BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000025125.1P 1200 sequences; 386,913 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UCYN_04940 cysteine desulfurase family protein 483 e-171 UCYN_06120 cysteine desulfurase NifS 264 3e-85 UCYN_07350 cysteine desulfurase 86 1e-19 >UCYN_04940 cysteine desulfurase family protein Length = 386 Score = 483 bits (1244), Expect = e-171, Method: Compositional matrix adjust. Identities = 234/385 (60%), Positives = 296/385 (76%), Gaps = 5/385 (1%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 MQ+YLDYS+TTP RPE + I + TQ WGNPSS H+WG +A++ILE AR+Q+A I A Sbjct: 1 MQIYLDYSSTTPPRPEVVTYINQVCTQGWGNPSSTHQWGKKASMILERARIQIANSIKAN 60 Query: 61 P-ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 ESIIFTSGGTEA+NLAI+G A+ + QH+IISSVEHS+I++P K LE GWEITRL Sbjct: 61 DIESIIFTSGGTEANNLAILGTAKQHKRRQHIIISSVEHSSINKPIKFLETHGWEITRLP 120 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 V++QG+VNP DLKAA+R NTVL+S+IYGQSE+GT+QPI EL +I K++G LFHTDA Q Sbjct: 121 VDYQGKVNPFDLKAAIRKNTVLISIIYGQSEVGTLQPIKELSEIAKSYGILFHTDAAQAI 180 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 G+L ++VQ L +DLLS S+HK+YG G GALY+RPG + PL GGGQE G+RSGTQ+ Sbjct: 181 GKLAINVQDLNIDLLSFSAHKIYGTQGIGALYIRPGSIVSPLFHGGGQEKGIRSGTQSLA 240 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEA 299 IAGFG+A EL +E+ E+ RL+ LR++LF ++D P LI +GDK +RLPHH SF Sbjct: 241 TIAGFGLATELICREMIEESNRLMQLRNQLFKYMSDYPYLIVSGDKKYRLPHHASFIFSN 300 Query: 300 ADG----EKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLT 355 +KI+G+T+VR +NLAGIGISAG+AC+SGKL PSP+LLAMGYS A+ GIRLT Sbjct: 301 LSKNQKLKKITGQTIVRHMNLAGIGISAGSACHSGKLIPSPVLLAMGYSASDAVSGIRLT 360 Query: 356 LGKQTTAADIDWTGIVVKQILQRLI 380 LGK T+ DI WT IV+KQIL RL+ Sbjct: 361 LGKNTSKDDITWTAIVLKQILSRLL 385 >UCYN_06120 cysteine desulfurase NifS Length = 400 Score = 264 bits (674), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 155/383 (40%), Positives = 219/383 (57%), Gaps = 17/383 (4%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62 +YLD +ATT E + + L +GNPSS+H +G + + T R QVA L+ A P Sbjct: 5 IYLDNNATTQIDSEVLTEMMPYLNFYYGNPSSMHSFGGQVGKAVRTGREQVASLLGAEPS 64 Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122 IIFTS GTE DN AI +H+I + VEH AI K LE G+++T L V+ Sbjct: 65 EIIFTSCGTEGDNAAINAALIAQKNKRHIITTEVEHPAILSHCKQLERQGYQVTYLSVDQ 124 Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182 QG+++ +LK +L NT LVSV+Y +E G + PI E+G++ K +GALFH DAVQ G++ Sbjct: 125 QGQLDLNELKTSLTGNTALVSVMYANNETGVIFPIEEIGQMVKEYGALFHVDAVQAVGKI 184 Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAIA 242 PL+++T +D+L++S HK++ G GALY+R GV PL+ GG QE G R GT+ IA Sbjct: 185 PLNMKTSTIDMLTISGHKIHAPKGIGALYVRKGVRFRPLIIGGHQERGRRGGTENVSGIA 244 Query: 243 GFGVAAELAAQE-----LETETGRLILLRDRLF-AILADVPGLIPTGDKIHRLPHHLSFY 296 G AAELA LE E LRD L +IL +P + G+K +RLP+ + Sbjct: 245 ALGKAAELAQLNLPHIHLEQE------LRDYLEDSILNLIPDTVVNGNKTNRLPNTTNIG 298 Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356 + +GE I + +N GI S+G+AC SG L PS +L AM G IR +L Sbjct: 299 FKYIEGEAI-----LLSINKYGICASSGSACTSGSLEPSHVLRAMKVPYSVLHGSIRFSL 353 Query: 357 GKQTTAADIDWTGIVVKQILQRL 379 + TT ++ID ++ + RL Sbjct: 354 SRYTTKSEIDRVIEILPDTIGRL 376 >UCYN_07350 cysteine desulfurase Length = 413 Score = 85.5 bits (210), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 100/369 (27%), Positives = 157/369 (42%), Gaps = 28/369 (7%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61 +YLD +AT+ + ++ + N H RA E +R ++A IN+ Sbjct: 24 IYLDSAATSQKPTVVLDALRHYYEKDNANVHRGSHSLSIRATESYEASRDKLAKFINSND 83 Query: 62 -ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSV-EHSAISEPAKML-ENWGWEITRL 118 E IIFT TE+ NL P I++SV EH + P +++ + G I + Sbjct: 84 REEIIFTRNATESINLVAYSWGLNNLKPGDEILTSVMEHHSNLIPWQIIAKQTGANIKYI 143 Query: 119 GVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQV 178 + ++ + K+ L T LVS+++ + +G + P+ E+ +++ + GA DA Q Sbjct: 144 QLTENEDLDLDHFKSILSKKTKLVSIVHVSNMLGCMNPVEEITRLSHSVGAKVLIDACQS 203 Query: 179 AGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALY-LRPGVELMPLLGGGGQ---------- 227 P+DVQ + D L S HK+ G G G LY R +E MP GGG+ Sbjct: 204 VPHTPIDVQNIDCDWLVASGHKMCGPSGIGFLYGKREILESMPPFLGGGEMIDKVFLDHF 263 Query: 228 -----ENGLRSGTQATPAIAGFGVAAE-LAAQELETETGRLILLRDRLFAILADVPGL-I 280 + +GT A G A + L ++T L LF L ++P L I Sbjct: 264 TYGELPHKFEAGTPAIAEAIALGRAVDYLTEIGMDTIHSYEEQLSSYLFQRLEEIPDLRI 323 Query: 281 PTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLA 340 G KI + L + + I G L L+ GI I +G C P LL Sbjct: 324 YGGTKIKNGTGRAA--LASFNVNGIHGSDLSTLLDNEGIAIRSGDHCT----QPLHRLLQ 377 Query: 341 MGYSQKAAL 349 + S +A+L Sbjct: 378 ISGSARASL 386 Database: GCA_000025125.1P Posted date: Aug 27, 2016 10:58 PM Number of letters in database: 386,913 Number of sequences in database: 1200 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1200 Number of Hits to DB: 272,931 Number of extensions: 11067 Number of successful extensions: 37 Number of sequences better than 1.0: 4 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 4 Length of query: 382 Length of database: 386,913 Length adjustment: 77 Effective length of query: 305 Effective length of database: 294,513 Effective search space: 89826465 Effective search space used: 89826465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)

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