BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000153045.1P
3346 sequences; 894,594 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
WH5701_13095 possible cysteine desulfurase (class-V aminotransfe... 262 3e-84
WH5701_05440 cysteine desulfurase NifS 199 3e-60
WH5701_15831 putative cysteine desulfurase or selenocysteine lyase 103 9e-26
WH5701_13585 serine:pyruvate/alanine:glyoxylate aminotransferase 38 7e-04
WH5701_13595 Pyridoxal-dependent decarboxylase family protein 33 0.013
>WH5701_13095 possible cysteine desulfurase (class-V aminotransferase family
protein)
Length = 397
Score = 262 bits (669), Expect = 3e-84, Method: Compositional matrix adjust.
Identities = 157/366 (42%), Positives = 216/366 (59%), Gaps = 3/366 (0%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD SA P + + W NPSSLH +G AA LE +R +A L+ A +
Sbjct: 14 LYLDASAGAPPAEAVLQAMAAATRTAWANPSSLHGYGLAAADSLERSRQAIAALLGAEAQ 73
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
+ F SGGTEA +LA++ A P ++ISSVEH A+ A+ L GW + RL V+H
Sbjct: 74 QLTFCSGGTEAAHLALL-GAAAALPPGRLLISSVEHPAVEAAAERLIGSGWTLERLPVDH 132
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
GR+ + L+A L T LVS+I+GQSE+GT+QP+ + + + G + H+DAVQV G
Sbjct: 133 LGRLRLDRLEALLEPPTRLVSLIWGQSEVGTLQPLQAVAERCRRAGVILHSDAVQVVGHR 192
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242
P+D ++ VDLLS ++HKL G G GAL RPG+EL P++ GGGQE GLRSGT+ +A
Sbjct: 193 PIDFASMQVDLLSFTAHKLQGPRGIGALLARPGLELRPMIEGGGQEGGLRSGTEPVVLVA 252
Query: 243 GFGVAAELAAQELETETGR--LILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300
GF A LA + LE G+ L RD L L + G+ TG RLPHHLS + A
Sbjct: 253 GFAEALRLAQRRLELNAGQDPLEPWRDGLLQSLISLGGVELTGCPSQRLPHHLSLLVRDA 312
Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360
G + G+ LV+ L G+ +S+G+AC G+ SP+L AMGYS A G+RL+LG
Sbjct: 313 GGRPLPGRRLVQALWRQGLAVSSGSACQRGRAGGSPVLQAMGYSPDLAASGLRLSLGPWL 372
Query: 361 TAADID 366
+ +D
Sbjct: 373 SQEQLD 378
>WH5701_05440 cysteine desulfurase NifS
Length = 376
Score = 199 bits (506), Expect = 3e-60, Method: Compositional matrix adjust.
Identities = 146/378 (38%), Positives = 207/378 (54%), Gaps = 10/378 (2%)
Query: 4 YLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVPE 62
YLD+ ATTP P+ +A + ++ + NPSS H G AA ++ AR +A + PE
Sbjct: 5 YLDHQATTPCDPDVVAAMAPYWSELFANPSSRSHRLGLEAAAAVDLARSSLARPLGVAPE 64
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
++FTSG TEA+NLAI G+ +H+I + EH A+ EP + L G+ +T L V
Sbjct: 65 QVVFTSGATEANNLAIKGLVEARRQRGRHLITLATEHRAVLEPLRYLAGHGYSLTVLPVQ 124
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
G V+ ALR +T+LVSV+ +EIG +QP+A +G + + G H+D Q+ G
Sbjct: 125 RDGLVDLNRFSEALRDDTLLVSVMAANNEIGVLQPLAAIGTLCRERGIALHSDGAQIFGN 184
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAI 241
P L +DL SLS HK+YG G GAL L G+ L P GGGQE GLR GT P I
Sbjct: 185 RPFSPGELGLDLFSLSGHKIYGPKGIGALVLAEGMILAPQQHGGGQEGGLRGGTLPVPLI 244
Query: 242 AGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301
G AAELA+ + + RL LRDRL A L ++ G+ G HRL H+L+ +LE D
Sbjct: 245 VGLQRAAELASHDWKERQQRLGGLRDRLLAGLLELGGVRVNGSLEHRLAHNLNVHLEGVD 304
Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361
G ++ + L RQL ++G + + + +L A+G + A IR LG+ TT
Sbjct: 305 GTQLH-RALRRQLAVSGGSACSSGSPSH-------VLAALGLTPAEAAASIRFGLGRSTT 356
Query: 362 AADIDWTGIVVKQILQRL 379
++ID V LQ+L
Sbjct: 357 TSEIDQAVEAVAGALQKL 374
>WH5701_15831 putative cysteine desulfurase or selenocysteine lyase
Length = 443
Score = 103 bits (258), Expect = 9e-26, Method: Compositional matrix adjust.
Identities = 111/388 (28%), Positives = 158/388 (40%), Gaps = 40/388 (10%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINA-V 60
+YLD++AT+ + +Q N H+ RA E AR + A + A
Sbjct: 55 IYLDHAATSQKPRAVLDALQRYYRHDNANVHRGAHQLSARATDAFEGARDKTARFVGAAT 114
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV--EHSAISEPAKMLENWGWEITRL 118
P I+FT TEA NL + P I+ +V HS + ++ E G +
Sbjct: 115 PREIVFTRNATEAINLVARSWGDAFLRPGDEIVLTVMEHHSNLVPWQQLAERTGARLRHA 174
Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178
G+ G ++ EDL+ + T LVSV+ + +G PI + ++T GAL DA Q
Sbjct: 175 GLTASGELDLEDLRDQISERTRLVSVVQISNTLGCHNPIGAIAELTHAAGALLLVDACQS 234
Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ---------- 227
LP++VQ L D L SSHKL G G G L+ R +E MP GGG+
Sbjct: 235 LPHLPVNVQALGADFLVGSSHKLCGPTGMGFLWAREALLEAMPPFLGGGEMIQDVYLDHS 294
Query: 228 -----ENGLRSGTQATPAIAGFGVAAE-LAAQELETETGRLILLRDRLFAILADVPGLIP 281
+ +GT A G G A + L A L+ L LF L + GL
Sbjct: 295 TWAELPHKFEAGTPAIGEAVGMGAAIDYLNALGLDRIHAWEQRLTAHLFERLQAIEGLRV 354
Query: 282 TGDKIHRLPHH---LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPIL 338
G + P +F +E IS L+ +GI I +G C P+
Sbjct: 355 LGPTPQQQPERGALAAFVVEGLHANDISAL-----LDASGICIRSGHHCT------QPLH 403
Query: 339 LAMGYSQKAALGGIRLTLGKQTTAADID 366
G S G R +L TT +ID
Sbjct: 404 RHYGIS-----GSARASLSFTTTIEEID 426
>WH5701_13585 serine:pyruvate/alanine:glyoxylate aminotransferase
Length = 402
Score = 37.7 bits (86), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
E+++ AL H ++++++ ++ G QP+ +G++ + + L D V G +PL +
Sbjct: 146 EEIEQALASHRPAILAMVHAETSTGVCQPMEGIGELCRRYDCLLLLDTVTSLGAVPLHLD 205
Query: 188 TLPVDLLSLSSHK 200
VDL S K
Sbjct: 206 AWGVDLAYSCSQK 218
>WH5701_13595 Pyridoxal-dependent decarboxylase family protein
Length = 484
Score = 33.5 bits (75), Expect = 0.013, Method: Compositional matrix adjust.
Identities = 29/122 (23%), Positives = 57/122 (46%), Gaps = 12/122 (9%)
Query: 65 IFTSGGTEADNLAIMGVARCYT---TPQHMIISSVE-HSAISEPAKMLENWGWEITRLGV 120
+ SGG+ ++ +A++ P+ +++ ++ H+++ + A ++ + RL +
Sbjct: 157 VPASGGSLSNLMALVAARESRGLRGVPEAVVLCGLDSHTSLEKAAMVMGLPAAALHRLPL 216
Query: 121 NHQGRVNP-------EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173
+ GR+ P E L AA LV+ G + G V P+ EL + + HG H
Sbjct: 217 DGDGRLEPALVAEYLESLSAAGVPVIALVAT-AGTTVRGAVDPLVELASLCRRHGVWLHI 275
Query: 174 DA 175
DA
Sbjct: 276 DA 277
Database: GCA_000153045.1P
Posted date: Aug 27, 2016 11:08 PM
Number of letters in database: 894,594
Number of sequences in database: 3346
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3346
Number of Hits to DB: 758,261
Number of extensions: 35337
Number of successful extensions: 131
Number of sequences better than 1.0: 6
Number of HSP's gapped: 124
Number of HSP's successfully gapped: 6
Length of query: 382
Length of database: 894,594
Length adjustment: 82
Effective length of query: 300
Effective length of database: 620,222
Effective search space: 186066600
Effective search space used: 186066600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)