BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000153065.1P
2770 sequences; 795,631 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RS9917_06935 NifS-like aminotransferase class-V 240 8e-76
RS9917_12650 possible cysteine desulfurase (class-V aminotransfe... 204 5e-62
RS9917_07755 cysteine desulfurases, SufS subfamily protein 94 2e-22
RS9917_11800 serine:pyruvate/alanine:glyoxylate aminotransferase 42 3e-05
RS9917_11975 Possible glutamyl-tRNA reductase 29 0.40
>RS9917_06935 NifS-like aminotransferase class-V
Length = 390
Score = 240 bits (612), Expect = 8e-76, Method: Compositional matrix adjust.
Identities = 150/383 (39%), Positives = 214/383 (55%), Gaps = 14/383 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+ LDY ATTP E I + + WGNPSS H G AA + AR ++ + P
Sbjct: 11 ICLDYQATTPCAAEVIEAMAPWWREHWGNPSSRQHRLGLMAAAAVSEARERLGRCLGVTP 70
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTT----PQHMIISSVEHSAISEPAKMLENWGWEITR 117
E I+FTSG TEA+NLA++G AR P H+I + EH A+ +P + LE G+++T
Sbjct: 71 ERIVFTSGATEANNLALLGHARALARQRGRPGHLISVATEHHAVLDPLRQLEREGFQLTL 130
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
L G + P L+AALR +T LVSV+ +EIG +QP+A LG++ + G H+D Q
Sbjct: 131 LTPAEDGLLQPAQLEAALRDDTQLVSVMAANNEIGVLQPLASLGRLCRERGITLHSDGAQ 190
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQA 237
G + + L +DL SLS+HK+YG G GAL + + PL GGGQE GLR GT
Sbjct: 191 AFGAMAFEPDALGIDLTSLSAHKIYGPKGIGALVRPLALPIEPLQWGGGQEGGLRPGTLP 250
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFY 296
P I GF AAELA ++ + T RL RDRL+ + +P L+ G RL H+L+
Sbjct: 251 VPLIVGFATAAELALRDQDRRTQRLRQWRDRLWRGLRTALPDLLCNGTLEQRLAHNLNIT 310
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
+ +SG L +QL + S+G+AC+ G+ PS +L A+G S++ A +RL+L
Sbjct: 311 IPG-----VSGSRLHQQLR-RHVLCSSGSACSRGE--PSHVLRALGRSRQEAEASLRLSL 362
Query: 357 GKQTTAADIDWTGIVVKQILQRL 379
G TT +ID + + + RL
Sbjct: 363 GWATTEHEIDAAIAAIAETVTRL 385
>RS9917_12650 possible cysteine desulfurase (class-V aminotransferase family
protein)
Length = 407
Score = 204 bits (519), Expect = 5e-62, Method: Compositional matrix adjust.
Identities = 144/389 (37%), Positives = 210/389 (53%), Gaps = 13/389 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD AT P R + + + + WGN SSLH G RAA LE +R+++A A +
Sbjct: 13 LYLDACATAPPRADVLQHMVAVQRSAWGNASSLHPQGLRAAEQLERSRLELAQAFEADLD 72
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
+I TSG TE+ + A++GVARC P ++IS+VEH A+ A + GW+I R V+
Sbjct: 73 EVIVTSGATESIHFALLGVARCQP-PGRLVISAVEHPAVEAAASQMVERGWQIARWPVDG 131
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
+G + + L+A L T LVS+I+GQSE+GT+QP+ +G+ + G H DA QV +
Sbjct: 132 EGTIRLDQLEALLAPPTRLVSLIWGQSEVGTLQPVQRVGEACRARGISLHVDATQVVSQG 191
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELM--PLLGGGGQENGLRSGTQATPA 240
+ LPVDLLS S+HK G G G L R + P+ GGGQE GLR GTQ
Sbjct: 192 RPAWRRLPVDLLSASAHKCGGPKGVGLLLRRRTLRERHGPIQAGGGQEGGLRGGTQPVVL 251
Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPG----------LIPTGDKIHRLP 290
+AG A L L+ + + R+ A+ L G + RLP
Sbjct: 252 MAGMARAISLLEPWLDPQAPGVDPGLSRMGAVQQRRDRLLQQLLDLAPLQRLGAPVQRLP 311
Query: 291 HHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALG 350
HH++ G + G+++VR L G+ IS+G+AC SG+ SP+L AMG +
Sbjct: 312 HHIALLAADPQGRPLPGRSVVRALAAEGVAISSGSACASGRDGGSPVLAAMGIAPDWCRS 371
Query: 351 GIRLTLGKQTTAADIDWTGIVVKQILQRL 379
G+RL+LG +AAD+D +V+ L+R+
Sbjct: 372 GLRLSLGPWLSAADLDRVAALVQGALERV 400
>RS9917_07755 cysteine desulfurases, SufS subfamily protein
Length = 433
Score = 94.4 bits (233), Expect = 2e-22, Method: Compositional matrix adjust.
Identities = 112/389 (28%), Positives = 165/389 (42%), Gaps = 42/389 (10%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61
+YLD++AT+ + +Q + N H+ RA E AR A I A
Sbjct: 45 IYLDHAATSQKPRVVLETLQHFYSADNANVHRGAHQLSARATESFEAARTITARFIAAAS 104
Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKML-ENWGWE 114
I+FT +EA NL VAR + ++++ +EH + P ++L + G
Sbjct: 105 SHEIVFTRNASEAINL----VARSWGDANLKEGDEVLLTVMEHHSNLVPWQLLAQRTGCV 160
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G+ GR++ EDL+ L T LVS+++ + +G P+ E+ + + GA D
Sbjct: 161 LRHVGLTESGRLDLEDLRTKLSARTRLVSLVHISNTLGCCNPVPEVVSLARQQGAKVLLD 220
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGG---QENG 230
A Q PL+V L VD L SSHKL G G G L+ +E MP GGG Q+
Sbjct: 221 ACQSLAHQPLNVGALGVDFLVGSSHKLCGPTGMGFLWASEALLEAMPPFLGGGEMIQDVF 280
Query: 231 LRSGTQA---------TPAIA---GFGVA-AELAAQELETETGRLILLRDRLFAILADVP 277
L T A TPAI G G A L + LET L LF L +
Sbjct: 281 LDHSTWAELPHKFEAGTPAIGEAIGMGAAITYLQSLGLETIQAWEAQLTRHLFRRLEAID 340
Query: 278 GLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPI 337
G+ G + P + DG + + L+ +GI I +G C P+
Sbjct: 341 GVRILGPTPEQQPGRGALATFLVDG--VHANDIAALLDCSGICIRSGHHCC------QPL 392
Query: 338 LLAMGYSQKAALGGIRLTLGKQTTAADID 366
G + A R +L TT A+ID
Sbjct: 393 HRLYGVTASA-----RASLSFTTTLAEID 416
>RS9917_11800 serine:pyruvate/alanine:glyoxylate aminotransferase
Length = 397
Score = 41.6 bits (96), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 27/91 (29%), Positives = 44/91 (48%), Gaps = 2/91 (2%)
Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+L+AAL H ++++++ ++ G QP+ +G + + H L D V G +PL +
Sbjct: 143 EELEAALIEHRPAILAMVHAETSTGICQPMEGVGDLCRKHDCLLLLDTVTSLGGVPLFLD 202
Query: 188 TLPVDL-LSLSSHKLYGGLGAGALYLRPGVE 217
VDL S S L G G + P E
Sbjct: 203 AWKVDLAYSCSQKGLSCPPGLGPFTMGPRAE 233
>RS9917_11975 Possible glutamyl-tRNA reductase
Length = 429
Score = 28.9 bits (63), Expect = 0.40, Method: Compositional matrix adjust.
Identities = 57/219 (26%), Positives = 88/219 (40%), Gaps = 37/219 (16%)
Query: 135 LRHNTVLVSVIYGQSEI-GTVQPIAELGKITKTHGALFH---TDAVQVAGRLPLDVQTLP 190
LR + L S++ G+ +I V+ + LG+ K+ G + + T AV R+
Sbjct: 101 LRVSAGLDSLVLGEGQILSQVKKMMRLGQEHKSIGPILNRLLTQAVSTGKRV-------- 152
Query: 191 VDLLSLSSHKLYGGLGAGALYLRPG-VELMPL-LGGGGQENGLRSGTQATPAIAGFGVAA 248
+ LG GA+ + VEL L LG + L + A+ G G +
Sbjct: 153 ---------RTETNLGTGAVSISSAAVELAQLKLGQARGLDQLVTLESEQVAVVGAGRMS 203
Query: 249 ELAAQELETE--TGRLIL--LRDRLFAILADVPGLIPTGDKIHRLPHHLS-----FYLEA 299
L Q L+ + +G ++L R+R + AD PGL + L H LS F A
Sbjct: 204 RLLLQHLQAKGCSGVVLLNRTRERAQMLAADFPGLPAQCRTLEELDHCLSTCSLVFTSTA 263
Query: 300 ADGEKISGKTLVR-----QLNLAGIGISAGAACNSGKLS 333
AD I L R L L IG+ A + ++
Sbjct: 264 ADDPIIDAARLQRLNRRSSLRLIDIGVPRNIASDVADIA 302
Database: GCA_000153065.1P
Posted date: Aug 27, 2016 11:06 PM
Number of letters in database: 795,631
Number of sequences in database: 2770
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2770
Number of Hits to DB: 670,573
Number of extensions: 31672
Number of successful extensions: 133
Number of sequences better than 1.0: 8
Number of HSP's gapped: 126
Number of HSP's successfully gapped: 8
Length of query: 382
Length of database: 795,631
Length adjustment: 82
Effective length of query: 300
Effective length of database: 568,491
Effective search space: 170547300
Effective search space used: 170547300
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)