BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000312265.1P
4680 sequences; 1,274,649 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MICAH_4770011 iscS Cysteine desulfurase 535 0.0
MICAH_2390014 NIFS Cysteine desulfurase 1, mitochondrial 244 2e-77
MICAH_160007 hypothetical protein 89 1e-22
MICAH_2800006 sufS cysteine desulfurase 85 7e-19
MICAH_1580004 Cysteine desulfurase 73 6e-15
MICAH_350001 L-cysteine/cystine lyase 55 2e-09
MICAH_1860002 Cysteine desulfurase like 40 2e-04
>MICAH_4770011 iscS Cysteine desulfurase
Length = 388
Score = 535 bits (1378), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 258/380 (67%), Positives = 308/380 (81%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR QVA LINA
Sbjct: 1 MQIYLDYSATTPTHPQVIERVATILQHHWGNPSSLHTWGQDTATVIEMAREQVAELINAN 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P+ IIFTSGGTEADNLAI+GVA+ Y P+H+IISSVEHSAI+EP K LE GW+ITRL V
Sbjct: 61 PDQIIFTSGGTEADNLAIIGVAQQYNRPRHIIISSVEHSAIAEPCKQLEQQGWQITRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N QGRVNP DLKAA++ +TVL+S+IYGQSE+GT+QPI ELG I + G LFHTDAVQVA
Sbjct: 121 NRQGRVNPLDLKAAIQSDTVLISIIYGQSEVGTLQPIEELGSIARERGVLFHTDAVQVAA 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R +DV+ LPVDLLSLSSHK+YG G+GALY+R GV+++PLL GGGQE GLRSGT A PA
Sbjct: 181 RCDIDVRKLPVDLLSLSSHKIYGMQGSGALYVRAGVDILPLLRGGGQEKGLRSGTPAVPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IA FG+AAELA ++L +E RLI LRDRLF +LAD P L+PTGD+ +RLPHH+SF +
Sbjct: 241 IAAFGLAAELAQKDLISEKMRLIALRDRLFDLLADYPYLLPTGDRFYRLPHHVSFIVRPD 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
D I+GK LVRQLNLAGIGIS+G+AC+SGKLSPSPIL AMGYS + AL GIRLTLG+ T
Sbjct: 301 DDSHITGKQLVRQLNLAGIGISSGSACHSGKLSPSPILKAMGYSDREALAGIRLTLGRDT 360
Query: 361 TAADIDWTGIVVKQILQRLI 380
+AADIDWT +V+KQ++ R +
Sbjct: 361 SAADIDWTALVLKQVIDRCL 380
>MICAH_2390014 NIFS Cysteine desulfurase 1, mitochondrial
Length = 387
Score = 244 bits (624), Expect = 2e-77, Method: Compositional matrix adjust.
Identities = 148/373 (39%), Positives = 220/373 (58%), Gaps = 13/373 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P+ +A + T+ +GN SS+ H +G A ++ AR +A IN+ P
Sbjct: 6 IYLDCHATTPMEPQVLAAMLPYFTEHFGNASSINHVYGWTAEAAVKQARETIAAAINSSP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLAI GVA Y +H++ EH A+ +P LE G+E+T L V
Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFAKGRHIVTVMTEHRAVLDPCHYLEKLGFEVTILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ E L+ +LR +T+L+S++ +EIG +QP+A +G I + + LFHTDA Q G
Sbjct: 126 GADGLIDLELLEKSLRPDTILLSIMAANNEIGVIQPLAAIGAICRQYQVLFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK++G G GALY+R P V L + GGGQE GLRSGT
Sbjct: 186 KIPLDVEEMNIDLMSLTAHKVHGPKGIGALYVRRRNPRVRLAAQIQGGGQEKGLRSGTIF 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A EL + +E + L L+ RL+ I++ + + G RL +L+ +
Sbjct: 246 TPQIVGFAKAVELGIKAIEEDNSHLNQLKARLWEIISQLDRIYLNGHPSQRLAGNLNISI 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L I +S+G+AC+S +PS +L A+G + A +R L
Sbjct: 306 EGVDGAAL-------LLGLQPIVALSSGSACSSSHTAPSHVLTALGRPESLAYASLRFGL 358
Query: 357 GKQTTAADIDWTG 369
+ T +I+ G
Sbjct: 359 SRFNTLEEIELVG 371
>MICAH_160007 hypothetical protein
Length = 102
Score = 89.4 bits (220), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 43/75 (57%), Positives = 51/75 (68%), Gaps = 2/75 (2%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR QVAGLINA
Sbjct: 1 MQIYLDYSATTPTHPQVIERVATILRHHWGNPSSLHSWGQDTATVIEMAREQVAGLINAN 60
Query: 61 PES--IIFTSGGTEA 73
P+ + F G +A
Sbjct: 61 PDPDYLHFWRHGYQA 75
>MICAH_2800006 sufS cysteine desulfurase
Length = 420
Score = 84.7 bits (208), Expect = 7e-19, Method: Compositional matrix adjust.
Identities = 70/233 (30%), Positives = 112/233 (48%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
+YLD SA T +P I +++T+ + +++H + RA E AR +VA IN
Sbjct: 30 IYLD-SAATSQKP--IQVLETLRNYYEKDNANVHRGAHTLSVRATEAYEGARDKVARFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T TEA NL A +I+S +EH + P +++ + G I
Sbjct: 87 AASRQEIVYTRNATEAINLVAYSWALNTLKAGDEIILSVMEHHSNLIPWQIVCQKTGAVI 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + E K+ L T LV+V++ + +G + P+AE+ + GA DA
Sbjct: 147 KYVQLTSDESFDLEQYKSLLSDKTKLVAVLHVSNTLGCINPVAEIVSLAHQVGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227
Q L +DVQ + D L S HK+ G G LY + + E MP GGG+
Sbjct: 207 CQSVPHLAIDVQAIDCDWLVASGHKMCAPTGIGFLYGKEAILEAMPPFFGGGE 259
>MICAH_1580004 Cysteine desulfurase
Length = 572
Score = 73.2 bits (178), Expect = 6e-15, Method: Compositional matrix adjust.
Identities = 102/390 (26%), Positives = 157/390 (40%), Gaps = 48/390 (12%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
++ D +ATT +P+A+ ++ + + HE R+ E AR V +NA
Sbjct: 193 IWFDNAATT-QKPQAVIDRLVHFYSHENSNVHRAAHELAARSTDAYEAARETVRRFLNAS 251
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCY-----TTPQHMIISSVEHSAISEPAKMLE-NWGW 113
IIF G TE NL VA+ + ++++ +EH A P + L G
Sbjct: 252 SVNEIIFVRGTTEGINL----VAKSWGQQTLQAGDEIVLTHLEHHANIVPWQQLAAQTGA 307
Query: 114 EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173
++ + V+ G++ + + L T LV+ + + +GT+ P ++ + GA
Sbjct: 308 KLRVVPVDDHGQILLAEYQQLLNSRTKLVAFSHVSNALGTITPAKQIIDLAHRVGAKVLL 367
Query: 174 DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGGQENGL 231
D Q +P+DVQ L D S HK++G G G LY + + +P GG +
Sbjct: 368 DGAQSVSHIPIDVQQLGCDWFVFSGHKVFGPTGIGVLYGQEDLLNATVPWQSGGNMIVDV 427
Query: 232 ---RSGTQATPA-----------IAGFGVAAELAAQ-ELETETGRLILLRDRLFAILADV 276
R+ QA P G G A + Q L T L + A L +
Sbjct: 428 TFERTVYQAAPTRFEAGTGNIADAVGLGTALDYVQQIGLTTIANYEHELLEYATAGLTTI 487
Query: 277 PGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSP 336
PGL G + LSF LE D E I + LN GI + AG C P
Sbjct: 488 PGLRLIGTAADKAA-VLSFVLEGFDVEVIG-----QALNREGIAVRAGHHCA------QP 535
Query: 337 ILLAMGYSQKAALGGIRLTLGKQTTAADID 366
IL G +R +L T A++D
Sbjct: 536 ILRRFGLE-----ATVRPSLAFYNTKAEVD 560
>MICAH_350001 L-cysteine/cystine lyase
Length = 363
Score = 55.5 bits (132), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 50/185 (27%), Positives = 84/185 (45%), Gaps = 13/185 (7%)
Query: 47 ETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAI-SEPA 105
E R ++A + P ++ T T N+A+ GV + +H++++ EH I +
Sbjct: 35 ELLRQEMAQELGISPSTLSITEDVTVGCNIALWGVD--WQAGEHILLTDCEHPGIIATVQ 92
Query: 106 KMLENWGWEITRLGVNHQ-GRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKI 163
++ + EI+ + NP E + A LR T ++ V + G V P+ E+ ++
Sbjct: 93 EIARRYHLEISTCPIRETLNGGNPIEVISAHLRPKTRVLVVSHVLWNTGQVLPLKEISQL 152
Query: 164 TKTHGA-----LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGVE 217
+ L DA Q G LPLD+ D + + HK + G G G LY+RP E
Sbjct: 153 CHDNSVTEKPVLVVVDAAQSVGCLPLDLSATAADCYAFTGHKWWCGPAGVGGLYIRP--E 210
Query: 218 LMPLL 222
+ P L
Sbjct: 211 IFPSL 215
>MICAH_1860002 Cysteine desulfurase like
Length = 401
Score = 40.0 bits (92), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 39/174 (22%), Positives = 78/174 (44%), Gaps = 7/174 (4%)
Query: 45 ILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
+L+ AR ++A L+ + ++F T A N + + + + ++I+ ++A +
Sbjct: 53 LLDIARQKLADLVGVNSDDLVFVPNATTAVNAVLNSLT--FQENEEILITDQTYNACANA 110
Query: 105 AKML-ENWGWE--ITRLGVNHQGRVN-PEDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
K + + WG + I ++ Q + + + A++ T LV + + S + PIAE+
Sbjct: 111 VKHIAKRWGLKVIIAKIPFPVQSPLEISQAILASVSPRTKLVVLDHVTSPTALIWPIAEI 170
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213
+ G D G LPL++ + + + HK L GA LY+R
Sbjct: 171 VRELNNRGIDTLIDGAHALGFLPLNIGAINPTYYTANCHKWLCSPKGAAFLYVR 224
Database: GCA_000312265.1P
Posted date: Aug 27, 2016 10:56 PM
Number of letters in database: 1,274,649
Number of sequences in database: 4680
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4680
Number of Hits to DB: 958,759
Number of extensions: 40030
Number of successful extensions: 129
Number of sequences better than 1.0: 7
Number of HSP's gapped: 124
Number of HSP's successfully gapped: 7
Length of query: 382
Length of database: 1,274,649
Length adjustment: 85
Effective length of query: 297
Effective length of database: 876,849
Effective search space: 260424153
Effective search space used: 260424153
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)