BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000012625.1P
2638 sequences; 722,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Syncc9605_2429 NifS-like aminotransferase class-V 240 2e-76
Syncc9605_1370 possible cysteine desulfurase (class-V aminotrans... 211 8e-65
Syncc9605_2279 cysteine desulfurases, SufS subfamily 96 5e-23
Syncc9605_1207 serine:pyruvate/alanine:glyoxylate aminotransferase 40 8e-05
Syncc9605_0659 glutamate-1-semialdehyde-2,1-aminomutase 29 0.33
>Syncc9605_2429 NifS-like aminotransferase class-V
Length = 354
Score = 240 bits (613), Expect = 2e-76, Method: Compositional matrix adjust.
Identities = 145/340 (42%), Positives = 206/340 (60%), Gaps = 11/340 (3%)
Query: 29 WGNPSSL-HEWGNRAALILETARMQVAGLINAVPESIIFTSGGTEADNLAIMGVARCYTT 87
WGNPSS H G A+ ++ AR Q+A + PE ++FTSG TEA+NLA++G AR
Sbjct: 9 WGNPSSRQHRLGLSASAAVKLARRQLAETLGVNPERLVFTSGATEANNLALLGHARALGK 68
Query: 88 PQHMIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYG 147
H+I + EH A+ +P + L+ G+ +T L G ++PE L+AA+ T LVSV+
Sbjct: 69 -AHLISVATEHHAVLDPLQQLQREGFSVTLLTPGPYGLISPEQLEAAITPETRLVSVMAA 127
Query: 148 QSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGA 207
+EIG +QP+ +LG + ++HG H+D Q G PL+ + VDLLSLS+HK+YG G
Sbjct: 128 NNEIGVLQPLEQLGAVCRSHGITLHSDGAQAFGTWPLNPDAVGVDLLSLSAHKIYGPKGI 187
Query: 208 GALYLRPGVELMPLLGGGGQENGLRSGTQATPAIAGFGVAAELAAQELETETGRLILLRD 267
GAL LR G+ + PL GGGQE GLR+GT T I GF AA LA QE + RL LRD
Sbjct: 188 GALVLREGIAIEPLQWGGGQEAGLRAGTLPTALIVGFAAAARLALQEQDQRNSRLQKLRD 247
Query: 268 RLFAILA-DVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAA 326
+L+ L +PG++ G RLPH+L+ L +G + L R L + S+G+A
Sbjct: 248 QLWEGLQRRLPGVLLNGALQPRLPHNLNISLPGVNGSR-----LHRALR-PHLACSSGSA 301
Query: 327 CNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADID 366
C++G +PS +L A+G S+ A +RL+LG+ TTA D+D
Sbjct: 302 CSNG--APSHVLQAIGRSRAEAEASLRLSLGRDTTATDVD 339
>Syncc9605_1370 possible cysteine desulfurase (class-V aminotransferase family)
Length = 390
Score = 211 bits (537), Expect = 8e-65, Method: Compositional matrix adjust.
Identities = 146/373 (39%), Positives = 212/373 (56%), Gaps = 9/373 (2%)
Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVP 61
++YLD +ATTP P IA++Q + + W NPSSLH G AA LE AR ++A P
Sbjct: 5 ELYLDAAATTPPLPAVIAVMQQVQQKAWANPSSLHGAGLAAAEALERARWRIAERFAVSP 64
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121
+ +I TSG TE+ +LA++G A P ++IS+VEH A+ A LE GW I V+
Sbjct: 65 DQLIVTSGATESVHLALLGSA-AGLVPGRLVISAVEHPAVVAAAHQLEALGWSIAEWPVD 123
Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181
+G V + L L T LVS+I Q E+G +QP++++ K + G + H+DA Q+ +
Sbjct: 124 REGVVRLDQLDRLLSAPTRLVSLIAAQGEVGALQPVSKIAKACRERGIVIHSDATQLVPQ 183
Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPAI 241
+ L VDLL+LS+HK G G G L PGVEL PL GGGGQE+GLRSGT+ +
Sbjct: 184 GCFAFERLGVDLLTLSAHKFCGPRGVGLLIRAPGVELSPLQGGGGQEHGLRSGTEPVALV 243
Query: 242 AGFGVA------AELAAQELET-ETGRLILLRDRLFAILADVPGLIPTGDKI-HRLPHHL 293
+G A + +Q + + ++ RD+L L ++P L G RLPHH+
Sbjct: 244 SGMADALMALPSFDPVSQPIPPGSSNQIRRQRDQLLERLLELPQLQLCGPHPDQRLPHHI 303
Query: 294 SFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIR 353
+ + DG+ + G+ LVR+L AG+ S+G+AC+SG + S +L AMG G+R
Sbjct: 304 ALLAKTVDGQPLPGRDLVRRLAAAGVACSSGSACSSGSSADSAVLTAMGIPGTERQSGLR 363
Query: 354 LTLGKQTTAADID 366
LTLG + D+D
Sbjct: 364 LTLGPWLSDQDLD 376
>Syncc9605_2279 cysteine desulfurases, SufS subfamily
Length = 426
Score = 95.9 bits (237), Expect = 5e-23, Method: Compositional matrix adjust.
Identities = 100/350 (28%), Positives = 150/350 (42%), Gaps = 31/350 (8%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAV- 60
+YLD++AT+ + + +Q + N H+ RA E AR A I+A
Sbjct: 38 IYLDHAATSQKPRQVLEALQHYYSCDNANVHRGAHQLSARATDAFEAARSTTAAFIDAAS 97
Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKML-ENWGWE 114
P I+FT +EA NL VAR + ++++ +EH + P ++L + G
Sbjct: 98 PREIVFTRNASEAINL----VARTWGDANLKQGDEILLTVMEHHSNLVPWQLLAQRTGCV 153
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G+ G ++ ED +A L T LVS+++ + +G P+ ++ GA D
Sbjct: 154 LRHVGITDSGELDLEDFRAQLNERTRLVSLVHISNSLGCCNPLDQVIPAAHAVGACVLVD 213
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPG-VELMPLLGGGG---QENG 230
A Q P+DV L D L SSHKL G G G L+ R +E MP GGG Q+
Sbjct: 214 ACQSLAHKPIDVVALDADFLVGSSHKLCGPTGMGFLWARESLLEAMPPFLGGGEMIQDVF 273
Query: 231 LRSGTQA---------TPAI---AGFGVAAE-LAAQELETETGRLILLRDRLFAILADVP 277
L T A TPAI G G A L LE L LFA L +
Sbjct: 274 LDHSTWAVLPHKFEAGTPAIGEAVGMGAAIRYLQMVGLEAIQAWEAQLTRHLFARLQAID 333
Query: 278 GLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAAC 327
G+ G + P + DG + + ++ +GI I +G C
Sbjct: 334 GVRVLGPTPDQQPERGALATFLVDG--VHANDIAALIDASGICIRSGHHC 381
>Syncc9605_1207 serine:pyruvate/alanine:glyoxylate aminotransferase
Length = 394
Score = 40.4 bits (93), Expect = 8e-05, Method: Compositional matrix adjust.
Identities = 25/91 (27%), Positives = 43/91 (47%), Gaps = 2/91 (2%)
Query: 129 EDLKAAL-RHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187
++L+ AL H ++++++ ++ G QP+ +G + + H L D V G +PL +
Sbjct: 140 DELEVALIEHKPAILAMVHAETSTGVCQPMEGVGDLCRKHDCLLLLDTVTSLGGVPLYID 199
Query: 188 TLPVDL-LSLSSHKLYGGLGAGALYLRPGVE 217
VDL S S L G G + P E
Sbjct: 200 EWKVDLAYSCSQKGLSCPPGLGPFTMGPRAE 230
>Syncc9605_0659 glutamate-1-semialdehyde-2,1-aminomutase
Length = 428
Score = 28.9 bits (63), Expect = 0.33, Method: Compositional matrix adjust.
Identities = 54/212 (25%), Positives = 82/212 (38%), Gaps = 51/212 (24%)
Query: 4 YLDY------SATTPTRPEAIAIIQTILTQ--QWGNPSSLHEWGNRAALILETARMQVAG 55
Y+DY + PE IA +Q + + +G P +L A M
Sbjct: 54 YIDYIGSWGPAICGHAHPEVIAALQEAIEKGTSFGAPCALEN---------TLAEM---- 100
Query: 56 LINAVP--ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGW 113
+I+AVP E + F + GTEA +A++ + R YT +I E L G
Sbjct: 101 VIDAVPSVEMVRFVNSGTEA-CMAVLRLIRAYTGRDKVI--KFEGCYHGHADMFLVKAGS 157
Query: 114 EITRLGVNHQGRV--------------NPEDLKAALRHNT-----VLVSVIYGQSEIGTV 154
+ LG+ V + E +KA N V++ I G + G +
Sbjct: 158 GVATLGLPDSPGVPRSTTANTLTAPYNDLEAVKALFAENPEAIAGVILEPIVGNA--GFI 215
Query: 155 QP----IAELGKITKTHGALFHTDAVQVAGRL 182
QP + L ++TK HGAL D V R+
Sbjct: 216 QPEPGFLEGLRELTKEHGALLVFDEVMTGFRI 247
Database: GCA_000012625.1P
Posted date: Aug 27, 2016 10:43 PM
Number of letters in database: 722,368
Number of sequences in database: 2638
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2638
Number of Hits to DB: 585,367
Number of extensions: 26199
Number of successful extensions: 95
Number of sequences better than 1.0: 5
Number of HSP's gapped: 89
Number of HSP's successfully gapped: 5
Length of query: 382
Length of database: 722,368
Length adjustment: 81
Effective length of query: 301
Effective length of database: 508,690
Effective search space: 153115690
Effective search space used: 153115690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)