 120
+ IIFTSG TE+ NL I G TP +I+S VEH A+ + K L G EI L V
Sbjct: 65 KEIIFTSGATESINLVIQGQIAQQNTPAKIIVSPVEHKAVIDTCKALVKKGLVEIIWLKV 124
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N Q +++ E L+ L+ ++ +E+GT+ PI ++G I +H F DA Q G
Sbjct: 125 NQQAQIDLEHLEKVCADGAALLCMMAANNEVGTIYPIKKIGAIASSHNIPFLCDASQAVG 184
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
++PL+ Q + L++S+HKLY G G L +R + P++ GGG + G+RSGT P
Sbjct: 185 KIPLNFQDWGITYLAISAHKLYAPKGVGVLVVRKNHPVPPMIYGGGHQQGMRSGTLNVPG 244
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRLPHHLSFYLEA 299
I G G A L E+E + + +LRD+L L +P L GD HRL +L +
Sbjct: 245 IVGLGEACRLRRLEMEQDENAIAILRDQLQNQLQTAIPDLAVNGDLNHRLSGNLHISIPD 304
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
I + VRQ + IS GAAC+SG ++PS +L AM S+ G +R+ +GK
Sbjct: 305 ISNTAIIAR--VRQ----QLAISTGAACSSGAIAPSHVLQAMNLSENIIEGALRIGIGKF 358
Query: 360 TTAADI 365
TT +I
Sbjct: 359 TTQQEI 364
>Nos7524_4037 cysteine desulfurase-like protein, SufS subfamily
Length = 420
Score = 84.3 bits (207), Expect = 1e-18, Method: Compositional matrix adjust.
Identities = 66/230 (28%), Positives = 109/230 (47%), Gaps = 5/230 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ + ++ + N H +A E AR ++A INA
Sbjct: 30 VYLDNAATSQKPFFVLNALRDYYEEYNANVHRGAHTLSAKATDAYEGARDKIAKFINAAS 89
Query: 62 -ESIIFTSGGTEADNL-AIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRL 118
+ I++T +EA NL A +I+S +EH + P +++ + G + +
Sbjct: 90 RQEIVYTRNASEAINLVAYSWGMNNLKAGDEIILSVMEHHSNIVPWQLVAQKTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ + + E K + T LVS+++ + +G + P+AE+ KI +GA F DA Q
Sbjct: 150 ELTPEETFDLEQFKQLISDKTKLVSIVHVSNTLGCINPVAEIAKIAHRYGAKFLLDACQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR-PGVELMPLLGGGGQ 227
+P+DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 210 VPHMPVDVQQIDCDWLVASGHKMCATTGIGFLYGKLELLEAMPPFFGGGE 259
>Nos7524_1464 selenocysteine lyase
Length = 392
Score = 67.8 bits (164), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 51/177 (28%), Positives = 91/177 (51%), Gaps = 10/177 (5%)
Query: 46 LETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHS-AISEP 104
++T R +A +N ++I FT T N+A+ G++ + H+++S EH I+
Sbjct: 68 IQTVRKAIASELNVTSDTIAFTDNVTVGCNIALWGIS--WQAGDHLLLSDCEHPGVIAAT 125
Query: 105 AKMLENWGWEITRLGVN---HQGRVNPEDLKAA-LRHNTVLVSVIYGQSEIGTVQPIAEL 160
++ +G E++ + ++G NP + A LR +T LV + + G V P+ ++
Sbjct: 126 QEISRRFGVEVSICPLKATLNEG--NPVAIIAQNLRPHTRLVVLSHVFWNTGQVLPLDKI 183
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRPGV 216
++ + + + DA Q G LPL++ L VD + + HK L G G G LY+RP V
Sbjct: 184 VEVCRNNNSFLLVDAAQSVGALPLNLTELGVDFYAFTGHKWLCGPEGVGGLYVRPEV 240
>Nos7524_1152 cysteine desulfurase family protein, VC1184 subfamily
Length = 454
Score = 66.6 bits (161), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 39/164 (23%), Positives = 77/164 (46%), Gaps = 1/164 (0%)
Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100
R ++ AR+ +A + + ++F + T + +AR +I++ ++H+A
Sbjct: 64 RTDAVITAARVAIADFLGCSSDEVVFGANMTTLTFILSRAIARIIQPGDEIIVTRLDHAA 123
Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159
P LE G I + ++ ++ DL + T LV+V + + +GT+ +A
Sbjct: 124 NVSPWYALEEQGAIIRVVDIHVEDCTLDMSDLAQQINSRTKLVAVSFASNAVGTINDVAT 183
Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
+ ++ GA DAV A P++V L D L+ S++K +G
Sbjct: 184 IVRLAHAVGAWVFVDAVHYAPHAPINVHALDCDFLACSAYKFFG 227
>Nos7524_0255 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 383
Score = 38.1 bits (87), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 23/80 (28%), Positives = 42/80 (52%), Gaps = 4/80 (5%)
Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+L+ AL H+ ++++++ ++ G QP+ +G++ + G L D V G +P+ +
Sbjct: 137 EELRTALEIHHPAILALVHAETSTGARQPLEGVGELCREFGTLLLLDTVTSLGGVPIFLD 196
Query: 188 TLPVDLLSLSSHKLYGGLGA 207
VDL S K GLG
Sbjct: 197 AWGVDLAYSCSQK---GLGC 213
>Nos7524_1419 selenocysteine lyase
Length = 389
Score = 37.4 bits (85), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 48/214 (22%), Positives = 87/214 (40%), Gaps = 28/214 (13%)
Query: 15 PEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEAD 74
P+A+ IQ L Q L +G +L+ AR ++A INA + ++F T
Sbjct: 29 PQAVLAIQQRLRSQLEQ-EPLRFFGREWEPLLDDARSKLAAFINADIQDVVFVPNATTGV 87
Query: 75 NLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRV-------- 126
N + + + ++ ++ E++A + +R G RV
Sbjct: 88 NSVLRSLT--FAPADEILTTNHEYNACRNALDFIA------SRTG----ARVVVAKIPFP 135
Query: 127 --NPEDLKAAL----RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+P+ + AA+ T L + + S+ G + PI EL + + G D G
Sbjct: 136 IESPQQVIAAVLEQVSSQTRLALIDHVTSQTGLIFPIQELVQELQLRGVDTLIDGAHAPG 195
Query: 181 RLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213
+PL+++ + + + HK L GA LY+R
Sbjct: 196 MIPLNLREIGATYYTGNCHKWLCAPKGAAFLYVR 229
Database: GCA_000316645.1P
Posted date: Aug 27, 2016 11:05 PM
Number of letters in database: 1,828,442
Number of sequences in database: 5449
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5449
Number of Hits to DB: 1,406,411
Number of extensions: 60913
Number of successful extensions: 236
Number of sequences better than 1.0: 9
Number of HSP's gapped: 221
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,828,442
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,348,930
Effective search space: 396585420
Effective search space used: 396585420
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)