BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000012525.1P 2661 sequences; 809,802 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Synpcc7942_1929 cysteine desulfurase 418 e-145 Synpcc7942_2558 cysteine desulfurase NifS 291 7e-96 Synpcc7942_1738 cysteine desulfurase 89 2e-20 Synpcc7942_B2661 cysteine desulfurase 80 2e-17 Synpcc7942_1255 L-cysteine/cystine lyase 69 7e-14 Synpcc7942_2310 conserved hypothetical protein, L-2,4-diaminobut... 33 0.014 Synpcc7942_2160 alanine-glyoxylate aminotransferase 32 0.029 Synpcc7942_0191 Serine--glyoxylate transaminase 29 0.31 C01a81ce2c89283cdfd9fd93bc96040c >Synpcc7942_1929 cysteine desulfurase Length = 386 Score = 418 bits (1075), Expect = e-145, Method: Compositional matrix adjust. Identities = 226/379 (59%), Positives = 272/379 (71%), Gaps = 2/379 (0%) Query: 1 MQVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 M YLDYSATT RPE + + +QWGN +SLH+WGNRAAL+LE +R QVA LI A Sbjct: 1 MSTYLDYSATTSMRPEVLERFTAVAQEQWGNAASLHQWGNRAALVLERSRQQVAALIQAE 60 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 PE+I F+SGGTE+DN AI+ P H+IIS+VEHSAI+ PA LE GW++TRL V Sbjct: 61 PEAIAFSSGGTESDNWAILSPYLADPRPGHLIISAVEHSAIARPAAWLEQRGWQVTRLPV 120 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 + GR+ P DL +A+R +T L+SVI+GQSE+GT+QPIAEL I + HG LFHTDAVQVAG Sbjct: 121 DRSGRIQPADLASAVRPDTRLISVIWGQSEVGTIQPIAELAAIAREHGILFHTDAVQVAG 180 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240 RLP+DVQ LP+DLLSLSSHKLYG G GALY+RPGVEL PLL GG QE+GLRSGT A Sbjct: 181 RLPIDVQRLPIDLLSLSSHKLYGPQGVGALYIRPGVELAPLLQGGNQESGLRSGTPPIAA 240 Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300 IA FG AA+LAA EL ET RL LRDRL A+LA P L TGD I RLPHH SF A Sbjct: 241 IAAFGEAAQLAAAELPHETARLQSLRDRLIAVLATEPRLRLTGDPIQRLPHHASFI--AR 298 Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360 G + + LVR +N G GIS G+ACNSG+ PSP+LLAMGYS + AL GIR +LG+ T Sbjct: 299 GGTTGTSQQLVRAMNRLGFGISGGSACNSGRSQPSPVLLAMGYSPQEALAGIRFSLGRST 358 Query: 361 TAADIDWTGIVVKQILQRL 379 A+++ I ++ L L Sbjct: 359 QLAEVEAAAIALRSALHSL 377 >Synpcc7942_2558 cysteine desulfurase NifS Length = 388 Score = 291 bits (746), Expect = 7e-96, Method: Compositional matrix adjust. Identities = 168/368 (45%), Positives = 226/368 (61%), Gaps = 8/368 (2%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSL-HEWGNRAALILETARMQVAGLINAVP 61 +YLDY ATTP + +Q +Q+GNP+S H +G AA ++ AR +A I A P Sbjct: 6 IYLDYHATTPVDSRVVTAMQPFWGEQFGNPASRSHRYGLEAAAAVQVARETLATAIAAQP 65 Query: 62 ESIIFTSGGTEADNLAIMGVARC-YTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGV 120 E +IF SG TEA+NLAI GVA + QH+I + EH A+ P + LE+ G+++T L V Sbjct: 66 EELIFCSGATEANNLAIKGVAETQHRKGQHLITVATEHQAVLSPCRYLESLGFQLTVLPV 125 Query: 121 NHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAG 180 N QG ++ E + AALR +T+LVSV+ +EIG +QPIA +G + + G +FH DA Q G Sbjct: 126 NSQGLISVEQVAAALRPDTILVSVMAANNEIGVLQPIAAIGALCRDRGVVFHCDAAQALG 185 Query: 181 RLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATPA 240 R+P +V L VDLLSLS HK+YG G G LY R GV + P L GG QE GLR+GT A Sbjct: 186 RIPFNVSDLNVDLLSLSGHKVYGPKGIGLLYRRSGVAIAPQLHGGSQEQGLRAGTLAPAL 245 Query: 241 IAGFGVAAELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPHHLSFYLEAA 300 I GF A ELA E TET RL LRDRL+ L + GL GD RLP +L+F +E Sbjct: 246 IVGFAQATELAIAEQPTETLRLQTLRDRLWQGLQAIGGLHLNGDPQQRLPSNLNFSIEGI 305 Query: 301 DGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGKQT 360 D + L++++ A I +S+G+AC+SG PS +L A+G S A +R+ LG+ T Sbjct: 306 DPSR-----LIQRIRGA-IAVSSGSACSSGNAEPSHVLTAIGRSPDLAHASLRIGLGRFT 359 Query: 361 TAADIDWT 368 A +ID T Sbjct: 360 IAEEIDRT 367 >Synpcc7942_1738 cysteine desulfurase Length = 419 Score = 88.6 bits (218), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 70/232 (30%), Positives = 113/232 (48%), Gaps = 12/232 (5%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHE----WGNRAALILETARMQVAGLIN 58 VYLD +AT+ +P A+ IQ+++ G S++H +A E AR +VA IN Sbjct: 30 VYLDNAATS-QKPRAV--IQSLVDYYEGYNSNVHRGVHTLSGKATDAYEGARQKVARFIN 86 Query: 59 AVPE-SIIFTSGGTEADNLAIMGVARCYTTP-QHMIISSVEHSAISEPAKMLE-NWGWEI 115 A E I++T +EA NL + +I+S++EH + P + + G + Sbjct: 87 AKTEQEIVYTRNASEAINLVAYSFGMNFLQAGDEIILSAMEHHSNLIPWQFVAAKTGAAL 146 Query: 116 TRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 +GV G+ + E ++ L T LVSV++ + +G P+ E+ ++ GA DA Sbjct: 147 KFVGVTETGQFDLEQFRSLLSDRTKLVSVVHVSNTLGCCNPVTEICQLAHAKGARVLIDA 206 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225 Q +DVQ + D L S HK+ G G LY + + ++ P LGGG Sbjct: 207 CQSLPHQAIDVQAIDCDWLVGSGHKMCAPTGIGFLYGKLDLLRQMPPFLGGG 258 >Synpcc7942_B2661 cysteine desulfurase Length = 626 Score = 80.5 bits (197), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 108/402 (26%), Positives = 171/402 (42%), Gaps = 54/402 (13%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-----LHEWGNRAALILETARMQVAGLI 57 V+ D +ATT +P+ ++ L+ + + +S HE R+ E AR QV + Sbjct: 243 VWFDNAATT-QKPQ---VVIDRLSHYYQHENSNIHRAAHELAARSTDAYEAAREQVRHFL 298 Query: 58 NAVP-ESIIFTSGGTEADNLAIMGVARCYTTPQ-----HMIISSVEHSAISEPAKMLE-N 110 NA E ++F G TEA NL VA+ + + ++I+ +EH A P + L Sbjct: 299 NAASTEEVVFVRGTTEAINL----VAKSWGSQNLKEGDEIVITWLEHHANIVPWQQLSAE 354 Query: 111 WGWEITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGAL 170 G + + V+ G+V ++ + L T +VS + +GT+ P E+ ++ +GA Sbjct: 355 TGARLRVVPVDDYGQVRLDEYQKLLSDRTKIVSFTQVSNALGTITPAKEIIELAHRYGAK 414 Query: 171 FHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGGQE 228 D Q L +DVQ L D S HK++G G G LY + + +P GG Sbjct: 415 VLLDGAQSVSHLAVDVQALDCDWFVFSGHKVFGPTGIGVLYGKQELLDATLPWQSGGNMI 474 Query: 229 NGL---RSGTQATPAI--AGFGVAAEL----AAQELETETG--RLILLRDRLF----AIL 273 + ++ Q PA AG G A+ AA E + G + L A+L Sbjct: 475 ADVTFEKTVYQPAPARFEAGTGNIADAVGLGAALEYVQKIGLEAIAAYEHELLVHGTALL 534 Query: 274 ADVPGLIPTGDKIHRLPHHLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLS 333 + +PGL G H+ LSF LE E I + LN GI + AG C Sbjct: 535 SQIPGLRLIGTAPHKA-AVLSFVLEGFSPEAIG-----QALNREGIAVRAGHHCA----- 583 Query: 334 PSPILLAMGYSQKAALGGIRLTLGKQTTAADIDWTGIVVKQI 375 PIL G +R +L T +++ +++I Sbjct: 584 -QPILRRFGLETT-----VRPSLAFYNTFEELETLAAAIRRI 619 >Synpcc7942_1255 L-cysteine/cystine lyase Length = 387 Score = 68.9 bits (167), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 64/220 (29%), Positives = 95/220 (43%), Gaps = 11/220 (5%) Query: 2 QVYLDYSATTPTRPEAIAIIQTIL--TQQWGNPSSLHEWGNRAALILETARMQVAGLINA 59 + Y +Y P A+ IQT +QQ G P SL G R I + R +A L+ Sbjct: 19 KAYFNYGGQGPLSTAALTAIQTAFADSQQLG-PFSLAT-GQRVEQISDRLRTDLAALLQT 76 Query: 60 VPESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEH-SAISEPAKMLENWGWEITRL 118 E+I T + N+A+ GV + ++I+ EH S I+ + + +G E+ Sbjct: 77 QSENITLTENVSVGCNIALWGVD--WQAGDRLLITDCEHPSVIATAQAIAQRFGVELDIW 134 Query: 119 GVNHQ---GRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDA 175 + G+ E A L T L ++ + GTV P+A + + L D Sbjct: 135 PLQAAVVAGQDLTEAFAAQLNSRTRLAAISHVLWNYGTVLPLAAIAEQAHRQDCLLLVDG 194 Query: 176 VQVAGRLPLDVQTLPVDLLSLSSHK-LYGGLGAGALYLRP 214 Q G LPLD+ VD + + HK L G G G LY RP Sbjct: 195 AQSVGLLPLDLPATGVDFYAFTGHKWLCGPEGVGGLYSRP 234 >Synpcc7942_2310 conserved hypothetical protein, L-2,4-diaminobutyrate decarboxylase Length = 489 Score = 33.5 bits (75), Expect = 0.014, Method: Compositional matrix adjust. Identities = 22/91 (24%), Positives = 41/91 (45%), Gaps = 6/91 (6%) Query: 91 MIISSVEHSAISEPAKMLENWGWEITRLGVNHQGRVN----PEDLKAALRHNTVLVSVIY 146 + S H++I + A +L + + + +GR+ E + +A R ++ Sbjct: 184 LFASEAAHTSIQKAAMLLGLGSQAVIPIATDRRGRMQVAALTEAIASARRQGQRPFCLVA 243 Query: 147 --GQSEIGTVQPIAELGKITKTHGALFHTDA 175 G + G++ P+ EL I +T G FH DA Sbjct: 244 TAGTTVTGSIDPLPELSAIARTEGLWFHVDA 274 >Synpcc7942_2160 alanine-glyoxylate aminotransferase Length = 384 Score = 32.3 bits (72), Expect = 0.029, Method: Compositional matrix adjust. Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 1/72 (1%) Query: 130 DLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQT 188 +L+ AL H ++++++ ++ G QP+A +G + + L D V G +P + Sbjct: 139 ELRQALEEHRPQILALVHAETSTGAEQPLAGVGDLCREFDCLLLIDTVTSLGAVPTLLDE 198 Query: 189 LPVDLLSLSSHK 200 VDL S K Sbjct: 199 WGVDLAYSCSQK 210 >Synpcc7942_0191 Serine--glyoxylate transaminase Length = 382 Score = 29.3 bits (64), Expect = 0.31, Method: Compositional matrix adjust. Identities = 21/101 (20%), Positives = 46/101 (45%), Gaps = 4/101 (3%) Query: 106 KMLENWGWEITRLGVNHQGRVNPEDLKAALRHNT----VLVSVIYGQSEIGTVQPIAELG 161 K+ + +G ++ + ++PE ++A L +T V + + ++ G + + + Sbjct: 95 KLAQAFGLDVDLVQAPWGKPLDPEAIRAKLEADTDKQIKAVILTHSETSTGVINDLETIS 154 Query: 162 KITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKLY 202 + HGAL DAV G + + +D+++ S K Y Sbjct: 155 GYIRAHGALSIVDAVTSLGAANVPIDAWGLDVVASGSQKGY 195 Database: GCA_000012525.1P Posted date: Aug 27, 2016 10:53 PM Number of letters in database: 809,802 Number of sequences in database: 2661 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2661 Number of Hits to DB: 651,844 Number of extensions: 29061 Number of successful extensions: 116 Number of sequences better than 1.0: 9 Number of HSP's gapped: 109 Number of HSP's successfully gapped: 9 Length of query: 382 Length of database: 809,802 Length adjustment: 82 Effective length of query: 300 Effective length of database: 591,600 Effective search space: 177480000 Effective search space used: 177480000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
0.81807013