BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000317495.1P
4752 sequences; 1,515,269 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Cri9333_4291 UDP-N-acetylmuramoylalanine--D-glutamate ligase 348 e-115
Cri9333_1207 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanin... 52 7e-08
Cri9333_2335 UDP-N-acetylmuramate--L-alanine ligase 49 3e-07
Cri9333_0078 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diamin... 44 1e-05
Cri9333_4498 amine oxidase 37 0.003
Cri9333_2688 C-3',4' desaturase CrtD 30 0.57
Cri9333_3761 FAD dependent oxidoreductase 30 0.63
>Cri9333_4291 UDP-N-acetylmuramoylalanine--D-glutamate ligase
Length = 449
Score = 348 bits (894), Expect = e-115, Method: Compositional matrix adjust.
Identities = 201/457 (43%), Positives = 272/457 (59%), Gaps = 16/457 (3%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M + V+G+G+SG AARLL+ G++V + +R +L ++ L + I ++LG
Sbjct: 1 MPSAHVIGIGKSGIAAARLLKRDGWEVTLSDRNSSESLLNQQQQLEAEGIAVKLGY---- 56
Query: 61 DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNG 120
SF P S +V+SPG+ WD L R G GEM +AW +L+ PW+ ITGTNG
Sbjct: 57 -SFAP-TSSIDLIVVSPGVPWDIPALVEARNMGIETIGEMELAWRSLQSCPWVAITGTNG 114
Query: 121 KTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAA 180
KTT T L+ + AGL AP GN+G++A ELAL PDW+V E+SSYQIE++
Sbjct: 115 KTTTTSLIAAIFQAAGLNAPACGNIGFAACELAL-----ADTPPDWIVAELSSYQIESSP 169
Query: 181 EVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQ-HCGHWSRS 239
V+P+IG+WTT TPDHL RH T+E Y IK LL +S+ + N DDA + + W+ +
Sbjct: 170 SVAPQIGVWTTFTPDHLSRHKTLENYYNIKAHLLRQSKHQIFNGDDAYLHEIGTKQWADA 229
Query: 240 CIRWVSCGDHSPEGGNPQLTVNAQ-GWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAAL 298
C W S + GNP L + GWV + + A +L + G+HN QN+L+ AAA
Sbjct: 230 C--WTSVEGKAKLIGNPDLGAYIEDGWVIFQGEQILSASALKMVGDHNLQNLLMAVAAAR 287
Query: 299 AAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLL 358
AG+ I A+ F GVPHRLE + + I NDSKATNYDAA VGL SV +P +L+
Sbjct: 288 LAGIEKDAIAQAISNFPGVPHRLEHICTWEDIDFINDSKATNYDAAQVGLASVDSPAILI 347
Query: 359 AGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCADLDSAVSQA 418
AGG+ K GD WL I++Q A V+L G+ A ++ G+ I +D+AV++
Sbjct: 348 AGGEAKAGDDTAWLDTIKAQAAAVLLIGDATKAFATRLDEVGYPNYEI-VETMDNAVARG 406
Query: 419 LSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455
LA+ A VLLSPACASFDQY NFE RGDHFRQL
Sbjct: 407 AELAKQHNAKVVLLSPACASFDQYQNFEQRGDHFRQL 443
>Cri9333_1207 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Length = 449
Score = 51.6 bits (122), Expect = 7e-08, Method: Compositional matrix adjust.
Identities = 60/206 (29%), Positives = 91/206 (44%), Gaps = 29/206 (14%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAG----LEAPMAGNVGYSAAELALEVLQG 159
W IP I +TG+ GKTT L+ +L+ G +A +G L L
Sbjct: 105 WRNQFSIPVIAVTGSVGKTTTKELIAAILSTKGKVLKTQANYNNEIGVPKTLLELS---- 160
Query: 160 TRPKPDWVVMEMSSY---QIEAAAEV-SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLL- 214
+ ++ V+EM+ +I A++ +P +G+ T + H+ R G+ EA K LL
Sbjct: 161 --SEHNYAVIEMAMRGPGEIAELAQIANPTLGVITNVGTAHIGRLGSEEAIAKAKCELLA 218
Query: 215 ERSE--IPVLNADDADIRQHCGHWSRSCIRW-VSCGDHSPEGGNPQLTVNAQGWVSRGEQ 271
E SE I VLN D+ + + W + + + GD E + + T+ +G
Sbjct: 219 EMSESAIAVLNQDNQLLIETAKIWQGKTLTYGLEGGDIHGELIDSK-TIQVEGM------ 271
Query: 272 HLFPADSLPLPGEHNRQNMLLVTAAA 297
HL LPLPG HN N L A A
Sbjct: 272 HL----PLPLPGRHNALNYLAALAIA 293
>Cri9333_2335 UDP-N-acetylmuramate--L-alanine ligase
Length = 505
Score = 49.3 bits (116), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 59/233 (25%), Positives = 97/233 (41%), Gaps = 28/233 (12%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMS 172
I + GT+GKTT + L+ ++L AG++ + +A E + G+ ++V E
Sbjct: 155 IAVAGTHGKTTTSSLIGYMLLKAGIDPTIVVGGEVNAWEGNARLGAGS-----YLVAEAD 209
Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232
+ A++S IG+ T + DH + + T+E + E+ + ++ D ++ Q
Sbjct: 210 ESD-GSLAKLSAEIGVITNIEMDHPDHYATLEEVIDTFQIFAEKCQ-AIVGCIDCEVVQ- 266
Query: 233 CGHWSRSCIRWVSCGDHSPEGGNPQLTVN----------AQGWVSRGEQHLFPADSLPLP 282
S I+ +PE G TV+ AQ W RG+ + L L
Sbjct: 267 ------SKIKPTISYSLNPETG-ADYTVDCVSFGADGTLAQVW-ERGQ--ILGKMKLKLL 316
Query: 283 GEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
G+HN N L A G+ I A+ F G R E G GI +D
Sbjct: 317 GKHNLSNALAAVAVGRKLGLDFDVIAQAIASFDGAKRRFEVRGHYNGITFVDD 369
>Cri9333_0078 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Length = 493
Score = 44.3 bits (103), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 90/334 (26%), Positives = 131/334 (39%), Gaps = 45/334 (13%)
Query: 29 VLERGDEPALRDKAHALRQQNITIQ-----LGCP-LELDSFTPWLDS----AGEVVISPG 78
+LE + PAL + L+ + Q +G P +D W + A ++SP
Sbjct: 13 LLETPNHPALDAEVKGLKTNSHACQAGDLFIGMPGTRVDGGEFWHSALASGAIAAIVSPQ 72
Query: 79 IAWDHHTLHALRAHGFRVRGEMSVAWDALRGIP-----WIGITGTNGKTTVTHLLQHVLA 133
A H A + + A G P IG+TGTNGKTT THL++ +L
Sbjct: 73 AAQKHPIADACVISAADMTQACAAVATAFYGYPGRQLNLIGVTGTNGKTTTTHLIEFLLN 132
Query: 134 HAGLEAPMAGNV-----GYS---------AAELALEVLQGTRPKPDWVVMEMSSYQIEAA 179
H + G + GY A EL ++ VME+SS+ +
Sbjct: 133 HQQHPTALFGTLYTRWSGYQETATHTTPFAVELQKQLADAIAAGCKMGVMEVSSHALAQG 192
Query: 180 AEVSP--RIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNADDADIRQHCG 234
+ + ++T LT DHL+ H +E Y A K L + V+N DD D +
Sbjct: 193 RVLGCPFEVAVFTNLTQDHLDYHRDMEDYFAAKALLFSPEYLQGRAVINLDD-DYGKRLI 251
Query: 235 HWSRSCIRW-VSCGDHSPEGGNPQLTVNAQG-----WVSRGEQHLFPADSLPLPGEHNRQ 288
S S W S D + L G GE A S PL G++N +
Sbjct: 252 EKSPSEKIWSYSVNDSKADLWTSDLNYGQTGVSGMLHTPAGEM----AFSSPLVGQYNLE 307
Query: 289 NMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322
N+L L G+ ++ AL F GVP R+E
Sbjct: 308 NLLAAVGTILHLGLDLESVVQALPEFVGVPGRME 341
>Cri9333_4498 amine oxidase
Length = 503
Score = 36.6 bits (83), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 18/34 (52%), Positives = 23/34 (67%)
Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36
+ VVVG G +G GA L +QGYDV +LE G+ P
Sbjct: 9 KVVVVGAGWAGLGATYQLASQGYDVTLLEAGNYP 42
>Cri9333_2688 C-3',4' desaturase CrtD
Length = 497
Score = 29.6 bits (65), Expect = 0.57, Method: Compositional matrix adjust.
Identities = 18/55 (32%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGC 56
SR VV+G G G LL +GY V++L++ P A ++Q T +G
Sbjct: 6 SRVVVIGAGIGGLTTGALLARRGYQVLILDQALVPG--GCASTFKRQGFTFDVGA 58
>Cri9333_3761 FAD dependent oxidoreductase
Length = 542
Score = 29.6 bits (65), Expect = 0.63, Method: Compositional matrix adjust.
Identities = 15/41 (36%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKA 42
+R +V+G G G A LL G+ ++LERG ++RD++
Sbjct: 97 TRPIVIGTGPCGLFAGLLLAQMGFRPIILERGK--SVRDRS 135
Database: GCA_000317495.1P
Posted date: Aug 27, 2016 10:24 PM
Number of letters in database: 1,515,269
Number of sequences in database: 4752
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4752
Number of Hits to DB: 1,383,080
Number of extensions: 58443
Number of successful extensions: 191
Number of sequences better than 1.0: 10
Number of HSP's gapped: 183
Number of HSP's successfully gapped: 10
Length of query: 462
Length of database: 1,515,269
Length adjustment: 88
Effective length of query: 374
Effective length of database: 1,097,093
Effective search space: 410312782
Effective search space used: 410312782
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)