cyanobase / GCA_000153285.1 - WH7805

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase (462 letters) Database: GCA_001264245.1P 5974 sequences; 1,430,872 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VL20_6472 UDP-N-acetylmuramoylalanine-D-glutamate ligase 350 e-116 VL20_4414 UDP-N-acetylmuramate-alanine ligase 52 4e-08 VL20_1487 UDP-N-acetylmuramoylalanyl-D-glutamate-26- diaminopime... 48 9e-07 VL20_3895 UDP-N-acetylmuramoylalanyl-D-glutamyl-26- diaminopimel... 41 2e-04 VL20_313 putative amino acid ligase found clustered with an amid... 39 8e-04 VL20_4628 Dihydrofolate synthase 31 0.16 VL20_2889 Amine oxidase 29 0.73 Ad8c8b0829492ae7dde007a49afa3894

>VL20_6472 UDP-N-acetylmuramoylalanine-D-glutamate ligase Length = 449 Score = 350 bits (897), Expect = e-116, Method: Compositional matrix adjust. Identities = 202/458 (44%), Positives = 281/458 (61%), Gaps = 16/458 (3%) Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60 M+++ ++GLGRSG AAR L+ G+ V + +R D P+L+ L ++ I + LG L L Sbjct: 1 MAKAAIIGLGRSGIAAARCLKRDGWQVTLSDRSDSPSLQATKTNLEREGIIVNLGQNLSL 60 Query: 61 DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNG 120 ++ D +V+SPG+ WD L R G GE+ +AW L+ PW+GITGTNG Sbjct: 61 EAS----DLPNLIVVSPGVPWDAPILITAREKGIETIGELELAWRYLQSSPWLGITGTNG 116 Query: 121 KTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAA 180 KTT T L + AGL AP GN+GY+A ELAL+ K DW++ E+SSYQIE++ Sbjct: 117 KTTTTALCAAIFQKAGLNAPSCGNIGYAACELALKA-----DKYDWIIAEISSYQIESSR 171 Query: 181 EVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQ-HCGHWSRS 239 ++SP+IGIWTT TPDHL RH T+E Y IK LL+RS+ +LN DD +RQ W ++ Sbjct: 172 DLSPKIGIWTTFTPDHLSRHQTLENYYQIKASLLQRSDRQILNGDDPYLRQIGVSQWQQA 231 Query: 240 CIRWVSCGDHSPEGGNPQLTVNAQ-GWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAAL 298 W S + G+P V Q W+ + + P + L + G HN+QN+L+ AAA Sbjct: 232 --YWTSVQGKAALLGDPSRGVYLQDNWIVAFGELIAPVNLLKMVGSHNQQNLLMAVAAAR 289 Query: 299 AAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLL 358 AG+ I A+ F GV HRLE + + G+ NDSKATNYDAAAVGL+SV +P +L+ Sbjct: 290 LAGIEKKAITEAIATFPGVAHRLEYICTHKGLDFINDSKATNYDAAAVGLQSVPSPAILI 349 Query: 359 AGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD-LDSAVSQ 417 AGG+ K GD W+ I+++VA V+L G+ A + A ++++G+ C + +D AV + Sbjct: 350 AGGEAKAGDDRAWIAEIKAKVATVLLIGDAAADFARRLQSAGYQD--YECVETMDRAVQR 407 Query: 418 ALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455 A L +A VLLSPACASFDQY +FE RGDHFRQL Sbjct: 408 AAELGTAKEAKVVLLSPACASFDQYQSFEHRGDHFRQL 445 >VL20_4414 UDP-N-acetylmuramate-alanine ligase Length = 475 Score = 52.4 bits (124), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 67/275 (24%), Positives = 106/275 (38%), Gaps = 30/275 (10%) Query: 72 EVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHV 131 +V+ S I + A R G+ + V + I + GT+GKTT + L+ +V Sbjct: 82 QVICSTAINDHNKEYRAARDLGYPIFHRSDVLAALIADYDSIAVAGTHGKTTTSSLIGYV 141 Query: 132 LAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEVSPRIGIWTT 191 L AGL+ + A E + QG ++V E + + +P+IG+ T Sbjct: 142 LLEAGLDPTIIVGGEVDAWEGNARLGQGR-----FLVAEADESD-GSLTKHAPKIGVITN 195 Query: 192 LTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWSRSCIRWVSCGDHSP 251 + DH + + + R + +I V D I +H R I + P Sbjct: 196 IELDHPDHYQNLSEVIDTFREFANQCQILVACLDCDTIAEHF----RPTISY----SLDP 247 Query: 252 EGGNPQLTVNAQGWVSRGEQHLFPAD-----------SLPLPGEHNRQNMLLVTAAALAA 300 E G TV+ + EQ + A + +PG+HN N L V A Sbjct: 248 EKG-ADYTVSEIIY----EQGMMKASVWEKGSYLGQMEVKIPGQHNISNALAVVAIGRYL 302 Query: 301 GVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335 G+ + I A+ F G R E G GI +D Sbjct: 303 GLEFAVIADAIATFAGAKRRFEHKGEANGITFIDD 337 >VL20_1487 UDP-N-acetylmuramoylalanyl-D-glutamate-26- diaminopimelate ligase Length = 496 Score = 48.1 bits (113), Expect = 9e-07, Method: Compositional matrix adjust. Identities = 61/222 (27%), Positives = 96/222 (43%), Gaps = 30/222 (13%) Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYS---------AAELALEVLQGTRPKPDWVVMEM 171 HL+++ L+ AG+ + G + G+ A EL + + + VME+ Sbjct: 126 HLIEYFLSQAGIPTALFGTLYTRWQGFQETANHTTPFANELQASLAKAVSAGNEIAVMEV 185 Query: 172 SSYQIEAAAEV---SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNAD 225 SS+ + A V + + ++T LT DHL+ H +E Y A K L + ++N D Sbjct: 186 SSHAL-AQGRVQGCTFPVTVFTNLTRDHLDYHLDMEDYFAAKAKLFSPDYLQGKAIINLD 244 Query: 226 DADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQG-----WVSRGEQHLFPADSLP 280 D ++ S + S D S + L G RGE A + P Sbjct: 245 DVYGQRLAASLSPQQVLTYSVSDASADFYTKDLQYQPTGVEGVMVTPRGEI----AFASP 300 Query: 281 LPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322 L G++N N+L AA L GV T+ A + F+GVP R+E Sbjct: 301 LVGQYNLANLLAAVAAVLECGVDLETVIAQIPQFSGVPGRME 342 >VL20_3895 UDP-N-acetylmuramoylalanyl-D-glutamyl-26- diaminopimelate-D-alanyl-D-alanine ligase Length = 454 Score = 40.8 bits (94), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 60/224 (26%), Positives = 88/224 (39%), Gaps = 32/224 (14%) Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGN----VGYSAAELALEVLQG 159 W IP I ITG+ GKTT L+ VLA G N +G L L+ Sbjct: 104 WRNQFTIPVIAITGSVGKTTTKELIASVLATRGKVHKTLANYNNEIGVPKTLLELD---- 159 Query: 160 TRPKPDWVVMEMSSY---QIEAAAEVS-PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLE 215 D V+EM +I A+++ P I + T + H+ R G+ A K LL Sbjct: 160 --ETHDSAVIEMGMRGRGEIALLAQIAQPTISVITNVGTAHIGRLGSESAIAEAKCELLA 217 Query: 216 R---SEIPVLNADDADIRQHCGH-WSRSCIRW-VSCGDHSPEGGN-PQLTVNAQGWVSRG 269 S I VLN D + + W I + S GD E + L VN + Sbjct: 218 ESPSSSIAVLNYDHPLLTETAKRVWQGETITYGFSGGDIVGELLDLTTLRVNGLDF---- 273 Query: 270 EQHLFPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRC 313 +LPL G H+ N + A A G+ ++++ ++ Sbjct: 274 --------NLPLSGRHHALNFMAALAVAKILGIDWTSLQQGIKV 309 >VL20_313 putative amino acid ligase found clustered with an amidotransferase Length = 445 Score = 38.5 bits (88), Expect = 8e-04, Method: Compositional matrix adjust. Identities = 45/151 (29%), Positives = 66/151 (43%), Gaps = 17/151 (11%) Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNV-------GYSAAELALEVLQGTRPKPD 165 I + GTNGKTT + LL+ +L G A + N G A LA L GT K D Sbjct: 59 ILVVGTNGKTTTSLLLRTILERQG--AKVTHNTTGANLINGLITALLADANLFGTL-KAD 115 Query: 166 WVVMEMSSYQIE-AAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGL---LERSEIPV 221 + ++E+ + + PR + L D L+R+G ++ +G L + + + Sbjct: 116 YAILEVDENILPLVLKDCRPRAILALNLFRDQLDRYGEVDTISRRWQGAISPLPTNTLVI 175 Query: 222 LNADDADIRQHCGHWSRSCIRWVSCGDHSPE 252 LNADD + H G R V G PE Sbjct: 176 LNADDPTL-SHLGQQLPQ--RVVYFGLSEPE 203 >VL20_4628 Dihydrofolate synthase Length = 415 Score = 31.2 bits (69), Expect = 0.16, Method: Compositional matrix adjust. Identities = 15/44 (34%), Positives = 25/44 (56%) Query: 95 RVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLE 138 R++ + + + IP I +TGTNGK +V L +L+ AG + Sbjct: 21 RIKNLLEILGNPQDQIPIIHVTGTNGKGSVCAYLSSILSTAGYK 64 >VL20_2889 Amine oxidase Length = 460 Score = 29.3 bits (64), Expect = 0.73, Method: Compositional matrix adjust. Identities = 15/27 (55%), Positives = 20/27 (74%) Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLE 31 +VVG G +G AAR LQ QG+ V+V+E Sbjct: 12 IVVGAGLAGLIAARNLQRQGHQVLVIE 38 Database: GCA_001264245.1P Posted date: Aug 27, 2016 11:07 PM Number of letters in database: 1,430,872 Number of sequences in database: 5974 Lambda K H 0.319 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 5974 Number of Hits to DB: 1,312,460 Number of extensions: 53540 Number of successful extensions: 195 Number of sequences better than 1.0: 10 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 11 Length of query: 462 Length of database: 1,430,872 Length adjustment: 87 Effective length of query: 375 Effective length of database: 911,134 Effective search space: 341675250 Effective search space used: 341675250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)