cyanobase / GCA_001277295.1 - AA650

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000284455.1P 3169 sequences; 1,031,404 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SYNPCCP_0100 nifS NifS protein 494 e-175 SYNPCCP_2143 nifS NifS protein 259 2e-83 SYNPCCP_2581 nifS NifS protein 88 4e-20 SYNPCCP_1148 cefD isopenicillin N epimerase 44 1e-05 SYNPCCP_1799 sll1559 soluble hydrogenase 42 kDa subunit 28 0.97 B72a58c45ca6895a574441516f2a9550

>SYNPCCP_0100 nifS NifS protein Length = 391 Score = 494 bits (1271), Expect = e-175, Method: Compositional matrix adjust. Identities = 245/385 (63%), Positives = 299/385 (77%), Gaps = 3/385 (0%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 M++YLDYSATTP R E A +Q+ L Q WGNP+SLH WGNRAAL LETAR+QVA L+NA Sbjct: 1 MKIYLDYSATTPPRQEVKAAVQSFLDQSWGNPASLHHWGNRAALALETARLQVAQLLNAS 60 Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 P+SI+FTSGGTEA++LAI GV R Y +PQH+IIS+VEHSAI+EP + LEN GW++TRLG Sbjct: 61 HPDSIVFTSGGTEANHLAIFGVTRNYPSPQHLIISTVEHSAIAEPVRWLENQGWQVTRLG 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 VN QGRVNP +L+ A++ NTVL+SVIYGQSE+GT+QPI ELG+I + FHTDAVQVA Sbjct: 121 VNAQGRVNPLELEQAIQSNTVLISVIYGQSEVGTLQPIEELGRIARQRHIPFHTDAVQVA 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 GR P+DV+ LPVDLLSLSSHKLYG GAGALY+ P + + PLL GGGQE GLRSGT P Sbjct: 181 GRCPVDVKRLPVDLLSLSSHKLYGIQGAGALYIHPDLAIGPLLMGGGQEQGLRSGTPPLP 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 AI G G+AA+LA EL E RL LRDR F +LAD L PTGD+++RLPHH+SF +++ Sbjct: 241 AIVGLGIAAKLAHHELAEEMTRLQTLRDRCFDLLADCEFLTPTGDRLYRLPHHVSFVVQS 300 Query: 300 AD--GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357 ++GK LVR LN GI ISAGAACNSGKL+PSP+LLAMGY ++ AL GIRLT G Sbjct: 301 RRHCTAGLTGKQLVRALNREGIAISAGAACNSGKLNPSPVLLAMGYQEQQALAGIRLTFG 360 Query: 358 KQTTAADIDWTGIVVKQILQRLIPD 382 ++TT ADI+ T +KQ+ +L P Sbjct: 361 RETTMADIETTARTIKQVFAQLCPQ 385 >SYNPCCP_2143 nifS NifS protein Length = 386 Score = 259 bits (663), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 150/383 (39%), Positives = 217/383 (56%), Gaps = 9/383 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +Y D ATT P + + LT+Q+GN S H +G +A+ ++ AR ++A + A PE Sbjct: 5 LYFDNHATTALDPRVLEAMLPYLTEQYGNAGSAHFYGWQASAAIKQARQEIAETMGAQPE 64 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121 IIFTSG TEA+NLAI GVA Y +H++ EH A+ P + LE G+E+T L V Sbjct: 65 EIIFTSGATEANNLAIRGVAEAYFAQGKHLVTVETEHQAVLAPCRYLETLGFEVTYLSVQ 124 Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181 G V+ +L+ ALR +T+LVSV+ +EIG +QP+ E+G + + +FH D Q G+ Sbjct: 125 SSGLVDLTELEKALRPDTILVSVMAANNEIGVLQPLKEIGALCRQRSIIFHCDGAQALGK 184 Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR--PGVELMPLLGGGGQENGLRSGTQATP 239 +PLDV L +DLLS + HK++G G G LY R PGV L P L GGGQE RSGT P Sbjct: 185 IPLDVHQLNIDLLSFTGHKIHGPKGIGGLYRRQNPGVRLAPQLLGGGQEGNFRSGTLPVP 244 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 I G A +A + L +E R LRD+L+ LA + G++ GD RLP +L+ + Sbjct: 245 LIVGLAKALTIAGETLVSEGDRQRQLRDQLWQGLAKISGVVLNGDYEQRLPGNLNVSITG 304 Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359 D K L+ L + +S+G+AC+S + S +L A+G + +A +R L + Sbjct: 305 VD-----PKALLTTLQ-PRLALSSGSACSSYRTEASHVLYALGRDKTSAQASLRFGLSRF 358 Query: 360 TTAADIDWTGIVVKQILQRLIPD 382 TT +ID +V + RL D Sbjct: 359 TTEIEIDQAIAIVTDTVARLRAD 381 >SYNPCCP_2581 nifS NifS protein Length = 420 Score = 88.2 bits (217), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 72/233 (30%), Positives = 111/233 (47%), Gaps = 11/233 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58 VYLD +AT+ +P A+ ++ ++ + +++H + RA E R +VA IN Sbjct: 30 VYLDNAATS-QKPRAV--LEKLMHYYENDNANVHRGAHQLSVRATDAYEAVRNKVAKFIN 86 Query: 59 A-VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKMLE-NWGWEI 115 A P I++T TEA NL II++V EH + P +M+ G + Sbjct: 87 ARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVMEHHSNLVPWQMVAAKTGAVL 146 Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 + ++ Q + E K L T LV+V++ + +G V P E+ ++ GA DA Sbjct: 147 KFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNPAEEIAQLAHQAGAKVLVDA 206 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227 Q A PLDVQ + D L S HK+ G G LY + + E MP GGG+ Sbjct: 207 CQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEILEAMPPFFGGGE 259 >SYNPCCP_1148 cefD isopenicillin N epimerase Length = 401 Score = 43.5 bits (101), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 43/177 (24%), Positives = 72/177 (40%), Gaps = 22/177 (12%) Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE 109 R + N P +I T T ++ + G+ + ++++ EH I + + Sbjct: 68 RQALGETFNVDPNTITITDNVTTGCDIVLWGID--WQKGDEILLTDCEHPGIIAIVQAIA 125 Query: 110 NWGWEITRLGVNHQ-----GRVNPEDLKAALRHN----TVLVSVIYGQSEIGTVQPIAEL 160 R GV ++ +N D A L ++ T LV + + G V P+ E+ Sbjct: 126 ------ARFGVTYRFFPVADTLNQGDAAAVLVNHLGPKTRLVILSHLLWNTGQVLPLGEI 179 Query: 161 GKITKTHGALFHT----DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYL 212 + + H + D Q AG LPLD L VD + + HK + G G G LY+ Sbjct: 180 MAVCRRHQGDYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYI 236 >SYNPCCP_1799 sll1559 soluble hydrogenase 42 kDa subunit Length = 384 Score = 27.7 bits (60), Expect = 0.97, Method: Compositional matrix adjust. Identities = 25/102 (24%), Positives = 47/102 (46%), Gaps = 5/102 (4%) Query: 106 KMLENWGWEITRLGVNHQGRVNPEDLKAALRHN---TVLVSVI-YGQSEIGTVQPIAELG 161 K+ + +G + + ++P D K L + T+ +I + ++ G + +A + Sbjct: 95 KVAKTFGLAVEEIKAEWGKALDPNDFKTLLEADSDKTIKALIITHSETSTGVLNDLAAIN 154 Query: 162 KITKTHG-ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLY 202 K HG AL DAV G P+ + L +D+++ S K Y Sbjct: 155 AAAKAHGGALMIVDAVTSLGATPVAIDDLGLDVVASGSQKGY 196 Database: GCA_000284455.1P Posted date: Aug 27, 2016 10:37 PM Number of letters in database: 1,031,404 Number of sequences in database: 3169 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3169 Number of Hits to DB: 811,894 Number of extensions: 35819 Number of successful extensions: 135 Number of sequences better than 1.0: 7 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 7 Length of query: 382 Length of database: 1,031,404 Length adjustment: 84 Effective length of query: 298 Effective length of database: 765,208 Effective search space: 228031984 Effective search space used: 228031984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)