BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000760695.2P
7776 sequences; 2,427,444 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DA73_0235780 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synt... 341 e-112
DA73_0224950 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diamin... 60 2e-10
DA73_0210885 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanin... 57 2e-09
DA73_0216040 hypothetical protein 53 3e-09
DA73_0227240 UDP-N-acetylmuramyl peptide synthase 44 3e-05
DA73_0242705 UDP-N-acetylmuramate--alanine ligase 40 7e-04
DA73_0205975 amine oxidase 34 0.030
DA73_0236685 FAD-binding protein 32 0.20
DA73_0207270 3-beta hydroxysteroid dehydrogenase 30 0.57
>DA73_0235780 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Length = 475
Score = 341 bits (875), Expect = e-112, Method: Compositional matrix adjust.
Identities = 209/479 (43%), Positives = 283/479 (59%), Gaps = 35/479 (7%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M R+ V+GLG+SG AARLL+ +G++V + +R P L + L + I ++LG LEL
Sbjct: 1 MPRAHVIGLGKSGVAAARLLKREGWEVELSDRNTSPNLLQQQQELAAEQIAVKLGYSLEL 60
Query: 61 -DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTN 119
S P L +V+SPG+ WD L R G GEM +AW LR IPW+ +TGTN
Sbjct: 61 APSDLPQL-----IVVSPGVPWDISPLLKAREMGIETIGEMELAWRELRSIPWVAVTGTN 115
Query: 120 GKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQG-------------TRPKPDW 166
GKTT T L+ + AGL AP GN+GY+A E+AL + + + + DW
Sbjct: 116 GKTTTTSLIAAIFQKAGLNAPACGNIGYAACEVALSLERNGGRGEGESGGETSIQNQIDW 175
Query: 167 VVMEMSSYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADD 226
V+ E+SSYQIE++ V+PRIGIWTT TPDHL RH T+E Y IK LL++S++ V N DD
Sbjct: 176 VIAELSSYQIESSPSVAPRIGIWTTFTPDHLSRHKTLENYYNIKAHLLQQSQLQVFNGDD 235
Query: 227 ADIRQHCGHWSRSCIRWVSC----------GDHSPEGGNPQLTVNAQGWVSRGEQHLFPA 276
+RQ G S W S G + +G V +G S ++ + A
Sbjct: 236 PYLRQ-VGEKEWSSAYWTSIKGKESLLNSKGYYIEDG----WVVETKGGASSEDERIVEA 290
Query: 277 DSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDS 336
+L + GEHN QN+L+ AAA AG+ I ++R F GVPHRLE + + GI NDS
Sbjct: 291 STLRMVGEHNLQNLLMSVAAARLAGIHKDAIARSVREFPGVPHRLEHICTWEGINFINDS 350
Query: 337 KATNYDAAAVGLRSVQAPVVLLAGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLI 396
KATNYDAA VGL SV++P++L+AGG+ K GD +L I+++ A V+L G A A +
Sbjct: 351 KATNYDAAEVGLASVKSPLILIAGGEAKAGDDTGFLAKIKAKAAFVLLIGNAAPAFAQRL 410
Query: 397 ETSGFAGPMIHCADLDSAVSQALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455
+ G+ + +D AVS++ LA+ QA VLLSPACASFDQY NFE RGDHFR+L
Sbjct: 411 QQQGYESYEV-VETMDRAVSRSAELAKQYQAPVVLLSPACASFDQYANFEQRGDHFRRL 468
>DA73_0224950 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Length = 501
Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 66/247 (26%), Positives = 105/247 (42%), Gaps = 44/247 (17%)
Query: 126 HLLQHVLAHAGLEAPMAGNVGYS---------------AAELALEVLQGTRPKPDWVVME 170
HL++H L A + G + Y+ A EL ++ D+ VME
Sbjct: 126 HLVEHFLNQANFPTALMGTL-YTRWKDFLQTAVHTTPFAVELQQQLAAAIDAGNDYGVME 184
Query: 171 MSSYQIEAAAEVSPR--IGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIP---VLNAD 225
+SS+ + + + +G++T LT DHL+ H +E Y K L + + V+NAD
Sbjct: 185 VSSHALAQGRVMGCQFEVGVFTNLTQDHLDFHRDMEDYFGAKALLFSPNYLKGRAVINAD 244
Query: 226 DADIRQHCGH------WS-----RSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLF 274
D ++ WS R+ W+S ++ P+G V+ +GE
Sbjct: 245 DPYGQRLIASLNSEQVWSYSVSDRNANLWMSNLNYKPDG------VSGMLHTPKGEV--- 295
Query: 275 PADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE--PLGSVGGIPV 332
A PL G+ N +N+L A L G+ I +A+ F GVP R+E + S+ I V
Sbjct: 296 -AFRSPLVGQFNLENLLAAVGAGLHLGLDLQLIASAINEFPGVPGRMERVQISSMQDISV 354
Query: 333 FNDSKAT 339
D T
Sbjct: 355 IVDYAHT 361
>DA73_0210885 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Length = 453
Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 62/213 (29%), Positives = 87/213 (40%), Gaps = 20/213 (9%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPK 163
W IP IG+TG+ GKTT L+ VLA G GN Y+ + L P+
Sbjct: 101 WREQFQIPVIGVTGSVGKTTTKELIAAVLATKGKALKTYGN--YNNEIGVPKTLLQLAPE 158
Query: 164 PDWVVMEMSSYQIEAAAEVS----PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLE---R 216
D+ V+EM+ AE++ P IG+ T + H+E G+ EA K LL +
Sbjct: 159 HDFAVIEMAMRGTGQIAELTHIANPTIGVITNVGTAHIELLGSEEAIARAKCELLAEMPK 218
Query: 217 SEIPVLNAD-DADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLFP 275
+ +LN D I W + + GG+ Q + + G L
Sbjct: 219 DSVAILNHDCPLLIETAAKVWQGKVLTY------GLSGGDIQGRLVDSETIEVGGIQL-- 270
Query: 276 ADSLPLPGEHNRQNMLLVTAAALAAGVSASTIE 308
LPLPG HN N L A A + S +E
Sbjct: 271 --PLPLPGRHNATNFLAALAVAQVLDIDWSFLE 301
>DA73_0216040 hypothetical protein
Length = 80
Score = 53.1 bits (126), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 26/58 (44%), Positives = 38/58 (65%), Gaps = 2/58 (3%)
Query: 402 AGPMIHCADLDSAVSQALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQLINAV 459
A ++ +D AV QA A+ + VLL+PACASFDQ+ N+E RG F+QL++A+
Sbjct: 20 ATEVLQAGTMDVAVRQAYERAQA--GDVVLLAPACASFDQFENYEHRGRVFKQLVHAL 75
>DA73_0227240 UDP-N-acetylmuramyl peptide synthase
Length = 445
Score = 43.9 bits (102), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 71/302 (23%), Positives = 114/302 (37%), Gaps = 48/302 (15%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRP----KPDWVV 168
I I GTNGKTT + LL+ +L G L +L+ T D+ +
Sbjct: 63 ILIAGTNGKTTTSLLLRTILERKGYRVAHNSTGANLENGLMTAMLESTNIVGELDVDYAI 122
Query: 169 MEMSSYQI-EAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKR-----GLLERSEIPVL 222
+E+ I + A + PRI + L D L+R+G ++ KR L R + +
Sbjct: 123 LEVDENIIPKVLAPIQPRIVLCLNLFRDQLDRYGEVDVIS--KRWTKVISTLPRETVVIP 180
Query: 223 NADDADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQL--------------TVNAQG-WVS 267
NADD + + + + G + PE ++ ++N +G ++S
Sbjct: 181 NADDPTLSLLGQQLPQKVLFF---GLNEPENYLEEIPHAVDSIFCPSCGHSLNYKGVYLS 237
Query: 268 RGEQHLFPA----DSLP----------LPGEHNRQNMLLVTAAALAAGVSASTIEAALRC 313
L P S P L G +N+ N L AA+ GV +TI +
Sbjct: 238 HLGDFLCPKCGFQRSQPKLNSNEWSQILVGLYNKYNTLAAATAAIELGVDEATIRDTINN 297
Query: 314 FTGVPHRLEPL---GSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLLAGGQTKKGDAVP 370
F R E L G I + + TN + V +S +L+ + G V
Sbjct: 298 FQAAFGRAEELEINGKRVRILLSKNPVGTN-ETIRVVTQSSDKTTLLVLNDRIPDGTDVS 356
Query: 371 WL 372
W+
Sbjct: 357 WI 358
>DA73_0242705 UDP-N-acetylmuramate--alanine ligase
Length = 488
Score = 39.7 bits (91), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 52/229 (22%), Positives = 87/229 (37%), Gaps = 20/229 (8%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPM--AGNVGYSAAELALEVLQGTRPKPDWVVME 170
I + GT+GKTT + ++ ++L AGL+ + G V + L + ++V E
Sbjct: 138 IAVAGTHGKTTTSSMIGYMLLEAGLDPTILVGGEVDAWSGNARL-------GQSRYLVAE 190
Query: 171 MSSYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIR 230
+ + +P IGI T + DH + + T++ I + V + D +R
Sbjct: 191 ADESD-GSLVKHAPEIGIITNIELDHPDHYETLDEVVEIFQTFARGCTTLVGSIDCETVR 249
Query: 231 QHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVS----RGEQHLFPADSLPLPGEHN 286
+ I + D + + + A G + RG+ +L L +HN
Sbjct: 250 DRL----KPTISYSLYSDTNADYTVTNVDYRADGTTALVWERGKT--LGVLNLRLLSKHN 303
Query: 287 RQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
N L A G+ I L F G R E G GI +D
Sbjct: 304 LSNALAAVAVGRLLGLEFGEIAKGLSTFEGARRRFEFRGEANGITFIDD 352
>DA73_0205975 amine oxidase
Length = 503
Score = 34.3 bits (77), Expect = 0.030, Method: Compositional matrix adjust.
Identities = 18/35 (51%), Positives = 21/35 (60%)
Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36
R VVVG G +G GA L QGY+V +LE G P
Sbjct: 8 KRVVVVGAGWAGLGATYHLAKQGYEVTLLEAGSYP 42
>DA73_0236685 FAD-binding protein
Length = 484
Score = 31.6 bits (70), Expect = 0.20, Method: Compositional matrix adjust.
Identities = 16/32 (50%), Positives = 22/32 (68%)
Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36
+V+G G +GS AA LL +G+DV +LER P
Sbjct: 11 LVIGGGPAGSTAATLLAREGFDVTLLERDFFP 42
>DA73_0207270 3-beta hydroxysteroid dehydrogenase
Length = 330
Score = 30.0 bits (66), Expect = 0.57, Method: Compositional matrix adjust.
Identities = 22/63 (34%), Positives = 28/63 (44%), Gaps = 4/63 (6%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M V G G+ RLL QGY V L R A + L N+T+ G L++
Sbjct: 1 MKAFVTGSTGLLGNNLVRLLLQQGYSVKALVRSSAKA----SQVLSGLNVTLVKGDLLDI 56
Query: 61 DSF 63
DSF
Sbjct: 57 DSF 59
Database: GCA_000760695.2P
Posted date: Aug 27, 2016 10:27 PM
Number of letters in database: 2,427,444
Number of sequences in database: 7776
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 7776
Number of Hits to DB: 2,230,494
Number of extensions: 94832
Number of successful extensions: 316
Number of sequences better than 1.0: 11
Number of HSP's gapped: 306
Number of HSP's successfully gapped: 12
Length of query: 462
Length of database: 2,427,444
Length adjustment: 91
Effective length of query: 371
Effective length of database: 1,719,828
Effective search space: 638056188
Effective search space used: 638056188
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)