BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000235665.2P 6124 sequences; 1,573,874 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CWATWH0003_4429a1 Cysteine desulfurase 533 0.0 CWATWH0003_2896 Cysteine desulfurase 267 5e-85 CWATWH0003_1015 Cysteine desulfurase 134 9e-38 CWATWH0003_1016 Cysteine desulfurase 112 8e-30 CWATWH0003_0296 Cysteine desulfurase 97 4e-23 CWATWH0003_4172 Cysteine desulfurase 57 1e-09 CWATWH0003_1805 Serine--pyruvate aminotransferase / L-alanine:gl... 39 4e-04 CWATWH0003_5459t1 Pyridoxal-dependent decarboxylase 32 0.091 83d69b2f66832d04d97c15a0d111a3e0 >CWATWH0003_4429a1 Cysteine desulfurase Length = 388 Score = 533 bits (1373), Expect = 0.0, Method: Compositional matrix adjust. Identities = 260/386 (67%), Positives = 315/386 (81%), Gaps = 5/386 (1%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 MQ+YLDYSATTP R + ++ + IL ++WGNPSSLH+WG RAA+ +E AR QVA LINA Sbjct: 1 MQIYLDYSATTPPRSKVVSYVNQILREEWGNPSSLHQWGQRAAMTMEIARTQVASLINAN 60 Query: 61 -PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 PES+IFTSGGTEADNLAI+GVAR Y TPQH+IIS VEH AISEP + LE GWE+TRL Sbjct: 61 DPESVIFTSGGTEADNLAILGVARQYDTPQHLIISRVEHPAISEPVRYLEGLGWEVTRLP 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 VN QGR+NP DLKAA++ NTVL+S+I+GQSE+GTVQPI ELG I HG LFHTDAVQ A Sbjct: 121 VNRQGRINPLDLKAAIQSNTVLISIIHGQSEVGTVQPIEELGNIAHAHGILFHTDAVQSA 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 GR+ +DVQ LP+DLLSLS+HK+YG GAGALY+R G+++ PLL GGGQE RSGTQA Sbjct: 181 GRVTIDVQRLPIDLLSLSAHKIYGIQGAGALYVRNGLKIEPLLLGGGQEGKTRSGTQAVG 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL-- 297 IAGFG+AAELAA E+ E+ RLI LRDRLF ++A+ P L+PTGD+++RLPHH+SF + Sbjct: 241 MIAGFGLAAELAATEMIAESSRLIGLRDRLFDLMANCPYLVPTGDRLYRLPHHVSFVINY 300 Query: 298 --EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLT 355 E EKI+GK +VRQ+NLAGI ISAG+AC+SGKLSPSPILLAMGYS+K AL GIRLT Sbjct: 301 PFETDKTEKITGKMIVRQMNLAGIAISAGSACHSGKLSPSPILLAMGYSEKEALRGIRLT 360 Query: 356 LGKQTTAADIDWTGIVVKQILQRLIP 381 LG++TT DIDWT +V+KQ+L RL+P Sbjct: 361 LGRETTLEDIDWTAMVLKQVLNRLMP 386 >CWATWH0003_2896 Cysteine desulfurase Length = 469 Score = 267 bits (682), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 152/378 (40%), Positives = 212/378 (56%), Gaps = 7/378 (1%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +YLD +ATT E I + LT +GNPSS+H +G + A ++ AR QVA L+ A Sbjct: 5 IYLDNNATTKIDEEVIGAMMPYLTIYYGNPSSMHSFGGQVAKAVQKAREQVAALLGAEHS 64 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122 IIFTS GTE DN AI H+I + VEHSA+ K LE G+ +T LGV+ Sbjct: 65 EIIFTSCGTEGDNAAICAALTAQPDKHHIITTEVEHSAVLNFCKHLEKQGYSVTYLGVDS 124 Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182 QGR++ ++++AAL NT LV+++Y +E G + P+ E+GK+ K +GALFH DAVQ G++ Sbjct: 125 QGRLDLDEVQAALTGNTALVTMMYANNETGVIFPVEEVGKLAKEYGALFHVDAVQAVGKV 184 Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242 P+D+ +DLL+LS HKL+ G GALY R G+ + GG QE G R GT+ P I Sbjct: 185 PMDMSKSSIDLLTLSGHKLHAPKGVGALYARKGINFPAFMIGGHQERGRRGGTENVPGII 244 Query: 243 GFGVAAELAAQELETETGRLILLRDRL-FAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301 G A ELA LE + LRD L ++L + + G RL + + + + Sbjct: 245 ALGKACELAQSHLENLSHER-QLRDYLETSLLTLISDTVVNGTTEERLANTTNIAFKYVE 303 Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361 GE I + LN GI SAG+AC SG + PS +L AMG G IR +L + TT Sbjct: 304 GEGI-----LSSLNQYGICASAGSACTSGSMEPSHVLKAMGLPNSVLQGSIRFSLSRYTT 358 Query: 362 AADIDWTGIVVKQILQRL 379 +I+ +V I+ L Sbjct: 359 QEEINRVIEIVPDIISHL 376 >CWATWH0003_1015 Cysteine desulfurase Length = 188 Score = 134 bits (337), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 73/171 (42%), Positives = 103/171 (60%), Gaps = 2/171 (1%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLD ATTP + + T+ +GNPSS+ H +G A ++ AR +A IN P Sbjct: 6 IYLDCHATTPMDKRVLEAMLPYFTEHFGNPSSINHLYGWEAEAAVKKARETIANAINCSP 65 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E IIFTSG TEA+NLAI GVA Y + QH+I EH A+ +P K LE G+EIT L V Sbjct: 66 EEIIFTSGATEANNLAIKGVAEAYFSQGQHIITVESEHRAVLDPCKYLEILGFEITYLPV 125 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALF 171 G ++ E L+ ++R+ T+LVSV+ +EIG +QP+ +G+I + F Sbjct: 126 QKDGLIDLELLEKSIRNETILVSVMTANNEIGVLQPLQAIGEICQKKMFYF 176 >CWATWH0003_1016 Cysteine desulfurase Length = 195 Score = 112 bits (281), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 83/197 (42%), Positives = 113/197 (57%), Gaps = 17/197 (8%) Query: 189 LPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQATPAIAGFG 245 + ++LLSL++HK+YG G GALY+R P V + P L GGGQE +RSGT TP I GFG Sbjct: 1 MNINLLSLTAHKVYGPKGIGALYIRRRDPRVNIAPQLHGGGQEKEIRSGTLYTPQIVGFG 60 Query: 246 VAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAADGEKI 305 A EL+ +ELETE R LRDRL L + G+ G RL +++ +E DG + Sbjct: 61 KAIELSLEELETENKRQTELRDRLQKQLLSLEGVHLNGHPTQRLAGNINVSIEGVDGAAL 120 Query: 306 SGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADI 365 L Q N+A +S+G+AC S PS +L A+G+S K A +R LG+ TT +I Sbjct: 121 ---LLALQGNVA---LSSGSACTSTTTKPSHVLKALGHSDKLAYSSLRFGLGRFTTCQEI 174 Query: 366 DWTGIVVKQILQRLIPD 382 D I+ +LI D Sbjct: 175 D--------IISKLIID 183 >CWATWH0003_0296 Cysteine desulfurase Length = 420 Score = 97.1 bits (240), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 113/404 (27%), Positives = 170/404 (42%), Gaps = 49/404 (12%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61 VYLD +AT+ + +Q + N H RA E +R ++A INA Sbjct: 30 VYLDNAATSQKPVTVLDALQNYYGKDNANVHRGAHSLSIRATEAYEGSRDKIAQFINAAS 89 Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKML-ENWGWEITRL 118 + +IFT TEA N+ + P II+SV EH + P +M+ + G I + Sbjct: 90 RQEVIFTRNATEAINVVAYSWGLHHLQPGDEIITSVMEHHSNFVPWQMIAKQTGAVIKYV 149 Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178 + + E+ K+ L T LV++++ + +G + P+ E+ K+ G DA Q Sbjct: 150 PLTDNEDFDLEEFKSLLSEKTKLVTIVHVSNTLGCINPVEEVIKLAHEKGVKVLIDACQS 209 Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGGQENGL----- 231 + +DVQ + D L S HK+ G G G LY + + E+ P LGGG N + Sbjct: 210 VPHMAIDVQAMDCDWLVASGHKMCGPSGVGFLYGKQEILEEMPPFLGGGEMINEVFFDSF 269 Query: 232 ------RSGTQATPAIAGFGVAAEL-AAQELETETGRLIL------LRDRLFAILADVPG 278 TPAI G A L AA + T+ G + L LF LAD+P Sbjct: 270 TCGELPHKFEAGTPAI---GEAIALGAAVDYLTDIGMDKIHACEEELTAYLFRKLADIPN 326 Query: 279 L-----IPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLS 333 L +PT + R L A + E I G L L+ G+ I +G C Sbjct: 327 LKIYGPLPTPEGKGRAA------LAAFNIEGIHGSDLSTLLDQEGVAIRSGHHCT----Q 376 Query: 334 PSPILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQILQ 377 P L A G R++L T +ID +V+K+ + Sbjct: 377 PLHRLF-------DASGSARVSLYFYNTYDEIDQFVMVLKETID 413 >CWATWH0003_4172 Cysteine desulfurase Length = 399 Score = 56.6 bits (135), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 62/234 (26%), Positives = 103/234 (44%), Gaps = 19/234 (8%) Query: 2 QVYLDY--SATTPTRP-EAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLIN 58 +VY ++ T P EAI L QQ +++W + +L R +A + Sbjct: 24 KVYFNFGGQGTLPRSGLEAIIDAHNFLQQQGPFSLKVNDWITQKTELL---RQDIAQELG 80 Query: 59 AVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAI-SEPAKMLENWGWEITR 117 +I T T N+A+ G+ + ++I+ EH I + ++ + +G EI Sbjct: 81 VEASTITLTENVTAGCNIALWGID--WQEGDRILITDCEHPGIIATVQEIAKRFGVEIDI 138 Query: 118 LGV-NHQGRVNP-EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA-----L 170 + N +P E +K L+ T L+ + + G + P+ E+ I + L Sbjct: 139 CPILNTLNEGDPVEVIKHHLKTETKLLVLSHLLWNTGQILPLQEISNICHNYSGSDRPIL 198 Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPGVELMPLLG 223 DA Q AG LPL++ VD + + HK + G G GALY+RP E+ LL Sbjct: 199 VLADAAQSAGSLPLNLAETGVDFYAFTGHKWFCGPAGVGALYIRP--EIFELLN 250 >CWATWH0003_1805 Serine--pyruvate aminotransferase / L-alanine:glyoxylate aminotransferase Length = 372 Score = 39.3 bits (90), Expect = 4e-04, Method: Compositional matrix adjust. Identities = 24/79 (30%), Positives = 38/79 (48%), Gaps = 4/79 (5%) Query: 130 DLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQT 188 +LK AL H +++++ ++ G QP+ +G++ + H L DAV G +P Sbjct: 130 ELKEALETHKPAILALVNAETSTGVRQPLEGVGELCREHNCLLLVDAVTSLGGVPFYADQ 189 Query: 189 LPVDLLSLSSHKLYGGLGA 207 VDL S K GLG Sbjct: 190 WGVDLAYSCSQK---GLGC 205 >CWATWH0003_5459t1 Pyridoxal-dependent decarboxylase Length = 553 Score = 32.0 bits (71), Expect = 0.091, Method: Compositional matrix adjust. Identities = 23/94 (24%), Positives = 48/94 (51%), Gaps = 8/94 (8%) Query: 89 QHMII--SSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAALRH---NTVLVS 143 + M+I S + H ++++ A +L I + N +G++N + L+ ++ N + + Sbjct: 233 EDMVIIGSRLMHYSLNKAASILGLGTDNIIFVDNNSEGKLNCKQLEEKIQECQENRLFIL 292 Query: 144 V---IYGQSEIGTVQPIAELGKITKTHGALFHTD 174 I G +E G + P+ +LG+I + + FH D Sbjct: 293 ALIGIAGTTETGQIDPLWKLGEIAEKYNIHFHVD 326 Database: GCA_000235665.2P Posted date: Aug 27, 2016 10:28 PM Number of letters in database: 1,573,874 Number of sequences in database: 6124 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 6124 Number of Hits to DB: 1,155,359 Number of extensions: 46894 Number of successful extensions: 128 Number of sequences better than 1.0: 8 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 8 Length of query: 382 Length of database: 1,573,874 Length adjustment: 86 Effective length of query: 296 Effective length of database: 1,047,210 Effective search space: 309974160 Effective search space used: 309974160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.6 bits)
0.868348417