BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000316575.1P
5950 sequences; 1,853,734 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Cal7507_0572 Cysteine desulfurase 636 0.0
Cal7507_0079 cysteine desulfurase NifS 288 1e-93
Cal7507_4946 Cysteine desulfurase 272 5e-88
Cal7507_3103 Cysteine desulfurase 231 3e-72
Cal7507_4264 cysteine desulfurase 83 4e-18
Cal7507_5962 Cysteine desulfurase 67 5e-13
Cal7507_4217 cysteine desulfurase family protein 66 1e-12
Cal7507_0547 alanine-glyoxylate aminotransferase apoenzyme 37 0.002
Cal7507_4702 Glycine dehydrogenase (decarboxylating) 33 0.057
>Cal7507_0572 Cysteine desulfurase
Length = 389
Score = 636 bits (1640), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 313/383 (81%), Positives = 344/383 (89%), Gaps = 1/383 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINA- 59
MQ+YLDYSATTP R EAIA++QT+LTQQWGNPSSLHEWGNRAA I+E AR+QVAGLINA
Sbjct: 1 MQIYLDYSATTPPRSEAIALLQTVLTQQWGNPSSLHEWGNRAATIVEQARIQVAGLINAP 60
Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
VPESIIFTSGGTEADNLAIMGVAR Y+ PQHMIISSVEHSAI EP + LE WGWE+TRLG
Sbjct: 61 VPESIIFTSGGTEADNLAIMGVARLYSAPQHMIISSVEHSAIYEPIRSLERWGWEVTRLG 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
V+ +GRVNP DL+AALR NTVLVSVIYGQSE+GTVQPIAELG I +THG LFHTDAVQ A
Sbjct: 121 VDGKGRVNPLDLQAALRDNTVLVSVIYGQSEVGTVQPIAELGNIARTHGVLFHTDAVQAA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GRLP+DVQ LPVDLLSLSSHKLYG GAGALY+R GVEL PLLGGGGQE GLRSGTQA P
Sbjct: 181 GRLPIDVQQLPVDLLSLSSHKLYGPQGAGALYVRSGVELWPLLGGGGQEMGLRSGTQAVP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
IAGFGVAAELAAQE+ ET RLI LRDR FA LADVPGLIPTGD+ HRLPHH+SF L +
Sbjct: 241 IIAGFGVAAELAAQEMAQETPRLIALRDRAFAQLADVPGLIPTGDRYHRLPHHVSFSLAS 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
ADGEK+SGKTLVR +NLAGIGISAG+AC+SGKLSPSPILLAMGYS++AALGGIRLTLG+
Sbjct: 301 ADGEKLSGKTLVRHMNLAGIGISAGSACHSGKLSPSPILLAMGYSEQAALGGIRLTLGRD 360
Query: 360 TTAADIDWTGIVVKQILQRLIPD 382
TT AD+DWT +V+KQ+LQRLIPD
Sbjct: 361 TTEADVDWTAMVLKQVLQRLIPD 383
>Cal7507_0079 cysteine desulfurase NifS
Length = 402
Score = 288 bits (736), Expect = 1e-93, Method: Compositional matrix adjust.
Identities = 159/378 (42%), Positives = 223/378 (58%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT PE A++ LT+ +GNPSS+H +G + ++ AR +A L+ A
Sbjct: 6 IYLDNNATTKVDPEIAAVMLPYLTEYYGNPSSMHTFGGQLGKDVKLARANIAALLGADES 65
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I + VEH A+ K LE G+ +T L VN
Sbjct: 66 EIVFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQLETQGYTVTYLSVNS 125
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ ++L+A+L NT LV+V+Y +E G + PI ++G K HGALFH DAVQ G++
Sbjct: 126 QGQLDLDELEASLTGNTALVTVMYANNETGVIFPIEQIGLRVKEHGALFHVDAVQAVGKI 185
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T VD+L++S HK++ G GALY+R GV P L GG QE G R+GT+ P I
Sbjct: 186 PLNMKTSTVDMLTISGHKIHAPKGIGALYIRRGVRFRPFLIGGHQERGRRAGTENVPGII 245
Query: 243 GFGVAAELAAQELETETGRLILLRDRL-FAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAEL LE T R LRDRL +LA +P GD RLP+ + + +
Sbjct: 246 ALGKAAELEMLHLEEATKREARLRDRLEKTLLATIPDCEVNGDPTQRLPNTTNIGFKYIE 305
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC SG L PS +L +MG G IR +L + TT
Sbjct: 306 GEAI-----LLSLNKYGICASSGSACTSGSLEPSHVLRSMGLPYTTLHGSIRFSLSRYTT 360
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ +I++RL
Sbjct: 361 DAEIDQVIAVMPEIVERL 378
>Cal7507_4946 Cysteine desulfurase
Length = 388
Score = 272 bits (696), Expect = 5e-88, Method: Compositional matrix adjust.
Identities = 154/370 (41%), Positives = 225/370 (60%), Gaps = 13/370 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNP-SSLHEWGNRAALILETARMQVAGLINAVP 61
+YLD ATT +A + T+ +GNP S+ H +G A ++ AR +A INA P
Sbjct: 6 IYLDNHATTAVDKRVLAAMLPYFTENFGNPASNSHVYGWEAEAAIKQAREILATAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y QH+I ++ EH A+ +P + L+ G++IT L V
Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFQKGQHIITAATEHKAVLDPCEYLKTLGFDITILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ +L+ ALR +T+LVSV+ +EIG +QP+ E+G+I + LFHTDA Q G
Sbjct: 126 QKDGLIDLTELEKALRPDTILVSVMAANNEIGVLQPLKEIGEICRHRQILFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV++ +DL+SL++HK+YG G GALY+R P V++ P GGG E G+RSGT
Sbjct: 186 KIPLDVRSQKIDLMSLTAHKVYGPKGIGALYVRRRNPRVQIAPQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A E+A E TE RL LR+RL+ L+ + G+ G RL +L+ +
Sbjct: 246 TPQIVGFGKAVEIALTEQATENQRLTSLRERLWLHLSHLQGIQINGHPTQRLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L + +S+G+AC S +PS +L A+G+S++ A +R +
Sbjct: 306 EGVDGAAL-------LLGLQPVAAVSSGSACTSTNTAPSHVLTALGHSEQLAYASVRFGI 358
Query: 357 GKQTTAADID 366
G+ T +ID
Sbjct: 359 GRLNTEEEID 368
>Cal7507_3103 Cysteine desulfurase
Length = 390
Score = 231 bits (590), Expect = 3e-72, Method: Compositional matrix adjust.
Identities = 140/376 (37%), Positives = 205/376 (54%), Gaps = 19/376 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY +TTP P + +T +GN +S+ H +G+ AA ++ AR Q+AGLINA P
Sbjct: 5 IYLDYHSTTPVDPRVAEKVMYYMTTDFGNANSVDHGYGDTAAQAVKKARQQIAGLINASP 64
Query: 62 ESIIFTSGGTEADNLAIMG---------VARCYTTPQHMIISSVEHSAISEPAKMLENWG 112
+ IIFTSG TE+ NL I G + R TP +I+S VEH A+ + K L G
Sbjct: 65 KEIIFTSGATESINLVIQGQISTSLDYHIVR-QNTPAKIIVSPVEHKAVLDTCKALAKKG 123
Query: 113 W-EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALF 171
EI L VN Q +++ E L+ L+ V+ +E+GT+ PI ++G I +H F
Sbjct: 124 LAEIIWLKVNQQAQIDLEHLEKVCSDGAALLCVMAANNEVGTIYPIKKIGAIASSHNIPF 183
Query: 172 HTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGL 231
DA Q AG++PL+ Q + L++S HK Y G GA +R G ++ GGG E GL
Sbjct: 184 LCDASQAAGKIPLNFQDWGITYLAISGHKFYAPKGVGAFVVRNGYSPQAMIYGGGHEQGL 243
Query: 232 RSGTQATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRLP 290
RSGT P I G G A L E+ET+ + LR++ +L + +P L+ GD +RL
Sbjct: 244 RSGTLNVPGIVGLGEACRLRGLEMETDENAIAFLRNQFQTLLQEQIPDLVVNGDLNNRLS 303
Query: 291 HHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALG 350
+L + I + VR + IS G+AC+SG ++PS +L AM S+ G
Sbjct: 304 GNLHISIPDIPNSAIIAR--VRH----QLAISTGSACSSGVVAPSHVLQAMHLSENIIEG 357
Query: 351 GIRLTLGKQTTAADID 366
+R+ +GK TT A+I+
Sbjct: 358 ALRMGVGKFTTKAEIE 373
>Cal7507_4264 cysteine desulfurase
Length = 420
Score = 82.8 bits (203), Expect = 4e-18, Method: Compositional matrix adjust.
Identities = 67/233 (28%), Positives = 113/233 (48%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
VYLD +AT+ +P + ++ T+ +++H + +A E AR ++A LIN
Sbjct: 30 VYLDNAATS-QKP--LFVLNTLRDYYEHYNANVHRGAHTLSAKATDAYEGARDKIAKLIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T +EA NL P +I+S +EH + P + + + G +
Sbjct: 87 AASRQEIVYTRNASEAINLVAYSWGMHNLQPGDEIILSVMEHHSNIVPWQFVAQKTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + E K + T LVSV++ + +G + P+ E+ I +GA F DA
Sbjct: 147 KFVELTPEETFDLEQFKQLISDKTKLVSVVHVSNTLGCINPVQEIATIAHRYGAKFLVDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR-PGVELMPLLGGGGQ 227
Q +P+DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 207 CQSVPHMPVDVQQIDCDWLVASGHKMCAPTGIGFLYGKLELLESMPPFFGGGE 259
>Cal7507_5962 Cysteine desulfurase
Length = 394
Score = 67.4 bits (163), Expect = 5e-13, Method: Compositional matrix adjust.
Identities = 60/218 (27%), Positives = 102/218 (46%), Gaps = 8/218 (3%)
Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQ-QWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
++Y +Y P + +I T Q P S E G+ ++T R +A ++
Sbjct: 24 KIYFNYGGQGPMPQRVLDVIAQSQTHIQHIGPFST-EVGHWIDEEIKTTREAIASELHVS 82
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHS-AISEPAKMLENWGWEITR-- 117
P++I FT T N+ + G+ + H+++S EH I+ ++ + E+T
Sbjct: 83 PQTITFTENVTVGCNIGLWGID--WQAGDHLLLSDCEHPGVIATTQEIARRFAVEVTTCP 140
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
L V + + + LR NT LV + + G V P+ ++ +I K + +L DA Q
Sbjct: 141 LMVTLNEGDSVKAIAQHLRPNTRLVVLSHVLWNTGQVLPLDKIVEICKNNNSLLLVDAAQ 200
Query: 178 VAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
G LPL++ L VD + + HK L G G G LY++P
Sbjct: 201 SVGALPLNLAELGVDFYAFTGHKWLCGPAGVGGLYVKP 238
>Cal7507_4217 cysteine desulfurase family protein
Length = 457
Score = 66.2 bits (160), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 40/164 (24%), Positives = 77/164 (46%), Gaps = 1/164 (0%)
Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100
R +++ AR A + + ++F + T +AR +I++ ++H A
Sbjct: 64 RTDALIDAARAASADFLGCDSDEVVFGANMTTLTFTLSRAIARELKPGDEIIVTRLDHYA 123
Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159
P LE G I + +N ++ ++L+ + T LV++ Y + +GT+ +A+
Sbjct: 124 NVSPWYALEEQGVTIRVVDINVEDCTLDMQELEQQINQRTRLVAIGYASNAVGTINDVAQ 183
Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
+ + GAL DAV P+DV++L D L+ S +K +G
Sbjct: 184 VVLLAHAVGALVFVDAVHYTPHAPIDVRSLDCDFLACSVYKFFG 227
>Cal7507_0547 alanine-glyoxylate aminotransferase apoenzyme
Length = 380
Score = 37.4 bits (85), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 22/83 (26%), Positives = 42/83 (50%), Gaps = 4/83 (4%)
Query: 126 VNPEDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPL 184
N ++L+ A+ H ++++++ ++ G QP+ +G++ + G L D V G +P+
Sbjct: 134 FNLDELRTAIATHRPTILALVHAETSTGARQPLEGVGELCREFGTLLLVDTVTSLGGVPI 193
Query: 185 DVQTLPVDLLSLSSHKLYGGLGA 207
+ VDL S K GLG
Sbjct: 194 FLDNWGVDLAYSCSQK---GLGC 213
>Cal7507_4702 Glycine dehydrogenase (decarboxylating)
Length = 980
Score = 32.7 bits (73), Expect = 0.057, Method: Compositional matrix adjust.
Identities = 35/138 (25%), Positives = 60/138 (43%), Gaps = 20/138 (14%)
Query: 112 GWEITRLGVNHQGRVNPEDLKA-ALRHNTVLVS--VIYGQSEIGTVQPIAELGKITKTHG 168
G ++ + + QG ++ +DLKA A +H+ L + V Y + + I E+ + THG
Sbjct: 624 GMKVVAIACDDQGNIDVDDLKAKAQKHSHELAALMVTYPSTHGVFEEAIQEICAVVHTHG 683
Query: 169 ALFHTDAVQVAGRL----PLDVQTLPVDLLSLSSHKLYG----------GLGAGALYLRP 214
+ D + ++ P D+ D+ L+ HK + G A +L P
Sbjct: 684 GQVYMDGANMNAQVGICRPGDIG---ADVCHLNLHKTFCIPHGGGGPGMGPIGVAAHLVP 740
Query: 215 GVELMPLLGGGGQENGLR 232
+ P+LG G E G R
Sbjct: 741 FLPGHPVLGTGDWELGTR 758
Database: GCA_000316575.1P
Posted date: Aug 27, 2016 10:54 PM
Number of letters in database: 1,853,734
Number of sequences in database: 5950
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5950
Number of Hits to DB: 1,408,221
Number of extensions: 59524
Number of successful extensions: 208
Number of sequences better than 1.0: 10
Number of HSP's gapped: 196
Number of HSP's successfully gapped: 10
Length of query: 382
Length of database: 1,853,734
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,330,134
Effective search space: 391059396
Effective search space used: 391059396
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)