BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000312725.1P
3994 sequences; 1,117,529 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MICAE_2260001 iscS Cysteine desulfurase 538 0.0
MICAE_70002 iscS Cysteine desulfurase 246 6e-78
MICAE_1090009 Cysteine desulfurase (fragment) 100 1e-26
MICAE_1520038 sufS cysteine desulfurase 89 2e-20
MICAE_460002 L-cysteine/cystine lyase 56 2e-09
MICAE_410083 Cysteine desulfurase like 37 0.001
>MICAE_2260001 iscS Cysteine desulfurase
Length = 388
Score = 538 bits (1386), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 259/380 (68%), Positives = 309/380 (81%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR QVAGLINA
Sbjct: 1 MQIYLDYSATTPTHPQVIDRVATILQHHWGNPSSLHSWGQDTATVIEMAREQVAGLINAN 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P+ IIFTSGGTEADNLAI+GVA+ Y P+H+IISSVEHSAI+EP K LE GW+ITRL V
Sbjct: 61 PDQIIFTSGGTEADNLAIIGVAQQYDRPRHIIISSVEHSAIAEPCKQLEQQGWQITRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N QGRVNP DLKAA++ +TVL+S+IYGQSE+GT+QPI ELG I + G LFHTDAVQVA
Sbjct: 121 NRQGRVNPLDLKAAIQSDTVLISIIYGQSEVGTLQPIEELGSIARKRGVLFHTDAVQVAA 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R +DV+ LPVDLLSLSSHK+YG G+GALY+R GV+++PLL GGGQE GLRSGT A PA
Sbjct: 181 RCDIDVRKLPVDLLSLSSHKIYGMQGSGALYVRAGVDILPLLRGGGQEKGLRSGTPAVPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IA FG+AAELA ++L +E RLI LRDRLF +LAD P L+PTGD+ +RLPHH+SF +
Sbjct: 241 IAAFGLAAELAQKDLISEKMRLIALRDRLFDLLADYPYLLPTGDRFYRLPHHVSFIVRPD 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
D I+GK LVRQLNLAGIGIS+G+AC+SGKLSPSPIL AMGYS + AL GIRLTLG+ T
Sbjct: 301 DNSHITGKQLVRQLNLAGIGISSGSACHSGKLSPSPILKAMGYSDREALAGIRLTLGRDT 360
Query: 361 TAADIDWTGIVVKQILQRLI 380
+AADIDWT +V+KQ++ R +
Sbjct: 361 SAADIDWTALVLKQVIDRCL 380
>MICAE_70002 iscS Cysteine desulfurase
Length = 387
Score = 246 bits (627), Expect = 6e-78, Method: Compositional matrix adjust.
Identities = 149/373 (39%), Positives = 222/373 (59%), Gaps = 13/373 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P+ +A + T+ +GN SS+ H +G A ++ AR +A IN+ P
Sbjct: 6 IYLDCHATTPLDPQVLAAMLPYFTEHFGNASSINHVYGWTAEAAVKQARETIAAAINSSP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLAI GVA Y +H++ EH A+ +P LE G+E+T L V
Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFAKGRHIVTVMTEHRAVLDPCHYLEKLGFEVTILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ G ++ E L+ +LR +T+L+S++ +EIG +QP+A +G I + + LFHTDA Q G
Sbjct: 126 SGDGLIDLELLEKSLRPDTILLSIMAANNEIGVIQPLAAIGAICRQYQVLFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK++G G GALY+R P V L + GGGQE GLRSGT
Sbjct: 186 KIPLDVEEMNIDLMSLTAHKVHGPKGIGALYVRRRNPRVRLAAQIQGGGQEKGLRSGTIF 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A EL + +E +T L L+ RL+ I++ + + G RL +L+ +
Sbjct: 246 TPQIVGFAKAVELGIKAIEEDTSHLNQLKARLWEIISQLDRIYLNGHPSQRLAGNLNISI 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L I +S+G+AC+S +PS +L A+G + A +R L
Sbjct: 306 EGVDGAAL-------LLGLQPIVALSSGSACSSSHTAPSHVLTALGRPESLAYASLRFGL 358
Query: 357 GKQTTAADIDWTG 369
+ T +I+ G
Sbjct: 359 SRFNTLEEIELVG 371
>MICAE_1090009 Cysteine desulfurase (fragment)
Length = 94
Score = 99.8 bits (247), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 46/71 (64%), Positives = 53/71 (74%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL W NPSSLH WG A ++E AR QVAGLI+A
Sbjct: 1 MQIYLDYSATTPTHPQVIERVVTILQHHWDNPSSLHSWGQDTATVIEMAREQVAGLIHAN 60
Query: 61 PESIIFTSGGT 71
P+ IIFTSGGT
Sbjct: 61 PDQIIFTSGGT 71
>MICAE_1520038 sufS cysteine desulfurase
Length = 420
Score = 89.4 bits (220), Expect = 2e-20, Method: Compositional matrix adjust.
Identities = 71/233 (30%), Positives = 115/233 (49%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
+YLD SA T +P I +++T+ + +++H + RA E AR +VA IN
Sbjct: 30 IYLD-SAATSQKP--IQVLETLRNYYEKDNANVHRGAHTLSVRATEAYEGARDKVARFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T TEA NL A +I+S +EH + P +++ + G I
Sbjct: 87 AASRQEIVYTRNATEAINLVAYSWALNTLKAGDEIILSVMEHHSNLIPWQIVCQKTGAVI 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + E K+ L T LV+V++ + +G + P+AE+G + GA DA
Sbjct: 147 KYVQLTSEESFDLEQYKSLLSDKTKLVAVLHVSNTLGCINPVAEIGSLAHQVGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227
Q L +DVQ++ D L S HK+ G G LY + + E MP GGG+
Sbjct: 207 CQSVPHLAIDVQSIDCDWLVASGHKMCAPTGIGFLYGKEAILEAMPPFFGGGE 259
>MICAE_460002 L-cysteine/cystine lyase
Length = 404
Score = 55.8 bits (133), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 50/185 (27%), Positives = 83/185 (44%), Gaps = 13/185 (7%)
Query: 47 ETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAK 106
E R ++A + P ++ T T N+A+ GV + +H++++ EH I +
Sbjct: 76 ELLRQEMAQELGISPSNLSITEDVTVGCNIALWGVD--WQAGEHILLTDCEHPGIMATVR 133
Query: 107 ML-ENWGWEITRLGVNHQ-GRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKI 163
+ + EI+ + NP E + A LR T ++ V + G V P+ E+ ++
Sbjct: 134 EIARRYHLEISTCPIRETLNGGNPIEVISAHLRPKTRVLVVSHVLWNTGQVLPLKEISQL 193
Query: 164 TKTHGA-----LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGVE 217
+ L DA Q G LPLD+ D + + HK + G G G LY+RP E
Sbjct: 194 CHDNSVTEKPVLVVVDAAQSVGCLPLDLSATAADCYAFTGHKWWCGPAGVGGLYIRP--E 251
Query: 218 LMPLL 222
+ P L
Sbjct: 252 IFPSL 256
>MICAE_410083 Cysteine desulfurase like
Length = 386
Score = 37.0 bits (84), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 37/174 (21%), Positives = 77/174 (44%), Gaps = 7/174 (4%)
Query: 45 ILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
+L+ AR ++A L+ + ++F T A N + + + + ++I+ ++A +
Sbjct: 53 LLDIARQKLADLVGVKSDDLVFVPNATTAVNAVLNSLT--FQENEEILITDQTYNACANA 110
Query: 105 AKML-ENWGWE--ITRLGVNHQGRVN-PEDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
K + + WG + I ++ Q + + + A++ T LV + + S + PI E+
Sbjct: 111 VKHIAKRWGLKVIIAKIPFPVQSPLEISQAILASVSPRTKLVVLDHVTSPTALIWPITEI 170
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213
+ G D G LPL++ + + + HK L G+ LY+R
Sbjct: 171 VQELNNRGIDTLIDGAHALGFLPLNIGAINPTYYTANCHKWLCSPKGSAFLYVR 224
Database: GCA_000312725.1P
Posted date: Aug 27, 2016 10:38 PM
Number of letters in database: 1,117,529
Number of sequences in database: 3994
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3994
Number of Hits to DB: 844,171
Number of extensions: 35390
Number of successful extensions: 114
Number of sequences better than 1.0: 7
Number of HSP's gapped: 109
Number of HSP's successfully gapped: 7
Length of query: 382
Length of database: 1,117,529
Length adjustment: 84
Effective length of query: 298
Effective length of database: 782,033
Effective search space: 233045834
Effective search space used: 233045834
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)