cyanobase / GCA_001277295.1 - AA650

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= AA650_17280 cysteine desulfurase (382 letters) Database: GCA_000012465.1P 2163 sequences; 535,974 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PMN2A_1538 NifS-like aminotransferase class-V 229 6e-72 PMN2A_0323 possible cysteine desulfurase (class-V aminotransfera... 207 1e-63 PMN2A_1433 cysteine desulfurase 90 5e-21 PMN2A_1748 L-cysteine/cystine lyase-like protein 40 1e-04 PMN2A_0142 alanine-glyoxylate aminotransferase apoenzyme 37 8e-04 85001e9ba6440d6732da992d8511bfb3

>PMN2A_1538 NifS-like aminotransferase class-V Length = 382 Score = 229 bits (584), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 138/368 (37%), Positives = 208/368 (56%), Gaps = 14/368 (3%) Query: 5 LDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEW-GNRAALILETARMQVAGLINAVPES 63 D+ ++TP + + + + WGNPS+ + G A+ +E +R ++A +N P+ Sbjct: 8 FDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASYLNINPKR 67 Query: 64 IIFTSGGTEADNLAIMGVARCYT----TPQHMIISSVEHSAISEPAKMLENWGWEITRLG 119 +IFTSG TEA+NL ++G AR P H+I S EH A+ +P + L+ G+ +T L Sbjct: 68 LIFTSGATEANNLGLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQKEGFRLTELH 127 Query: 120 VNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVA 179 N +G +N E L A +T LVSV+ +EIG +QPI ++G K G FH+DA Q Sbjct: 128 PNKEGLINIEQLSEAFEKDTFLVSVMAANNEIGVLQPIGDIGSFCKRKGIAFHSDAAQAF 187 Query: 180 GRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239 G L LD +DL+SLS+HK+YG G GAL +R G L P GGGQE GLRSGT P Sbjct: 188 GYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLRSGTLPVP 247 Query: 240 AIAGFGVAAELAAQELETETGRLILLRDRLFAIL-ADVPGLIPTGDKIHRLPHHLSFYLE 298 I GF A E+ + + RL+ R+ L + L ++ GLI G RLPH+L+ Sbjct: 248 LIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPHNLNITFP 307 Query: 299 AADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTLGK 358 G ++ G+ +R+ I ++G+AC++G+ S +L +G S+K A IR+++G+ Sbjct: 308 GVKGSQLHGQ--LRRF----IFCTSGSACSNGE--ASHVLQEIGLSKKDAEASIRMSIGR 359 Query: 359 QTTAADID 366 TT DI+ Sbjct: 360 NTTEKDIN 367 >PMN2A_0323 possible cysteine desulfurase (class-V aminotransferase family) Length = 393 Score = 207 bits (527), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 141/387 (36%), Positives = 212/387 (54%), Gaps = 10/387 (2%) Query: 2 QVYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVP 61 +YLD SATTP + I ++ I + WGNPSS+H+ G A ILE +R+ +A + A Sbjct: 3 DIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKASS 62 Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVN 121 + + FTSG TE++ LA+ V+ + ++ISSVEH +I+ A L N GW+I V+ Sbjct: 63 DELFFTSGATESNYLALKAVSNNLDRGR-IVISSVEHPSINLIANQLRNEGWDIKYWPVD 121 Query: 122 HQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGR 181 G ++ + L+ L T LVS+I+GQS IG++QPI +G K LFHTDA QV Sbjct: 122 SYGIIDLDLLEEVLSPPTKLVSIIWGQSVIGSIQPINLIGMECKKRNILFHTDATQVLPC 181 Query: 182 LPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVE--LMPLLGGGGQENGLRSGTQATP 239 +D L VD+LS S+HKL G G G L LR G++ LM G + +RSGT++ P Sbjct: 182 GLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESVP 241 Query: 240 AIAGFGVAAELAAQELETETGRLIL-------LRDRLFAILADVPGLIPTGDKIHRLPHH 292 IAGF A +L + +E + + + + L L + L G RLP++ Sbjct: 242 LIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSLLKRKLINNKHLTFIGPDKERLPNN 301 Query: 293 LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGI 352 LSF I G+ +VR L+ G+ IS+G+AC+S P+PIL+A+ + G+ Sbjct: 302 LSFLCHTEAMIPIKGREIVRLLSKHGVYISSGSACSSSSQGPNPILVAINVDKTFQESGL 361 Query: 353 RLTLGKQTTAADIDWTGIVVKQILQRL 379 R+T+G + DI ++ + L+ L Sbjct: 362 RITIGPWISNDDITSVSNIIFESLKSL 388 >PMN2A_1433 cysteine desulfurase Length = 416 Score = 89.7 bits (221), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 66/230 (28%), Positives = 113/230 (49%), Gaps = 8/230 (3%) Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGN-PSSLHEWGNRAALILETARMQVAGLINAVP 61 +YLD++AT+ + I ++ + Q N H+ A E +R A IN+ Sbjct: 28 IYLDHAATSQKPKQVIDSLKKYYSFQNANVHRGAHQLSALATEKFENSRKLTANFINSKN 87 Query: 62 ES-IIFTSGGTEADNLAIMGVARCYTTPQH--MIISSVEHSAISEPAKML-ENWGWEITR 117 E IIFT TEA NL Y ++ ++IS +EH + P +++ + ++ Sbjct: 88 EKEIIFTRNATEAINLVAYTWGN-YELQENDEILISLMEHHSNIVPWQLIAKAKKCKLIY 146 Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177 + ++ G ++ +D K L T +VS+++ + +G PI E+ + G+L DA Q Sbjct: 147 INIDKNGELDFDDFKKKLSDKTKIVSLVHVSNTLGCCNPIEEISSLAHQKGSLVLLDACQ 206 Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGG 225 +D++ L +D L+ SSHKL G G G L+ R + ++ P LGGG Sbjct: 207 SLAHKQVDIKKLGIDFLAGSSHKLCGPTGIGFLWGREEILKKIPPFLGGG 256 >PMN2A_1748 L-cysteine/cystine lyase-like protein Length = 400 Score = 39.7 bits (91), Expect = 1e-04, Method: Compositional matrix adjust. Identities = 58/240 (24%), Positives = 96/240 (40%), Gaps = 20/240 (8%) Query: 2 QVYLDYSATTPTRPEAI-AIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAV 60 + Y +Y P +++ AI + T Q P + H W ++ T + +A + + Sbjct: 16 KAYFNYGGQGPLPTQSLNAITSSWQTIQKLGPFTNHVWPYITKEVITTKNL-IAEICSIH 74 Query: 61 PESIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKML---ENWGWEITR 117 P+ I FT T L ++G+ ++ ++++S EH I K L +N I Sbjct: 75 PKRIAFTENVTSGCVLPLLGLP--FSDGDNLLLSDCEHPGIVAACKELARKKNLTIAILP 132 Query: 118 LGVNHQGRVNPED--------LKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGA 169 + G ++ + L+ NT LV + + G + PI + K K H + Sbjct: 133 VSKLCNGNDKKDETYNSVLKLIDEYLQKNTKLVVLSHLLWNTGQIMPIELISKRLKEHSS 192 Query: 170 --LFHTDAVQVAGRLPLDVQTLPVDLLSLSSHKL-YG--GLGAGALYLRPGVELMPLLGG 224 DA Q +P D+ + + HK YG GLGA L R E P L G Sbjct: 193 KPYLLVDAAQSFCHIPSKGACDTADIYAFTGHKWAYGPEGLGAVVLSARVLEESSPTLIG 252 >PMN2A_0142 alanine-glyoxylate aminotransferase apoenzyme Length = 412 Score = 37.0 bits (84), Expect = 8e-04, Method: Compositional matrix adjust. Identities = 17/73 (23%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Query: 129 EDLKAALR-HNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQ 187 ++++ AL+ H ++++++ ++ G QP+ +G + + + L D V G +PL + Sbjct: 165 QEIEDALKKHTPAVLAIVHAETSTGVCQPMDGIGDLCRKYNCLLLVDTVTSLGGVPLYLD 224 Query: 188 TLPVDLLSLSSHK 200 +DL S K Sbjct: 225 EWKIDLAYSCSQK 237 Database: GCA_000012465.1P Posted date: Aug 27, 2016 10:44 PM Number of letters in database: 535,974 Number of sequences in database: 2163 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2163 Number of Hits to DB: 380,505 Number of extensions: 15554 Number of successful extensions: 65 Number of sequences better than 1.0: 6 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 535,974 Length adjustment: 79 Effective length of query: 303 Effective length of database: 365,097 Effective search space: 110624391 Effective search space used: 110624391 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)