BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000169095.1P
6142 sequences; 1,966,150 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
L8106_14575 Aromatic amino acid beta-eliminating lyase/threonine... 585 0.0
L8106_25555 Aromatic amino acid beta-eliminating lyase/threonine... 280 1e-90
L8106_11117 Aromatic amino acid beta-eliminating lyase/threonine... 276 4e-89
L8106_15714 Cysteine desulphurases, SufS 79 6e-17
L8106_23216 L-cysteine/cystine lyase 48 8e-07
L8106_03202 putative isopenicillin n epimerase protein 46 3e-06
L8106_00585 lysine decarboxylase 30 0.36
>L8106_14575 Aromatic amino acid beta-eliminating lyase/threonine aldolase
Length = 391
Score = 585 bits (1508), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 290/384 (75%), Positives = 330/384 (85%), Gaps = 3/384 (0%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
MQ+YLDYSATTPTRPEAIA +Q +LTQQWGN SSLHEWG RAA +LE +R QVA LINA
Sbjct: 1 MQIYLDYSATTPTRPEAIAAMQAVLTQQWGNSSSLHEWGQRAATVLERSRSQVAHLINAP 60
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120
PESIIFTSGGTEADN+AIMGVARCY+TPQH+IISS+EHSAI+EPAK+LE GW++TRL V
Sbjct: 61 PESIIFTSGGTEADNMAIMGVARCYSTPQHLIISSIEHSAIAEPAKLLEQGGWQVTRLPV 120
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
+ GRV+P DL+AA++ NTVLVSVIYGQSEIGT+QPI LGKI ++ G LFH DAVQVAG
Sbjct: 121 DRLGRVHPLDLQAAIQPNTVLVSVIYGQSEIGTLQPIEALGKIARSQGVLFHIDAVQVAG 180
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240
R+P++VQTLPVDLLSLSSHKLYG G+GALY++PGVEL PLL GGGQE LRSGTQA P
Sbjct: 181 RIPINVQTLPVDLLSLSSHKLYGPQGSGALYVKPGVELQPLLAGGGQEMKLRSGTQAIPV 240
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
IAGFG+AAELA QE+ TET RLI LRDRLF LADV LIPTGD+IHRLPHH+SF
Sbjct: 241 IAGFGMAAELAIQEMVTETPRLIQLRDRLFDGLADVTELIPTGDRIHRLPHHVSFCFLPQ 300
Query: 301 DGEK---ISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLG 357
GE ++GKTLVRQ+NLAGIGISAG+AC+SGKLSPSPILLAMGY +K ALGGIRLTLG
Sbjct: 301 TGESSSPVTGKTLVRQMNLAGIGISAGSACHSGKLSPSPILLAMGYDEKTALGGIRLTLG 360
Query: 358 KQTTAADIDWTGIVVKQILQRLIP 381
+ TT ADIDWT +V+KQILQRL P
Sbjct: 361 RDTTEADIDWTVLVLKQILQRLQP 384
>L8106_25555 Aromatic amino acid beta-eliminating lyase/threonine aldolase
Length = 395
Score = 280 bits (715), Expect = 1e-90, Method: Compositional matrix adjust.
Identities = 159/373 (42%), Positives = 228/373 (61%), Gaps = 19/373 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+YLD +ATTP +A + T+ +GNP+S+ H +G A ++ AR +A INA P
Sbjct: 6 IYLDNNATTPMDERVLAAMIPYFTEHFGNPASINHFYGWEAEAAVKKARETLASAINATP 65
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKMLENWGWEITRLGV 120
E IIFTSG TE+DNLAI GVA Y + +IS V EH+AI +P LEN G+E+T L V
Sbjct: 66 EEIIFTSGATESDNLAIKGVAEAYFSKGRHLISVVSEHNAILDPLHYLENLGFELTLLPV 125
Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180
G ++ L+ A+R +T+LVSV+ +EIG +QPIAE+GK+ + FHTDA Q G
Sbjct: 126 QSDGLIDINLLEKAIRPDTILVSVMAANNEIGVLQPIAEIGKLCRDRNIFFHTDAAQALG 185
Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLR---PGVELMPLLGGGGQENGLRSGTQA 237
++PL+V+ + +DL+SL++HK+YG G GALY+R P V+L P L GGG E G+RSGT
Sbjct: 186 KIPLNVEDMNIDLMSLTAHKIYGPKGIGALYIRRRNPRVKLAPQLHGGGHERGMRSGTLY 245
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYL 297
TP I GFG A +LA +E+++ET RL LR +L+ LA + + G L +L+ +
Sbjct: 246 TPQIVGFGEAVKLALKEMKSETKRLTNLRQQLWEKLATLGEIYLNGHPTKHLAGNLNISV 305
Query: 298 EAADGEKISGKTLVRQLNLAGI----GISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
DG+ + L G+ +S+G+AC S K+ PS +L A+G S A IR
Sbjct: 306 AGVDGQAL----------LLGLQPLMAVSSGSACTSTKIEPSHVLTALGRSDALAYASIR 355
Query: 354 LTLGKQTTAADID 366
+G+ T+ +ID
Sbjct: 356 FGIGRFNTSDEID 368
>L8106_11117 Aromatic amino acid beta-eliminating lyase/threonine aldolase
Length = 400
Score = 276 bits (705), Expect = 4e-89, Method: Compositional matrix adjust.
Identities = 159/379 (41%), Positives = 219/379 (57%), Gaps = 9/379 (2%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD +ATT I + LT +GNPSS+H +G + ++ R QVA L+ A
Sbjct: 5 IYLDNNATTGVDQAVIEAMLPYLTTYFGNPSSMHSFGGKVGQAVKQGREQVASLLGAEAS 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTS GTE DN AI +H+I + VEH A+ K LE G+ +T L V+
Sbjct: 65 EIVFTSCGTEGDNAAIRAALSAQPNKRHIITTVVEHPAVLNLCKRLEKEGYTVTYLSVDD 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G ++ +L+AAL NT LVSV+Y +E G + PI E+G+I K GALFH DAVQ G++
Sbjct: 125 KGLLDLHELEAALTGNTALVSVMYANNETGVIFPIEEIGQIVKEKGALFHVDAVQAVGKV 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PLD++T +D+L+LS HK++ G GALY+R GV P + GG QE G R GT+ I
Sbjct: 185 PLDMKTSTIDMLTLSGHKIHAPKGIGALYVRRGVRFRPFMIGGHQERGRRGGTENVAGIV 244
Query: 243 GFGVAAELAAQE-LETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
G AAELA Q LE ET + LRDRL IL+ +P + G RLP+ + +
Sbjct: 245 ALGKAAELAQQHLLEVETEK--RLRDRLEQGILSAIPNTVVNGHSTKRLPNTTNIGFKYI 302
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
+GE I + LN GI S+G+AC SG L PS +L A+G G IR +L + T
Sbjct: 303 EGEAI-----LLSLNQFGICASSGSACTSGSLEPSHVLRALGLPYSVLHGSIRFSLSRYT 357
Query: 361 TAADIDWTGIVVKQILQRL 379
T A+ID V+ Q+++RL
Sbjct: 358 TVAEIDRVLEVMPQVVERL 376
>L8106_15714 Cysteine desulphurases, SufS
Length = 420
Score = 79.3 bits (194), Expect = 6e-17, Method: Compositional matrix adjust.
Identities = 67/232 (28%), Positives = 108/232 (46%), Gaps = 12/232 (5%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHE----WGNRAALILETARMQVAGLIN 58
VYLD +A T +P+ + I + G S++H +A E AR ++ +N
Sbjct: 30 VYLD-NAATSQKPQVV--IDALRNYYEGYNSNVHRGVHTLSAKATDEYEAARQKLVKFVN 86
Query: 59 AVP-ESIIFTSGGTEADNLAIMGVA-RCYTTPQHMIISSVEHSAISEPAKML-ENWGWEI 115
A + II+T +EA NL +I+S +EH + P +ML + G +
Sbjct: 87 AASYQEIIYTRNASEAINLVAYSWGLNNIKAGDEIILSVMEHHSNLVPWQMLAQRTGAVL 146
Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175
+ + + E K+ + T LVSV++ + +G + P+ E+ KI +GA DA
Sbjct: 147 KFVELTETQTFDLEQFKSLISPQTKLVSVVHVSNVLGCINPVEEICKIAHKNGAKVLIDA 206
Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225
Q +P+DVQ + D L S HK+ G G LY + + + P LGGG
Sbjct: 207 CQSLPHMPIDVQAMDCDWLVGSGHKMCAPTGIGFLYGKLEILKAMPPFLGGG 258
>L8106_23216 L-cysteine/cystine lyase
Length = 397
Score = 48.1 bits (113), Expect = 8e-07, Method: Compositional matrix adjust.
Identities = 47/178 (26%), Positives = 81/178 (45%), Gaps = 13/178 (7%)
Query: 47 ETARMQ--VAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEP 104
E+A+M+ +A + E+I T T N+A+ G+ + H+++S EH I
Sbjct: 62 ESAQMRDCMARELGVSAETITLTENVTVGCNIALWGID--WQPGDHLLLSDCEHPGIIAI 119
Query: 105 AKMLENWGWEITRLGVNHQGRVNPED----LKAALRHNTVLVSVIYGQSEIGTVQPIAEL 160
+ L+ +++ + +N D ++ L+ NT L+ + + G V P+ E+
Sbjct: 120 IQELQR-RFQVEVSVCPLRETLNQGDPVSVIETHLQPNTRLLVISHILWNTGQVLPLGEI 178
Query: 161 GKITKTH---GALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG-LGAGALYLRP 214
K H DA Q G LPL++ L VD + + HK + G G+G LY P
Sbjct: 179 VKCCHNHPQGKVRVLVDAAQSVGVLPLNLTALGVDFYAFTGHKWWCGPEGSGGLYTSP 236
>L8106_03202 putative isopenicillin n epimerase protein
Length = 399
Score = 46.2 bits (108), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 42/185 (22%), Positives = 79/185 (42%), Gaps = 11/185 (5%)
Query: 35 LHEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIIS 94
+ EW + +L+ AR Q+A + + + ++F T N + + C++ ++ +
Sbjct: 60 VREWES----LLDEARRQLAEFLGVIADELVFVPNATTGINSVLRSL--CFSPTDELLTT 113
Query: 95 SVEHSAISEPAKML-ENWGWEITRLGVNHQGRVNPEDLKAALRH---NTVLVSVIYGQSE 150
+ E++A + E WG ++T + E + + L T LV + + S+
Sbjct: 114 NHEYNACRNALNFVAERWGAKVTIANIPFPLNSPDEIISSILDQITPRTKLVLIDHISSQ 173
Query: 151 IGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGA 209
G + P+ L K + G D G LPL++ + S + HK L GA
Sbjct: 174 TGLIFPLQPLIKELNSRGIESLVDGAHAPGMLPLNLPEIGATYYSGNCHKWLSAPKGAAF 233
Query: 210 LYLRP 214
LY+ P
Sbjct: 234 LYVHP 238
>L8106_00585 lysine decarboxylase
Length = 516
Score = 30.4 bits (67), Expect = 0.36, Method: Compositional matrix adjust.
Identities = 26/112 (23%), Positives = 50/112 (44%), Gaps = 23/112 (20%)
Query: 110 NWGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIY----GQSEIGTVQPIAELGK--- 162
N W++T + PE ++ AL+H+ + +V+ Q G ++ IA++
Sbjct: 166 NAEWDLTY-------SITPEAVETALKHHADIKAVLMVYPTYQGICGNIEQIAKITHDYN 218
Query: 163 ----ITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGAL 210
+ + HG F+ A LP+ T DL+ S+HK+ G + ++
Sbjct: 219 IPLLVDEAHGPHFYFHA-----DLPIAALTAGADLVVQSTHKVLGAMTQASM 265
Database: GCA_000169095.1P
Posted date: Aug 27, 2016 10:40 PM
Number of letters in database: 1,966,150
Number of sequences in database: 6142
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 6142
Number of Hits to DB: 1,485,810
Number of extensions: 62383
Number of successful extensions: 167
Number of sequences better than 1.0: 8
Number of HSP's gapped: 160
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 1,966,150
Length adjustment: 88
Effective length of query: 294
Effective length of database: 1,425,654
Effective search space: 419142276
Effective search space used: 419142276
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)