BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000316625.1P
5237 sequences; 1,703,644 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Nos7107_4482 Cysteine desulfurase 620 0.0
Nos7107_3362 cysteine desulfurase NifS 278 4e-90
Nos7107_2149 Cysteine desulfurase 277 8e-90
Nos7107_4394 Cysteine desulfurase 236 6e-74
Nos7107_1442 cysteine desulfurase 83 3e-18
Nos7107_2635 Cysteine desulfurase 68 3e-13
Nos7107_0366 cysteine desulfurase family protein 60 1e-10
Nos7107_4671 alanine-glyoxylate aminotransferase apoenzyme 38 0.001
Nos7107_3892 Isopenicillin-N epimerase 37 0.002
>Nos7107_4482 Cysteine desulfurase
Length = 389
Score = 620 bits (1599), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 307/383 (80%), Positives = 348/383 (90%), Gaps = 1/383 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTRPEAIA +Q + TQQWGNPSSLHEWG RAA+++E AR+QVA LINA
Sbjct: 1 MQIYLDYSATTPTRPEAIAAMQAVFTQQWGNPSSLHEWGQRAAIVVEQARVQVADLINAT 60
Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
PESI+FT+GGTEADNLAI+GVAR Y PQH+IISSVEHSAISE A+MLE WGWE+T LG
Sbjct: 61 HPESIVFTAGGTEADNLAIIGVARLYPVPQHIIISSVEHSAISETARMLEMWGWEVTTLG 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V++QGR+NP DLKAALRHNTVLVS+IYGQSE+GTVQPI+ELG I ++HGA+FHTDAVQVA
Sbjct: 121 VDNQGRINPLDLKAALRHNTVLVSIIYGQSEVGTVQPISELGNIARSHGAIFHTDAVQVA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP+DVQTLPVDLLS+SSHK+YG GAGALY+RP V++MPLL GGGQE G+RSGTQA P
Sbjct: 181 GRLPIDVQTLPVDLLSISSHKIYGPQGAGALYIRPSVKIMPLLTGGGQEMGMRSGTQAVP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
IAGFGVAAELAA+EL TET RLI LRDRLFA+LAD+PGLIPTGD+IHRLPHH SF LE
Sbjct: 241 MIAGFGVAAELAAKELATETPRLIDLRDRLFALLADIPGLIPTGDRIHRLPHHASFCLEY 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADGEK+SGKTLVRQLNLAGIGISAGAAC+SGKLSPSPILLAMGYSQKAALGGIR+TLG+
Sbjct: 301 ADGEKLSGKTLVRQLNLAGIGISAGAACHSGKLSPSPILLAMGYSQKAALGGIRMTLGRD 360
Query: 360 TTAADIDWTGIVVKQILQRLIPD 382
TT ADIDWT +V++QILQR+ D
Sbjct: 361 TTEADIDWTAMVLQQILQRITAD 383
>Nos7107_3362 cysteine desulfurase NifS
Length = 400
Score = 278 bits (711), Expect = 4e-90, Method: Compositional matrix adjust.
Identities = 157/378 (41%), Positives = 221/378 (58%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P+ + + LT+ + NPSS+H +G + ++ AR Q+A L+ A
Sbjct: 4 IYLDNNATTKVDPQVVEAMMPYLTEYYANPSSMHTFGGQLGKAVKIAREQLAALLGADES 63
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 64 EIVFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYSVTYLSVNG 123
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ ++L+A+L NT LVS++Y +E GT+ PI ++G K GALFH DAVQ G++
Sbjct: 124 QGQLDLDELEASLTGNTALVSIMYANNETGTIFPIEQIGLRVKESGALFHVDAVQAVGKI 183
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T VD+L++S HK++ G GALY+R GV PLL GG QE G R+GT+ P I
Sbjct: 184 PLNMKTSTVDMLTMSGHKIHAPKGIGALYVRRGVRFRPLLLGGHQERGRRAGTENVPGIV 243
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAEL LE R LRDRL +LA +P GD +RLP+ + + +
Sbjct: 244 ALGKAAELELLHLEAARERERQLRDRLEQTLLAKIPDCEVNGDTKNRLPNTTNIGFKYIE 303
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC S L PS +L AMG G IR +L + TT
Sbjct: 304 GEAI-----LLLLNKYGICASSGSACTSDSLEPSHVLRAMGLPYTTLHGSIRFSLCRYTT 358
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ I++RL
Sbjct: 359 DAEIDRVIEVMPDIVERL 376
>Nos7107_2149 Cysteine desulfurase
Length = 388
Score = 277 bits (708), Expect = 8e-90, Method: Compositional matrix adjust.
Identities = 153/370 (41%), Positives = 226/370 (61%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP +A + T+++GNP+S+ H +G A ++ R +A INA P
Sbjct: 6 IYLDCHATTPVDERVLAAMLPYFTEKFGNPASINHVYGWEAEAAVKQTREILAAAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
I+FTSG TEA+NLA+ G+A Y QH+I + EH A+ +P + L++ G+EIT L V
Sbjct: 66 AEIVFTSGATEANNLAVKGIAEAYFQKGQHIITVATEHKAVLDPCEYLKSLGFEITILSV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ +L+ A R T+LVSV+ +EIG +QP+A++GKI + +FHTDA Q G
Sbjct: 126 QKDGLIDLNELEKACRPETILVSVMTANNEIGVLQPLADIGKICRDRNIIFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK+YG G GALY+R P V+L P GGG E G+RSGT
Sbjct: 186 KIPLDVEAMNIDLMSLTAHKVYGPKGIGALYVRRRNPRVQLAPQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A E TET RL LR+ L+ L+ + G+ G RL +L+ +
Sbjct: 246 TPQIVGFGKAVEIALAEQATETARLTQLRESLWLQLSQLAGIHLNGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAG-IGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
DG +S L L I +S+G+AC+S +PS +L A+G S++ A +R +
Sbjct: 306 AGVDGAALS-------LGLQPVIAVSSGSACSSAITAPSHVLTALGNSEQLAYASVRFGI 358
Query: 357 GKQTTAADID 366
G+ T+ +ID
Sbjct: 359 GRFNTSEEID 368
>Nos7107_4394 Cysteine desulfurase
Length = 382
Score = 236 bits (601), Expect = 6e-74, Method: Compositional matrix adjust.
Identities = 143/367 (38%), Positives = 205/367 (55%), Gaps = 9/367 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY +TTP P + +T +GN +S+ H +G+ AA ++ AR Q+A LINA P
Sbjct: 5 IYLDYHSTTPVDPRVAEKVMYYMTTAFGNANSVDHGYGDEAAKAVKQARQQIAELINASP 64
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW-EITRLGV 120
+ IIFTSG TE+ NLAI G TP +IIS VEH A+ + K L G EI L V
Sbjct: 65 KEIIFTSGATESINLAIQGNISQQNTPAKIIISPVEHKAVLDTCKALVKKGLAEIIWLKV 124
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N Q +++ E L+ L+ V+ +E+GT+ PI ++G I ++ F DA Q G
Sbjct: 125 NQQAQIDLEHLEKVCADGAALLCVMAANNEVGTIYPIEKIGAIASSYNIPFLCDASQAVG 184
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
++PL+ Q + L++S HKLY G GAL ++ L P++ GGG E GLRSGT P
Sbjct: 185 KIPLNFQDWGITYLAISGHKLYAPKGVGALVVKRNYHLPPMIYGGGHEQGLRSGTLNVPG 244
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILA-DVPGLIPTGDKIHRLPHHLSFYLEA 299
IAG G A L E+E + + LLRD+L L +P L+ GD +RL +L +
Sbjct: 245 IAGLGEACRLRGLEMERDEKAIALLRDQLQNQLQIAIPDLVVNGDLNNRLSGNLHISITG 304
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
I + VR + IS+GAAC+SG ++PS +L AM S+ G +R+ +GK
Sbjct: 305 IPNSAIIAR--VRH----HLAISSGAACSSGVVAPSHVLQAMNLSENIVEGALRIGIGKF 358
Query: 360 TTAADID 366
TT +I+
Sbjct: 359 TTREEIE 365
>Nos7107_1442 cysteine desulfurase
Length = 420
Score = 83.2 bits (204), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 65/230 (28%), Positives = 106/230 (46%), Gaps = 5/230 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ + ++ Q N H +A E AR ++A INA
Sbjct: 30 VYLDNAATSQKPLLVLNALRNYYEQYNANVHRGAHTLSAKATDAYEAARDKIAQFINAAS 89
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITRL 118
+ I++T +EA NL P +I+S +EH + P + + + G + +
Sbjct: 90 RQEIVYTRNASEAINLVAYSWGMNNLQPGDEIILSVMEHHSNIVPWQFVAQKTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ + + E K + T LVS+++ + +G + P+ E+ IT +GA F DA Q
Sbjct: 150 ELTPEETFDLEQFKQLISEKTKLVSIVHVSNTLGCINPVKEIADITHRYGAKFLVDACQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR-PGVELMPLLGGGGQ 227
+ +DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 210 VPHMKVDVQDIGCDWLVASGHKMCAPTGIGFLYGKLELLEAMPPFFGGGE 259
>Nos7107_2635 Cysteine desulfurase
Length = 395
Score = 67.8 bits (164), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 61/224 (27%), Positives = 105/224 (46%), Gaps = 20/224 (8%)
Query: 2 QVYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSLHEWGNRAALIL----ETARMQVAGL 56
++Y +Y P +A+ AII+T + L +GN + +T + +A
Sbjct: 24 KIYFNYGGQGPLPKKAMDAIIKTE-----NHIQELGPFGNAVGRWIGSYTQTIKQAIASE 78
Query: 57 INAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
+NA PE+I FT T N+ + G+ + H+++S EH + AK + + E+
Sbjct: 79 LNAPPETITFTENVTVGCNIPLWGID--WHPGDHLLLSDCEHQGVIAAAKEIGRRFHLEV 136
Query: 116 TRLGVNHQGRVNPEDLKAA----LRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALF 171
T + +N D A LR NT LV + + G V P+ ++ ++ + + +L
Sbjct: 137 TTCPL--MATLNAGDPVAVVTENLRPNTRLVILSHVLWNTGQVLPLDKIIEVCRDNNSLV 194
Query: 172 HTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRP 214
DA Q G LPL++ L D + + HK + G G G LY++P
Sbjct: 195 LVDAAQSVGLLPLNLTELGADFYAFTGHKWWCGPAGVGGLYVKP 238
>Nos7107_0366 cysteine desulfurase family protein
Length = 455
Score = 59.7 bits (143), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 38/164 (23%), Positives = 73/164 (44%), Gaps = 1/164 (0%)
Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100
R + +AR +A + + ++F + T + R +I++ ++H A
Sbjct: 64 RTDATIASARAAIADFLGCHSDEVVFGANMTTLTFAFSRAIGRELHPGDEIIVTRLDHYA 123
Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159
LE G I + +N ++ +L+ + T LV+V Y + +GT+ IA
Sbjct: 124 NVSSWYALEEQGATIRVVDINVEDCTLDMNELERLINPKTRLVAVTYASNAVGTINDIAA 183
Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
+ ++ GA DAV A P++V +L D L+ S++K +
Sbjct: 184 IARLAHAVGAWVFVDAVHYAPHAPINVHSLDCDFLACSAYKFFA 227
>Nos7107_4671 alanine-glyoxylate aminotransferase apoenzyme
Length = 382
Score = 37.7 bits (86), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 22/80 (27%), Positives = 41/80 (51%), Gaps = 4/80 (5%)
Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+L+ A+ H ++++++ ++ G QP+ +G++ + G L D V G +P+ +
Sbjct: 137 EELRTAMETHRPTILALVHAETSTGARQPLEGVGELCREFGTLLLVDTVTSLGGVPIFLD 196
Query: 188 TLPVDLLSLSSHKLYGGLGA 207
VDL S K GLG
Sbjct: 197 EWGVDLAYSCSQK---GLGC 213
>Nos7107_3892 Isopenicillin-N epimerase
Length = 403
Score = 37.4 bits (85), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 45/205 (21%), Positives = 90/205 (43%), Gaps = 10/205 (4%)
Query: 15 PEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEAD 74
P+A+ Q IL Q ++ +G + +L+ AR ++A INA + ++F T
Sbjct: 37 PKAVLEEQQILRSQLEQ-DPVNFFGRKWEPLLDNARSKLAAFINADIQDLVFVPNATTGV 95
Query: 75 NLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRLGVNHQGRVNPEDLKA 133
N + + ++ ++ ++ E++A + + G ++ + +P+ + A
Sbjct: 96 NSVLRSLT--FSPDDEILTTNHEYNACRNALNFIASSTGAKVVVAKIPFPLE-SPQQIIA 152
Query: 134 ALRH----NTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTL 189
A+ NT L + + S+ G + P+ +L K + G D G + L++Q +
Sbjct: 153 AVLEKVSANTKLALLDHITSQTGLIFPMQQLVKELQARGVDTLIDGAHAPGMISLNIQEI 212
Query: 190 PVDLLSLSSHK-LYGGLGAGALYLR 213
S + HK L GA LY+R
Sbjct: 213 GATYYSGNCHKWLSAPKGAAFLYVR 237
Database: GCA_000316625.1P
Posted date: Aug 27, 2016 10:31 PM
Number of letters in database: 1,703,644
Number of sequences in database: 5237
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5237
Number of Hits to DB: 1,289,620
Number of extensions: 54959
Number of successful extensions: 193
Number of sequences better than 1.0: 10
Number of HSP's gapped: 181
Number of HSP's successfully gapped: 10
Length of query: 382
Length of database: 1,703,644
Length adjustment: 87
Effective length of query: 295
Effective length of database: 1,248,025
Effective search space: 368167375
Effective search space used: 368167375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)