cyanobase / GCA_001277295.1 - AA650

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000312285.1P 4483 sequences; 1,237,661 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MICAK_510001 iscS Cysteine desulfurase 536 0.0 MICAK_1080011 NIFS Cysteine desulfurase 1, mitochondrial 243 5e-77 MICAK_670002 sufS cysteine desulfurase 84 2e-18 MICAK_2800004 L-cysteine/cystine lyase 57 5e-10 MICAK_170013 Cysteine desulfurase like 43 2e-05 6a24524cae892bfcd3567c09e3da0257

>MICAK_510001 iscS Cysteine desulfurase Length = 388 Score = 536 bits (1380), Expect = 0.0, Method: Compositional matrix adjust. Identities = 258/380 (67%), Positives = 309/380 (81%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR +VAGLINA Sbjct: 1 MQIYLDYSATTPTHPQVIERVATILRHHWGNPSSLHTWGQDTATVIEMAREEVAGLINAN 60 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 P+ IIFTSGGTEADNLAI+GVA+ Y P+H+IISSVEHSAI+EP K LE GW+ITRL V Sbjct: 61 PDQIIFTSGGTEADNLAIIGVAQQYNRPRHIIISSVEHSAIAEPCKQLEQQGWQITRLPV 120 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 N QGRVNP DLKAA++ +TVL+S+IYGQSE+GT+QPI ELG I + G LFHTDAVQVA Sbjct: 121 NRQGRVNPLDLKAAIQSDTVLISIIYGQSEVGTLQPIEELGSIARERGVLFHTDAVQVAA 180 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240 R +DV+ LPVDLLSLSSHK+YG G+GALY+R GV+++PLL GGGQE GLRSGT A PA Sbjct: 181 RCDIDVRKLPVDLLSLSSHKIYGMQGSGALYIRAGVDILPLLRGGGQEKGLRSGTPAVPA 240 Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300 IA FG+AAELA ++L +E RLI LRDRLF +LAD P L+PTGD+ +RLPHH+SF + Sbjct: 241 IAAFGLAAELAQKDLISEKMRLIALRDRLFDLLADYPYLLPTGDRFYRLPHHVSFIVRPD 300 Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360 D I+GK LVRQLNLAGIGIS+G+AC+SGKLSPSPIL AMGYS + AL GIRLTLG+ T Sbjct: 301 DDSHITGKQLVRQLNLAGIGISSGSACHSGKLSPSPILKAMGYSDREALAGIRLTLGRDT 360 Query: 361 TAADIDWTGIVVKQILQRLI 380 +AADIDWT +V+KQ++ R + Sbjct: 361 SAADIDWTALVLKQVIDRCL 380 >MICAK_1080011 NIFS Cysteine desulfurase 1, mitochondrial Length = 387 Score = 243 bits (621), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 148/373 (39%), Positives = 221/373 (59%), Gaps = 13/373 (3%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLD ATTP P+ +A + T+ +GN SS+ H +G A ++ AR +A IN+ P Sbjct: 6 IYLDCHATTPMEPQVLAAMLPYFTEHFGNASSINHVYGWTAEAAVKQARETIAAAINSSP 65 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E IIFTSG TEA+NLAI GVA Y +H++ EH A+ +P LE G+E+T L V Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFAKGRHIVTVVTEHRAVLDPCHYLEKLGFEVTILPV 125 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 + G ++ E L+ +LR +T+L+S++ +EIG +QP+A +G I + + L HTDA Q G Sbjct: 126 SGDGLIDLELLEKSLRPDTILLSIMAANNEIGVIQPLAAIGAICRQYQVLLHTDAAQAIG 185 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237 ++PLDV+ + +DL+SL++HK++G G GALY+R P V L + GGGQE GLRSGT Sbjct: 186 KIPLDVEEMNIDLMSLTAHKVHGPKGIGALYVRRRNPRVRLAAQIQGGGQEKGLRSGTIF 245 Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297 TP I GF A EL + +E +T L L+ RL+ I++ + + G RL +L+ + Sbjct: 246 TPQIVGFAKAVELGIKAIEEDTSYLNQLKARLWEIISQLDRIYLNGHPSQRLAGNLNISI 305 Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356 E DG + L L I +S+G+AC+S +PS +L A+G + A +R L Sbjct: 306 EGVDGAAL-------LLGLQPIVALSSGSACSSSHTAPSHVLTALGRPESLAYASLRFGL 358 Query: 357 GKQTTAADIDWTG 369 + T +I+ G Sbjct: 359 SRFNTLEEIELVG 371 >MICAK_670002 sufS cysteine desulfurase Length = 420 Score = 83.6 bits (205), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 69/233 (29%), Positives = 114/233 (48%), Gaps = 11/233 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58 +YLD SA T +P I +++T+ + +++H + RA E AR +VA IN Sbjct: 30 IYLD-SAATSQKP--IQVLETLRNYYEKDNANVHRGAHTLSVRATEAYEGARDKVARFIN 86 Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115 A + I++T TEA NL A +I+S +EH + P +++ + G I Sbjct: 87 AASRQEIVYTRNATEAINLVAYSWALNTLKAGDEIILSVMEHHSNLIPWQIVCQKTGAVI 146 Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 + + + + E K+ L T LV+V++ + +G + P+A++ + GA DA Sbjct: 147 KYVQLTSEESFDLEQYKSLLSDKTKLVAVLHVSNTLGCINPVAKIVSLAHQVGAKVLIDA 206 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227 Q L +DVQ++ D L S HK+ G G LY + + E MP GGG+ Sbjct: 207 CQSVPHLAIDVQSIDCDWLVASGHKMCAPTGIGFLYGKETILEAMPPFFGGGE 259 >MICAK_2800004 L-cysteine/cystine lyase Length = 404 Score = 57.4 bits (137), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 56/216 (25%), Positives = 95/216 (43%), Gaps = 16/216 (7%) Query: 16 EAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADN 75 EAI L Q+ +++W + +L R ++A + P ++ T T N Sbjct: 48 EAIIDAHNFLQQKGPFSGRVNDWITKKTELL---RQEMAQELGISPSTLSITEDVTVGCN 104 Query: 76 LAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITRLGVNHQ-GRVNP-EDLK 132 +A+ GV + +H++++ EH I + + + EI+ + NP E + Sbjct: 105 IALWGVD--WQAGEHILLTDCEHPGIIATVREIARRYHLEISTCPIRETLNGGNPIEVIS 162 Query: 133 AALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA-----LFHTDAVQVAGRLPLDVQ 187 A LR T ++ V + G V P+ E+ ++ + L DA Q G LPLD+ Sbjct: 163 AHLRPKTRVLVVSHVLWNTGQVLPLKEISQLCHDNSVTEKPVLVVVDAAQSVGCLPLDLS 222 Query: 188 TLPVDLLSLSSHKLYGG-LGAGALYLRPGVELMPLL 222 D + + HK + G G G LY+RP E+ P L Sbjct: 223 ATAADCYAFTGHKWWCGPAGVGGLYIRP--EIFPSL 256 >MICAK_170013 Cysteine desulfurase like Length = 386 Score = 43.1 bits (100), Expect = 2e-05, Method: Compositional matrix adjust. Identities = 40/174 (22%), Positives = 80/174 (45%), Gaps = 7/174 (4%) Query: 45 ILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104 +L+ AR ++A L++ + ++F T A N + + + + ++I+ ++A + Sbjct: 53 LLDIARQKLADLVSVNSDDLVFVPNATTAVNAVLNSLT--FQENEEILITDQTYNACANA 110 Query: 105 AKML-ENWGWE--ITRLGVNHQGRVN-PEDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160 K + + WG + I ++ Q + + + A++ T LV + + S + PIAE+ Sbjct: 111 VKHIAKRWGLKVIIAKIPFPVQSPLEISQAILASVSPRTKLVVLDHVTSPTALIWPIAEI 170 Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213 + G D G LPL++ T+ + + HK L GA LY+R Sbjct: 171 VQELNNQGIDTLIDGAHALGFLPLNIGTINPTYYTANCHKWLCSAKGAAFLYVR 224 Database: GCA_000312285.1P Posted date: Aug 27, 2016 10:38 PM Number of letters in database: 1,237,661 Number of sequences in database: 4483 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4483 Number of Hits to DB: 931,769 Number of extensions: 39009 Number of successful extensions: 107 Number of sequences better than 1.0: 6 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 1,237,661 Length adjustment: 84 Effective length of query: 298 Effective length of database: 861,089 Effective search space: 256604522 Effective search space used: 256604522 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)