BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000180455.1P
5822 sequences; 1,812,051 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
OSCI_2510001 aromatic amino acid beta-eliminating lyase/threonin... 514 0.0
OSCI_1050002 aminotransferase, class V 278 5e-90
OSCI_3510013 nifS aminotransferase, class V, putative cysteine d... 273 4e-88
OSCI_340005 cysteine desulfurase 79 7e-17
OSCI_590009 aminotransferase, class V 48 8e-07
>OSCI_2510001 aromatic amino acid beta-eliminating lyase/threonine aldolase
Length = 367
Score = 514 bits (1323), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 250/361 (69%), Positives = 296/361 (81%), Gaps = 1/361 (0%)
Query: 21 IQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMG 80
+Q TQ WGNPSSLH+WG RAA LE +RMQVA LINA PES+IFTSGGTEADNLAI+G
Sbjct: 1 MQAAFTQYWGNPSSLHDWGQRAATTLELSRMQVASLINAPPESVIFTSGGTEADNLAILG 60
Query: 81 VARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAALRHNTV 140
+AR Y PQH+IISSVEH A++EP + LE GWE+TRL V+ GRV+P DL A+L+ NTV
Sbjct: 61 IARHYKQPQHIIISSVEHPAVAEPVRFLEQLGWEVTRLPVDRLGRVHPLDLHASLQPNTV 120
Query: 141 LVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK 200
LVSVI+GQSE+GT+QPI LGKI ++HG LFHTDAVQVAGR+P+DVQ LPVDLLS+SSHK
Sbjct: 121 LVSVIFGQSEVGTLQPIEALGKIARSHGVLFHTDAVQVAGRIPIDVQKLPVDLLSISSHK 180
Query: 201 LYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIAGFGVAAELAAQELETETG 260
+YG G+G LY+R GVEL P+ GGGGQE LRSGTQA P I GFGVAAELA QE+ ET
Sbjct: 181 IYGPQGSGMLYIREGVELAPVFGGGGQEMKLRSGTQAVPNIVGFGVAAELALQEMAEETP 240
Query: 261 RLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIG 320
RLI LRDRLF LA+VP L+PTGD+ RLPHHLSF + + D E I+GKTLVRQLNLAGIG
Sbjct: 241 RLIGLRDRLFDKLAEVPNLVPTGDRYCRLPHHLSFVVASKD-ENITGKTLVRQLNLAGIG 299
Query: 321 ISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQILQRLI 380
IS+G+AC+SGK++P P LLAMGYS+ A+ GIRLTLG+ TT ADIDWT I + QI++RL+
Sbjct: 300 ISSGSACSSGKITPIPTLLAMGYSETEAIRGIRLTLGRDTTEADIDWTAIAIAQIMERLM 359
Query: 381 P 381
P
Sbjct: 360 P 360
>OSCI_1050002 aminotransferase, class V
Length = 389
Score = 278 bits (710), Expect = 5e-90, Method: Compositional matrix adjust.
Identities = 149/373 (39%), Positives = 224/373 (60%), Gaps = 19/373 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD ATTP +A + + +GNP+S+ H +G A ++ +R +A INA P
Sbjct: 6 IYLDNHATTPIDERVLAAMLPYFREHFGNPASINHTYGWEAEAAVKQSRQAIADAINASP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
E I+FTSG TEA+NLA+ G+A Y +H+I + EH+A+ +P LE G+EIT L V
Sbjct: 66 EEIVFTSGATEANNLAVKGIAEAYCNKGRHIITVATEHNAVLDPCHYLEKLGFEITFLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ +L +R +T+LVSV+ +EIG +QPI E+ I + FHTDA Q G
Sbjct: 126 QSDGLIDLGNLARCIRADTILVSVMAANNEIGVIQPIGEIAAICRGADVFFHTDAAQAIG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PL+V+ L +DL+SL++HK+YG G GALY+R P V+L + GGG E G+RSGT
Sbjct: 186 KIPLNVEALKIDLMSLTAHKIYGPKGIGALYVRRRHPRVQLAAQMHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GF A ELA E++ E R+ LR RL+ L+ + ++ G IHRLP +L+ +
Sbjct: 246 TPQIVGFAKAVELAISEMDLEVERVTNLRQRLWDYLSPLGDIVINGHPIHRLPGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAG----IGISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
+ DG+ + L G + +S+G+AC S K++PS +L A+G +K A +R
Sbjct: 306 DGVDGQAL----------LLGVQPIVAVSSGSACTSAKIAPSHVLEAIGRKEKLAYASLR 355
Query: 354 LTLGKQTTAADID 366
+G+ +T +ID
Sbjct: 356 FGIGRFSTVEEID 368
>OSCI_3510013 nifS aminotransferase, class V, putative cysteine desulfurase
Length = 402
Score = 273 bits (698), Expect = 4e-88, Method: Compositional matrix adjust.
Identities = 155/378 (41%), Positives = 221/378 (58%), Gaps = 6/378 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT P + + LT+ +GNPSS+H +G + ++ AR QVAGL+ A
Sbjct: 5 IYLDNNATTKVDPGVLEAMLPYLTEYYGNPSSMHTFGGKVGKAVKQARAQVAGLLGADDS 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS G+E++N AI +H+I + VEH+A+ K LE G+ +T L V+
Sbjct: 65 EIVFTSCGSESNNTAIRAALAAQPDRRHIITTQVEHAAVINVCKQLEKQGYTVTYLSVDR 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG+++ +L+AAL NT LVS +Y +E GT+ PI ++G + K +GA+FH DAVQ G++
Sbjct: 125 QGQLDLMELEAALTGNTALVSTMYANNESGTIFPIEKIGALAKEYGAVFHVDAVQAVGKI 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL+++ +DLL+LS HKL+ G GALY+R G PLL GG QE G R+GT+ I
Sbjct: 185 PLNMKESTIDLLTLSGHKLHAPKGIGALYVRRGFHFRPLLIGGHQERGRRAGTENVAGII 244
Query: 243 GFGVAAELAAQELETETGRLILLRDRLFA-ILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G G AAEL + R LRDRL +L +P GD HRLP+ + + +
Sbjct: 245 GLGKAAELELLHMPEAIQRESKLRDRLEENLLLTIPNCEVNGDPAHRLPNTTNIGFKYIE 304
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
GE I + L+ GI S+G+AC SG L PS +L AMG G IR +L + TT
Sbjct: 305 GEAI-----LLSLDRYGICASSGSACTSGSLEPSHVLRAMGLPYVILHGSIRFSLSRYTT 359
Query: 362 AADIDWTGIVVKQILQRL 379
A+ID V+ I++RL
Sbjct: 360 DAEIDRVLTVMPGIVERL 377
>OSCI_340005 cysteine desulfurase
Length = 419
Score = 79.3 bits (194), Expect = 7e-17, Method: Compositional matrix adjust.
Identities = 64/229 (27%), Positives = 103/229 (44%), Gaps = 6/229 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
VYLD +AT+ + +++ Q N H RA E AR ++A INA
Sbjct: 30 VYLDNAATSQKPLAVLNVLRDYYQQDNSNVHRGAHTLSGRATDAYEAARDKIAAFINAAS 89
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKML-ENWGWEITRL 118
+ II+T +EA NL P +I+S +EH + P + + + G + +
Sbjct: 90 RQEIIYTRNASEAINLVAYSWGFSNLEPGDEIILSVMEHHSNIVPWQFVAKRTGAVLKFV 149
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
+ + E K + T LVSV++ + +G + P+ E+ +I +GA D Q
Sbjct: 150 ELTDTQEFDLELFKKLISDKTKLVSVVHVSNTLGCINPVQEICQIAHHYGAKVLIDGCQS 209
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
L +DVQ + D L S HK+ G G LY + + + P +GGG
Sbjct: 210 LPHLSVDVQEIDCDWLVASGHKMCAPTGIGFLYGKLDLLRSMPPFMGGG 258
>OSCI_590009 aminotransferase, class V
Length = 399
Score = 48.1 bits (113), Expect = 8e-07, Method: Compositional matrix adjust.
Identities = 68/263 (25%), Positives = 108/263 (41%), Gaps = 37/263 (14%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLE 109
R +A +N E+I T T N+A G+ + H+++S EH I A L+
Sbjct: 67 RSAIASELNVPAEAIALTEDVTVGCNIAFWGID--WKPGDHLLLSDCEHPGIVATAMELQ 124
Query: 110 N-WGWEITR---LGVNHQGRVNPEDLKAA-LRHNTVLVSVIYGQSEIGTVQPIAELGKIT 164
+ EI+ + +QG +P + A LR NT L+++ + G V P+ E+ K+
Sbjct: 125 RRFNIEISTCPLMATLNQG--DPAAVIAQHLRPNTRLLAISHILWNTGQVLPLDEIVKVC 182
Query: 165 --------KTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRPG 215
K DA Q G LPL++ VD + + HK + G G G LY+
Sbjct: 183 HDSPVSTLKNGMVRVLVDAAQSVGVLPLNLINSGVDFYAFTGHKWWCGPEGLGGLYVSAE 242
Query: 216 V--ELMP--------LLGGGGQENGLRSGTQ-------ATPAIAGFGVAAELAAQ--ELE 256
+L P ++ G G + G Q A P AG A L Q +E
Sbjct: 243 ALPDLYPSFIGWRSIVMDKTGNPTGWKGGAQRYEVATSAYPLYAGLREAIALHHQWGTIE 302
Query: 257 TETGRLILLRDRLFAILADVPGL 279
+ L L+ L+++ G+
Sbjct: 303 ERYQEICRLSGYLWERLSEIDGV 325
Database: GCA_000180455.1P
Posted date: Aug 27, 2016 10:37 PM
Number of letters in database: 1,812,051
Number of sequences in database: 5822
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5822
Number of Hits to DB: 1,378,108
Number of extensions: 58726
Number of successful extensions: 187
Number of sequences better than 1.0: 8
Number of HSP's gapped: 179
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 1,812,051
Length adjustment: 87
Effective length of query: 295
Effective length of database: 1,305,537
Effective search space: 385133415
Effective search space used: 385133415
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)