BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000153045.1P 3346 sequences; 894,594 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value WH5701_13095 possible cysteine desulfurase (class-V aminotransfe... 262 3e-84 WH5701_05440 cysteine desulfurase NifS 199 3e-60 WH5701_15831 putative cysteine desulfurase or selenocysteine lyase 103 9e-26 WH5701_13585 serine:pyruvate/alanine:glyoxylate aminotransferase 38 7e-04 WH5701_13595 Pyridoxal-dependent decarboxylase family protein 33 0.013 C5ddf0aa94e021c824acdf77d98afdef >WH5701_13095 possible cysteine desulfurase (class-V aminotransferase family protein) Length = 397 Score = 262 bits (669), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 157/366 (42%), Positives = 216/366 (59%), Gaps = 3/366 (0%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +YLD SA P + + W NPSSLH +G AA LE +R +A L+ A + Sbjct: 14 LYLDASAGAPPAEAVLQAMAAATRTAWANPSSLHGYGLAAADSLERSRQAIAALLGAEAQ 73 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122 + F SGGTEA +LA++ A P ++ISSVEH A+ A+ L GW + RL V+H Sbjct: 74 QLTFCSGGTEAAHLALL-GAAAALPPGRLLISSVEHPAVEAAAERLIGSGWTLERLPVDH 132 Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182 GR+ + L+A L T LVS+I+GQSE+GT+QP+ + + + G + H+DAVQV G Sbjct: 133 LGRLRLDRLEALLEPPTRLVSLIWGQSEVGTLQPLQAVAERCRRAGVILHSDAVQVVGHR 192 Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242 P+D ++ VDLLS ++HKL G G GAL RPG+EL P++ GGGQE GLRSGT+ +A Sbjct: 193 PIDFASMQVDLLSFTAHKLQGPRGIGALLARPGLELRPMIEGGGQEGGLRSGTEPVVLVA 252 Query: 243 GFGVAAELAAQELETETGR--LILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300 GF A LA + LE G+ L RD L L + G+ TG RLPHHLS + A Sbjct: 253 GFAEALRLAQRRLELNAGQDPLEPWRDGLLQSLISLGGVELTGCPSQRLPHHLSLLVRDA 312 Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360 G + G+ LV+ L G+ +S+G+AC G+ SP+L AMGYS A G+RL+LG Sbjct: 313 GGRPLPGRRLVQALWRQGLAVSSGSACQRGRAGGSPVLQAMGYSPDLAASGLRLSLGPWL 372 Query: 361 TAADID 366 + +D Sbjct: 373 SQEQLD 378 >WH5701_05440 cysteine desulfurase NifS Length = 376 Score = 199 bits (506), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 146/378 (38%), Positives = 207/378 (54%), Gaps = 10/378 (2%) Query: 4 YLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVPE 62 YLD+ ATTP P+ +A + ++ + NPSS H G AA ++ AR +A + PE Sbjct: 5 YLDHQATTPCDPDVVAAMAPYWSELFANPSSRSHRLGLEAAAAVDLARSSLARPLGVAPE 64 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121 ++FTSG TEA+NLAI G+ +H+I + EH A+ EP + L G+ +T L V Sbjct: 65 QVVFTSGATEANNLAIKGLVEARRQRGRHLITLATEHRAVLEPLRYLAGHGYSLTVLPVQ 124 Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181 G V+ ALR +T+LVSV+ +EIG +QP+A +G + + G H+D Q+ G Sbjct: 125 RDGLVDLNRFSEALRDDTLLVSVMAANNEIGVLQPLAAIGTLCRERGIALHSDGAQIFGN 184 Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAI 241 P L +DL SLS HK+YG G GAL L G+ L P GGGQE GLR GT P I Sbjct: 185 RPFSPGELGLDLFSLSGHKIYGPKGIGALVLAEGMILAPQQHGGGQEGGLRGGTLPVPLI 244 Query: 242 AGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAAD 301 G AAELA+ + + RL LRDRL A L ++ G+ G HRL H+L+ +LE D Sbjct: 245 VGLQRAAELASHDWKERQQRLGGLRDRLLAGLLELGGVRVNGSLEHRLAHNLNVHLEGVD 304 Query: 302 GEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTT 361 G ++ + L RQL ++G + + + +L A+G + A IR LG+ TT Sbjct: 305 GTQLH-RALRRQLAVSGGSACSSGSPSH-------VLAALGLTPAEAAASIRFGLGRSTT 356 Query: 362 AADIDWTGIVVKQILQRL 379 ++ID V LQ+L Sbjct: 357 TSEIDQAVEAVAGALQKL 374 >WH5701_15831 putative cysteine desulfurase or selenocysteine lyase Length = 443 Score = 103 bits (258), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 111/388 (28%), Positives = 158/388 (40%), Gaps = 40/388 (10%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINA-V 60 +YLD++AT+ + +Q N H+ RA E AR + A + A Sbjct: 55 IYLDHAATSQKPRAVLDALQRYYRHDNANVHRGAHQLSARATDAFEGARDKTARFVGAAT 114 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV--EHSAISEPAKMLENWGWEITRL 118 P I+FT TEA NL + P I+ +V HS + ++ E G + Sbjct: 115 PREIVFTRNATEAINLVARSWGDAFLRPGDEIVLTVMEHHSNLVPWQQLAERTGARLRHA 174 Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178 G+ G ++ EDL+ + T LVSV+ + +G PI + ++T GAL DA Q Sbjct: 175 GLTASGELDLEDLRDQISERTRLVSVVQISNTLGCHNPIGAIAELTHAAGALLLVDACQS 234 Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGGQ---------- 227 LP++VQ L D L SSHKL G G G L+ R +E MP GGG+ Sbjct: 235 LPHLPVNVQALGADFLVGSSHKLCGPTGMGFLWAREALLEAMPPFLGGGEMIQDVYLDHS 294 Query: 228 -----ENGLRSGTQATPAIAGFGVAAE-LAAQELETETGRLILLRDRLFAILADVPGLIP 281 + +GT A G G A + L A L+ L LF L + GL Sbjct: 295 TWAELPHKFEAGTPAIGEAVGMGAAIDYLNALGLDRIHAWEQRLTAHLFERLQAIEGLRV 354 Query: 282 TGDKIHRLPHH---LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPIL 338 G + P +F +E IS L+ +GI I +G C P+ Sbjct: 355 LGPTPQQQPERGALAAFVVEGLHANDISAL-----LDASGICIRSGHHCT------QPLH 403 Query: 339 LAMGYSQKAALGGIRLTLGKQTTAADID 366 G S G R +L TT +ID Sbjct: 404 RHYGIS-----GSARASLSFTTTIEEID 426 >WH5701_13585 serine:pyruvate/alanine:glyoxylate aminotransferase Length = 402 Score = 37.7 bits (86), Expect = 7e-04, Method: Compositional matrix adjust. Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187 E+++ AL H ++++++ ++ G QP+ +G++ + + L D V G +PL + Sbjct: 146 EEIEQALASHRPAILAMVHAETSTGVCQPMEGIGELCRRYDCLLLLDTVTSLGAVPLHLD 205 Query: 188 TLPVDLLSLSSHK 200 VDL S K Sbjct: 206 AWGVDLAYSCSQK 218 >WH5701_13595 Pyridoxal-dependent decarboxylase family protein Length = 484 Score = 33.5 bits (75), Expect = 0.013, Method: Compositional matrix adjust. Identities = 29/122 (23%), Positives = 57/122 (46%), Gaps = 12/122 (9%) Query: 65 IFTSGGTEADNLAIMGVARCYT---TPQHMIISSVE-HSAISEPAKMLENWGWEITRLGV 120 + SGG+ ++ +A++ P+ +++ ++ H+++ + A ++ + RL + Sbjct: 157 VPASGGSLSNLMALVAARESRGLRGVPEAVVLCGLDSHTSLEKAAMVMGLPAAALHRLPL 216 Query: 121 NHQGRVNP-------EDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHT 173 + GR+ P E L AA LV+ G + G V P+ EL + + HG H Sbjct: 217 DGDGRLEPALVAEYLESLSAAGVPVIALVAT-AGTTVRGAVDPLVELASLCRRHGVWLHI 275 Query: 174 DA 175 DA Sbjct: 276 DA 277 Database: GCA_000153045.1P Posted date: Aug 27, 2016 11:08 PM Number of letters in database: 894,594 Number of sequences in database: 3346 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3346 Number of Hits to DB: 758,261 Number of extensions: 35337 Number of successful extensions: 131 Number of sequences better than 1.0: 6 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 894,594 Length adjustment: 82 Effective length of query: 300 Effective length of database: 620,222 Effective search space: 186066600 Effective search space used: 186066600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
1.436589586