BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= AA650_17280 cysteine desulfurase
(382 letters)
Database: GCA_000011465.1P
1948 sequences; 498,304 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PMM0170 NifS-like aminotransferase class-V 218 1e-67
PMM0887 nifS possible cysteine desulfurase (class-V aminotransfe... 198 3e-60
PMM0070 putative cysteine desulfurase or selenocysteine lyase 97 2e-23
PMM0919 spt, agt serine:pyruvate/alanine:glyoxylate aminotransfe... 40 9e-05
PMM1075 petH ferredoxin-NADP oxidoreductase (FNR) 29 0.20
>PMM0170 NifS-like aminotransferase class-V
Length = 382
Score = 218 bits (554), Expect = 1e-67, Method: Compositional matrix adjust.
Identities = 129/372 (34%), Positives = 202/372 (54%), Gaps = 14/372 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEW-GNRAALILETARMQVAGLINAVP 61
+ LDY ++TP E + + ++ + NPSS G A+ +LE +R ++ +
Sbjct: 7 ILLDYQSSTPCLEEVVNSMAPYWSEIFSNPSSKSNLAGINASAVLEVSREKIQEYLFLKK 66
Query: 62 ESIIFTSGGTEADNLAIMGVARCYTTPQ----HMIISSVEHSAISEPAKMLENWGWEITR 117
+ +IFTSG TE++NLA++G AR + H+I EH A+ EP L G+ IT
Sbjct: 67 KKVIFTSGATESNNLALLGFARRHFKETEHYGHIITLKTEHKAVLEPLNQLRKEGFHITE 126
Query: 118 LGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQ 177
+ G V+ E + +R +T LVS++ +EIG +QP+ E+ +I + + H+D Q
Sbjct: 127 ISPEKDGLVSEEKFVSCIRDDTFLVSIMMANNEIGVIQPLKEISEICGSRDIVLHSDYAQ 186
Query: 178 VAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQA 237
G L D ++L++SSHK+YG G G L + +EL PL+ GGGQE GLRSGT
Sbjct: 187 CLGFLEFDSLDSVANMLTISSHKIYGPKGVGILLIDRDIELQPLVLGGGQEFGLRSGTLP 246
Query: 238 TPAIAGFGVAAELAAQELETETGRLILLRDRLFA-ILADVPGLIPTGDKIHRLPHHLSFY 296
P I GF A E+A + + + RD+L +L + G+ G RLPH+L+
Sbjct: 247 LPLIVGFTKAIEIAVLNQKKNINKFLFYRDKLLKGLLGNNSGVEINGSMKERLPHNLNLT 306
Query: 297 LEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGGIRLTL 356
+ +G K+ K+L + I S+G+AC++G PS +LLA+G S K A +RL++
Sbjct: 307 VLDVNGSKLH-KSLKSK-----IICSSGSACSNG--VPSHVLLALGRSFKEAEASLRLSI 358
Query: 357 GKQTTAADIDWT 368
G TT DI+ +
Sbjct: 359 GLMTTKEDIEKS 370
>PMM0887 nifS possible cysteine desulfurase (class-V aminotransferase
family)
Length = 390
Score = 198 bits (504), Expect = 3e-60, Method: Compositional matrix adjust.
Identities = 125/375 (33%), Positives = 188/375 (50%), Gaps = 14/375 (3%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSSLHEWGNRAALILETARMQVAGLINAVPE 62
+YLD ++TTP + I + W N SS +++G R A LE R+++A ++NA E
Sbjct: 6 IYLDNASTTPLSKNVLNKINSTYKNYWSNSSSTYKYGIRCATYLEKIRLKIAKILNANTE 65
Query: 63 SIIFTSGGTEADNLAIMGVARCYTTPQHMIISSVEHSAISEPAKMLENWGWEITRLGVNH 122
I+FTSG +E+ +L ++ Y + ++IS+VEH A A L+ GWEI + VN
Sbjct: 66 DIVFTSGSSESTSLVFSNLSDKYKCGR-VVISNVEHQATIISANKLKRKGWEIYKWPVNR 124
Query: 123 QGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRL 182
G +N ++ +++NT LVS+I+GQSE+GT+QP+ +G K LFH D Q+
Sbjct: 125 DGIINISNINEVIKNNTNLVSLIWGQSEVGTLQPVQLVGTKCKEFDILFHIDGTQILNNG 184
Query: 183 PLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGVELMPLLGGG----GQENGLRSGTQAT 238
+ + L DLLSLS+HK G G G L L LL QE +R GT
Sbjct: 185 IFNWKELNCDLLSLSAHKFGGPKGIGLLLTNSKSRL--LLKNNDISLSQEYSIRQGTTPL 242
Query: 239 PAIAGFGVA-------AELAAQELETETGRLILLRDRLFAILADVPGLIPTGDKIHRLPH 291
P IAG + + + E E + +LL + + P + TG RLP+
Sbjct: 243 PLIAGMYQSIKNIKGRIKFNSYNAEFENNKKVLLSKYFINKITNNPNIKITGSSTERLPN 302
Query: 292 HLSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACNSGKLSPSPILLAMGYSQKAALGG 351
H+SF L E I ++ ++ + IS+G+AC+S S L MGY
Sbjct: 303 HISFLLFNKKLEPIKAYKVINYMSDNNVAISSGSACSSSSGKHSLTLENMGYDSNQLYSN 362
Query: 352 IRLTLGKQTTAADID 366
IR++LG +DID
Sbjct: 363 IRVSLGSMNKKSDID 377
>PMM0070 putative cysteine desulfurase or selenocysteine lyase
Length = 425
Score = 97.1 bits (240), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 107/398 (26%), Positives = 170/398 (42%), Gaps = 60/398 (15%)
Query: 3 VYLDYSATTPTRPEAIAIIQTILTQQWGNPSS-LHEWGNRAALILETARMQVAGLINAVP 61
+YLD++ATT + + I N H+ +A E +R +A +NA
Sbjct: 37 IYLDHAATTQKPIQVLEKIDEYYKNFNANVHRGAHQLSAKATEEFENSRSLIAKYVNAYS 96
Query: 62 -ESIIFTSGGTEADNLAIMGVARCY-----TTPQHMIISSVEHSAISEPAKMLE-NWGWE 114
+ IIFT TEA NL VAR + +++S +EH + P +M+ +
Sbjct: 97 TKEIIFTRNATEAINL----VARSWGEFTLKENDEILLSIMEHHSNIVPWQMVAAKNKCK 152
Query: 115 ITRLGVNHQGRVNPEDLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTD 174
+ +G++ GR++ D K+ L T LVS+++ + +G PI E+ K+ + G+L D
Sbjct: 153 LKFVGIDQDGRLDINDFKSKLTTKTKLVSLLHISNTLGCCNPIKEITKLARDKGSLVLLD 212
Query: 175 AVQVAGRLPLDVQTLPVDLLSLSSHKLYGGLGAGALYLRPGV--ELMPLLGGGG--QENG 230
A Q LD+ L +D L+ S HKL G G G L+ R + ++ P GGG Q+
Sbjct: 213 ACQSLAHQKLDINELGIDFLAGSGHKLCGPTGIGFLWSRKEILEKIPPFFGGGEMIQDVF 272
Query: 231 LRSGTQA---------TPAIA-GFGVAAELAA---------QELETETGRLILLRDRLFA 271
T A TPAIA G+AA + E E E + LF
Sbjct: 273 EEKSTWAELPHKFEAGTPAIAEAIGLAAAINYINNIGLDRISEYEKEITKY------LFK 326
Query: 272 ILADVPGLIPTGDKIHRLPHH---LSFYLEAADGEKISGKTLVRQLNLAGIGISAGAACN 328
L + L G P+ +FY+ ++I + L+ GI I +G C
Sbjct: 327 KLKQIEDLEIIGPSPKIDPNRAALATFYV-----KEIHSNDIAEILDSKGICIRSGHHC- 380
Query: 329 SGKLSPSPILLAMGYSQKAALGGIRLTLGKQTTAADID 366
P+ +G + A R+++ TT DI+
Sbjct: 381 -----CQPLHRHIGINSTA-----RISMNFTTTKEDIN 408
>PMM0919 spt, agt serine:pyruvate/alanine:glyoxylate aminotransferase
Length = 394
Score = 39.7 bits (91), Expect = 9e-05, Method: Compositional matrix adjust.
Identities = 26/100 (26%), Positives = 43/100 (43%), Gaps = 10/100 (10%)
Query: 140 VLVSVIYGQSEIGTVQPIAELGKITKTHGALFHTDAVQVAGRLPLDVQTLPVDLLSLSSH 199
V+ ++++ ++ G +QP+ +G + + + LF DAV G L L + +DL S
Sbjct: 152 VIFAIVHAETSTGVLQPLEGIGDLCRKNNCLFLVDAVTSLGALELFIDDWKIDLAYSCSQ 211
Query: 200 KLYGGLGAGALYLRPGVELMPLLGGGGQENGLRSGTQATP 239
K L PG L P E+ L + T P
Sbjct: 212 K--------GLSCPPG--LSPFTMNKRAEDKLSARTSKVP 241
>PMM1075 petH ferredoxin-NADP oxidoreductase (FNR)
Length = 370
Score = 28.9 bits (63), Expect = 0.20, Method: Compositional matrix adjust.
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
Query: 278 GLIPTGDKIHRLPHHLSFYLEAA--DGEKISGKTL---VRQLNLAGIGISAGAACNS 329
G++P G+ + PH L Y A+ G+ G T+ VRQL G + C+S
Sbjct: 131 GIMPEGEDANGKPHKLRLYSIASTRHGDDFEGNTVSLCVRQLQYEKDGETINGVCSS 187
Database: GCA_000011465.1P
Posted date: Aug 27, 2016 10:52 PM
Number of letters in database: 498,304
Number of sequences in database: 1948
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1948
Number of Hits to DB: 333,315
Number of extensions: 12830
Number of successful extensions: 48
Number of sequences better than 1.0: 5
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 5
Length of query: 382
Length of database: 498,304
Length adjustment: 78
Effective length of query: 304
Effective length of database: 346,360
Effective search space: 105293440
Effective search space used: 105293440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)