BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000316115.1P
7828 sequences; 2,521,536 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Lepto7375DRAFT_4103 cysteine desulfurase family protein 510 0.0
Lepto7375DRAFT_2148 cysteine desulfurase NifS 250 2e-79
Lepto7375DRAFT_5569 cysteine desulfurase family protein 246 5e-78
Lepto7375DRAFT_3255 cysteine desulfurase-like protein, SufS subf... 86 5e-19
Lepto7375DRAFT_1231 cysteine desulfurase family protein, VC1184 ... 66 2e-12
Lepto7375DRAFT_3829 selenocysteine lyase 56 2e-09
Lepto7375DRAFT_3365 selenocysteine lyase 47 2e-06
Lepto7375DRAFT_4236 serine-pyruvate aminotransferase/archaeal as... 35 0.014
Lepto7375DRAFT_5152 PLP-dependent enzyme, glutamate decarboxylase 33 0.044
Lepto7375DRAFT_6010 nucleoside-diphosphate-sugar epimerase 30 0.48
>Lepto7375DRAFT_4103 cysteine desulfurase family protein
Length = 394
Score = 510 bits (1314), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 252/384 (65%), Positives = 305/384 (79%), Gaps = 4/384 (1%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTP R E +A + + + WGNPSSLH WG+RAA ++ETARMQVA L+ A
Sbjct: 1 MQIYLDYSATTPPRAEVMAAMGSAMKHSWGNPSSLHNWGSRAATVMETARMQVADLMGAS 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
++++FT+ GTEA+NLAIMGVA+ Y P+HMIIS+VEHSAI++P +LE WGW+ITRL V
Sbjct: 61 GDAVVFTASGTEANNLAIMGVAQQYRKPRHMIISAVEHSAIAKPITLLEQWGWQITRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHG----ALFHTDAV 176
+ G+V+P DL ALR +TVLVSVIYGQSE+GT+QPI EL + K LFH+DAV
Sbjct: 121 DSYGQVHPIDLAQALREDTVLVSVIYGQSEVGTIQPITELAAVIKASDHISTPLFHSDAV 180
Query: 177 QVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQ 236
QVAGRLP+DVQ L VDLLS+SSHKLYG GAGALY++PG +LMPL+ GGGQEN LRSGTQ
Sbjct: 181 QVAGRLPIDVQKLGVDLLSISSHKLYGPQGAGALYVKPGTDLMPLINGGGQENHLRSGTQ 240
Query: 237 ATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFY 296
A P I GFGVAAELA QEL E RL+ LRDRL L P L+ TG RLPHH+SFY
Sbjct: 241 AVPTIVGFGVAAELARQELSQEQQRLLKLRDRLIEQLQCHPELLLTGHPQQRLPHHVSFY 300
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
L A+GE++SGKTLVRQLNLAGIGISAG+AC+SGKL+PSPIL AMG+S AA GIRLTL
Sbjct: 301 LPNANGEQVSGKTLVRQLNLAGIGISAGSACHSGKLTPSPILQAMGFSNIAAKTGIRLTL 360
Query: 357 GKQTTAADIDWTGIVVKQILQRLI 380
G QTT AD+DWT +VV+QI++R++
Sbjct: 361 GHQTTEADVDWTAMVVRQIIERVM 384
>Lepto7375DRAFT_2148 cysteine desulfurase NifS
Length = 397
Score = 250 bits (639), Expect = 2e-79, Method: Compositional matrix adjust.
Identities = 147/380 (38%), Positives = 215/380 (56%), Gaps = 7/380 (1%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
M +YLD +ATT P+ + + L+ +GNPSS+H +G + ++ AR++VA L+ A
Sbjct: 1 MVIYLDNNATTRVDPDVLDAMLPYLSDLYGNPSSMHSFGGQVGAAIQDARVRVAELLGAE 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
IIFTS G+E +N AI +H++ ++VEH AI K LE G+ +T L V
Sbjct: 61 DTEIIFTSCGSEGNNTAIHAALAVQPEKRHIVTTAVEHPAILNVCKHLEKKGYTVTYLSV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ +G+++ +++AA+ T LV+ +Y +E G + PI ++G I K GA FH DAVQ G
Sbjct: 121 DRRGQLDLMEVEAAMTGGTALVTTMYANNETGVIFPIEQIGAIAKECGATFHVDAVQAVG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
+LP+D++ +DLL+LS HKL+ G GALY+R G P L GG QE G R+GT P
Sbjct: 181 KLPIDLKNSTIDLLTLSGHKLHAPKGVGALYVRRGFRFRPFLLGGHQERGRRAGTHNVPG 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRLPHHLSFYLEA 299
I G AAE+A + L R LRD+L + L + +P G RLP+ + +
Sbjct: 241 IIALGKAAEVAREHL-AAVHRERQLRDQLESQLLELIPDCDVNGGGTPRLPNTTNIGFKY 299
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
+GE I + LN GI S+G+AC SG L PS +L AMG G IR +L +
Sbjct: 300 IEGEAI-----LFMLNQHGICASSGSACTSGSLDPSHVLTAMGLPYTILHGSIRFSLSRF 354
Query: 360 TTAADIDWTGIVVKQILQRL 379
TTA DI V+ I+++L
Sbjct: 355 TTAEDIQQVVNVMPGIVEKL 374
>Lepto7375DRAFT_5569 cysteine desulfurase family protein
Length = 383
Score = 246 bits (629), Expect = 5e-78, Method: Compositional matrix adjust.
Identities = 153/381 (40%), Positives = 223/381 (58%), Gaps = 10/381 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD ATTP + ++ T+ +GNPSS+H +G ++ +R ++ INA PE
Sbjct: 6 IYLDCHATTPVDQRVVEVMLPFFTEHFGNPSSVHHYGWPVEAAIKKSRETISHSINAQPE 65
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
I+FTSG TEA+NLAI GVA Y + +H+I EHSA+ +P + LE+ G+E+T L V
Sbjct: 66 EIVFTSGATEANNLAIKGVAEAYFSHGKHIITVQTEHSAVLDPCRYLESLGFEVTYLPVE 125
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
G ++ L+ A+R T+LVSV+ +EIG +QP+ E+G + + LFHTDA Q G+
Sbjct: 126 PDGLLSLPLLEKAIRPETILVSVMAANNEIGVLQPLKEIGALCQEREILFHTDAAQAIGK 185
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQAT 238
+PLDVQ + + L+S+++HK+YG G GALY+R P V L L GGG E G+RSGT
Sbjct: 186 VPLDVQAMNIHLMSMTAHKVYGPKGIGALYVRRRHPRVNLAAQLHGGGHERGMRSGTLYP 245
Query: 239 PAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLE 298
P I GF A ELA E+ E RL LR +L L +P G RLP +L+
Sbjct: 246 PQIVGFAKALELATAEMAVERERLTGLRSQLLTHLHQLPDSYLNGHPTQRLPGNLNMSFG 305
Query: 299 AADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGK 358
DG+ +L+ L + + +S+G+AC+S +PS +L A+G S + A +R LG+
Sbjct: 306 GVDGQ-----SLLLGLQ-SVVALSSGSACSSASTAPSHVLKALGRSDQLAYASLRFGLGR 359
Query: 359 QTTAADIDWTGIVVKQILQRL 379
TT +I G V + +Q L
Sbjct: 360 FTTEQEIHQVGQTVVETVQAL 380
>Lepto7375DRAFT_3255 cysteine desulfurase-like protein, SufS subfamily
Length = 420
Score = 85.9 bits (211), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 101/366 (27%), Positives = 153/366 (41%), Gaps = 62/366 (16%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
VY D +AT+ +P+ + A++ +H RA E R +VA INA
Sbjct: 30 VYFDNAATS-QKPKVVLNALVDYYHQDNANVHRGVHTLSARATAAYEGTRDKVAAFINAS 88
Query: 61 PES-IIFTSGGTEADNLAIM--GVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEIT 116
+ I+FT +EA NL G++ + +I+S +EH + P +++ E G +
Sbjct: 89 SRNEIVFTRNASEAINLVAYAWGLSNLKAGDE-VILSVMEHHSNLVPWQLVAERTGAVLK 147
Query: 117 RLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAV 176
+ + + E K L T LV++ + + +G + P+ ++ K+ GA DA
Sbjct: 148 HVQLTDTQEFDFEHFKELLCDRTRLVAINHISNTLGCINPVEDVIKLAHGQGAKVLIDAC 207
Query: 177 QVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG--QENGLR 232
Q +P+DVQ+L D L S HK+ G GAG LY + + + P LGGG Q+ L
Sbjct: 208 QSVPHMPVDVQSLDCDWLVASGHKMCGPTGAGFLYGKLDILKAMPPFLGGGEMIQDVYLD 267
Query: 233 SGTQA---------TPAIAGF-----------GVAAELAAQELETETGRLILLRDRLFAI 272
T A TPAIA G+ + AQ E E R + R
Sbjct: 268 HSTYAELPHKFEAGTPAIAEVIMLGAAVDYLTGIGMDRIAQ-YEHELTRYLYER------ 320
Query: 273 LADVPGLIPTGDKIHRLPHHLSFYLEAADG-----------EKISGKTLVRQLNLAGIGI 321
LADVP + G K ADG E + + L L+ GI I
Sbjct: 321 LADVPKIKLYGPK------------PKADGSGRAAIATFTVENLHAQDLSTFLDQTGIAI 368
Query: 322 SAGAAC 327
+G C
Sbjct: 369 RSGHHC 374
>Lepto7375DRAFT_1231 cysteine desulfurase family protein, VC1184 subfamily
Length = 427
Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 62/278 (22%), Positives = 114/278 (41%), Gaps = 30/278 (10%)
Query: 25 LTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARC 84
LT N R ++ AR +A + P I+F + T +
Sbjct: 59 LTHSNANAHGAFATSQRTDEVIAAARGAMADFLGCNPNEIVFGANMTSLTFAISRAIGHA 118
Query: 85 YTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQG-RVNPEDLKAALRHNTVLVS 143
++++ ++H A P L G + + ++ + ++ +DL + L H T L++
Sbjct: 119 LLPGDEILVTCLDHDANIAPWLALREQGIIVHTVDIHPENCTLDLKDLISKLNHRTRLLA 178
Query: 144 VIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
+ Y + +GT+ + + ++ GA + DAV A P+DVQ L D L S +K +G
Sbjct: 179 ITYASNAVGTLNDLPTIIQLAHNAGAWVYIDAVHYAAHGPIDVQQLDCDFLVCSPYKFFG 238
Query: 204 GLGAGALY-------------LRPGVELMPLLGGGGQENGLRSGTQATPAIAGFGVAAEL 250
G LY +RP +++P +GTQ +AG A E
Sbjct: 239 P-HMGVLYGKRDHLERLHPYKVRPASDVIPY--------RWETGTQNHEGLAGVTAAIEY 289
Query: 251 AAQELETETGRLI--LLRDRLFAILADVPGLIPTGDKI 286
A + GRL+ + R A+ A + + +G+++
Sbjct: 290 LA-----DLGRLVSPQMSKRRGAVFAAMTAIRHSGEQL 322
>Lepto7375DRAFT_3829 selenocysteine lyase
Length = 398
Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 60/233 (25%), Positives = 99/233 (42%), Gaps = 18/233 (7%)
Query: 4 YLDYSATTPTRPEAIAIIQTI-LTQQWGNP--SSLHEWGNRAALILETARMQVAGLINAV 60
Y +Y P A+ IQ T Q P S+++ W E R +A +
Sbjct: 20 YFNYGGQGPMVLNALKAIQFAHQTMQTRGPFGSAVNTWMQVEG---EKTRRVLADELGVS 76
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P++++ T T N+A+ G++ + H+++S EH I L+ +++
Sbjct: 77 PQTMVLTEDVTVGCNIALWGMS--WRNGDHILLSDCEHPGIFAAVYELQR-RFDLEVSIF 133
Query: 121 NHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFH--TD 174
H ++N + +++ LR NT LV V + G V P+ E+ ++ +D
Sbjct: 134 PHLSQINMANPVAMIQSQLRPNTRLVVVSHVLWNTGQVMPLTEICQLCHAQPQTVRVLSD 193
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGV--ELMPLLGG 224
A Q G LPL + VD + + HK G G G LY+ P EL P G
Sbjct: 194 AAQSVGMLPLQMAETGVDFYAFTGHKWCCGPAGLGGLYVSPAAMEELAPTFIG 246
>Lepto7375DRAFT_3365 selenocysteine lyase
Length = 343
Score = 46.6 bits (109), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 42/161 (26%), Positives = 72/161 (44%), Gaps = 5/161 (3%)
Query: 46 LETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVA--RCYTTPQHMIISSVEHSAISE 103
++ A Q+A +N + + F G + NL ++ A + +I+S+ +H A +
Sbjct: 1 MDEAYCQLAAYLNVSEDDVHF--GPSTTQNLYVLAHAFREIWNEGDEIILSNQDHEANAG 58
Query: 104 PAKMLENWGWEITRLGVNHQ-GRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGK 162
+ LE G I + + GR+NP+ L L T L++ + + IG P+AE+
Sbjct: 59 FWRRLEKTGIVIKEWRIAPETGRLNPDHLDTLLSDRTRLLAFPHCSNVIGHTNPVAEIVS 118
Query: 163 ITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
KT D V A D+ L VD+ S +K +G
Sbjct: 119 KVKTADICVVVDGVAHAPHGLPDIGALGVDIYLFSLYKTWG 159
>Lepto7375DRAFT_4236 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 386
Score = 35.0 bits (79), Expect = 0.014, Method: Compositional matrix adjust.
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Query: 130 DLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQT 188
+L+AAL H ++++++ ++ G QP+ +G + + L D V G +P+ +
Sbjct: 141 ELRAALEEHRPAILALVHAETSTGARQPLEGVGDLCREFDCLLLVDTVTSLGGVPIFLDE 200
Query: 189 LPVDLLSLSSHK 200
VD+ S K
Sbjct: 201 WKVDMAYSCSQK 212
>Lepto7375DRAFT_5152 PLP-dependent enzyme, glutamate decarboxylase
Length = 482
Score = 33.5 bits (75), Expect = 0.044, Method: Compositional matrix adjust.
Identities = 39/143 (27%), Positives = 59/143 (41%), Gaps = 21/143 (14%)
Query: 52 QVAGLINAVPESI-IFTSGGTEADNLAIMGVAR--CYTTPQH---------MIISSVEHS 99
QVA L P++ + SGG+ A NL + VAR + H +S V H+
Sbjct: 134 QVAQLFGLGPQANGVLVSGGSLA-NLQALIVARNAAFNALDHGITQVKQPVFFVSEVAHT 192
Query: 100 AISEPAKMLENWGWEITRLGVNHQG-RVNPEDLKAALRH------NTVLVSVIYGQSEIG 152
+ + A M+ G + HQ +++ DLK L + G + G
Sbjct: 193 SFKK-AAMVMGLGTKAAIPVATHQNSQIDITDLKTKLTQAKANGQQPFAIVATAGTTVTG 251
Query: 153 TVQPIAELGKITKTHGALFHTDA 175
++ PIAE+ I H FH DA
Sbjct: 252 SIDPIAEMAHIANLHQLWFHVDA 274
>Lepto7375DRAFT_6010 nucleoside-diphosphate-sugar epimerase
Length = 300
Score = 30.0 bits (66), Expect = 0.48, Method: Compositional matrix adjust.
Identities = 21/51 (41%), Positives = 26/51 (50%), Gaps = 9/51 (17%)
Query: 112 GWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQS--EIGTVQPIAEL 160
G E+ R R +PEDLKAAL T IY + E+G QP+ EL
Sbjct: 35 GVEVVRCD-----RKSPEDLKAALAGKT--FDAIYDNNGRELGDTQPLVEL 78
Database: GCA_000316115.1P
Posted date: Aug 27, 2016 11:21 PM
Number of letters in database: 2,521,536
Number of sequences in database: 7828
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 7828
Number of Hits to DB: 1,935,994
Number of extensions: 82646
Number of successful extensions: 261
Number of sequences better than 1.0: 13
Number of HSP's gapped: 255
Number of HSP's successfully gapped: 13
Length of query: 382
Length of database: 2,521,536
Length adjustment: 90
Effective length of query: 292
Effective length of database: 1,817,016
Effective search space: 530568672
Effective search space used: 530568672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)