BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000312245.1P
4845 sequences; 1,312,278 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MICAG_480002 iscS Cysteine desulfurase 538 0.0
MICAG_3230007 iscS Cysteine desulfurase 246 6e-78
MICAG_3630009 sufS cysteine desulfurase 85 5e-19
MICAG_310006 csd putative cysteine desulfurase 1 72 1e-14
MICAG_3380004 Genome sequencing data, contig C316 58 4e-10
MICAG_1880023 Genome sequencing data, contig C320 39 3e-04
>MICAG_480002 iscS Cysteine desulfurase
Length = 388
Score = 538 bits (1387), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 260/380 (68%), Positives = 311/380 (81%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT P+ I + TIL WGNPSSLH WG A ++E AR QVAGLINA
Sbjct: 1 MQIYLDYSATTPTHPQVIERVATILRHHWGNPSSLHTWGQDTATVIEMAREQVAGLINAN 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P+ IIFTSGGTEADNLAI+GVA+ Y P+H+IISSVEHSAI+EP K LE GW+ITRL V
Sbjct: 61 PDQIIFTSGGTEADNLAIIGVAQQYNRPRHIIISSVEHSAIAEPCKQLEQQGWQITRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N QGRVNP DLKAA++ +TVL+S+IYGQSE+GT+QPI ELG+I + G LFHTDAVQVA
Sbjct: 121 NRQGRVNPLDLKAAIQSDTVLISIIYGQSEVGTLQPIEELGRIARERGVLFHTDAVQVAA 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R +DV+ LPVDLLSLSSHK+YG G+GALY+R GV+++PLL GGGQE GLRSGTQA PA
Sbjct: 181 RCDIDVRKLPVDLLSLSSHKIYGMQGSGALYIRAGVDILPLLRGGGQEKGLRSGTQAVPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IA FG+AAELA ++L +E RL+ LRDRLF +LAD P L+PTGD+ +RLPHH+SF +
Sbjct: 241 IAVFGLAAELAQKDLISEKMRLMGLRDRLFDLLADYPYLLPTGDRFYRLPHHVSFIVRPD 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
D I+GK LVRQLNLAGIGIS+GAAC+SGKLSPSPIL AMGYS + AL GIRLTLG+ T
Sbjct: 301 DDSHITGKQLVRQLNLAGIGISSGAACHSGKLSPSPILKAMGYSDREALAGIRLTLGRDT 360
Query: 361 TAADIDWTGIVVKQILQRLI 380
+AADIDWT +V+KQ++ R +
Sbjct: 361 SAADIDWTALVLKQVIDRCL 380
>MICAG_3230007 iscS Cysteine desulfurase
Length = 387
Score = 246 bits (627), Expect = 6e-78, Method: Compositional matrix adjust.
Identities = 149/373 (39%), Positives = 221/373 (59%), Gaps = 13/373 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P+ +A + T+ +GN SS+ H +G A ++ AR +A IN+ P
Sbjct: 6 IYLDCHATTPMEPQVLAAMLPYFTEHFGNASSINHVYGWTAEAAVKQARETIAAAINSSP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TEA+NLAI GVA Y +H++ EH A+ +P LE G+E+T L V
Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFAKGRHIVTVMTEHRAVLDPCHYLEKLGFEVTILPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ G ++ E L+ +LR +T+L+S++ +EIG +QP+A +G I + + LFHTDA Q G
Sbjct: 126 SGDGLIDLELLEKSLRPDTILLSIMAANNEIGVIQPLAAIGAICRQYQVLFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK++G G GALY+R P V L + GGGQE GLRSGT
Sbjct: 186 KIPLDVEEMNIDLMSLTAHKVHGPKGIGALYVRRRNPRVRLAAQIQGGGQEKGLRSGTLF 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A EL + +E +T L L+ RL+ I+ + + G RL +L+ +
Sbjct: 246 TPQIVGFAKAVELGIKAIEEDTSHLNQLKARLWEIINQLDRIYLNGHPSQRLAGNLNISI 305
Query: 298 EAADGEKISGKTLVRQLNLAGI-GISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
E DG + L L I +S+G+AC+S +PS +L A+G + A +R L
Sbjct: 306 EGVDGAAL-------LLGLQPIVALSSGSACSSSHTAPSHVLTALGRPESLAYASLRFGL 358
Query: 357 GKQTTAADIDWTG 369
+ T +I+ G
Sbjct: 359 SRFNTLEEIELVG 371
>MICAG_3630009 sufS cysteine desulfurase
Length = 420
Score = 85.1 bits (209), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 102/353 (28%), Positives = 155/353 (43%), Gaps = 36/353 (10%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
+YLD SA T +P I +++T+ + +++H + RA E AR +VA IN
Sbjct: 30 IYLD-SAATSQKP--IQVLETLRNYYEKDNANVHRGAHTLSVRATEAYEGARDKVARFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T TEA NL A +I+S +EH + P +++ + G I
Sbjct: 87 AASRQEIVYTRNATEAINLVAYSWALNTLKAGDEIILSVMEHHSNLIPWQIVCQKTGAVI 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + E K+ L T LV+V++ + +G + P+AE+ + GA DA
Sbjct: 147 KYVQLTSDESFDLEQYKSLLSDKTKLVAVLHVSNTLGCINPVAEIVSLAHQVGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ---ENGL 231
Q L +DVQ++ D L S HK+ G G LY + + E MP GGG+ E L
Sbjct: 207 CQSVPHLAIDVQSIDCDWLVASGHKMCAPTGIGFLYGKEAILEAMPPFFGGGEMIAEVYL 266
Query: 232 RSGTQA---------TPAIA---GFGVAAE-LAAQELETETGRLILLRDRLFAILADVPG 278
T A TPAI G A E L ++ L LF L ++ G
Sbjct: 267 DHFTCAELPHKFEAGTPAIGEAIALGAAVEYLMGLGMDNIHAYEEELTAHLFKKLGEIEG 326
Query: 279 LIPTGDKIHRLPHHLS----FYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
L +I+ P L+ L A + E I L L+ GI I +G C
Sbjct: 327 L-----RIYGPPPSLTGQGRASLAAFNVEGIHASDLATLLDHEGIAIRSGHHC 374
>MICAG_310006 csd putative cysteine desulfurase 1
Length = 560
Score = 72.4 bits (176), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 101/390 (25%), Positives = 157/390 (40%), Gaps = 48/390 (12%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
++ D +ATT +P+A+ ++ + + HE R+ E AR V +NA
Sbjct: 181 IWFDNAATT-QKPQAVIDRLVHFYSHENSNVHRAAHELAARSTDAYEAARETVRRFLNAS 239
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCY-----TTPQHMIISSVEHSAISEPAKMLE-NWGW 113
IIF G TE NL VA+ + ++++ +EH A P + L G
Sbjct: 240 SANEIIFVRGTTEGINL----VAKSWGQQTLQAGDEIVLTHLEHHANIVPWQQLAAQTGA 295
Query: 114 EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173
++ + V+ G++ + + L T LV+ + + +GT+ P ++ + GA
Sbjct: 296 KLRVVPVDDHGQILLAEYQQLLNSRTKLVAFSHVSNALGTITPAKQIIDLAHRVGAKVLL 355
Query: 174 DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGGQENGL 231
D Q +P+DVQ L D S HK++G G G LY + + +P GG +
Sbjct: 356 DGAQSVSHIPIDVQQLGCDWFVFSGHKVFGPTGIGVLYGQEDLLNATVPWQSGGNMIVDV 415
Query: 232 ---RSGTQATPA-----------IAGFGVAAELAAQ-ELETETGRLILLRDRLFAILADV 276
R+ QA P G G A + Q L T L + A L +
Sbjct: 416 TFERTVYQAAPTRFEAGTGNIADAVGLGTALDYVQQIGLTTIANYEHELLEYATAGLTTI 475
Query: 277 PGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSP 336
PGL G + LSF LE D E + + LN GI + AG C P
Sbjct: 476 PGLRLIGTAADKA-GVLSFVLEGFDVEVVG-----QALNREGIAVRAGHHCA------QP 523
Query: 337 ILLAMGYSQKAALGGIRLTLGKQTTAADID 366
IL G +R +L T A++D
Sbjct: 524 ILRRFGLE-----ATVRPSLAFYNTKAEVD 548
>MICAG_3380004 Genome sequencing data, contig C316
Length = 404
Score = 57.8 bits (138), Expect = 4e-10, Method: Compositional matrix adjust.
Identities = 63/234 (26%), Positives = 105/234 (44%), Gaps = 21/234 (8%)
Query: 2 QVYLDYSATTPTRPEA--IAIIQTILTQQWGNPSS--LHEWGNRAALILETARMQVAGLI 57
+VY ++ T P+A AII T Q P S +++W + +L R ++A +
Sbjct: 31 KVYFNFGGQG-TLPKAGLEAIIDTYNFLQQKGPFSGQVNDWITKKTELL---RQEMAQEL 86
Query: 58 NAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEIT 116
P ++ T T N+A+ GV + +H++++ EH I + + + EI+
Sbjct: 87 GISPRTLSITEDVTVGCNIALWGVD--WQAGEHILLTDCEHPGIIATVREIARRYHLEIS 144
Query: 117 RLGVNHQ-GRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA----- 169
+ NP E + A LR T ++ V + G V P+ E+ ++ +
Sbjct: 145 TCPIRETLNGGNPIEVISAHLRPKTRVLVVSHVLWNTGQVLPLKEISQLCHDNSVTEKPV 204
Query: 170 LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGVELMPLL 222
L DA Q G LPLD+ D + + HK + G G G LY+RP E+ P L
Sbjct: 205 LVVVDAAQSVGCLPLDLSATAADCYAFTGHKWWCGPAGVGGLYIRP--EIFPSL 256
>MICAG_1880023 Genome sequencing data, contig C320
Length = 386
Score = 39.3 bits (90), Expect = 3e-04, Method: Compositional matrix adjust.
Identities = 39/174 (22%), Positives = 78/174 (44%), Gaps = 7/174 (4%)
Query: 45 ILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
+L+ AR ++A L+ + ++F T A N + + + + ++I+ ++A +
Sbjct: 53 LLDIARQKLADLVGVNSDDLVFVPNATTAVNAVLNSLT--FQENEEILITDQTYNACANA 110
Query: 105 AKML-ENWGWE--ITRLGVNHQGRVN-PEDLKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
K + + WG + I ++ Q + + + A++ T LV + + S + PIAE+
Sbjct: 111 VKHIAKRWGLKVIIAKIPFPVQSPLEISQAILASVSPRTKLVVLDHVTSPTALIWPIAEI 170
Query: 161 GKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLR 213
+ G D G LPL++ + + + HK L GA LY+R
Sbjct: 171 VQELNNRGIDTLIDGAHALGFLPLNIGAINPTYYTANCHKWLCSPKGAAFLYVR 224
Database: GCA_000312245.1P
Posted date: Aug 27, 2016 10:46 PM
Number of letters in database: 1,312,278
Number of sequences in database: 4845
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4845
Number of Hits to DB: 986,094
Number of extensions: 41124
Number of successful extensions: 123
Number of sequences better than 1.0: 6
Number of HSP's gapped: 118
Number of HSP's successfully gapped: 6
Length of query: 382
Length of database: 1,312,278
Length adjustment: 85
Effective length of query: 297
Effective length of database: 900,453
Effective search space: 267434541
Effective search space used: 267434541
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)