BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000155635.1P
2771 sequences; 822,309 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CPCC7001_1625 nifS cysteine desulfurase 256 5e-82
CPCC7001_1906 iscS cysteine desulfurase involved in Fe-S cluster... 213 1e-65
CPCC7001_1295 cysteine desulfurase 2 99 3e-24
CPCC7001_905 serine:pyruvate/alanine:glyoxylate aminotransferase 45 4e-06
CPCC7001_625 cefD isopenicillin N epimerase 39 3e-04
>CPCC7001_1625 nifS cysteine desulfurase
Length = 402
Score = 256 bits (655), Expect = 5e-82, Method: Compositional matrix adjust.
Identities = 166/391 (42%), Positives = 226/391 (57%), Gaps = 18/391 (4%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD AT P E + + ++ + W NPSSLH +G +AA LE AR+Q AG + P+
Sbjct: 8 LYLDACATAPPAEEVLNAMASVHRRAWANPSSLHGFGLQAAEALERARLQAAGCLGCDPD 67
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
++FTSGGTEA + A++G+A + T ++I++VEH A A L GW++ + V+
Sbjct: 68 CLVFTSGGTEAIHTALLGMAAAHPT-GRLVITAVEHPATVAAAAALRQRGWQVAVVPVDR 126
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
G V+ + +A L T L SVI+GQSE+GTVQP+ +G++ + G H DAVQV G
Sbjct: 127 AGLVDLDRFEALLAPPTRLASVIWGQSEVGTVQPVQSIGELCRRAGVRLHVDAVQVVGHQ 186
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
PL LPVDLLS ++HKL G G GAL +RPGV PLL GGGQE+G R GT+ A
Sbjct: 187 PLAFNRLPVDLLSCAAHKLQGPRGVGALLVRPGVPFEPLLPGGGQESGRRGGTEPVALAA 246
Query: 243 GFGVAAELAAQELETETGR--LILLRDRLFAILADVPGLI-----PTGDKIHRLPHHLSF 295
GF +LA + L+ + G L LRD L L PGL P GD RLPHH+S
Sbjct: 247 GFAAGLQLATERLQRQGGEDPLRPLRDALLHQLLQHPGLRLSGVDPFGDPAQRLPHHISL 306
Query: 296 YLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLS------PSPILLAMGYSQKAAL 349
+ G +SG+ LV+ L G+ S+G+AC+SG + PSP+L AMGY A
Sbjct: 307 LVNDRRGRPVSGRRLVQALWRHGVAASSGSACSSGAGASAVAGMPSPVLSAMGYPPAEAA 366
Query: 350 GGIRLTLGKQTTAADIDWTGIVVKQILQRLI 380
G+RL+LG AD+D V Q L R +
Sbjct: 367 SGVRLSLGPWLEPADLD----AVPQRLTRAL 393
>CPCC7001_1906 iscS cysteine desulfurase involved in Fe-S cluster formation
Length = 379
Score = 213 bits (542), Expect = 1e-65, Method: Compositional matrix adjust.
Identities = 144/385 (37%), Positives = 211/385 (54%), Gaps = 14/385 (3%)
Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINA 59
M YLD+ A+TP P A ++ + NPSS LH AA +E AR QVA ++A
Sbjct: 1 MLAYLDHHASTPCDPAVQAAMEPWWSVHAANPSSRLHRPALEAAAAVERARGQVAQALDA 60
Query: 60 VPES-IIFTSGGTEADNLAIMGVARCY----TTPQHMIISSVEHSAISEPAKMLENWGWE 114
+ ++FTSG TEA+NLA+ GV Q ++ + EH A+ +P + LE G+
Sbjct: 61 AEATAVVFTSGATEANNLALKGVLEAALERGEPRQRLLTLATEHRAVLDPLRYLERHGFP 120
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+T L V G V+ E AAL +L +V+ +EIG +QP+A + ++ + G L H D
Sbjct: 121 LTVLPVQPDGLVDLEAFAAALGPEVLLAAVMAANNEIGVLQPLAAIAELCRARGVLLHVD 180
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSG 234
A Q AG +PL + L +DLLS+S HK YG G GAL +R GV + P + GGGQE GLR+G
Sbjct: 181 AAQAAGHIPLAMADLGIDLLSVSGHKCYGPKGVGALLVRSGVAVAPQIHGGGQEGGLRAG 240
Query: 235 TQATPAIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLS 294
T A P + G G A + + +GRL LRDRL+ L + G+ G RL H+L+
Sbjct: 241 TVAVPLVVGLGEALQRCCADQPERSGRLGALRDRLWQGLEPLGGMRCNGVGAPRLSHNLN 300
Query: 295 FYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRL 354
+ DG T + +L + +S+G+AC+ G SPS +L A+G S++ A +R
Sbjct: 301 ITVGGVDG------TALHRLLRRDLAVSSGSACSQG--SPSHVLAALGLSRQEAAASVRF 352
Query: 355 TLGKQTTAADIDWTGIVVKQILQRL 379
LG+ T A+I V+ + +L
Sbjct: 353 GLGRSTGEAEITRAIDAVRAAVDQL 377
>CPCC7001_1295 cysteine desulfurase 2
Length = 436
Score = 99.4 bits (246), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 117/389 (30%), Positives = 171/389 (43%), Gaps = 42/389 (10%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAV- 60
+YLD++AT+ + + +Q N H+ RA E AR + A I A
Sbjct: 48 IYLDHAATSQKPRQVLEALQRYYDHDNANVHRGAHQLSARATEGFEGARAKAAAFIGAAS 107
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKMLE-NWGWE 114
P I+FT +EA NL VAR + +++S +EH + P + L G
Sbjct: 108 PREIVFTRNASEAINL----VARTWGEANLREGDEVVLSVMEHHSNLVPWQQLAARTGCV 163
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G+ G ++ +DL+A L T LVS+++ + +G + PIAE+ ++ GAL D
Sbjct: 164 LRHVGLTDSGELDMDDLRAKLNPRTRLVSLVHVSNTLGCLNPIAEISELAHAVGALVLVD 223
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGG---QENG 230
A Q +P++V L D L+ SSHKL G G G L+ R +E MP GGG Q+
Sbjct: 224 ACQSLPHMPVNVAELGCDFLAGSSHKLCGPTGMGFLWGREALLEAMPPFLGGGEMIQDVY 283
Query: 231 LRSGTQA---------TPAIA---GFGVAAE-LAAQELETETGRLILLRDRLFAILADVP 277
L T A TPAI G G A + L A L+ L RLF L+ +
Sbjct: 284 LDHSTWADLPHKFEAGTPAIGEAIGMGAALDYLQAIGLDRIHSWEQQLTCRLFERLSAID 343
Query: 278 GLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPI 337
G+ G + PH + DG + L L+ AGI I +G C P+
Sbjct: 344 GVRILGPTPEQQPHRGALAAFTVDG--LHANDLAALLDSAGICIRSGHHCT------QPL 395
Query: 338 LLAMGYSQKAALGGIRLTLGKQTTAADID 366
G G R +L TT +ID
Sbjct: 396 HRLYGLP-----GSARASLSFTTTPEEID 419
>CPCC7001_905 serine:pyruvate/alanine:glyoxylate aminotransferase
Length = 397
Score = 44.7 bits (104), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 26/91 (28%), Positives = 48/91 (52%), Gaps = 2/91 (2%)
Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+++AA++ H ++++++ ++ G QP+ +G++ +THG L D V G +P+ +
Sbjct: 137 EEIEAAVKQHRPTVLALVHAETSTGIRQPMEGIGEVCRTHGCLLLLDTVTSLGAVPVHLD 196
Query: 188 TLPVDL-LSLSSHKLYGGLGAGALYLRPGVE 217
VDL S S L G G + P E
Sbjct: 197 AWQVDLAYSCSQKGLSCPPGLGPFTMGPRAE 227
>CPCC7001_625 cefD isopenicillin N epimerase
Length = 387
Score = 38.9 bits (89), Expect = 3e-04, Method: Compositional matrix adjust.
Identities = 52/208 (25%), Positives = 74/208 (35%), Gaps = 21/208 (10%)
Query: 50 RMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML- 108
R ++ G + P + FT T L + G+ +T ++I EH + + L
Sbjct: 60 RRRLGGWLGVEPHRLAFTENVTSGCVLPLWGLP--WTPGDELLIGDCEHPGVVAACRELG 117
Query: 109 -------ENWGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELG 161
W R L+AAL T LV + + G + PIA
Sbjct: 118 HRQGLVVRTWAVGDLRGDAASTDAAVLNRLEAALTPRTRLVVLSHLLWNTGQIMPIAGAA 177
Query: 162 KITKTHGA--LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYG---GLGAGALYLRPGV 216
+ K H A DA Q G +P D+ + + HK GLG AL R
Sbjct: 178 ETLKGHPAQPWLLVDAAQALGCIPAQAAAQAADIYACTGHKWCCGPEGLGVVALSERLLA 237
Query: 217 ELMPLLGGGGQENGLRSGTQATPAIAGF 244
E P L G RS + A +GF
Sbjct: 238 ESRPTL------IGWRSLSHEATADSGF 259
Database: GCA_000155635.1P
Posted date: Aug 27, 2016 11:15 PM
Number of letters in database: 822,309
Number of sequences in database: 2771
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2771
Number of Hits to DB: 708,156
Number of extensions: 33973
Number of successful extensions: 128
Number of sequences better than 1.0: 8
Number of HSP's gapped: 120
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 822,309
Length adjustment: 82
Effective length of query: 300
Effective length of database: 595,087
Effective search space: 178526100
Effective search space used: 178526100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)