BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000309945.1P
4780 sequences; 1,548,170 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
OsccyDRAFT_2706 cysteine desulfurase family protein 578 0.0
OsccyDRAFT_3946 cysteine desulfurase family protein 294 2e-96
OsccyDRAFT_4965 cysteine desulfurase NifS 278 4e-90
OsccyDRAFT_3705 cysteine desulfurase family protein 206 1e-62
OsccyDRAFT_3042 cysteine desulfurase-like protein, SufS subfamily 80 4e-17
OsccyDRAFT_1912 selenocysteine lyase 64 6e-12
OsccyDRAFT_2715 serine-pyruvate aminotransferase/archaeal aspart... 35 0.007
OsccyDRAFT_2390 selenocysteine lyase 32 0.061
OsccyDRAFT_2757 nucleoside-diphosphate-sugar epimerase 31 0.15
OsccyDRAFT_1125 glyceraldehyde 3-phosphate dehydrogenase (NADP+) 30 0.25
>OsccyDRAFT_2706 cysteine desulfurase family protein
Length = 388
Score = 578 bits (1489), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 280/381 (73%), Positives = 322/381 (84%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTP RPEAIA +Q I + QWGNPSSLH+WG RAA LE AR+QVA LINA
Sbjct: 1 MQIYLDYSATTPLRPEAIAAMQQIFSDQWGNPSSLHQWGQRAATALERARVQVARLINAP 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E+I+FTSGGTEADNLA+ GVAR Y+ PQH+IISS+EHSA+SEPA+ LE GW++TRL V
Sbjct: 61 EEAIVFTSGGTEADNLALFGVARQYSEPQHLIISSIEHSAVSEPARFLEQQGWQVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ QGR+NP DL+AAL+ NTVLVS+IYGQSE+GT+Q I L I +HGALFHTDAVQVAG
Sbjct: 121 DQQGRINPVDLRAALQSNTVLVSIIYGQSEVGTLQAIPTLSAIAHSHGALFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLP+DVQ PVDLLSLSSHKLYG GAGALY+RPGVELMP LGGGGQE+GLRSGTQA PA
Sbjct: 181 RLPIDVQQFPVDLLSLSSHKLYGPQGAGALYVRPGVELMPFLGGGGQESGLRSGTQALPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFGVAAELAAQE+ ET RL+ LRDRLFA L+DVPGL PTGD+ RLPHH SF +E A
Sbjct: 241 IAGFGVAAELAAQEILVETQRLLELRDRLFAQLSDVPGLRPTGDRWQRLPHHASFCVEYA 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
DGE ++GK LVRQ+NLAGIGISAGAAC SGK++PSP+L AMGYS + A GIRLTLG+ T
Sbjct: 301 DGEVLTGKALVRQMNLAGIGISAGAACRSGKVTPSPVLKAMGYSDRVAKSGIRLTLGRHT 360
Query: 361 TAADIDWTGIVVKQILQRLIP 381
T DIDWT IV+KQ+L+RL P
Sbjct: 361 TLEDIDWTTIVLKQVLERLQP 381
>OsccyDRAFT_3946 cysteine desulfurase family protein
Length = 391
Score = 294 bits (753), Expect = 2e-96, Method: Compositional matrix adjust.
Identities = 159/369 (43%), Positives = 234/369 (63%), Gaps = 11/369 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP P + ++ T+ +GNP+S+ H++G A ++ +R +A INA+P
Sbjct: 8 IYLDCHATTPVDPRVVEVMLPFFTEFFGNPASISHQYGWEADAAVQQSRELLALAINAMP 67
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y + +H+I EH+A+ +P + L + G++IT L V
Sbjct: 68 EEIVFTSGATEANNLAIKGVAEAYCSRGKHIITVETEHNAVLDPCRYLRSLGFDITFLSV 127
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ DL+ ALR +T+LVSV+ +EIG +QPIAE+G + G LFHTDA Q G
Sbjct: 128 KPDGLIDLLDLENALRPDTILVSVMAANNEIGVLQPIAEIGALCHQRGILFHTDAAQAIG 187
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK+YG G GALY+R P V+L P L GGG E G+RSGT
Sbjct: 188 KVPLDVEAMHIDLMSLTAHKIYGPKGIGALYVRRRNPRVQLAPQLHGGGHERGMRSGTLY 247
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I G A +LA +E+ETET R+ LRDRL+ + G+ G RL +L+ +
Sbjct: 248 TPQIVGMAAAVQLAIEEMETETVRITQLRDRLWQAFTSLGGIYLNGHPTQRLCSNLNISI 307
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
E DG+ + LV ++ + +S+G+AC S K +PS +L A+G S + A IR +G
Sbjct: 308 EGIDGQAL----LVGLQSV--VAVSSGSACTSAKSTPSHVLQAIGRSAELAYASIRFGIG 361
Query: 358 KQTTAADID 366
+ T A++D
Sbjct: 362 RFNTEAEVD 370
>OsccyDRAFT_4965 cysteine desulfurase NifS
Length = 401
Score = 278 bits (711), Expect = 4e-90, Method: Compositional matrix adjust.
Identities = 158/378 (41%), Positives = 223/378 (58%), Gaps = 7/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P+ +A + LT+ +GNPSS+H +G + L+ AR QVA L+ A
Sbjct: 5 IYLDNNATTKVDPDVLAAMLPYLTEYYGNPSSMHSFGGQVGKALKQARAQVASLLGAEDS 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE +N AI +H+I + VEH+ + K LE G+ +T L VN
Sbjct: 65 EIVFTSCGTEGNNTAIRAALAAQPDRKHLITTQVEHACVLNLFKQLEKQGYSVTYLSVNR 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G+V+ +L+AA+ NT LVS++Y +E GTV P+ + G I K +GA FH DAVQVAG+L
Sbjct: 125 KGQVDLMELEAAMTGNTALVSMMYANNETGTVFPVEQAGAIAKEYGAAFHVDAVQVAGKL 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++ +DL+++S HKL+ G GALY+R G PLL GG QE G R+GT+ I
Sbjct: 185 PLNMKISTIDLMTISGHKLHAPKGIGALYVRRGFRFRPLLIGGHQERGRRAGTENVAGII 244
Query: 243 GFGVAAELAAQELETETGRLILLRDRL-FAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G G AAELA L + LRDRL IL +P GD +RLP+ + + +
Sbjct: 245 GLGKAAELAQVHL-ADVAAEKKLRDRLEKGILTRIPNCEVNGDPKNRLPNTTNIGFKYIE 303
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + L+ G+ S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 304 GEAI-----LLSLDKFGVCASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLSRYTT 358
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID ++ QI++RL
Sbjct: 359 EAEIDRVLELLPQIIERL 376
>OsccyDRAFT_3705 cysteine desulfurase family protein
Length = 391
Score = 206 bits (524), Expect = 1e-62, Method: Compositional matrix adjust.
Identities = 130/376 (34%), Positives = 199/376 (52%), Gaps = 19/376 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY ATTP I +T+++GN SS H WG+RA ++ A QVA LI A P
Sbjct: 2 IYLDYHATTPVDRRVADRIHHYMTEEFGNASSTDHAWGDRAEAAVKQAAQQVADLIGASP 61
Query: 62 ESIIFTSGGTEADNLAIMG-----VARCYTTP----QHMI-ISSVEHSAISEPAKMLENW 111
+ II+TSG TE+ NLAI G ++R +H I + +EH A+ + + LE
Sbjct: 62 KEIIWTSGATESINLAIQGSLLAPLSRSGRGAGGEGKHRIALMPLEHKAVLDTCRALEKR 121
Query: 112 GW-EITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGAL 170
GW E+ L V+ +GR++ L+ A L+ V+ +EIG + PI ++G++ + +G
Sbjct: 122 GWAELVYLQVDSKGRLDLNHLEQACAEGLSLLCVMAANNEIGNIYPIQKIGQMAQHYGIP 181
Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENG 230
F DA Q G++P+ + + L++S+HK YG G GAL +R G L PL+ GGG + G
Sbjct: 182 FLCDASQAVGKIPVQFEAWGITYLAISAHKFYGPKGVGALVVRKGYHLEPLIFGGGHQRG 241
Query: 231 LRSGTQATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRL 289
+RSGT P I G G A L + E+E + + + RDRL +L + +P L+ GD RL
Sbjct: 242 MRSGTLNVPGIVGLGEACRLRSLEMEEDERAIAIKRDRLQDLLLEKIPDLVINGDLEQRL 301
Query: 290 PHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAAL 349
L + + + + + IS G+AC+S PS +L M K
Sbjct: 302 AGSLHISIPGIPNSAVIARVRSK------LAISTGSACSSNVEEPSHVLRTMKLQDKVID 355
Query: 350 GGIRLTLGKQTTAADI 365
G +R+ +GK + +I
Sbjct: 356 GALRIGIGKFSQDEEI 371
>OsccyDRAFT_3042 cysteine desulfurase-like protein, SufS subfamily
Length = 420
Score = 79.7 bits (195), Expect = 4e-17, Method: Compositional matrix adjust.
Identities = 69/234 (29%), Positives = 115/234 (49%), Gaps = 16/234 (6%)
Query: 3 VYLDYSATTPTRPEAIAIIQTIL-TQQWGNPS---SLHEWGNRAALILETARMQVAGLIN 58
+YLD +AT+ +P I++++ + ++ N + +H NRA E AR +VA IN
Sbjct: 30 IYLDNAATS-QKP--ISVLEALTHYYEYDNANVHRGVHTLSNRATDAYEGARDKVADFIN 86
Query: 59 AVP-ESIIFTSGGTEADNL-AIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T +EA NL A T +I++ +EH + P + + + G +
Sbjct: 87 AASRQEIVYTRNASEAINLVAYAWGMNTLQTGDEIILTVMEHHSNLIPWQFVAQRTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + K+ L T LVSV++ + +G + P+ E+ +GA DA
Sbjct: 147 KFVELTETEEFDFDHFKSLLSDKTKLVSVVHVSNTLGCINPVKEICAEAHRYGAKVLVDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELM----PLLGGG 225
Q A + LDVQ + D L S HK+ G G LY + +EL+ P LGGG
Sbjct: 207 CQSAPHMLLDVQEIDCDWLVASGHKMCAPTGIGFLYGK--LELLRSMPPFLGGG 258
>OsccyDRAFT_1912 selenocysteine lyase
Length = 398
Score = 63.5 bits (153), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 85/309 (27%), Positives = 130/309 (42%), Gaps = 41/309 (13%)
Query: 2 QVYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSLHEWGNRAALILETA---RMQVAGLI 57
+ Y +Y P A+ AI Q+ T Q P S G L+ + A R +A +
Sbjct: 24 KAYFNYGGQGPMPQIALDAIAQSFQTIQQYGPFS----GKAGVLVTQEAELTRGAIAQEL 79
Query: 58 NAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML-ENWGWEIT 116
N PE+I T T N+A+ G+ + H+++S EH I A+ + +G E+
Sbjct: 80 NVSPETITLTEDVTVGCNIAMWGID--WRLGDHLLLSDCEHPGIVAAAQEIARRFGVEVN 137
Query: 117 RLGVNHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAEL----GKITKTHG 168
+ Q +N D + + L+ +T LV + + G V P+AE+ + +H
Sbjct: 138 ICPL--QATLNQGDPVAVVASQLKPSTRLVVLSHILWNTGQVLPLAEIVAACHSYSHSHP 195
Query: 169 ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGV--ELMP-LLGG 224
DA Q G LPL++ +L D + + HK + G G G LY+RP L P +G
Sbjct: 196 VKVLVDAAQSVGVLPLNLASLQADFYAFTGHKWWCGPEGVGGLYVRPEAMSSLHPTFIGW 255
Query: 225 GG---QENGLRSG-----------TQATPAIAGFGVAAELAAQ--ELETETGRLILLRDR 268
G E G +G T A P AG A L Q + R+ L
Sbjct: 256 RGITKDETGSPTGWLPDARRYEVATSAYPLYAGLRQAIALHHQFGTPQERFQRIRTLSKM 315
Query: 269 LFAILADVP 277
L+ L D+P
Sbjct: 316 LWQALVDIP 324
>OsccyDRAFT_2715 serine-pyruvate aminotransferase/archaeal aspartate
aminotransferase
Length = 383
Score = 35.4 bits (80), Expect = 0.007, Method: Compositional matrix adjust.
Identities = 21/79 (26%), Positives = 40/79 (50%), Gaps = 4/79 (5%)
Query: 130 DLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQT 188
+L+ A+ H ++++++ ++ G QP+ +G++ + G L D V G +P+ +
Sbjct: 138 ELRTAMETHRPAILALVHAETSTGAHQPLEGVGELCREFGCLLLVDMVTSLGGVPIFLDE 197
Query: 189 LPVDLLSLSSHKLYGGLGA 207
VDL S K GLG
Sbjct: 198 WGVDLAYSCSQK---GLGC 213
>OsccyDRAFT_2390 selenocysteine lyase
Length = 400
Score = 32.3 bits (72), Expect = 0.061, Method: Compositional matrix adjust.
Identities = 24/76 (31%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
Query: 139 TVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSS 198
T LV + + S+ G + PIAEL + G D G +PL+++ L + +
Sbjct: 164 TRLVLLDHVTSQTGLIFPIAELVQELNQRGIETLIDGAHAPGMIPLNLEELGATYYTGNC 223
Query: 199 HK-LYGGLGAGALYLR 213
HK L GA LY+R
Sbjct: 224 HKWLCSPKGAAFLYVR 239
>OsccyDRAFT_2757 nucleoside-diphosphate-sugar epimerase
Length = 309
Score = 30.8 bits (68), Expect = 0.15, Method: Compositional matrix adjust.
Identities = 24/79 (30%), Positives = 30/79 (37%), Gaps = 2/79 (2%)
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
H R NP DLKA L E+ QP+AEL K H + V +
Sbjct: 46 HGDRTNPADLKAKLEGQEFDAIFDNNGRELSDTQPLAELFKDQVKHFVYMSSAGVYLKSD 105
Query: 182 LPLDVQTLPVDLLSLSSHK 200
++ PVD S HK
Sbjct: 106 QMPHIEGDPVD--PESRHK 122
>OsccyDRAFT_1125 glyceraldehyde 3-phosphate dehydrogenase (NADP+)
Length = 339
Score = 30.4 bits (67), Expect = 0.25, Method: Compositional matrix adjust.
Identities = 33/102 (32%), Positives = 49/102 (48%), Gaps = 13/102 (12%)
Query: 22 QTILTQQWGNPSSL--HEWGNRAALILE-----TARMQVAGLINAVPESIIFTSGGTEAD 74
+TI NP +L EW LI+E T+R INA + ++ T+ G D
Sbjct: 73 KTIKCTSDRNPENLPWKEW--EIDLIIESTGVFTSREGATKHINAGAKKVLITAPGKNDD 130
Query: 75 NLAIMGVA-RCYTTPQHMIIS--SVEHSAISEPAKML-ENWG 112
++GV + Y QH IIS S + ++ AK+L EN+G
Sbjct: 131 GTFVVGVNHQNYDHAQHHIISNASCTTNCLAPVAKVLHENFG 172
Database: GCA_000309945.1P
Posted date: Aug 27, 2016 10:45 PM
Number of letters in database: 1,548,170
Number of sequences in database: 4780
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4780
Number of Hits to DB: 1,204,965
Number of extensions: 52508
Number of successful extensions: 207
Number of sequences better than 1.0: 11
Number of HSP's gapped: 195
Number of HSP's successfully gapped: 11
Length of query: 382
Length of database: 1,548,170
Length adjustment: 86
Effective length of query: 296
Effective length of database: 1,137,090
Effective search space: 336578640
Effective search space used: 336578640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)