BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000063505.1P
2533 sequences; 732,371 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SynWH7803_1263 nifS Cysteine desulfurase 238 4e-75
SynWH7803_2311 Cysteine desulfurase 207 2e-63
SynWH7803_2147 csd Selenocysteine lyase 100 2e-24
SynWH7803_0596 L-cysteine/cystine lyase 53 9e-09
SynWH7803_1345 spt, agt Alanine--glyoxylate transaminase 40 1e-04
>SynWH7803_1263 nifS Cysteine desulfurase
Length = 393
Score = 238 bits (607), Expect = 4e-75, Method: Compositional matrix adjust.
Identities = 143/361 (39%), Positives = 204/361 (56%), Gaps = 7/361 (1%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD ATTP RP + + Q WGNPSSLH +G AA LE R+++A L+ A
Sbjct: 9 IYLDACATTPLRPGVQQRMVAVQNQAWGNPSSLHCFGIAAAEALERTRVEIASLLGAEAN 68
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
++ TSG TE+ +L + G+A P ++IS+VEH A++ A+ L GW++ V+
Sbjct: 69 EVLITSGATESIHLGLRGLASSLP-PGRIVISAVEHPAVTAAAQALTPLGWQVCMAPVDA 127
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
QG + + L+ T LVSVI+GQSE+GT+QPI +G + + HG HTDA QV +
Sbjct: 128 QGTIQLDRFAELLQPPTRLVSVIWGQSEVGTLQPIQTIGALCRQHGIPLHTDATQVISQA 187
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG--VELMPLLGGGGQENGLRSGTQATPA 240
D TLPVDLL+ S+HK G G G L +R +L LL GGGQE+ LRSGT++
Sbjct: 188 LPDWSTLPVDLLTASAHKCGGPRGVGLLLVRGAWKQQLTALLTGGGQESNLRSGTESAVL 247
Query: 241 IAGFGVA----AELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFY 296
+AG A L + LRD L L G+ G RLPHHL+
Sbjct: 248 LAGMAEALSQIQRCGVDALSNSGNGIRRLRDALELQLGQRFGVATIGQPDQRLPHHLALL 307
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
L G+ +SG+ +VR L+ G+ +S+G+AC+SG+ + S +L AMG ++ +RL+L
Sbjct: 308 LRDRSGQPLSGRRMVRVLDREGLAVSSGSACSSGRDTDSSVLTAMGLPREMRRSSLRLSL 367
Query: 357 G 357
G
Sbjct: 368 G 368
>SynWH7803_2311 Cysteine desulfurase
Length = 392
Score = 207 bits (528), Expect = 2e-63, Method: Compositional matrix adjust.
Identities = 146/369 (39%), Positives = 206/369 (55%), Gaps = 14/369 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61
+ LD+ ATTP P + ++ Q WGN SS H G AA + +AR ++A +
Sbjct: 15 IQLDHQATTPCHPLVVKAMEPWWEQDWGNASSRQHRLGLTAAAAVASARSRLASHLGVEA 74
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQ----HMIISSVEHSAISEPAKMLENWGWEITR 117
+IFTSG TEA+NLA++G AR + H++ + EH A+ +P L+ G+ +T
Sbjct: 75 NDLIFTSGATEANNLALLGHARARAQAEGRCGHVVTLATEHHAVLDPMHQLQREGFALTV 134
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
L G ++ LK LR +T LVSV+ +EIG VQP+ EL ++ ++ G HTDA Q
Sbjct: 135 LHPKDTGLIDANALKGVLRDDTQLVSVMVANNEIGVVQPMRELAELCQSRGITLHTDAAQ 194
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQA 237
G L L+ L DL+SLS+HKL G G GAL R V + PL GGGQE LR GT
Sbjct: 195 AFGHLRLNRDELGCDLISLSAHKLNGPKGIGALVKRRNVSIQPLFWGGGQEQELRPGTLP 254
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFY 296
P I GF AA+LA +LET +L LRD+L+ + + P L G RL H+L+
Sbjct: 255 VPLIVGFAAAADLAHADLETRQDQLATLRDQLWQGLKSRHPQLRMNGAPEPRLAHNLNIT 314
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
+ +SG L R L + + S+G+AC+ G+ PS L A+G S++ A +RL+L
Sbjct: 315 V-----PDVSGVRLHRCLKRS-LACSSGSACSRGQ--PSHALQAIGLSRREAEASLRLSL 366
Query: 357 GKQTTAADI 365
G+ TT ADI
Sbjct: 367 GRTTTVADI 375
>SynWH7803_2147 csd Selenocysteine lyase
Length = 422
Score = 100 bits (248), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 116/393 (29%), Positives = 168/393 (42%), Gaps = 50/393 (12%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-----LHEWGNRAALILETARMQVAGLI 57
VYLD++AT+ +P+A+ L + N ++ H+ RA E AR VA +
Sbjct: 34 VYLDHAATS-QKPQAVL---DALRGYYSNDNANVHRGAHQLSARATEAFEGARAAVARFV 89
Query: 58 NAV-PESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKML-EN 110
A P+ ++FT TEA NL VAR + +++S +EH + P + L +
Sbjct: 90 GAAGPDEVVFTRNATEAINL----VARTWGDANLQAGDEVLLSVMEHHSNLVPWQQLAQR 145
Query: 111 WGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGAL 170
G + +G+ G ++ +DL+ L T LVS+++ + +G P+ E+ + GAL
Sbjct: 146 TGCVLRHVGITESGELDLDDLRQQLNKRTRLVSLVHISNTLGCRNPVEEIAALAHGVGAL 205
Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG-- 226
DA Q DV L VD L SSHKL G G G L+ R + + P LGGG
Sbjct: 206 MLVDACQSLAHQSTDVNQLGVDFLVGSSHKLCGPTGMGFLWARKDLLDAMPPFLGGGEMI 265
Query: 227 QENGLRSGTQA---------TPAI---AGFGVA-AELAAQELETETGRLILLRDRLFAIL 273
Q+ L T A TPAI G G A L A LE L LF L
Sbjct: 266 QDVFLDHSTWAALPHKFEAGTPAIGEAVGMGAAITYLEALGLEQIAAWEAQLTRYLFQQL 325
Query: 274 ADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLS 333
+ GL G + P + DG + + L+LAGI I +G C
Sbjct: 326 DSIEGLRILGPTPEQQPGRGALATFLVDG--VHANDIASMLDLAGICIRSGHHC------ 377
Query: 334 PSPILLAMGYSQKAALGGIRLTLGKQTTAADID 366
P+ + G R +L TT +ID
Sbjct: 378 CQPL-----HRHFKVTGSARASLSFTTTFEEID 405
>SynWH7803_0596 L-cysteine/cystine lyase
Length = 414
Score = 52.8 bits (125), Expect = 9e-09, Method: Compositional matrix adjust.
Identities = 84/327 (25%), Positives = 125/327 (38%), Gaps = 54/327 (16%)
Query: 2 QVYLDYSATTP-TRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60
+ Y +Y P P AI + Q P + W A + T R Q+A L
Sbjct: 26 KTYFNYGGQGPLPDPSLDAITASWKRIQQLGPFTTDVWPYIGAEVNST-RAQLATLCGVP 84
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPA-KMLENWGWEITRLG 119
P + + T L + G+ H++IS EH + ++ G + L
Sbjct: 85 PHRLALSENVTSGCVLPLWGLP--IREGDHVLISDCEHPGVVAACLELARRMGLSVHALP 142
Query: 120 VNH-QGRVNPED---------LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA 169
V H +G N +D L+ L T LV + + G PIA + + H A
Sbjct: 143 VKHLRGGRNDQDQTDAAVLEALEQQLTAKTRLVVLSHLLWNTGQRMPIAAVAAQLQQHPA 202
Query: 170 --LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGG---LGAGALYLRPGVELMPLLGG 224
DA Q G++P+D D+ + + HK G LG AL R E P L G
Sbjct: 203 QPFLLVDAAQSMGQIPVDEAAAAADIYAFTGHKWACGPEGLGGVALSERVLEESNPTLIG 262
Query: 225 GGQENGLRSGTQAT---------------------PAIAGFGVAAELAAQELETETG--- 260
LR T+A+ P +AG + + +LE E G
Sbjct: 263 W---RSLRDETRASLDDPHPFHADSRRFEVATSCVPLMAGLRCSLD----QLEHEGGPGQ 315
Query: 261 ---RLILLRDRLFAILADVPGLIPTGD 284
R+ LL +RL+ L+DVPG++P D
Sbjct: 316 RLERIRLLSERLWQELSDVPGVMPLLD 342
>SynWH7803_1345 spt, agt Alanine--glyoxylate transaminase
Length = 398
Score = 39.7 bits (91), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 24/91 (26%), Positives = 45/91 (49%), Gaps = 2/91 (2%)
Query: 129 EDLKAALRHNT-VLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
++L +AL+H+ ++++++ ++ G QP+ +G + + H L D V G +PL +
Sbjct: 144 DELDSALQHHKPAILAMVHAETSTGVCQPMEGVGDLCRKHDCLLLLDTVTSLGGVPLYLD 203
Query: 188 TLPVDL-LSLSSHKLYGGLGAGALYLRPGVE 217
VD+ S S L G G + P E
Sbjct: 204 EWKVDMAYSCSQKGLSCPPGLGPFTMGPRAE 234
Database: GCA_000063505.1P
Posted date: Aug 27, 2016 10:32 PM
Number of letters in database: 732,371
Number of sequences in database: 2533
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2533
Number of Hits to DB: 600,700
Number of extensions: 27184
Number of successful extensions: 82
Number of sequences better than 1.0: 6
Number of HSP's gapped: 73
Number of HSP's successfully gapped: 6
Length of query: 382
Length of database: 732,371
Length adjustment: 81
Effective length of query: 301
Effective length of database: 527,198
Effective search space: 158686598
Effective search space used: 158686598
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)