BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000196515.1P 3651 sequences; 943,585 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Aazo_4811 Cysteine desulfurase 646 0.0 Aazo_3449 Cysteine desulfurase 285 3e-93 Aazo_1356 cysteine desulfurase NifS 284 1e-92 Aazo_0799 cysteine desulfurase, SufS subfamily 84 7e-19 Aazo_3645 cysteine desulfurase family protein 67 3e-13 Aazo_4300 conserved hypothetical protein 26 0.81 17d8b0ae0a8874dbf6f131fc793d28b6 >Aazo_4811 Cysteine desulfurase Length = 398 Score = 646 bits (1667), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/382 (84%), Positives = 352/382 (92%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 MQ+YLDYSATTPTRPEAIA +Q +L QQWGNPSSLHEWGNRAAL++E AR+QVAGLINAV Sbjct: 1 MQIYLDYSATTPTRPEAIATMQAVLNQQWGNPSSLHEWGNRAALVVEQARIQVAGLINAV 60 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 PESIIFTSGGTEADNLA+MGVARCY PQH+IISSVEHSA+SEP +MLENWGWE+TRLGV Sbjct: 61 PESIIFTSGGTEADNLAVMGVARCYPVPQHIIISSVEHSAVSEPVRMLENWGWEVTRLGV 120 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 + +GR+NPEDLKAAL+HNTVLVSVIYGQSE+GTVQPIAELG+ITK HGALFHTDAVQVAG Sbjct: 121 DGKGRINPEDLKAALQHNTVLVSVIYGQSEVGTVQPIAELGRITKIHGALFHTDAVQVAG 180 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240 RL +DV L +DLLSLSSHK+YG LGAGALY+RPG+ L+PLLGGGGQE GLRSGTQATPA Sbjct: 181 RLAIDVNNLGIDLLSLSSHKIYGPLGAGALYVRPGMNLIPLLGGGGQEQGLRSGTQATPA 240 Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300 IAGFGVAAELA QELETE RL LRDRLF LAD+P LIPTGD+IHRLPHHLSF LE A Sbjct: 241 IAGFGVAAELAGQELETERLRLTELRDRLFTKLADIPSLIPTGDRIHRLPHHLSFSLEYA 300 Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQ AALGGIRLTLGKQT Sbjct: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQIAALGGIRLTLGKQT 360 Query: 361 TAADIDWTGIVVKQILQRLIPD 382 TAAD+DWT IV+KQ+LQRL D Sbjct: 361 TAADVDWTAIVLKQVLQRLTAD 382 >Aazo_3449 Cysteine desulfurase Length = 389 Score = 285 bits (729), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 155/369 (42%), Positives = 229/369 (62%), Gaps = 11/369 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLD ATTP + + T+++GNP+S+ H +G + ++ +R +A INA P Sbjct: 6 IYLDCHATTPIDERVLNAMIPYFTEKFGNPASISHVYGWESEAAVKQSRDILATAINANP 65 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E I+FTSG TEA+NLAI GVA Y QH++ + EH A+ EP + LE+ G+EI L V Sbjct: 66 EEIVFTSGATEANNLAIKGVAEAYFAKGQHIVTVATEHKAVLEPCEYLESMGFEIMVLPV 125 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 N G ++ E L+ LRH+T+LVSV+ +EIG +QP+ ++GK+ + +FHTDA Q G Sbjct: 126 NQDGLIDLEQLEKTLRHDTILVSVMAANNEIGVLQPLDKIGKMCRQKEIIFHTDAAQAIG 185 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237 ++PLDV+ L +DL+SL++HK+YG G GALY+R P ++L GGG E G+RSGT Sbjct: 186 KIPLDVEALNIDLMSLTAHKVYGPKGIGALYVRRRNPRIKLAAQQHGGGHERGMRSGTLY 245 Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297 TP I GF A E+A E ETE RL LR+RL+ L+ + G+ G RL +L+ L Sbjct: 246 TPQIVGFAKAVEIALAEQETENQRLTELRERLWKQLSTLEGIYINGHPQKRLAGNLNISL 305 Query: 298 EAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357 E DG +S L Q + +S+G+AC+S ++PS +L+A+G+ +K A +R +G Sbjct: 306 EGVDGAALS---LALQ---PMVAVSSGSACSSNNVAPSYVLIALGHPEKLAYASVRFGMG 359 Query: 358 KQTTAADID 366 + T +ID Sbjct: 360 RFNTVEEID 368 >Aazo_1356 cysteine desulfurase NifS Length = 402 Score = 284 bits (726), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 159/378 (42%), Positives = 226/378 (59%), Gaps = 6/378 (1%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +YLD +ATT P+ I I LT + NPSS+H +G + A ++ AR Q+A LI A Sbjct: 6 IYLDNNATTKVAPQVIEAIMPFLTDYYANPSSMHTFGGQLAKSVKVAREQIAALIGAEES 65 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122 I+FTS GTE DN AI +H+I S VEH A+ K LE G+++T L VN Sbjct: 66 EIVFTSCGTEGDNTAIRAALLAQPEKRHIITSQVEHPAVLNVCKQLETQGYQVTYLSVND 125 Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182 +G+++ +L+A+L NT LV+V+Y +EIGT+ PI E+G K +GA+FH DAVQ G++ Sbjct: 126 KGQIDLNELEASLTGNTALVTVMYANNEIGTIFPIEEIGARVKEYGAIFHVDAVQAVGKV 185 Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242 PL+++T +D+L++S HK++ G GALY++ GV P L GG QE G R+GT+ P I Sbjct: 186 PLNMKTSTIDMLTMSGHKIHAPKGIGALYVKRGVRFRPFLIGGHQERGRRAGTENVPGII 245 Query: 243 GFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301 G A EL LE T R LRDRL +LA++P + GD +RLP+ + + + Sbjct: 246 ALGKAGELEMLLLEEATKRERKLRDRLEQTLLANIPDCVVNGDVKNRLPNTSNIGFKYIE 305 Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361 GE I + LN GI S+G+AC SG L PS +L AMG G IR +L + TT Sbjct: 306 GEAI-----LLLLNKHGICASSGSACTSGSLEPSHVLRAMGLPYTTLHGSIRFSLSRYTT 360 Query: 362 AADIDWTGIVVKQILQRL 379 A+ID ++ +I++RL Sbjct: 361 EAEIDQVIAIMPEIVERL 378 >Aazo_0799 cysteine desulfurase, SufS subfamily Length = 423 Score = 84.3 bits (207), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 68/233 (29%), Positives = 112/233 (48%), Gaps = 11/233 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGN----RAALILETARMQVAGLIN 58 VYLD +AT+ +P A+ +Q+ S++H + +A E AR +VA IN Sbjct: 30 VYLDNAATS-QKPLAVLNAWRDYYEQYN--SNVHRGAHFLSAKATDAYEAARDKVANFIN 86 Query: 59 AVP-ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEI 115 A + I++T +EA NL P +I+S +EH + P + + + G + Sbjct: 87 AKSRQDIVYTRNASEAINLVAYSWGMKNLQPGDEIILSVMEHHSNIVPWQFVAQKTGAVL 146 Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 + + + + E K + T LVSV++ + +G + P+ E+ +I + A F DA Sbjct: 147 KFVELTPEETFDLEQFKKLISEKTKLVSVVHISNTLGCINPVKEIAEIAHRYDAKFLMDA 206 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR-PGVELMPLLGGGGQ 227 Q +P+DVQ + D L S HK+ G G LY + +E MP GGG+ Sbjct: 207 CQSVPHMPIDVQEINCDWLVASGHKMCAPTGIGFLYGKLELLEAMPPFFGGGE 259 >Aazo_3645 cysteine desulfurase family protein Length = 451 Score = 67.4 bits (163), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 42/164 (25%), Positives = 75/164 (45%), Gaps = 1/164 (0%) Query: 41 RAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSA 100 R ++ +AR A + + ++F + T + R +I++ ++H+A Sbjct: 64 RTDAVINSARAASADFLGCDNDEVVFGANMTTLTFSVSRAIGRELQPGDQIIVTKLDHAA 123 Query: 101 ISEPAKMLENWGWEITRLGVN-HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAE 159 P LE G I + +N ++ DL A + T LV+V Y + +GT+ IA+ Sbjct: 124 NISPWSALEEKGVNIQVVDINVADCTLDLNDLAAKINSRTKLVAVTYASNAVGTINDIAK 183 Query: 160 LGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG 203 + K+ GAL DAV + P++V L D L S++K + Sbjct: 184 IVKLAHAVGALVFVDAVHYSPHAPMNVHHLDCDFLVCSAYKFFA 227 >Aazo_4300 conserved hypothetical protein Length = 62 Score = 25.8 bits (55), Expect = 0.81, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 24/43 (55%) Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVEL 218 + G+L +D + + L LSSH L GG+G+G + G ++ Sbjct: 20 IHAPGKLLVDNISNRIALRPLSSHLLLGGIGSGKTTQKRGTKI 62 Database: GCA_000196515.1P Posted date: Aug 27, 2016 11:12 PM Number of letters in database: 943,585 Number of sequences in database: 3651 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3651 Number of Hits to DB: 716,455 Number of extensions: 30032 Number of successful extensions: 98 Number of sequences better than 1.0: 9 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 9 Length of query: 382 Length of database: 943,585 Length adjustment: 82 Effective length of query: 300 Effective length of database: 644,203 Effective search space: 193260900 Effective search space used: 193260900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
0.870253944