BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000307915.1P
6108 sequences; 1,647,000 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPLC1_S205650 aminotransferase class V 560 0.0
SPLC1_S204600 aminotransferase class V 290 8e-95
SPLC1_S208880 aminotransferase class V 233 6e-73
SPLC1_S030940 cysteine desulfurase SufS subfamily 81 1e-17
SPLC1_S533310 cysteine desulfurase SufS subfamily 69 3e-13
SPLC1_S101340 aminotransferase class V 43 3e-05
SPLC1_S208850 aminotransferase class V 34 0.004
SPLC1_S082020 hypothetical protein 28 0.59
>SPLC1_S205650 aminotransferase class V
Length = 389
Score = 560 bits (1442), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 278/381 (72%), Positives = 320/381 (83%), Gaps = 1/381 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPT EAIA +Q +LT+ WGNPSSLHEWG R+A ++E AR QVA LINA
Sbjct: 1 MQIYLDYSATTPTCAEAIAAMQGVLTESWGNPSSLHEWGQRSATVVEVARTQVAHLINAP 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
P+ IIFTSGGTEADNLAI GV Y PQH+IISSVEHSAI+EP +LE GW +TRL V
Sbjct: 61 PDGIIFTSGGTEADNLAIFGVVGQYDQPQHLIISSVEHSAIAEPVGLLEKRGWSVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ QGR++P DLK AL+ NTVLVSVIYGQSE+GT+QPI LGKI ++HG LFHTDAVQVAG
Sbjct: 121 DRQGRIHPLDLKTALQPNTVLVSVIYGQSEVGTLQPIETLGKIARSHGVLFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RLP+DV+ LPVD+LSLSSHK+YG G GALY+RPGVEL+PL+GGGGQE LRSGTQA PA
Sbjct: 181 RLPIDVENLPVDMLSLSSHKIYGPQGVGALYVRPGVELIPLMGGGGQEFKLRSGTQAVPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSF-YLEA 299
IAGFGVAAELAAQEL ET RLI LRDRLF +LAD+P L+PTGD++HRLPHH+SF +
Sbjct: 241 IAGFGVAAELAAQELLWETPRLIKLRDRLFDLLADIPYLVPTGDRLHRLPHHVSFCVVPP 300
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
A+ I+GKTLVR LNLAGIGISAG+AC+SGKLSPSP+LLAMGYS + LG IRLTLG+Q
Sbjct: 301 AEVTGITGKTLVRHLNLAGIGISAGSACHSGKLSPSPVLLAMGYSDREGLGSIRLTLGRQ 360
Query: 360 TTAADIDWTGIVVKQILQRLI 380
TT ADI+WT I VKQIL RLI
Sbjct: 361 TTEADIEWTAIAVKQILDRLI 381
>SPLC1_S204600 aminotransferase class V
Length = 388
Score = 290 bits (741), Expect = 8e-95, Method: Compositional matrix adjust.
Identities = 159/386 (41%), Positives = 238/386 (61%), Gaps = 14/386 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP + ++ T+ +GNP+S+ H +G ++ AR +A INA P
Sbjct: 6 IYLDCHATTPVDKRVLEVMIPYFTEHFGNPASVNHLYGWETEAAVKQARQIIADAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
IIFTSG TEA+NLAI GVA Y + +H+I + EH A+ +P L++ G+E+T LGV
Sbjct: 66 TEIIFTSGATEANNLAIKGVAEAYFSQGRHLITVATEHHAVLDPYDYLQSLGFEVTLLGV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ +L+ ALR +T+LVSV+ +EIG +QP+AE+GKI + LFHTDA Q G
Sbjct: 126 KPDGLIDLTELEEALRPDTILVSVMAANNEIGVLQPLAEIGKICRDRQILFHTDAAQALG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ + +DL+SL++HK+YG G G LY+R P V++ P + GGGQE G+RSGT
Sbjct: 186 KIPLDVEEMNIDLMSLTAHKIYGPKGIGCLYVRRRQPRVKIAPQIHGGGQERGIRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A +LA E+ TE RL LRDRL+ L+ + + G RLP +L+ +
Sbjct: 246 TPQIVGFGAAVKLALSEMATEAKRLTQLRDRLWNQLSTLDNIRLNGHPTQRLPGNLNVSI 305
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
+ DG+ + L Q +A +S+G+AC S ++ PS +L A+G + A +R +G
Sbjct: 306 DGVDGQAL---LLALQPTMA---VSSGSACTSTRIEPSHVLKALGVPENLAYASLRFGIG 359
Query: 358 KQTTAADIDWT---GIVVKQILQRLI 380
+ T A+ID I ++L+R++
Sbjct: 360 RFNTEAEIDQVAQEAIATIKVLRRVV 385
>SPLC1_S208880 aminotransferase class V
Length = 388
Score = 233 bits (594), Expect = 6e-73, Method: Compositional matrix adjust.
Identities = 140/368 (38%), Positives = 204/368 (55%), Gaps = 11/368 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLDY ATTP P I +T ++GN +S+ H +G+R + A QVA L+ A P
Sbjct: 7 IYLDYHATTPVDPRVAEKILHYMTVEFGNANSIDHIYGDRTQKAVSQAARQVAELVGASP 66
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQ--HMIISSVEHSAISEPAKMLENWGW-EITRL 118
IIFTSG TE+ NLAI G + +P + +S VEH A+ + + L G +IT L
Sbjct: 67 REIIFTSGATESINLAIQGSIINHQSPYLPRIAVSPVEHPAVLDTGQALVKQGLIDITYL 126
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
V+ QG+++ E + R L+ ++ +EIGT+ PIAE+GKI +TH F D Q
Sbjct: 127 SVDKQGQIDLESVDKVCRDRVSLLCLMAANNEIGTIYPIAEIGKIAQTHQVAFLCDGSQA 186
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQAT 238
G++P++ + + L++S HKLYG G GAL R G L P++ GGG + G+RSGT
Sbjct: 187 VGKIPINFEEWGITYLAISGHKLYGPKGVGALVARKGYPLKPMMFGGGHQGGMRSGTLNV 246
Query: 239 PAIAGFGVAAELAAQELETETGRLILLRDRLFAILAD-VPGLIPTGDKIHRLPHHLSFYL 297
P I G G A L E+ + R+ LRD+L +L + +PGL+ GD HRL +L +
Sbjct: 247 PGIVGLGEACRLRGLEMAEDEARIANLRDKLQDLLLEKIPGLVVNGDINHRLSGNLHISI 306
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
I + R + IS GAAC+SG +PS +L A+ S A G +R+ +G
Sbjct: 307 PDVPNSAIIARVRDR------LAISTGAACSSGVEAPSHVLRAIALSPNAMEGALRIGIG 360
Query: 358 KQTTAADI 365
K TT ++I
Sbjct: 361 KFTTESEI 368
>SPLC1_S030940 cysteine desulfurase SufS subfamily
Length = 419
Score = 81.3 bits (199), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 71/230 (30%), Positives = 109/230 (47%), Gaps = 8/230 (3%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
VYLD +AT+ +P+ + AI T +H +A E AR +V INA
Sbjct: 30 VYLDNAATS-QKPQVVLDAIQNYYQTYNSNVHRGIHTLSAKATDEYEKARQKVVEFINAD 88
Query: 61 P-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEITR 117
+ II+T +EA N+ +I+S +EH + P +ML + G I
Sbjct: 89 SYQEIIYTRNASEAVNVVAYSWGWENLRAGDEIILSVMEHHSNLVPWQMLAKKTGAVIKY 148
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
+G+ + E+ K + T LV+V+ + +G + P+ E+ +I +GA DA Q
Sbjct: 149 VGLTANQEFDFEEFKTLISPQTKLVAVLQMSNVLGCINPVKEITEIAHQYGAKVLIDACQ 208
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALY-LRPGVELM-PLLGGG 225
LP++V+ + D L S HK+ G G G LY R +E M P LGGG
Sbjct: 209 SLPHLPINVKEIDCDWLVGSGHKMCGPTGIGFLYGKREILESMSPFLGGG 258
>SPLC1_S533310 cysteine desulfurase SufS subfamily
Length = 625
Score = 68.6 bits (166), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 108/407 (26%), Positives = 169/407 (41%), Gaps = 58/407 (14%)
Query: 3 VYLDYSATTPTRPEAI--AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
++ D +ATT +P+A+ ++ + + HE R+ E AR V INA
Sbjct: 243 IWFDNAATT-QKPQAVIDRLVHYYSHENSNVHRAAHELAARSTDAYEAARETVRHFINAS 301
Query: 61 P-ESIIFTSGGTEADNLAIMGVARCY-----TTPQHMIISSVEHSAISEPAKMLENWGWE 114
IIF G TEA NL VA+ + +++S +EH A P + L
Sbjct: 302 SVNEIIFVRGTTEAINL----VAKSWGEQNIKAGDEIVLSHLEHHANIVPWQQLATRTGA 357
Query: 115 ITRLG-VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTH--GALF 171
R+ V+ +G++ E+ + L T LV+ + +GT+ P A++ I + H GA
Sbjct: 358 KLRIAPVDDRGQILLEEYQQLLNPRTKLVAFSQVSNALGTITPAAKM--IAQAHQVGAKV 415
Query: 172 HTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELM-----PLLGGGG 226
D Q +P+DVQ L D S HK++G G G +Y G E + P GGG
Sbjct: 416 LLDGAQSVSHIPVDVQQLGCDWFVFSGHKIFGPTGIGVVY---GHEDLLNASPPWQGGGN 472
Query: 227 QENGL---RSGTQATPAI--AGFGVAAE----LAAQELETETGRLILLR------DRLFA 271
+ R+ Q +PA AG G A+ A + + G + R +
Sbjct: 473 MIVDVTFERTVYQNSPARFEAGTGNIADAVGLATALDYVQQIGLPNIARYEHELLEYATT 532
Query: 272 ILADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGK 331
L +PGL G + LSF L+ E + + LN GI + AG C
Sbjct: 533 GLNTIPGLHLIGTAPEKA-AVLSFVLDGFSAEAVG-----QALNQEGIAVRAGHHCA--- 583
Query: 332 LSPSPILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQILQR 378
PIL G +R +L T +++D +++I Q+
Sbjct: 584 ---QPILRRFGLE-----ATVRPSLAFYNTKSEVDALVHALERIRQK 622
>SPLC1_S101340 aminotransferase class V
Length = 391
Score = 42.7 bits (99), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 45/173 (26%), Positives = 79/173 (45%), Gaps = 12/173 (6%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEH-SAISEPAKML 108
R +A + +I T T N+A+ G+ + H++IS EH ++ +++
Sbjct: 66 REAIASELGVQSATITLTEDVTVGCNIALWGID--WQRGDHLLISDCEHPGVVATVGELV 123
Query: 109 ENWGWEIT---RLGVNHQGRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKIT 164
+ E++ L ++G +P E + + ++ NT LV + + G V P+ + +
Sbjct: 124 RRFQIEVSVCPLLETLNEG--DPVEAIASRIKSNTRLVVISHILWNTGQVLPLTAIMQAC 181
Query: 165 K--THGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214
K + G DA Q G LPL++ VD + HK L G G G LY+ P
Sbjct: 182 KAASGGVRVLVDAAQSVGVLPLNLAESGVDFYGFTGHKWLCGPEGLGGLYVSP 234
>SPLC1_S208850 aminotransferase class V
Length = 98
Score = 33.9 bits (76), Expect = 0.004, Method: Composition-based stats.
Identities = 17/43 (39%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAAL 44
+YLDY ATT P I +T ++GN +S+ H +G+R L
Sbjct: 7 IYLDYHATTTVDPRVAEKILHYMTVEFGNANSIDHIYGDRETL 49
>SPLC1_S082020 hypothetical protein
Length = 173
Score = 28.5 bits (62), Expect = 0.59, Method: Compositional matrix adjust.
Identities = 18/58 (31%), Positives = 33/58 (56%), Gaps = 2/58 (3%)
Query: 114 EITRLGVNHQGRVNPED-LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGAL 170
E+ + + +GR NP+D LKA + N ++ + YGQ +I V+ + +T+ G+L
Sbjct: 53 EVDVIALGMRGR-NPQDFLKAGEKTNLIIRNQPYGQVDIKAVEFLPRTIPVTQPDGSL 109
Database: GCA_000307915.1P
Posted date: Aug 27, 2016 10:24 PM
Number of letters in database: 1,647,000
Number of sequences in database: 6108
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 6108
Number of Hits to DB: 1,277,530
Number of extensions: 53941
Number of successful extensions: 182
Number of sequences better than 1.0: 9
Number of HSP's gapped: 173
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 1,647,000
Length adjustment: 86
Effective length of query: 296
Effective length of database: 1,121,712
Effective search space: 332026752
Effective search space used: 332026752
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)