cyanobase / GCA_000153285.1 - WH7805

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase (462 letters) Database: GCA_000317635.1P 3708 sequences; 1,181,058 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PCC7418_1822 UDP-N-acetylmuramoylalanine--D-glutamate ligase 343 e-114 PCC7418_0721 UDP-N-acetylmuramate--L-alanine ligase 64 6e-12 PCC7418_0074 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diamin... 60 1e-10 PCC7418_0559 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanin... 48 7e-07 PCC7418_0913 protein of unknown function DUF1727 42 7e-05 E4ba2749f1f54a35315406d8bfd517d7

>PCC7418_1822 UDP-N-acetylmuramoylalanine--D-glutamate ligase Length = 451 Score = 343 bits (881), Expect = e-114, Method: Compositional matrix adjust. Identities = 193/455 (42%), Positives = 283/455 (62%), Gaps = 14/455 (3%) Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLELDS 62 ++ V+GLGRSG AARLL+ QG+DV++ +RG +L+ L Q+ IT++L LD+ Sbjct: 2 KAYVIGLGRSGVAAARLLKQQGWDVMISDRGMSDSLQGLQRDLAQEEITVKLQETPTLDN 61 Query: 63 FTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKT 122 L +++SPG+ WD L R + GE+ +AW +L +PW+GITGTNGKT Sbjct: 62 ----LKKTDLIIVSPGVPWDVPILVRAREENIPMYGEIELAWRSLSDVPWVGITGTNGKT 117 Query: 123 TVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEV 182 T T L + +GL AP GN+G +A EL L+ DW++ E+SSYQIE++ ++ Sbjct: 118 TTTALCSALFQGSGLNAPSCGNIGNAACELGLK-----ETSWDWIIAEISSYQIESSQKL 172 Query: 183 SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWSRSCIR 242 +P IG+WTT +PDHLERH T+E Y AIK+ LL+R+++ +LN DD +R + + + S + Sbjct: 173 APEIGVWTTFSPDHLERHYTLENYFAIKKSLLDRAQLKILNGDDPYLR-YLVNENWSGVH 231 Query: 243 WVSCGDHSPEGGNPQLTVNAQG--WVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAALAA 300 W S G P + +G ++ GE+ + P+ G+HN QN+LL AA A Sbjct: 232 WTSIKGKDQLLGKPSHGIYLEGNEIIAFGEK-IIQVSEFPMAGDHNLQNLLLAVGAARLA 290 Query: 301 GVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLLAG 360 GV S I ++ F GVPHRLEP+ + +P NDSKATNY+AA VGL SV +PV+L+AG Sbjct: 291 GVEKSAIAQSIINFQGVPHRLEPICKIENVPFINDSKATNYEAATVGLSSVPSPVILIAG 350 Query: 361 GQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCADLDSAVSQALS 420 GQ K G+ W +LI+++ + V+L GE A + A ++ G+ + +++AV + ++ Sbjct: 351 GQAKTGNDQDWCELIKNKASVVLLIGEAAPKFAQRLDEMGYTSYQV-VETMENAVKEGIN 409 Query: 421 LARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455 + + + VLLSPACASFDQY NFE RGDHFRQL Sbjct: 410 RVKKQEGSVVLLSPACASFDQYDNFEQRGDHFRQL 444 >PCC7418_0721 UDP-N-acetylmuramate--L-alanine ligase Length = 480 Score = 64.3 bits (155), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 74/273 (27%), Positives = 115/273 (42%), Gaps = 18/273 (6%) Query: 68 DSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKTTVTHL 127 D +V+ S I D+ A A G + V + I + GT+GKTT + L Sbjct: 86 DCLPQVICSTAIHPDNSEYQAALARGCPIFHRSDVLAGLVAEYESIAVAGTHGKTTTSSL 145 Query: 128 LQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEVSPRIG 187 L +VL AG++ + +A E V +G ++V E + ++ P IG Sbjct: 146 LGYVLLEAGIDPTVVVGGEVAAWEGNARVGEGR-----YLVAEADESD-GSLVKLKPAIG 199 Query: 188 IWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCG---HWSRSCIRWV 244 I T + DH + + ++ A I + E++E + D +R+ +S C + Sbjct: 200 IVTNIELDHPDHYQSLSAVVDIFQTFAEQTETLIGCIDCETVRETLQPDISYSLDCNQGA 259 Query: 245 --SCGDHSPEGGNPQLTVNAQGWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAALAAGV 302 + D + G + TV QG + GE +L L G HN N L V AAA G+ Sbjct: 260 DYTVTDITYHGQGAKATVWEQGK-ALGELNL------KLLGCHNLSNALAVIAAARKIGI 312 Query: 303 SASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335 I +A+ FTG R E G GI +D Sbjct: 313 EFEVIASAIAQFTGAKRRFEIYGEENGILFVDD 345 >PCC7418_0074 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase Length = 495 Score = 60.1 bits (144), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 65/223 (29%), Positives = 95/223 (42%), Gaps = 32/223 (14%) Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYSA---------AELALEVLQGTRPKPDWVVMEM 171 HL++ L+ A + G + GY+ AEL ++ ++ V+E+ Sbjct: 126 HLIEFFLSVAKKSTALFGTLYARWQGYNQVATHTTPFPAELQAQLATALSAGNEYAVLEV 185 Query: 172 SSYQIEAAAEV---SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI----PVLNA 224 SS+ + A V + + ++T LT DHL+ H T+E Y K LL R E ++N Sbjct: 186 SSHAL-AQGRVKGCAFEVAVFTNLTQDHLDFHETMENYFQAK-ALLFRKEYLRDRAIINQ 243 Query: 225 DDADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQG-----WVSRGEQHLFPADSL 279 DD ++ + + S D S + L A G +GE Sbjct: 244 DDPYGQRLIQELATEKVWRYSRQDTSADLYTENLVYEASGVQGTLKTPQGEITF----RS 299 Query: 280 PLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322 PL GE N N+L AAL GV TI AL FTGVP R+E Sbjct: 300 PLVGEFNLSNLLAAVGAALHLGVELETIAQALPNFTGVPGRME 342 >PCC7418_0559 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase Length = 452 Score = 48.1 bits (113), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 59/222 (26%), Positives = 91/222 (40%), Gaps = 28/222 (12%) Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAG----LEAPMAGNVGYSAAELALEVLQG 159 W IP IGITG+ GKTT L+ VL+ G EA +G L L Sbjct: 104 WRKQYQIPVIGITGSVGKTTTKELIAGVLSQYGKVLKTEANENNEIGVPKTLLRL----- 158 Query: 160 TRPKPDWVVMEMSSYQIEAAAEVS----PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLE 215 D+ V+EM+ AE++ P +G+ T + H+ R G+ +A K LL+ Sbjct: 159 -NSDYDYAVVEMAMRARGEIAELTEIAQPTMGLITNVGTAHIGRLGSRQAIAQAKCELLQ 217 Query: 216 R---SEIPVLNADDAD-IRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQ 271 + +LN D+ I W + + EGG+ Q + ++ + Sbjct: 218 QMPEDATAILNHDNTRLINTAATVWHGKTLTY------GLEGGDLQGRLLDSETLAVAD- 270 Query: 272 HLFPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRC 313 FP LPLPG HN N L A A + + ++ ++ Sbjct: 271 FTFP---LPLPGVHNASNYLAALAVAKTLNLDLTPLQQGIQV 309 >PCC7418_0913 protein of unknown function DUF1727 Length = 446 Score = 41.6 bits (96), Expect = 7e-05, Method: Compositional matrix adjust. Identities = 97/410 (23%), Positives = 158/410 (38%), Gaps = 86/410 (20%) Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAP---MAGNV--GYSAAELALEVLQGTRPKPDWV 167 I I GTNGKTT + LL+ +L G + N+ G A LA L G R D+ Sbjct: 59 ILIVGTNGKTTTSLLLRSILEGNGAKVTHNATGANLINGLITALLADTNLLG-RLNTDYA 117 Query: 168 VMEMSSYQIE-AAAEVSPRIGIWTTLTPDHLERHGTIEAY-----RAIKRGLLERSEIPV 221 ++E+ E P I + L D L+R+G ++ AI R L +S + Sbjct: 118 ILEVDENIFPLLLKECDPTIILGLNLFRDQLDRYGEVDTISEKWQSAIAR--LSQSTTVI 175 Query: 222 LNADDADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQL-------------------TVNA 262 LN DD + H G + + + PQL ++N Sbjct: 176 LNGDDPTL-CHLGQSLPQTVLFFGLNE-------PQLYLEEIPHAVDSIYCPVCGASLNY 227 Query: 263 QG-WVSR-GEQHLFPADSLP-------------LPGEHNRQNMLLVTAAALAAGVSASTI 307 QG ++S G+ H D L G +N+ N L A +S S I Sbjct: 228 QGVYLSHLGDYHCSSCDFTKVKLDINSQDWGQILVGVYNKYNTLAAALTAQTLDISQSLI 287 Query: 308 EAALRCFTGVPHRLEPLGSVGGIPV----------FNDSKATNYDAAAVGLRSVQAPVVL 357 + + F R E L ++ G PV N++ T + A G QA VL Sbjct: 288 QYKIETFRAAFGRAEEL-NIKGTPVRILLSKNPVGMNETIRTVNEIKAQG----QAETVL 342 Query: 358 LA-GGQTKKGDAVPWL------KLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD 410 L + G V W+ KL+ +Q +V+ G+ ++A I+ ++ +++ + Sbjct: 343 LVLNDRAPDGTDVSWIWDVDTEKLV-AQGGTIVISGDRVYDMALRIQ---YSQKVLNYPN 398 Query: 411 LDSAVSQALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQLINAVK 460 L+ + + L A + + +P + P + A + RQL+ K Sbjct: 399 LNLIIKENLKQAVDTALKA---TPPQHTLHILPTYSAMLE-VRQLLTGRK 444 Database: GCA_000317635.1P Posted date: Aug 27, 2016 10:19 PM Number of letters in database: 1,181,058 Number of sequences in database: 3708 Lambda K H 0.319 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3708 Number of Hits to DB: 1,096,456 Number of extensions: 46946 Number of successful extensions: 170 Number of sequences better than 1.0: 8 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 8 Length of query: 462 Length of database: 1,181,058 Length adjustment: 86 Effective length of query: 376 Effective length of database: 862,170 Effective search space: 324175920 Effective search space used: 324175920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.6 bits)