BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000196515.1P
3651 sequences; 943,585 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Aazo_4811 Cysteine desulfurase 646 0.0
Aazo_3449 Cysteine desulfurase 285 3e-93
Aazo_1356 cysteine desulfurase NifS 284 1e-92
Aazo_0799 cysteine desulfurase, SufS subfamily 84 7e-19
Aazo_3645 cysteine desulfurase family protein 67 3e-13
Aazo_4300 conserved hypothetical protein 26 0.81
>Aazo_4811 Cysteine desulfurase
Length = 398
Score = 646 bits (1667), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 324/382 (84%), Positives = 352/382 (92%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTRPEAIA +Q +L QQWGNPSSLHEWGNRAAL++E AR+QVAGLINAV
Sbjct: 1 MQIYLDYSATTPTRPEAIATMQAVLNQQWGNPSSLHEWGNRAALVVEQARIQVAGLINAV 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
PESIIFTSGGTEADNLA+MGVARCY PQH+IISSVEHSA+SEP +MLENWGWE+TRLGV
Sbjct: 61 PESIIFTSGGTEADNLAVMGVARCYPVPQHIIISSVEHSAVSEPVRMLENWGWEVTRLGV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ +GR+NPEDLKAAL+HNTVLVSVIYGQSE+GTVQPIAELG+ITK HGALFHTDAVQVAG
Sbjct: 121 DGKGRINPEDLKAALQHNTVLVSVIYGQSEVGTVQPIAELGRITKIHGALFHTDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
RL +DV L +DLLSLSSHK+YG LGAGALY+RPG+ L+PLLGGGGQE GLRSGTQATPA
Sbjct: 181 RLAIDVNNLGIDLLSLSSHKIYGPLGAGALYVRPGMNLIPLLGGGGQEQGLRSGTQATPA 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFGVAAELA QELETE RL LRDRLF LAD+P LIPTGD+IHRLPHHLSF LE A
Sbjct: 241 IAGFGVAAELAGQELETERLRLTELRDRLFTKLADIPSLIPTGDRIHRLPHHLSFSLEYA 300
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQ AALGGIRLTLGKQT
Sbjct: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQIAALGGIRLTLGKQT 360
Query: 361 TAADIDWTGIVVKQILQRLIPD 382
TAAD+DWT IV+KQ+LQRL D
Sbjct: 361 TAADVDWTAIVLKQVLQRLTAD 382
>Aazo_3449 Cysteine desulfurase
Length = 389
Score = 285 bits (729), Expect = 3e-93, Method: Compositional matrix adjust.
Identities = 155/369 (42%), Positives = 229/369 (62%), Gaps = 11/369 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP + + T+++GNP+S+ H +G + ++ +R +A INA P
Sbjct: 6 IYLDCHATTPIDERVLNAMIPYFTEKFGNPASISHVYGWESEAAVKQSRDILATAINANP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLAI GVA Y QH++ + EH A+ EP + LE+ G+EI L V
Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFAKGQHIVTVATEHKAVLEPCEYLESMGFEIMVLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
N G ++ E L+ LRH+T+LVSV+ +EIG +QP+ ++GK+ + +FHTDA Q G
Sbjct: 126 NQDGLIDLEQLEKTLRHDTILVSVMAANNEIGVLQPLDKIGKMCRQKEIIFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PLDV+ L +DL+SL++HK+YG G GALY+R P ++L GGG E G+RSGT
Sbjct: 186 KIPLDVEALNIDLMSLTAHKVYGPKGIGALYVRRRNPRIKLAAQQHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A E+A E ETE RL LR+RL+ L+ + G+ G RL +L+ L
Sbjct: 246 TPQIVGFAKAVEIALAEQETENQRLTELRERLWKQLSTLEGIYINGHPQKRLAGNLNISL 305
Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
E DG +S L Q + +S+G+AC+S ++PS +L+A+G+ +K A +R +G
Sbjct: 306 EGVDGAALS---LALQ---PMVAVSSGSACSSNNVAPSYVLIALGHPEKLAYASVRFGMG 359
Query: 358 KQTTAADID 366
+ T +ID
Sbjct: 360 RFNTVEEID 368
>Aazo_1356 cysteine desulfurase NifS
Length = 402
Score = 284 bits (726), Expect = 1e-92, Method: Compositional matrix adjust.
Identities = 159/378 (42%), Positives = 226/378 (59%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P+ I I LT + NPSS+H +G + A ++ AR Q+A LI A
Sbjct: 6 IYLDNNATTKVAPQVIEAIMPFLTDYYANPSSMHTFGGQLAKSVKVAREQIAALIGAEES 65
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I S VEH A+ K LE G+++T L VN
Sbjct: 66 EIVFTSCGTEGDNTAIRAALLAQPEKRHIITSQVEHPAVLNVCKQLETQGYQVTYLSVND 125
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G+++ +L+A+L NT LV+V+Y +EIGT+ PI E+G K +GA+FH DAVQ G++
Sbjct: 126 KGQIDLNELEASLTGNTALVTVMYANNEIGTIFPIEEIGARVKEYGAIFHVDAVQAVGKV 185
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++T +D+L++S HK++ G GALY++ GV P L GG QE G R+GT+ P I
Sbjct: 186 PLNMKTSTIDMLTMSGHKIHAPKGIGALYVKRGVRFRPFLIGGHQERGRRAGTENVPGII 245
Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G A EL LE T R LRDRL +LA++P + GD +RLP+ + + +
Sbjct: 246 ALGKAGELEMLLLEEATKRERKLRDRLEQTLLANIPDCVVNGDVKNRLPNTSNIGFKYIE 305
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + LN GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 306 GEAI-----LLLLNKHGICASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLSRYTT 360
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID ++ +I++RL
Sbjct: 361 EAEIDQVIAIMPEIVERL 378
>Aazo_0799 cysteine desulfurase, SufS subfamily
Length = 423
Score = 84.3 bits (207), Expect = 7e-19, Method: Compositional matrix adjust.
Identities = 68/233 (29%), Positives = 112/233 (48%), Gaps = 11/233 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58
VYLD +AT+ +P A+ +Q+ S++H + +A E AR +VA IN
Sbjct: 30 VYLDNAATS-QKPLAVLNAWRDYYEQYN--SNVHRGAHFLSAKATDAYEAARDKVANFIN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEI 115
A + I++T +EA NL P +I+S +EH + P + + + G +
Sbjct: 87 AKSRQDIVYTRNASEAINLVAYSWGMKNLQPGDEIILSVMEHHSNIVPWQFVAQKTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + + E K + T LVSV++ + +G + P+ E+ +I + A F DA
Sbjct: 147 KFVELTPEETFDLEQFKKLISEKTKLVSVVHISNTLGCINPVKEIAEIAHRYDAKFLMDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR-PGVELMPLLGGGGQ 227
Q +P+DVQ + D L S HK+ G G LY + +E MP GGG+
Sbjct: 207 CQSVPHMPIDVQEINCDWLVASGHKMCAPTGIGFLYGKLELLEAMPPFFGGGE 259
>Aazo_3645 cysteine desulfurase family protein
Length = 451
Score = 67.4 bits (163), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 42/164 (25%), Positives = 75/164 (45%), Gaps = 1/164 (0%)
Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100
R ++ +AR A + + ++F + T + R +I++ ++H+A
Sbjct: 64 RTDAVINSARAASADFLGCDNDEVVFGANMTTLTFSVSRAIGRELQPGDQIIVTKLDHAA 123
Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159
P LE G I + +N ++ DL A + T LV+V Y + +GT+ IA+
Sbjct: 124 NISPWSALEEKGVNIQVVDINVADCTLDLNDLAAKINSRTKLVAVTYASNAVGTINDIAK 183
Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203
+ K+ GAL DAV + P++V L D L S++K +
Sbjct: 184 IVKLAHAVGALVFVDAVHYSPHAPMNVHHLDCDFLVCSAYKFFA 227
>Aazo_4300 conserved hypothetical protein
Length = 62
Score = 25.8 bits (55), Expect = 0.81, Method: Compositional matrix adjust.
Identities = 14/43 (32%), Positives = 24/43 (55%)
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVEL 218
+ G+L +D + + L LSSH L GG+G+G + G ++
Sbjct: 20 IHAPGKLLVDNISNRIALRPLSSHLLLGGIGSGKTTQKRGTKI 62
Database: GCA_000196515.1P
Posted date: Aug 27, 2016 11:12 PM
Number of letters in database: 943,585
Number of sequences in database: 3651
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3651
Number of Hits to DB: 716,455
Number of extensions: 30032
Number of successful extensions: 98
Number of sequences better than 1.0: 9
Number of HSP's gapped: 91
Number of HSP's successfully gapped: 9
Length of query: 382
Length of database: 943,585
Length adjustment: 82
Effective length of query: 300
Effective length of database: 644,203
Effective search space: 193260900
Effective search space used: 193260900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)