BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000482245.1P
7223 sequences; 2,256,402 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
N836_09640 udp-n-acetylmuramoylalanine--d-glutamate ligase 333 e-109
N836_01945 udp-n-acetylmuramoylalanyl-d-glutamate-- -diaminopime... 47 3e-06
N836_18380 udp-n-acetylmuramate--l-alanine ligase 47 4e-06
N836_10290 udp-n-acetylmuramoyl-tripeptide--d-alanyl-d- alanine ... 37 0.003
N836_27685 amine oxidase 35 0.017
N836_10825 cytochrome b6 32 0.13
N836_31160 c-3 desaturase 30 0.64
>N836_09640 udp-n-acetylmuramoylalanine--d-glutamate ligase
Length = 459
Score = 333 bits (854), Expect = e-109, Method: Compositional matrix adjust.
Identities = 205/457 (44%), Positives = 270/457 (59%), Gaps = 25/457 (5%)
Query: 4 SVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLELDSF 63
+ V+GLG SG AARLL+ +G+ VVV +R + L + I +QLG D
Sbjct: 15 AYVIGLGLSGVAAARLLKKEGWRVVVSDRNSGERQVAQQQTLTAEGIDVQLGHNFSAD-- 72
Query: 64 TPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKTT 123
D VV+SPG+ WD +L R +G GEM +AW AL +PW+GITGTNGKTT
Sbjct: 73 ----DPMDLVVVSPGVPWDLPSLVQARENGISTIGEMELAWRALSHLPWLGITGTNGKTT 128
Query: 124 VTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEVS 183
T L+ + A L AP GN+GY+A ++AL+ P+PDWV+ E+SSYQIEAA
Sbjct: 129 TTELVAAIFKSAQLNAPACGNIGYAACQVALD------PEPDWVLAELSSYQIEAAIGPK 182
Query: 184 PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWSRSCIRW 243
P+IG+WTT TPDHL RH TIE Y AIK LLERSE VLN DD +R+ + RW
Sbjct: 183 PKIGVWTTFTPDHLNRHKTIENYYAIKASLLERSEKRVLNGDDPYLRETLAN------RW 236
Query: 244 VSCGDHSPEGGN------PQLTVNAQGWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAA 297
+ S +G N P ++ QGWV + + DSL + G HN QN+L+ AA
Sbjct: 237 ENSYWTSIDGPNAITPLVPSTYIDQQGWVVYQGERILAVDSLKMVGRHNLQNLLMAVTAA 296
Query: 298 LAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVL 357
A + + I AA+ F+GV HRLE + + G+ NDSKATNYDAA VGL SV +PV+L
Sbjct: 297 KLAEIPNAAIAAAIENFSGVAHRLEYVTTWEGVDFINDSKATNYDAAEVGLSSVNSPVIL 356
Query: 358 LAGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCADLDSAVSQ 417
+AGG K+GD WL IQ + V+L GE A + + + G+ G +++AV +
Sbjct: 357 IAGGAPKEGDDNTWLDRIQEKAVQVLLIGEAADQFSQRLIDVGY-GRFEDVGTMENAVQR 415
Query: 418 ALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQ 454
+ +A +A VLLSPACASFDQY NFEARGD F+
Sbjct: 416 SKEIALGFRAKVVLLSPACASFDQYANFEARGDDFKN 452
>N836_01945 udp-n-acetylmuramoylalanyl-d-glutamate-- -diaminopimelate ligase
Length = 502
Score = 47.0 bits (110), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 47/164 (28%), Positives = 72/164 (43%), Gaps = 12/164 (7%)
Query: 168 VMEMSSYQIEAAAE--VSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIP---VL 222
VME+SS+ ++ + ++T LT DHL+ H + Y K L + ++
Sbjct: 186 VMEVSSHALDQKRVWGCPFAVSVFTNLTQDHLDYHKNLNDYFEAKALLFSPDYLKGRAII 245
Query: 223 NADDADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLFPADSLP-- 280
N+ D ++ S + D + + LT +A G RG + P S P
Sbjct: 246 NSRDPYGQKLINRLSPEQVWTYHIDDLAADLHTTDLTYSAAG--VRGSL-ITPMGSAPFT 302
Query: 281 --LPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322
L G N +N+L A L GVS ++ AAL F GVP R+E
Sbjct: 303 SPLVGRFNLENLLAAAGAVLHLGVSLESLIAALAAFGGVPGRME 346
>N836_18380 udp-n-acetylmuramate--l-alanine ligase
Length = 481
Score = 46.6 bits (109), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 55/235 (23%), Positives = 93/235 (39%), Gaps = 32/235 (13%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMS 172
I I GT+GKTT + ++ ++L AG++ + +G A GT D++V E
Sbjct: 121 IAIAGTHGKTTTSSMVGYLLLKAGIDPSIV--IGGEVATWQGNAYVGT---SDYMVAEAD 175
Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232
+ ++S +GI T + DH + + T++ I + ++ ++ + +AD +
Sbjct: 176 ESD-GSLVKLSAAMGIITNIELDHPDHYSTLDEVVGIFKHFAQQCDVVIGSADCETVLSQ 234
Query: 233 CGHWSRSCIRWVSCGDHSPEGGNPQLTVN----AQGWVSR--------GEQHLFPADSLP 280
+R PE G + + AQG + GE HL
Sbjct: 235 IQPTITYSLR--------PESGADYIATDICETAQGTTAEIWERGQRIGELHL------A 280
Query: 281 LPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
+ G HN N L A A GV I + + F G R + G I +D
Sbjct: 281 ILGRHNLSNALAAIAVARRLGVEFDAIASIMATFKGAKRRFDHRGFHNNILFVDD 335
>N836_10290 udp-n-acetylmuramoyl-tripeptide--d-alanyl-d- alanine ligase
Length = 448
Score = 37.4 bits (85), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 55/205 (26%), Positives = 85/205 (41%), Gaps = 39/205 (19%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEV-----LQ 158
W G+P + ITG+ GKTT L+ L + G+V + A E+ L
Sbjct: 103 WRQQLGLPVVAITGSVGKTTTKELISAALG-------LHGSVHKTRANFNNEIGVPKTLL 155
Query: 159 GTRPKPDWVVMEMSSY---QIEAAAEVS-PRIGIWTTLTPDHLERHGTIEAYRAIKRGLL 214
D+ V+EM QI A+++ P + + T + H+ G+ +A K LL
Sbjct: 156 ELTSDHDYAVVEMGMRGPGQIALLAQIAQPNVSVITNVGTAHIGLLGSEQAIADAKCELL 215
Query: 215 ERSEIP-----VLNADDADIRQHCGH-WSRSCIRW-VSCGD-HSPEGGNPQLTVNAQGWV 266
+ +P VLN D+A + WS + I + + GD H GN + + +
Sbjct: 216 --THMPADGTAVLNHDNARLMDTAARVWSGNTITYGLMGGDVHGHFQGNTVVVDDIE--- 270
Query: 267 SRGEQHLFPADSLPLPGEHNRQNML 291
P LPL G+HN N L
Sbjct: 271 -------LP---LPLEGQHNALNYL 285
>N836_27685 amine oxidase
Length = 507
Score = 35.0 bits (79), Expect = 0.017, Method: Compositional matrix adjust.
Identities = 17/35 (48%), Positives = 22/35 (62%)
Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36
+ +VVG G +G GA L QGYDV +LE G +P
Sbjct: 10 KKVIVVGAGWAGLGATYHLAKQGYDVTLLEAGPQP 44
>N836_10825 cytochrome b6
Length = 302
Score = 32.0 bits (71), Expect = 0.13, Method: Compositional matrix adjust.
Identities = 22/90 (24%), Positives = 44/90 (48%), Gaps = 12/90 (13%)
Query: 48 QNITIQLGCPLELDSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDAL 107
Q + + LG L FT W+ G + + ++W L + +R++ E+++ L
Sbjct: 176 QIVVMFLGREFLLSWFTAWISGIGLALTAMALSWTAVVLAWEQTGFWRLKIELNI----L 231
Query: 108 RGIPWIG------ITGTNGKTTVTHLLQHV 131
+PW+G ++G +G ++ T LQH+
Sbjct: 232 GSLPWVGPTLRDILSGGSGISSFT--LQHM 259
>N836_31160 c-3 desaturase
Length = 508
Score = 30.0 bits (66), Expect = 0.64, Method: Compositional matrix adjust.
Identities = 19/53 (35%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLG 55
+ +V+G G G AA LL +GY V V E+ P A R+Q T +G
Sbjct: 15 KVLVIGAGIGGLTAAALLAKRGYRVCVFEQALVPG--GCASTFRRQGFTFDVG 65
Database: GCA_000482245.1P
Posted date: Aug 27, 2016 11:03 PM
Number of letters in database: 2,256,402
Number of sequences in database: 7223
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 7223
Number of Hits to DB: 2,176,756
Number of extensions: 95595
Number of successful extensions: 338
Number of sequences better than 1.0: 7
Number of HSP's gapped: 330
Number of HSP's successfully gapped: 7
Length of query: 462
Length of database: 2,256,402
Length adjustment: 91
Effective length of query: 371
Effective length of database: 1,599,109
Effective search space: 593269439
Effective search space used: 593269439
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)