BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000270265.1P
3171 sequences; 1,032,334 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SYNGTS_0100 nifS NifS protein 494 e-175
SYNGTS_2145 nifS NifS protein 259 2e-83
SYNGTS_2583 nifS NifS protein 88 4e-20
SYNGTS_1149 cefD isopenicillin N epimerase 44 1e-05
SYNGTS_1800 sll1559 soluble hydrogenase 42 kDa subunit 28 0.98
>SYNGTS_0100 nifS NifS protein
Length = 391
Score = 494 bits (1271), Expect = e-175, Method: Compositional matrix adjust.
Identities = 245/385 (63%), Positives = 299/385 (77%), Gaps = 3/385 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
M++YLDYSATTP R E A +Q+ L Q WGNP+SLH WGNRAAL LETAR+QVA L+NA
Sbjct: 1 MKIYLDYSATTPPRQEVKAAVQSFLDQSWGNPASLHHWGNRAALALETARLQVAQLLNAS 60
Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119
P+SI+FTSGGTEA++LAI GV R Y +PQH+IIS+VEHSAI+EP + LEN GW++TRLG
Sbjct: 61 HPDSIVFTSGGTEANHLAIFGVTRNYPSPQHLIISTVEHSAIAEPVRWLENQGWQVTRLG 120
Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179
VN QGRVNP +L+ A++ NTVL+SVIYGQSE+GT+QPI ELG+I + FHTDAVQVA
Sbjct: 121 VNAQGRVNPLELEQAIQSNTVLISVIYGQSEVGTLQPIEELGRIARQRHIPFHTDAVQVA 180
Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
GR P+DV+ LPVDLLSLSSHKLYG GAGALY+ P + + PLL GGGQE GLRSGT P
Sbjct: 181 GRCPVDVKRLPVDLLSLSSHKLYGIQGAGALYIHPDLAIGPLLMGGGQEQGLRSGTPPLP 240
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
AI G G+AA+LA EL E RL LRDR F +LAD L PTGD+++RLPHH+SF +++
Sbjct: 241 AIVGLGIAAKLAHHELAEEMTRLQTLRDRCFDLLADCEFLTPTGDRLYRLPHHVSFVVQS 300
Query: 300 AD--GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
++GK LVR LN GI ISAGAACNSGKL+PSP+LLAMGY ++ AL GIRLT G
Sbjct: 301 RRHCTAGLTGKQLVRALNREGIAISAGAACNSGKLNPSPVLLAMGYQEQQALAGIRLTFG 360
Query: 358 KQTTAADIDWTGIVVKQILQRLIPD 382
++TT ADI+ T +KQ+ +L P
Sbjct: 361 RETTMADIETTARTIKQVFAQLCPQ 385
>SYNGTS_2145 nifS NifS protein
Length = 386
Score = 259 bits (663), Expect = 2e-83, Method: Compositional matrix adjust.
Identities = 150/383 (39%), Positives = 217/383 (56%), Gaps = 9/383 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+Y D ATT P + + LT+Q+GN S H +G +A+ ++ AR ++A + A PE
Sbjct: 5 LYFDNHATTALDPRVLEAMLPYLTEQYGNAGSAHFYGWQASAAIKQARQEIAETMGAQPE 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
IIFTSG TEA+NLAI GVA Y +H++ EH A+ P + LE G+E+T L V
Sbjct: 65 EIIFTSGATEANNLAIRGVAEAYFAQGKHLVTVETEHQAVLAPCRYLETLGFEVTYLSVQ 124
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
G V+ +L+ ALR +T+LVSV+ +EIG +QP+ E+G + + +FH D Q G+
Sbjct: 125 SSGLVDLTELEKALRPDTILVSVMAANNEIGVLQPLKEIGALCRQRSIIFHCDGAQALGK 184
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR--PGVELMPLLGGGGQENGLRSGTQATP 239
+PLDV L +DLLS + HK++G G G LY R PGV L P L GGGQE RSGT P
Sbjct: 185 IPLDVHQLNIDLLSFTGHKIHGPKGIGGLYRRQNPGVRLAPQLLGGGQEGNFRSGTLPVP 244
Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299
I G A +A + L +E R LRD+L+ LA + G++ GD RLP +L+ +
Sbjct: 245 LIVGLAKALTIAGETLVSEGDRQRQLRDQLWQGLAKISGVVLNGDYEQRLPGNLNVSITG 304
Query: 300 ADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQ 359
D K L+ L + +S+G+AC+S + S +L A+G + +A +R L +
Sbjct: 305 VD-----PKALLTTLQ-PRLALSSGSACSSYRTEASHVLYALGRDKTSAQASLRFGLSRF 358
Query: 360 TTAADIDWTGIVVKQILQRLIPD 382
TT +ID +V + RL D
Sbjct: 359 TTEIEIDQAIAIVTDTVARLRAD 381
>SYNGTS_2583 nifS NifS protein
Length = 420
Score = 88.2 bits (217), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 72/233 (30%), Positives = 111/233 (47%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
VYLD +AT+ +P A+ ++ ++ + +++H + RA E R +VA IN
Sbjct: 30 VYLDNAATS-QKPRAV--LEKLMHYYENDNANVHRGAHQLSVRATDAYEAVRNKVAKFIN 86
Query: 59 A-VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKMLE-NWGWEI 115
A P I++T TEA NL II++V EH + P +M+ G +
Sbjct: 87 ARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVMEHHSNLVPWQMVAAKTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ ++ Q + E K L T LV+V++ + +G V P E+ ++ GA DA
Sbjct: 147 KFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNPAEEIAQLAHQAGAKVLVDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV-ELMPLLGGGGQ 227
Q A PLDVQ + D L S HK+ G G LY + + E MP GGG+
Sbjct: 207 CQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEILEAMPPFFGGGE 259
>SYNGTS_1149 cefD isopenicillin N epimerase
Length = 401
Score = 43.5 bits (101), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 43/177 (24%), Positives = 72/177 (40%), Gaps = 22/177 (12%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE 109
R + N P +I T T ++ + G+ + ++++ EH I + +
Sbjct: 68 RQALGETFNVDPNTITITDNVTTGCDIVLWGID--WQKGDEILLTDCEHPGIIAIVQAIA 125
Query: 110 NWGWEITRLGVNHQ-----GRVNPEDLKAALRHN----TVLVSVIYGQSEIGTVQPIAEL 160
R GV ++ +N D A L ++ T LV + + G V P+ E+
Sbjct: 126 ------ARFGVTYRFFPVADTLNQGDAAAVLVNHLGPKTRLVILSHLLWNTGQVLPLGEI 179
Query: 161 GKITKTHGALFHT----DAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYL 212
+ + H + D Q AG LPLD L VD + + HK + G G G LY+
Sbjct: 180 MAVCRRHQGDYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYI 236
>SYNGTS_1800 sll1559 soluble hydrogenase 42 kDa subunit
Length = 384
Score = 27.7 bits (60), Expect = 0.98, Method: Compositional matrix adjust.
Identities = 25/102 (24%), Positives = 47/102 (46%), Gaps = 5/102 (4%)
Query: 106 KMLENWGWEITRLGVNHQGRVNPEDLKAALRHN---TVLVSVI-YGQSEIGTVQPIAELG 161
K+ + +G + + ++P D K L + T+ +I + ++ G + +A +
Sbjct: 95 KVAKTFGLAVEEIKAEWGKALDPNDFKTLLEADSDKTIKALIITHSETSTGVLNDLAAIN 154
Query: 162 KITKTHG-ALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLY 202
K HG AL DAV G P+ + L +D+++ S K Y
Sbjct: 155 AAAKAHGGALMIVDAVTSLGATPVAIDDLGLDVVASGSQKGY 196
Database: GCA_000270265.1P
Posted date: Aug 27, 2016 11:13 PM
Number of letters in database: 1,032,334
Number of sequences in database: 3171
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3171
Number of Hits to DB: 812,573
Number of extensions: 35835
Number of successful extensions: 135
Number of sequences better than 1.0: 7
Number of HSP's gapped: 129
Number of HSP's successfully gapped: 7
Length of query: 382
Length of database: 1,032,334
Length adjustment: 84
Effective length of query: 298
Effective length of database: 765,970
Effective search space: 228259060
Effective search space used: 228259060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)